SSGCID: Target Page

BumuA.00057.i

ABC transporter related

CENTER ID:	BumuA.00057.i
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00057.i.A1.GE33082	Full length( BumuA.00057.i )		1	251

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.200
RefSeq:	YP_001578276.1
UniProt:	A0A0H3KN83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKHSEREERE LSGVESGTPA LEITGLEAWY GESHILHGVD LTVHRGEVVT LLGRNGAGRT TTLRAIMGLT GRRSGSIKVA GNETIGLATH RIAHFGVGYC PEERGIFSSL SCEENLMLPP MIGARENAMS LDDIYAMFPN LASRRQSQGT RLSGGEQQML AVARILRTGA NLLLLDEISE GLAPVIVQAL ARMIVALKSR GYTIVMVEQN FRFAAPLADR FYVMEHGRIV EHFRASELEG KMPVLHDLLG V

NT Sequence

ATGAAGCACA GCGAACGGGA GGAACGCGAG TTGAGCGGTG TCGAAAGCGG CACGCCCGCG CTGGAAATCA CGGGCCTCGA GGCCTGGTAC GGGGAATCGC ACATTCTGCA TGGCGTCGAT CTGACCGTGC ATCGCGGCGA GGTCGTCACG CTGCTCGGCC GCAACGGCGC GGGCCGCACG ACGACGCTGC GCGCGATCAT GGGGCTCACG GGCCGCCGCA GCGGCTCGAT CAAGGTGGCA GGCAACGAGA CGATCGGTCT CGCGACGCAC CGCATCGCGC ATTTCGGTGT CGGCTATTGC CCGGAGGAGC GCGGGATCTT CTCGAGCCTG TCGTGCGAGG AGAACCTGAT GCTGCCACCG ATGATCGGCG CGCGCGAGAA CGCGATGTCG CTCGACGACA TCTACGCGAT GTTTCCGAAC CTCGCCTCGC GGCGGCAGAG CCAGGGCACG CGGCTGTCCG GCGGCGAGCA GCAGATGCTC GCGGTTGCGC GCATCCTGCG CACGGGCGCG AACCTGCTGC TGCTCGACGA GATCTCGGAA GGCCTTGCAC CGGTGATCGT GCAGGCGCTT GCGCGCATGA TCGTCGCGCT GAAGTCGCGC GGCTACACGA TCGTGATGGT CGAACAGAAT TTCCGCTTTG CCGCACCGCT CGCCGACCGC TTCTACGTGA TGGAGCACGG CCGCATCGTC GAACATTTCC GCGCGTCGGA ACTGGAAGGC AAGATGCCGG TCCTGCACGA CCTGCTCGGG GTG

Details for BumuA.00057.i.A1.GE33082

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKHSEREER ELSGVESGTP ALEITGLEAW YGESHILHGV DLTVHRGEVV TLLGRNGAGR TTTLRAIMGL TGRRSGSIKV AGNETIGLAT HRIAHFGVGY CPEERGIFSS LSCEENLMLP PMIGARENAM SLDDIYAMFP NLASRRQSQG TRLSGGEQQM LAVARILRTG ANLLLLDEIS EGLAPVIVQA LARMIVALKS RGYTIVMVEQ NFRFAAPLAD RFYVMEHGRI VEHFRASELE GKMPVLHDLL GV

Validated NT Sequence

ttattacacc ccgagcaggt cgtgcaggac cggcatcttg ccttccagtt ccgacgcgcg gaaatgttcg acgatgcggc cgtgctccat cacgtagaag cggtcggcga gcggtgcggc aaagcggaaa ttctgttcga ccatcacgat cgtgtagccg cgcgacttca gcgcgacgat catgcgcgca agcgcctgca cgatcaccgg tgcaaggcct tccgagatct cgtcgagcag cagcaggttc gcgcccgtgc gcaggatgcg cgcaaccgcg agcatctgct gctcgccgcc ggacagccgc gtgccctggc tctgccgccg cgaggcgagg ttcggaaaca tcgcgtagat gtcgtcgagc gacatcgcgt tctcgcgcgc gccgatcatc ggtggcagca tcaggttctc ctcgcacgac aggctcgaga agatcccgcg ctcctccggg caatagccga caccgaaatg cgcgatgcgg tgcgtcgcga gaccgatcgt ctcgttgcct gccaccttga tcgagccgct gcggcggccc gtgagcccca tgatcgcgcg cagcgtcgtc gtgcggcccg cgccgttgcg gccgagcagc gtgacgacct cgccgcgatg cacggtcaga tcgacgccat gcagaatgtg cgattccccg taccaggcct cgaggcccgt gatttccagc gcgggcgtgc cgctttcgac accgctcaac tcgcgttcct cccgttcgct gtgcttcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagcacagc gaacgggagg aacgcgagtt gagcggtgtc gaaagcggca cgcccgcgct ggaaatcacg ggcctcgagg cctggtacgg ggaatcgcac attctgcatg gcgtcgatct gaccgtgcat cgcggcgagg tcgtcacgct gctcggccgc aacggcgcgg gccgcacgac gacgctgcgc gcgatcatgg ggctcacggg ccgccgcagc ggctcgatca aggtggcagg caacgagacg atcggtctcg cgacgcaccg catcgcgcat ttcggtgtcg gctattgccc ggaggagcgc gggatcttct cgagcctgtc gtgcgaggag aacctgatgc tgccaccgat gatcggcgcg cgcgagaacg cgatgtcgct cgacgacatc tacgcgatgt ttccgaacct cgcctcgcgg cggcagagcc agggcacgcg gctgtccggc ggcgagcagc agatgctcgc ggttgcgcgc atcctgcgca cgggcgcgaa cctgctgctg ctcgacgaga tctcggaagg ccttgcaccg gtgatcgtgc aggcgcttgc gcgcatgatc gtcgcgctga agtcgcgcgg ctacacgatc gtgatggtcg aacagaattt ccgctttgcc gcaccgctcg ccgaccgctt ctacgtgatg gagcacggcc gcatcgtcga acatttccgc gcgtcggaac tggaaggcaa gatgccggtc ctgcacgacc tgctcggggt gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa