SSGCID: Target Page

BumuA.00063.a

ATP phosphoribosyltransferase regulatory subunit

CENTER ID:	BumuA.00063.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00063.a.A1.GE33093	Full length( BumuA.00063.a )		1	382

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00063.a.A1.PS01291	Full length( BumuA.00063.a )		1	382

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1671
RefSeq:	YP_001579657.1
UniProt:	A9AH05

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTWLLPENI ADVLPSEARK IEELRRRLLD RFRSYGYEMV MPPLLEYLES LLTSGGADLR LRTFKLVDQL SGRTLGLRAD ITPQVARIDA HLLNRQGVTR LCYAGHVMHT RPRGLHATRE QIQIGAEIYG HAGLEADLEI QQLMLDALHL AGLSRIRLDL CHAGVLAALL ARDTQAAARG EALYDALSGK DVPLLNELTD DLGADTRAAL RALPHLYGDA SVLDEARQRL PALPEIARAL DDLAQLAAQA KGVEVAIDLA DLRGYAYHSG AMFTAYIDGV PNAIARGGRY DHVGQAYGRA RPATGFSLDL RELARISPVE ARGTAILAPW AQDDALRAAV AALRDAGEVV IQALPGHDHV LDEFACDRSL VERNGAWVVE PR

NT Sequence

ATGTCGACCT GGTTACTTCC CGAGAATATC GCCGACGTAC TGCCGTCGGA AGCGCGCAAG ATCGAGGAGC TGCGCCGCAG GCTGCTCGAT CGCTTCCGTT CGTACGGCTA CGAGATGGTG ATGCCGCCGC TGCTCGAATA TCTCGAGTCG CTGCTGACGA GCGGCGGCGC CGATCTGCGC CTGCGCACCT TCAAGCTGGT CGACCAGCTG TCGGGCCGCA CGCTCGGCCT GCGCGCCGAC ATCACGCCGC AGGTCGCGCG TATCGACGCG CATCTGCTGA ACCGCCAGGG CGTCACGCGC CTCTGCTATG CGGGGCACGT CATGCATACG CGCCCGCGCG GCCTGCACGC GACGCGCGAG CAGATCCAGA TCGGCGCGGA AATCTACGGT CACGCGGGAC TGGAAGCCGA TCTCGAGATC CAGCAGTTGA TGCTCGATGC GTTGCATCTG GCCGGCCTGT CGCGGATCCG CCTCGATCTG TGCCATGCGG GCGTGCTCGC GGCGCTGCTG GCGCGCGACA CGCAGGCGGC CGCGCGCGGC GAGGCGCTCT ACGACGCGCT GTCCGGCAAG GACGTGCCGT TGCTGAACGA ACTGACCGAC GATCTCGGCG CCGACACGCG CGCGGCCCTG CGCGCGCTGC CGCACCTGTA CGGCGACGCG AGCGTGCTCG ACGAGGCGCG CCAGCGCCTG CCGGCGCTGC CCGAGATCGC GCGCGCGCTC GACGATCTCG CGCAGCTCGC CGCGCAGGCG AAGGGCGTGG AAGTCGCGAT CGACCTGGCC GATCTGCGCG GTTACGCGTA CCACAGCGGC GCGATGTTTA CCGCGTACAT CGACGGCGTG CCGAACGCGA TCGCGCGCGG CGGCCGTTAC GACCACGTCG GCCAGGCATA CGGCCGCGCG CGTCCGGCGA CCGGCTTCTC GCTCGACCTG CGCGAGCTCG CGCGGATTTC GCCGGTCGAG GCGCGCGGCA CCGCGATTCT CGCGCCGTGG GCGCAGGACG ATGCGCTGCG CGCGGCCGTG GCCGCGCTGC GCGATGCCGG CGAGGTCGTG ATCCAGGCGC TGCCGGGCCA CGATCACGTG CTCGACGAGT TCGCCTGCGA CCGTTCGCTC GTGGAGCGCA ACGGCGCGTG GGTGGTCGAG CCCCGC

Details for BumuA.00063.a.A1.GE33093

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

tagcggggct cgaccaccca cgcgccgttg cgctccacga gcgaacggtc gcaggcgaac tcgtcgagca cgtgatcgtg gcccggcagc gcctggatca cgacctcgcc ggcatcgcgc agcgcggcca cggccgcgcg cagcgcatcg tcctgcgccc acggcgcgag aatcgcggtg ccgcgcgcct cgaccggcga aatccgcgcg agctcgcgca ggtcgagcga gaagccggtc gccggacgcg cgcggccgta tgcctggccg acgtggtcgt aacggccgcc gcgcgcgatc gcgttcggca cgccgtcgat gtacgcggta aacatcgcgc cgctgtggta cgcgtaaccg cgcagatcgg ccaggtcgat cgcgacttcc acgcccttcg cctgcgcggc gagctgcgcg agatcgtcga gcgcgcgcgc gatctcgggc agcgccggca ggcgctggcg cgcctcgtcg agcacgctcg cgtcgccgta caggtgcggc agcgcgcgca gggccgcgcg cgtgtcggcg ccgagatcgt cggtcagttc gttcagcaac ggcacgtcct tgccggacag cgcgtcgtag agcgcctcgc cgcgcgcggc cgcctgcgtg tcgcgcgcca gcagcgccgc gagcacgccc gcatggcaca gatcgaggcg gatccgcgac aggccggcca gatgcaacgc atcgagcatc aactgctgga tctcgagatc ggcttccagt cccgcgtgac cgtagatttc cgcgccgatc tggatctgct cgcgcgtcgc gtgcaggccg cgcgggcgcg tatgcatgac gtgccccgca tagcagaggc gcgtgacgcc ctggcggttc agcagatgcg cgtcgatacg cgcgacctgc ggcgtgatgt cggcgcgcag gccgagcgtg cggcccgaca gctggtcgac cagcttgaag gtgcgcaggc gcagatcggc gccgccgctc gtcagcagcg actcgagata ttcgagcagc ggcggcatca ccatctcgta gccgtacgaa cggaagcgat cgagcagcct gcggcgcagc tcctcgatct tgcgcgcttc cgacggcagt acgtcggcga tattctcggg aagtaaccag gtcgacatcg aaccaggacc ctgggtctga gcttccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTWLLPEN IADVLPSEAR KIEELRRRLL DRFRSYGYEM VMPPLLEYLE SLLTSGGADL RLRTFKLVDQ LSGRTLGLRA DITPQVARID AHLLNRQGVT RLCYAGHVMH TRPRGLHATR EQIQIGAEIY GHAGLEADLE IQQLMLDALH LAGLSRIRLD LCHAGVLAAL LARDTQAAAR GEALYDALSG KDVPLLNELT DDLGADTRAA LRALPHLYGD ASVLDEARQR LPALPEIARA LDDLAQLAAQ AKGVEVAIDL ADLRGYAYHS GAMFTAYIDG VPNAIARGGR YDHVGQAYGR ARPATGFSLD LRELARISPV EARGTAILAP WAQDDALRAA VAALRDAGEV VIQALPGHDH VLDEFACDRS LVERNGAWVV EPR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgacctgg ttacttcccg agaatatcgc cgacgtactg ccgtcggaag cgcgcaagat cgaggagctg cgccgcaggc tgctcgatcg cttccgttcg tacggctacg agatggtgat gccgccgctg ctcgaatatc tcgagtcgct gctgacgagc ggcggcgccg atctgcgcct gcgcaccttc aagctggtcg accagctgtc gggccgcacg ctcggcctgc gcgccgacat cacgccgcag gtcgcgcgta tcgacgcgca tctgctgaac cgccagggcg tcacgcgcct ctgctatgcg gggcacgtca tgcatacgcg cccgcgcggc ctgcacgcga cgcgcgagca gatccagatc ggcgcggaaa tctacggtca cgcgggactg gaagccgatc tcgagatcca gcagttgatg ctcgatgcgt tgcatctggc cggcctgtcg cggatccgcc tcgatctgtg ccatgcgggc gtgctcgcgg cgctgctggc gcgcgacacg caggcggccg cgcgcggcga ggcgctctac gacgcgctgt ccggcaagga cgtgccgttg ctgaacgaac tgaccgacga tctcggcgcc gacacgcgcg cggccctgcg cgcgctgccg cacctgtacg gcgacgcgag cgtgctcgac gaggcgcgcc agcgcctgcc ggcgctgccc gagatcgcgc gcgcgctcga cgatctcgcg cagctcgccg cgcaggcgaa gggcgtggaa gtcgcgatcg acctggccga tctgcgcggt tacgcgtacc acagcggcgc gatgtttacc gcgtacatcg acggcgtgcc gaacgcgatc gcgcgcggcg gccgttacga ccacgtcggc caggcatacg gccgcgcgcg tccggcgacc ggcttctcgc tcgacctgcg cgagctcgcg cggatttcgc cggtcgaggc gcgcggcacc gcgattctcg cgccgtgggc gcaggacgat gcgctgcgcg cggccgtggc cgcgctgcgc gatgccggcg aggtcgtgat ccaggcgctg ccgggccacg atcacgtgct cgacgagttc gcctgcgacc gttcgctcgt ggagcgcaac ggcgcgtggg tggtcgagcc ccgctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BumuA.00063.a.A1.PS01291

PURIFICATION DATe:	11/22/2011
CONCENTRATION:	39.87mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGACCTGG TTACTTCCCG AGAATATCGC CGACGTACTG CCGTCGGAAG CGCGCAAGAT CGAGGAGCTG CGCCGCAGGC TGCTCGATCG CTTCCGTTCG TACGGCTACG AGATGGTGAT GCCGCCGCTG CTCGAATATC TCGAGTCGCT GCTGACGAGC GGCGGCGCCG ATCTGCGCCT GCGCACCTTC AAGCTGGTCG ACCAGCTGTC GGGCCGCACG CTCGGCCTGC GCGCCGACAT CACGCCGCAG GTCGCGCGTA TCGACGCGCA TCTGCTGAAC CGCCAGGGCG TCACGCGCCT CTGCTATGCG GGGCACGTCA TGCATACGCG CCCGCGCGGC CTGCACGCGA CGCGCGAGCA GATCCAGATC GGCGCGGAAA TCTACGGTCA CGCGGGACTG GAAGCCGATC TCGAGATCCA GCAGTTGATG CTCGATGCGT TGCATCTGGC CGGCCTGTCG CGGATCCGCC TCGATCTGTG CCATGCGGGC GTGCTCGCGG CGCTGCTGGC GCGCGACACG CAGGCGGCCG CGCGCGGCGA GGCGCTCTAC GACGCGCTGT CCGGCAAGGA CGTGCCGTTG CTGAACGAAC TGACCGACGA TCTCGGCGCC GACACGCGCG CGGCCCTGCG CGCGCTGCCG CACCTGTACG GCGACGCGAG CGTGCTCGAC GAGGCGCGCC AGCGCCTGCC GGCGCTGCCC GAGATCGCGC GCGCGCTCGA CGATCTCGCG CAGCTCGCCG CGCAGGCGAA GGGCGTGGAA GTCGCGATCG ACCTGGCCGA TCTGCGCGGT TACGCGTACC ACAGCGGCGC GATGTTTACC GCGTACATCG ACGGCGTGCC GAACGCGATC GCGCGCGGCG GCCGTTACGA CCACGTCGGC CAGGCATACG GCCGCGCGCG TCCGGCGACC GGCTTCTCGC TCGACCTGCG CGAGCTCGCG CGGATTTCGC CGGTCGAGGC GCGCGGCACC GCGATTCTCG CGCCGTGGGC GCAGGACGAT GCGCTGCGCG CGGCCGTGGC CGCGCTGCGC GATGCCGGCG AGGTCGTGAT CCAGGCGCTG CCGGGCCACG ATCACGTGCT CGACGAGTTC GCCTGCGACC GTTCGCTCGT GGAGCGCAAC GGCGCGTGGG TGGTCGAGCC CCGCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA