BumuA.00057.j

ABC transporter related

CENTER ID: BumuA.00057.j
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00057.j.A1.GE33083 Full length( BumuA.00057.j ) 1 251
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00057.j.A1.PW33693 Full length( BumuA.00057.j ) 1 251

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.207
RefSeq: YP_001578284.1
UniProt: A0A0H3KSF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDTTMPILD VRGLAVRYGK VEALHGAAIK VGAGQIVSVI GPNGAGKSTL LNAIMGALPV TGHASGAVVY RGEDVGALPV EQRVARGMCL VPEKRELFGT MTVEDNLVLG AYRRKQAGEA NFLDQLDPVF ALFPRLKERR KQAAGTLSGG ERQMLAVGRA LMGKPDLLML DEPSLGLAPL IVKEIFHIIS ALRGTGVATL LIEQNARAAL QISDYGYVLE TGEFALEGPA AELAQNPRVI ETYLGLARKT A
NT Sequence
ATGACGGACA CGACGATGCC GATTCTCGAC GTGCGCGGGC TGGCGGTTCG GTACGGCAAG GTGGAGGCGC TGCACGGCGC GGCGATCAAG GTGGGGGCGG GCCAGATCGT CAGCGTGATC GGCCCGAATG GCGCCGGCAA ATCGACGCTG CTGAACGCGA TCATGGGCGC GCTGCCGGTG ACGGGCCATG CGTCCGGCGC GGTCGTCTAT CGCGGCGAGG ACGTCGGTGC GCTGCCGGTC GAGCAGCGCG TCGCGCGCGG GATGTGCCTC GTGCCGGAGA AGCGCGAGCT GTTCGGCACG ATGACGGTCG AAGACAACCT CGTGCTCGGC GCCTATCGCC GCAAGCAGGC CGGCGAAGCG AACTTTCTCG ACCAACTCGA TCCGGTGTTC GCGCTGTTTC CGCGATTGAA GGAGCGGCGC AAGCAGGCGG CCGGTACGCT GTCGGGCGGC GAGCGGCAGA TGCTCGCGGT CGGGCGTGCG CTGATGGGCA AGCCGGACCT GCTGATGCTC GACGAGCCGA GCCTCGGGCT TGCGCCGCTG ATCGTGAAGG AGATCTTTCA TATCATCAGC GCGTTGCGCG GCACGGGCGT CGCGACGCTG CTGATCGAGC AGAACGCGCG TGCGGCGCTG CAGATTTCGG ACTATGGCTA CGTGCTGGAG ACCGGCGAAT TTGCGCTTGA AGGGCCGGCA GCGGAACTTG CACAGAATCC GCGCGTGATC GAGACGTACC TCGGGCTCGC AAGGAAGACG GCC
Details for BumuA.00057.j.A1.GE33083
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTMPIL DVRGLAVRYG KVEALHGAAI KVGAGQIVSV IGPNGAGKST LLNAIMGALP VTGHASGAVV YRGEDVGALP VEQRVARGMC LVPEKRELFG TMTVEDNLVL GAYRRKQAGE ANFLDQLDPV FALFPRLKER RKQAAGTLSG GERQMLAVGR ALMGKPDLLM LDEPSLGLAP LIVKEIFHII SALRGTGVAT LLIEQNARAA LQISDYGYVL ETGEFALEGP AAELAQNPRV IETYLGLARK TA
Validated NT Sequence
ttattaggcc gtcttccttg cgagcccgag gtacgtctcg atcacgcgcg gattctgtgc aagttccgct gccggccctt caagcgcaaa ttcgccggtc tccagcacgt agccatagtc cgaaatctgc agcgccgcac gcgcgttctg ctcgatcagc agcgtcgcga cgcccgtgcc gcgcaacgcg ctgatgatat gaaagatctc cttcacgatc agcggcgcaa gcccgaggct cggctcgtcg agcatcagca ggtccggctt gcccatcagc gcacgcccga ccgcgagcat ctgccgctcg ccgcccgaca gcgtaccggc cgcctgcttg cgccgctcct tcaatcgcgg aaacagcgcg aacaccggat cgagttggtc gagaaagttc gcttcgccgg cctgcttgcg gcgataggcg ccgagcacga ggttgtcttc gaccgtcatc gtgccgaaca gctcgcgctt ctccggcacg aggcacatcc cgcgcgcgac gcgctgctcg accggcagcg caccgacgtc ctcgccgcga tagacgaccg cgccggacgc atggcccgtc accggcagcg cgcccatgat cgcgttcagc agcgtcgatt tgccggcgcc attcgggccg atcacgctga cgatctggcc cgcccccacc ttgatcgccg cgccgtgcag cgcctccacc ttgccgtacc gaaccgccag cccgcgcacg tcgagaatcg gcatcgtcgt gtccgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTMPIL DVRGLAVRYG KVEALHGAAI KVGAGQIVSV IGPNGAGKST LLNAIMGALP VTGHASGAVV YRGEDVGALP VEQRVARGMC LVPEKRELFG TMTVEDNLVL GAYRRKQAGE ANFLDQLDPV FALFPRLKER RKQAAGTLSG GERQMLAVGR ALMGKPDLLM LDEPSLGLAP LIVKEIFHII SALRGTGVAT LLIEQNARAA LQISDYGYVL ETGEFALEGP AAELAQNPRV IETYLGLARK TA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggacacg acgatgccga ttctcgacgt gcgcgggctg gcggttcggt acggcaaggt ggaggcgctg cacggcgcgg cgatcaaggt gggggcgggc cagatcgtca gcgtgatcgg cccgaatggc gccggcaaat cgacgctgct gaacgcgatc atgggcgcgc tgccggtgac gggccatgcg tccggcgcgg tcgtctatcg cggcgaggac gtcggtgcgc tgccggtcga gcagcgcgtc gcgcgcggga tgtgcctcgt gccggagaag cgcgagctgt tcggcacgat gacggtcgaa gacaacctcg tgctcggcgc ctatcgccgc aagcaggccg gcgaagcgaa ctttctcgac caactcgatc cggtgttcgc gctgtttccg cgattgaagg agcggcgcaa gcaggcggcc ggtacgctgt cgggcggcga gcggcagatg ctcgcggtcg ggcgtgcgct gatgggcaag ccggacctgc tgatgctcga cgagccgagc ctcgggcttg cgccgctgat cgtgaaggag atctttcata tcatcagcgc gttgcgcggc acgggcgtcg cgacgctgct gatcgagcag aacgcgcgtg cggcgctgca gatttcggac tatggctacg tgctggagac cggcgaattt gcgcttgaag ggccggcagc ggaacttgca cagaatccgc gcgtgatcga gacgtacctc gggctcgcaa ggaagacggc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BumuA.00057.j.A1.PW33693
PURIFICATION DATe: 12/9/2011
CONCENTRATION: 8.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTMPIL DVRGLAVRYG KVEALHGAAI KVGAGQIVSV IGPNGAGKST LLNAIMGALP VTGHASGAVV YRGEDVGALP VEQRVARGMC LVPEKRELFG TMTVEDNLVL GAYRRKQAGE ANFLDQLDPV FALFPRLKER RKQAAGTLSG GERQMLAVGR ALMGKPDLLM LDEPSLGLAP LIVKEIFHII SALRGTGVAT LLIEQNARAA LQISDYGYVL ETGEFALEGP AAELAQNPRV IETYLGLARK TA
Validated NT Sequence
ttattaggcc gtcttccttg cgagcccgag gtacgtctcg atcacgcgcg gattctgtgc aagttccgct gccggccctt caagcgcaaa ttcgccggtc tccagcacgt agccatagtc cgaaatctgc agcgccgcac gcgcgttctg ctcgatcagc agcgtcgcga cgcccgtgcc gcgcaacgcg ctgatgatat gaaagatctc cttcacgatc agcggcgcaa gcccgaggct cggctcgtcg agcatcagca ggtccggctt gcccatcagc gcacgcccga ccgcgagcat ctgccgctcg ccgcccgaca gcgtaccggc cgcctgcttg cgccgctcct tcaatcgcgg aaacagcgcg aacaccggat cgagttggtc gagaaagttc gcttcgccgg cctgcttgcg gcgataggcg ccgagcacga ggttgtcttc gaccgtcatc gtgccgaaca gctcgcgctt ctccggcacg aggcacatcc cgcgcgcgac gcgctgctcg accggcagcg caccgacgtc ctcgccgcga tagacgaccg cgccggacgc atggcccgtc accggcagcg cgcccatgat cgcgttcagc agcgtcgatt tgccggcgcc attcgggccg atcacgctga cgatctggcc cgcccccacc ttgatcgccg cgccgtgcag cgcctccacc ttgccgtacc gaaccgccag cccgcgcacg tcgagaatcg gcatcgtcgt gtccgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDTTMPIL DVRGLAVRYG KVEALHGAAI KVGAGQIVSV IGPNGAGKST LLNAIMGALP VTGHASGAVV YRGEDVGALP VEQRVARGMC LVPEKRELFG TMTVEDNLVL GAYRRKQAGE ANFLDQLDPV FALFPRLKER RKQAAGTLSG GERQMLAVGR ALMGKPDLLM LDEPSLGLAP LIVKEIFHII SALRGTGVAT LLIEQNARAA LQISDYGYVL ETGEFALEGP AAELAQNPRV IETYLGLARK TA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGACACG ACGATGCCGA TTCTCGACGT GCGCGGGCTG GCGGTTCGGT ACGGCAAGGT GGAGGCGCTG CACGGCGCGG CGATCAAGGT GGGGGCGGGC CAGATCGTCA GCGTGATCGG CCCGAATGGC GCCGGCAAAT CGACGCTGCT GAACGCGATC ATGGGCGCGC TGCCGGTGAC GGGCCATGCG TCCGGCGCGG TCGTCTATCG CGGCGAGGAC GTCGGTGCGC TGCCGGTCGA GCAGCGCGTC GCGCGCGGGA TGTGCCTCGT GCCGGAGAAG CGCGAGCTGT TCGGCACGAT GACGGTCGAA GACAACCTCG TGCTCGGCGC CTATCGCCGC AAGCAGGCCG GCGAAGCGAA CTTTCTCGAC CAACTCGATC CGGTGTTCGC GCTGTTTCCG CGATTGAAGG AGCGGCGCAA GCAGGCGGCC GGTACGCTGT CGGGCGGCGA GCGGCAGATG CTCGCGGTCG GGCGTGCGCT GATGGGCAAG CCGGACCTGC TGATGCTCGA CGAGCCGAGC CTCGGGCTTG CGCCGCTGAT CGTGAAGGAG ATCTTTCATA TCATCAGCGC GTTGCGCGGC ACGGGCGTCG CGACGCTGCT GATCGAGCAG AACGCGCGTG CGGCGCTGCA GATTTCGGAC TATGGCTACG TGCTGGAGAC CGGCGAATTT GCGCTTGAAG GGCCGGCAGC GGAACTTGCA CAGAATCCGC GCGTGATCGA GACGTACCTC GGGCTCGCAA GGAAGACGGC CTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA