SSGCID: Target Page

BumuA.00057.b

ABC transporter related

CENTER ID:	BumuA.00057.b
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00057.b.A1.GE33075	Full length( BumuA.00057.b )		1	250

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2105
RefSeq:	YP_001580083.1
UniProt:	A0A0H3KMS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSGTAVALRA IVLRFGARTV LDGIDLDVAA GERHALIGPN GAGKSTLFNV IGGALRPTRG RVMLHGVELR GRGPVIASRL GVGRSFQQTS AFARLSVFDN LRCAALHAPA ERRRWWNRLC ESRSVDRAAA RVMEDIGLDA RRDTPAADLA YAEQRALDLG IALASGARTL LLDEPTAGMN RDQAARMIAL IRATTQGRTV LMIEHDMDAV FGFAERITVL VRGAIVATGA PEAIRADPAV RAAYLGEGTR

NT Sequence

ATGAGCGGCA CCGCAGTCGC GCTGCGCGCG ATCGTGCTGC GCTTCGGTGC GCGGACGGTG CTCGACGGCA TCGACCTCGA CGTCGCGGCC GGCGAGCGGC ATGCGCTGAT CGGCCCGAAC GGCGCCGGCA AGTCGACGCT GTTCAACGTC ATCGGCGGCG CGCTGCGGCC GACGCGCGGG CGCGTGATGC TGCACGGCGT CGAGTTGCGC GGGCGCGGGC CCGTGATCGC GAGCCGGCTC GGCGTCGGCC GCAGTTTTCA GCAGACGAGC GCGTTCGCGC GCCTGAGCGT GTTCGACAAC CTGCGCTGCG CGGCGCTGCA TGCGCCGGCC GAGCGGCGGC GCTGGTGGAA CCGGCTGTGC GAATCGAGGT CGGTCGATCG CGCGGCCGCG CGCGTGATGG AGGACATCGG CCTCGACGCA CGGCGCGACA CGCCGGCCGC CGACCTCGCC TATGCCGAGC AGCGCGCGCT CGATCTCGGC ATCGCGCTGG CGAGCGGCGC GCGCACGCTG CTGCTCGACG AGCCGACGGC CGGCATGAAT CGCGACCAGG CCGCGCGCAT GATCGCGCTG ATCCGCGCGA CCACGCAGGG GCGCACGGTA TTGATGATCG AGCACGACAT GGACGCGGTG TTCGGCTTCG CGGAACGCAT CACGGTGCTC GTGCGCGGCG CGATCGTCGC GACCGGCGCA CCGGAGGCGA TCCGCGCGGA TCCGGCCGTG CGCGCCGCCT ATCTCGGCGA GGGCACACGA

Details for BumuA.00057.b.A1.GE33075

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSGTAVALR AIVLRFGART VLDGIDLDVA AGERHALIGP NGAGKSTLFN VIGGALRPTR GRVMLHGVEL RGRGPVIASR LGVGRSFQQT SAFARLSVFD NLRCAALHAP AERRRWWNRL CESRSVDRAA ARVMEDIGLD ARRDTPAADL AYAEQRALDL GIALASGART LLLDEPTAGM NRDQAARMIA LIRATTQGRT VLMIEHDMDA VFGFAERITV LVRGAIVATG APXAIRADPA VRAAYLGEGT R

Validated NT Sequence

annnattttg ttnnntttaa gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg agcggcaccg cagtcgcgct gcgcgcgatc gtgctgcgct tcggtgcgcg gacggtgctc gacggcatcg acctcgacgt cgcggccggc gagcggcatg cgctgatcgg cccgaacggc gccggcaagt cgacgctgtt caacgtcatc ggcggcgcgc tgcggccgac gcgcgggcgc gtgatgctgc acggcgtcga gttgcgcggg cgcgggcccg tgatcgcgag ccggctcggc gtcggccgca gttttcagca gacgagcgcg ttcgcgcgcc tgagcgtgtt cgacaacctg cgctgcgcgg cgctgcatgc gccggccgag cggcggcgct ggtggaaccg gctgtgcgaa tcgaggtcgg tcgatcgcgc ggccgcgcgc gtgatggagg acatcggcct cgacgcacgg cgcgacacgc cggccgccga cctcgcctat gccgagcagc gcgcgctcga tctcggcatc gcgctggcga gcggcgcgcg cacgctgctg ctcgacgagc cgacggccgg catgaatcgc gaccaggccg cgcgcatgat cgcgctgatc cgcgcgacca cgcaggggcg cacggtattg atgatcgagc acgacatgga cgcggtgttc ggcttcgcgg aacgcatcac ggtgctcgtg cgcggcgcga tcgtcgcgac cggcgcaccg gangcgatcc gcgcggatcc ggccgtgcgc gccgcctatc tcggcgaggg cacacgataa taaacagcac gaacaagttc tgcagccaag cttctcgang atnccggctg ctancaaagc ccgaaaggaa gctganttgn ctgctgcnnc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncnnca nnnnncnnnn nnannnagcn nnnnnnagnn nnnnnnnnnt nnt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcggcacc gcagtcgcgc tgcgcgcgat cgtgctgcgc ttcggtgcgc ggacggtgct cgacggcatc gacctcgacg tcgcggccgg cgagcggcat gcgctgatcg gcccgaacgg cgccggcaag tcgacgctgt tcaacgtcat cggcggcgcg ctgcggccga cgcgcgggcg cgtgatgctg cacggcgtcg agttgcgcgg gcgcgggccc gtgatcgcga gccggctcgg cgtcggccgc agttttcagc agacgagcgc gttcgcgcgc ctgagcgtgt tcgacaacct gcgctgcgcg gcgctgcatg cgccggccga gcggcggcgc tggtggaacc ggctgtgcga atcgaggtcg gtcgatcgcg cggccgcgcg cgtgatggag gacatcggcc tcgacgcacg gcgcgacacg ccggccgccg acctcgccta tgccgagcag cgcgcgctcg atctcggcat cgcgctggcg agcggcgcgc gcacgctgct gctcgacgag ccgacggccg gcatgaatcg cgaccaggcc gcgcgcatga tcgcgctgat ccgcgcgacc acgcaggggc gcacggtatt gatgatcgag cacgacatgg acgcggtgtt cggcttcgcg gaacgcatca cggtgctcgt gcgcggcgcg atcgtcgcga ccggcgcacc ggaggcgatc cgcgcggatc cggccgtgcg cgccgcctat ctcggcgagg gcacacgata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a