SSGCID: Target Page

BumuA.00057.e

Branched-chain amino acid transport system ATP-binding protein

CENTER ID:	BumuA.00057.e
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00057.e.A1.GE33078	Full length( BumuA.00057.e )		1	230

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2710
RefSeq:	YP_001580667.1
UniProt:	A0A0H3KLA3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKIEALNQY YGGSHILRNA TLDAEDGKLT VLLGRNGVGK TTLLRCVMGV VAAKSGTVSW RGASLGTLRP EARVAAGLAY VPQGRDIFSR LTVEENLLVG AASRSARSKV PERIYALFPV LKDMRARRGG DLSGGQQQQL AIGRALMSEP QLLILDEPTE GIQPSIIQDI GRTLRQLVDE TKMTVLLVEQ YYDFARSIAD RYWVMSRGEI VAGGDACDMD ANGVRELIAV

NT Sequence

ATGCTGAAGA TCGAAGCACT GAACCAGTAC TACGGCGGCA GCCATATCCT GCGCAACGCG ACGCTCGATG CCGAGGACGG CAAGCTCACC GTGCTGCTCG GCCGCAACGG CGTCGGCAAA ACGACGCTGC TGCGCTGCGT GATGGGCGTC GTCGCCGCGA AAAGCGGCAC CGTGTCGTGG CGCGGCGCGT CGCTCGGCAC GTTGCGACCG GAGGCGCGCG TGGCCGCCGG GCTCGCCTAC GTGCCGCAGG GCCGCGACAT CTTTTCGCGA TTGACCGTCG AGGAGAACCT GCTCGTCGGC GCGGCGAGCC GCAGCGCGCG CTCGAAAGTG CCCGAGCGCA TCTATGCGCT GTTCCCCGTG CTGAAGGACA TGCGCGCGCG GCGCGGCGGC GACCTGTCGG GCGGACAGCA GCAGCAGCTC GCGATCGGCC GTGCGCTGAT GAGCGAGCCG CAGCTGCTGA TCCTCGACGA GCCGACCGAA GGCATCCAGC CGTCGATCAT CCAGGACATC GGGCGCACGC TGCGTCAGCT CGTCGACGAA ACGAAGATGA CCGTGCTGCT CGTCGAGCAG TATTACGATT TCGCGCGCTC GATCGCGGAC CGCTACTGGG TGATGAGCCG CGGCGAAATC GTCGCGGGCG GCGATGCGTG CGACATGGAT GCGAACGGCG TGCGCGAGCT CATCGCGGTC

Details for BumuA.00057.e.A1.GE33078

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKIEALNQ YYGGSHILRN ATLDAEDGKL TVLLGRNGVG KTTLLRCVMG VVAAKSGTVS WRGASLGTLR PEARVAAGLA YVPQGRDIFS RLTVEENLLV GAASRSARSK VPERIYALFP VLKDMRARRG GDLSGGQQQQ LAIGRALMSE PQLLILDEPT EGIQPSIIQD IGRTLRQLVD ETKMTVLLVE QYYDFARSIA DRYWVMSRGE IVAGGDACDM DANGVRELIA V

Validated NT Sequence

tttntttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgctgaag atcgaagcac tgaaccagta ctacggcggc agccatatcc tgcgcaacgc gacgctcgat gccgaggacg gcaagctcac cgtgctgctc ggccgcaacg gcgtcggcaa aacgacgctg ctgcgctgcg tgatgggcgt cgtcgccgcg aaaagcggca ccgtgtcgtg gcgcggcgcg tcgctcggca cgttgcgacc ggaggcgcgc gtggccgccg ggctcgccta cgtgccgcag ggccgcgaca tcttttcgcg attgaccgtc gaggagaacc tgctcgtcgg cgcggcgagc cgcagcgcgc gctcgaaagt gcccgagcgc atctatgcgc tgttccccgt gctgaaggac atgcgcgcgc ggcgcggcgg cgacctgtcg ggcggacagc agcagcagct cgcgatcggc cgtgcgctga tgagcgagcc gcagctgctg atcctcgacg agccgaccga aggcatccag ccgtcgatca tccaggacat cgggcgcacg ctgcgtcagc tcgtcgacga aacgaagatg accgtgctgc tcgtcgagca gtattacgat ttcgcgcgct cgatcgcgga ccgctactgg gtgatgagcc gcggcgaaat cgtcgcgggc ggcgatgcgt gcgacatgga tgcgaacggc gtgcgcgagc tcatcgcggt ctaataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc cacnnnnnnn nnnnnggcnc nnccnnnngn tgncnngnnn nnnnnngntn gnngannnan ncnnnnnann tcnnnnnnnn ncnnnnnnnc nnnnnnncnn nnnngnannt nnnnannnnn ngcnnnnann nnccgncnng aannaannna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagatc gaagcactga accagtacta cggcggcagc catatcctgc gcaacgcgac gctcgatgcc gaggacggca agctcaccgt gctgctcggc cgcaacggcg tcggcaaaac gacgctgctg cgctgcgtga tgggcgtcgt cgccgcgaaa agcggcaccg tgtcgtggcg cggcgcgtcg ctcggcacgt tgcgaccgga ggcgcgcgtg gccgccgggc tcgcctacgt gccgcagggc cgcgacatct tttcgcgatt gaccgtcgag gagaacctgc tcgtcggcgc ggcgagccgc agcgcgcgct cgaaagtgcc cgagcgcatc tatgcgctgt tccccgtgct gaaggacatg cgcgcgcggc gcggcggcga cctgtcgggc ggacagcagc agcagctcgc gatcggccgt gcgctgatga gcgagccgca gctgctgatc ctcgacgagc cgaccgaagg catccagccg tcgatcatcc aggacatcgg gcgcacgctg cgtcagctcg tcgacgaaac gaagatgacc gtgctgctcg tcgagcagta ttacgatttc gcgcgctcga tcgcggaccg ctactgggtg atgagccgcg gcgaaatcgt cgcgggcggc gatgcgtgcg acatggatgc gaacggcgtg cgcgagctca tcgcggtcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a