BumuA.00057.k

ABC transporter related

CENTER ID: BumuA.00057.k
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00057.k.A1.GE33084 Full length( BumuA.00057.k ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.3450
RefSeq: YP_001583174.1
UniProt: A0A0H3KU67

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASISMRRVQ KAYGDHAPVI RDVDLEIGEH EFCVFLGPSG CGKSTLLRMI AGLEDLSAGE LSIGGRRVDD VPAAERGVAM VFQSYALFPH MTVYENMAFG LKLARTPKAE IDRKVRDAAR ILQLDALLER KPKALSGGQR QRVAIGRAIV REPGVFLFDE PLSNLDAALR GQTRIEIARL HRQFERASVV YVTHDQTEAM TLADKIVLLH AGADTAQHGS IAQVGAPLEL YHRPASRFVA GFIGSPRMNF LPAVVAAADA QRTTVTLVPS GETFTLARDG AALRAGDAVT LGIRPEHLTL GAAHDSTMLV RDVALVERLG EQTCVHLEQP GGTPLIAKVP GDASVRPGER LPVHAPAAAC HLFTEDGRAL PASATLSVHH YA
NT Sequence
ATGGCCAGCA TCTCGATGCG GCGCGTGCAG AAAGCTTATG GCGACCACGC GCCGGTCATA CGCGACGTCG ATCTCGAGAT CGGCGAGCAC GAGTTCTGCG TGTTCCTCGG TCCGTCCGGC TGCGGGAAGT CGACGCTGCT GCGGATGATC GCCGGCCTCG AGGATTTGAG CGCGGGCGAG CTGTCGATCG GCGGCCGGCG CGTCGACGAC GTACCGGCGG CCGAGCGCGG CGTCGCGATG GTGTTCCAGA GCTACGCGCT GTTTCCGCAC ATGACCGTCT ACGAGAACAT GGCGTTCGGC CTGAAGCTCG CGCGCACGCC AAAAGCGGAG ATCGACCGCA AGGTGCGCGA CGCCGCGCGG ATCCTGCAGC TCGACGCGCT GCTCGAGCGC AAGCCGAAGG CGCTGTCGGG CGGGCAGCGG CAGCGCGTGG CGATCGGCCG CGCGATCGTG CGCGAGCCCG GCGTGTTCCT GTTCGACGAG CCGCTGTCGA ACCTCGACGC GGCATTGCGC GGCCAGACGC GCATCGAGAT CGCGCGGCTG CACCGGCAGT TCGAGCGCGC GAGCGTCGTC TACGTGACGC ACGACCAGAC CGAAGCGATG ACGCTCGCCG ACAAGATCGT GCTGCTGCAT GCCGGCGCCG ACACCGCGCA ACACGGCAGC ATCGCGCAGG TGGGCGCGCC GCTCGAGCTG TACCACCGGC CGGCGAGCCG CTTCGTCGCC GGCTTCATCG GCTCGCCGCG GATGAACTTC CTGCCGGCCG TCGTCGCCGC GGCCGATGCG CAGCGCACGA CGGTCACGCT CGTGCCGTCC GGCGAGACCT TCACACTCGC GCGCGACGGC GCCGCGCTGC GCGCGGGCGA CGCCGTCACG CTCGGCATCC GCCCCGAACA TCTGACGCTC GGCGCCGCGC ACGACTCGAC GATGCTCGTG CGCGACGTCG CGCTCGTCGA GCGTCTCGGC GAGCAGACCT GCGTTCATCT CGAGCAGCCC GGCGGCACGC CGCTGATCGC GAAGGTGCCC GGCGACGCGT CGGTGCGCCC CGGCGAGCGC CTACCCGTGC ATGCACCGGC CGCCGCGTGC CACCTGTTTA CCGAAGACGG CCGCGCGCTG CCTGCGTCCG CCACCCTCTC CGTTCACCAT TACGCA
Details for BumuA.00057.k.A1.GE33084
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV VAISFLHAPV IRDVDLEIGE HEFCVFLGPS GCGKSTLLRM IAGLEDLSAG ELSIGGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARTPKA EIDRKVRDAA RILQLDALLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAR LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SXAQVGAPLE LYHRPASRFV AGFIGSPRMN FLPAVVAAAD AQRTTVTLVP SGETFTLARD GAALRAGDAV TLGIRPEHLT LGAAHDSTML XRDVXSXXXS XXXXRSXXXX XXXXXXXXXX XDPXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcca gcatctcgat gcggcgcgtg gtcgccataa gctttctgca cgcgccggtc atacgcgacg tcgatctcga gatcggcgag cacgagttct gcgtgttcct cggtccgtcc ggctgcggga agtcgacgct gctgcggatg atcgccggcc tcgaggattt gagcgcgggc gagctgtcga tcggcggccg gcgcgtcgac gacgtaccgg cggccgagcg cggcgtcgcg atggtgttcc agagctacgc gctgtttccg cacatgaccg tctacgagaa catggcgttc ggcctgaagc tcgcgcgcac gccaaaagcg gagatcgacc gcaaggtgcg cgacgccgcg cggatcctgc agctcgacgc gctgctcgag cgcaagccga aggcgctgtc gggcgggcag cggcagcgcg tggcgatcgg ccgcgcgatc gtgcgcgagc ccggcgtgtt cctgttcgac gagccgctgt cgaacctcga cgcggcattg cgcggccaga cgcgcatcga gatcgcgcgg ctgcaccggc agttcgagcg cgcgagcgtc gtctacgtga cgcacgacca gaccgaagcg atgacgctcg ccgacaagat cgtgctgctg catgccggcg ccgacaccgc gcaacacggc agcancgcgc aggtgggcgc gccgctcgag ctgtaccacc ggccggcgag ccgcttcgtc gccggcttca tcggctcgcc gcggatgaac ttcctgccgg ccgtcgtcgc cgcggccgat gcgcagcgca cgacggtcac gctcgtgccg tccggcgaga ccttcacact cgcgcgcgac ggcgccgcgc tgcgcgcggg cgacgccgtc acgctcggca tccgccccga acatctgacg ctcggcgccg cgcacgactc gacgatgctc nngcgcgacg tcnnctcgnc gnnnntctcg gnnannannn nncgntcatn tcnngnancc nnngnnngnn nntgnannnn nannnnncnn nnngacccnn nnnnnnnnnn nncnnnnnnn nnnncnnnnn nnnncnnnnn nnnngnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV QKAYGDHAPV IRDVDLEIGE HEFCVFLGPS GCGKSTLLRM IAGLEDLSAG ELSIGGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARTPKA EIDRKVRDAA RILQLDALLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAR LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SIAQVGAPLE LYHRPASRFV AGFIGSPRMN FLPAVVAAAD AQRTTVTLVP SGETFTLARD GAALRAGDAV TLGIRPEHLT LGAAHDSTML VRDVALVERL GEQTCVHLEQ PGGTPLIAKV PGDASVRPGE RLPVHAPAAA CHLFTEDGRA LPASATLSVH HYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccagcatc tcgatgcggc gcgtgcagaa agcttatggc gaccacgcgc cggtcatacg cgacgtcgat ctcgagatcg gcgagcacga gttctgcgtg ttcctcggtc cgtccggctg cgggaagtcg acgctgctgc ggatgatcgc cggcctcgag gatttgagcg cgggcgagct gtcgatcggc ggccggcgcg tcgacgacgt accggcggcc gagcgcggcg tcgcgatggt gttccagagc tacgcgctgt ttccgcacat gaccgtctac gagaacatgg cgttcggcct gaagctcgcg cgcacgccaa aagcggagat cgaccgcaag gtgcgcgacg ccgcgcggat cctgcagctc gacgcgctgc tcgagcgcaa gccgaaggcg ctgtcgggcg ggcagcggca gcgcgtggcg atcggccgcg cgatcgtgcg cgagcccggc gtgttcctgt tcgacgagcc gctgtcgaac ctcgacgcgg cattgcgcgg ccagacgcgc atcgagatcg cgcggctgca ccggcagttc gagcgcgcga gcgtcgtcta cgtgacgcac gaccagaccg aagcgatgac gctcgccgac aagatcgtgc tgctgcatgc cggcgccgac accgcgcaac acggcagcat cgcgcaggtg ggcgcgccgc tcgagctgta ccaccggccg gcgagccgct tcgtcgccgg cttcatcggc tcgccgcgga tgaacttcct gccggccgtc gtcgccgcgg ccgatgcgca gcgcacgacg gtcacgctcg tgccgtccgg cgagaccttc acactcgcgc gcgacggcgc cgcgctgcgc gcgggcgacg ccgtcacgct cggcatccgc cccgaacatc tgacgctcgg cgccgcgcac gactcgacga tgctcgtgcg cgacgtcgcg ctcgtcgagc gtctcggcga gcagacctgc gttcatctcg agcagcccgg cggcacgccg ctgatcgcga aggtgcccgg cgacgcgtcg gtgcgccccg gcgagcgcct acccgtgcat gcaccggccg ccgcgtgcca cctgtttacc gaagacggcc gcgcgctgcc tgcgtccgcc accctctccg ttcaccatta cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa