SSGCID: Target Page

BumuA.00057.m

ABC transporter related

CENTER ID:	BumuA.00057.m
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00057.m.A1.GE33086	Full length( BumuA.00057.m )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.3910
RefSeq:	YP_001583602.1
UniProt:	A0A0H3KSZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSANAELLKV AGLQMRFGGL LAVDGIDFDV RRDEVFAIIG PNGAGKTTVF NCVGGFYRPT AGEVVLDGHR IAGLPSHKIA LKGLVRTFQN IRLFKSLTVV ENLLVAQHRK VKSGLLHGLF ATPAYRRAEK EALERAAVWL DRMGLTAVAN RPAGTLSYGH QRRLEIARCM ITEPRLLMLD EPAAGLNPQE KIELQHLIDK LRREFGVSVL LIEHDMSLVM GVSDRILVME HGRPIVIGTP EAVRNDPRVI KAYLGEE

NT Sequence

ATGAGCGCGA ATGCAGAACT GTTGAAGGTC GCCGGATTGC AGATGCGCTT CGGCGGGTTG CTTGCGGTCG ACGGCATCGA CTTCGACGTG CGTCGTGACG AGGTGTTCGC GATCATCGGG CCGAACGGCG CCGGCAAGAC GACGGTGTTC AACTGCGTCG GCGGCTTCTA CCGGCCGACG GCCGGCGAGG TCGTGCTCGA CGGTCATCGG ATCGCGGGGC TGCCGAGCCA CAAGATCGCG CTGAAGGGCC TCGTGCGCAC GTTCCAGAAC ATCCGGCTGT TCAAGTCGCT GACGGTGGTC GAGAACCTGC TCGTCGCACA GCATCGCAAG GTGAAGTCGG GCCTGCTGCA CGGGCTGTTT GCGACGCCCG CGTATCGCCG CGCGGAGAAG GAAGCGCTCG AGCGCGCGGC CGTATGGCTC GACCGCATGG GGCTGACGGC GGTCGCGAAC CGGCCGGCGG GCACGTTGTC GTACGGGCAC CAGCGGCGTC TCGAAATCGC GCGCTGCATG ATCACCGAGC CGCGTCTGCT GATGCTCGAC GAGCCGGCGG CCGGCCTGAA CCCGCAGGAG AAGATCGAGC TTCAGCATCT GATCGACAAG CTGCGCCGCG AGTTCGGCGT GTCGGTGCTG CTGATCGAGC ACGACATGAG CCTCGTGATG GGCGTGTCGG ACCGCATCCT CGTGATGGAG CACGGCCGGC CGATCGTGAT CGGCACGCCG GAAGCGGTCC GCAACGACCC GCGCGTGATC AAGGCGTATC TGGGGGAAGA G

Details for BumuA.00057.m.A1.GE33086

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSANAELLK VAGLQMRFGG LLAVDGIDFD VRRDEVFAII GPNGAGKTTV FNCVGGFYRP TAGEVVLDGH RIAGLPSHKI ALKGLVRTFQ NIRLFKSLTV VENLLVAQHR KVKSGLLHGL FATPAYRRAE KEALERAAVW LDRMGLTAVA NRPAGTLSYG HQRRLEIARC MITEPRLLML DEPAAGLNPQ EKIELQHLID KLRREFGVSV LLIEHDMSLV MGVSDRILVM EHGRPIVIGT PEAVRNDPRV IKAYLGEX

Validated NT Sequence

attttgtttn nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagc gcgaatgcag aactgttgaa ggtcgccgga ttgcagatgc gcttcggcgg gttgcttgcg gtcgacggca tcgacttcga cgtgcgtcgt gacgaggtgt tcgcgatcat cgggccgaac ggcgccggca agacgacggt gttcaactgc gtcggcggct tctaccggcc gacggccggc gaggtcgtgc tcgacggtca tcggatcgcg gggctgccga gccacaagat cgcgctgaag ggcctcgtgc gcacgttcca gaacatccgg ctgttcaagt cgctgacggt ggtcgagaac ctgctcgtcg cacagcatcg caaggtgaag tcgggcctgc tgcacgggct gtttgcgacg cccgcgtatc gccgcgcgga gaaggaagcg ctcgagcgcg cggccgtatg gctcgaccgc atggggctga cggcggtcgc gaaccggccg gcgggcacgt tgtcgtacgg gcaccagcgg cgtctcgaaa tcgcgcgctg catgatcacc gagccgcgtc tgctgatgct cgacgagccg gcggccggcc tgaacccgca ggagaagatc gagcttcagc atctgatcga caagctgcgc cgcgagttcg gcgtgtcggt gctgctgatc gagcacgaca tgagcctcgt gatgggcgtg tcggaccgca tcctcgtgat ggagcacggc cggccgatcg tgatcggcac gccngaagcg gtccgcaacg acccgcgcgt gatcaaggcg tatctggggg aagantaata aacagcacga acaagttctg cagccaagnt tnncgaggat ccggctgcta acaaagcccg aaaggaanct ganttnnnnn nnnnnnnnnn nnnnaaaaaa aaaaaaaaaa anngnnngnn nngtnnngtn nnnncnnnnn nnnnnnngna nnnananttt nnnnnnnnnn nnnnnnnnnn nnnnnnnatg nnnnnnnnnn nnncnnncna annnncnnnn ancntgnnng nnncannnnn nnncnnnnan naccccngna nggnnnnnnn nnnnnnnnnn ntnnntnncn nnnnnnnccc nnnnnnnnnn ttnnnaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgaat gcagaactgt tgaaggtcgc cggattgcag atgcgcttcg gcgggttgct tgcggtcgac ggcatcgact tcgacgtgcg tcgtgacgag gtgttcgcga tcatcgggcc gaacggcgcc ggcaagacga cggtgttcaa ctgcgtcggc ggcttctacc ggccgacggc cggcgaggtc gtgctcgacg gtcatcggat cgcggggctg ccgagccaca agatcgcgct gaagggcctc gtgcgcacgt tccagaacat ccggctgttc aagtcgctga cggtggtcga gaacctgctc gtcgcacagc atcgcaaggt gaagtcgggc ctgctgcacg ggctgtttgc gacgcccgcg tatcgccgcg cggagaagga agcgctcgag cgcgcggccg tatggctcga ccgcatgggg ctgacggcgg tcgcgaaccg gccggcgggc acgttgtcgt acgggcacca gcggcgtctc gaaatcgcgc gctgcatgat caccgagccg cgtctgctga tgctcgacga gccggcggcc ggcctgaacc cgcaggagaa gatcgagctt cagcatctga tcgacaagct gcgccgcgag ttcggcgtgt cggtgctgct gatcgagcac gacatgagcc tcgtgatggg cgtgtcggac cgcatcctcg tgatggagca cggccggccg atcgtgatcg gcacgccgga agcggtccgc aacgacccgc gcgtgatcaa ggcgtatctg ggggaagagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa