SSGCID: Target Page

BumuA.00057.a

ABC transporter related

CENTER ID:	BumuA.00057.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00057.a.A1.GE33074	Full length( BumuA.00057.a )		1	234

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.2104
RefSeq:	YP_001580082.1
UniProt:	A0A0H3KE78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSALLDIRAL RAWYGHQPVL DGIDLALALG ETLALLGRNG AGRSTLAKAV MGLVRTSGSV RVAGAECVGA RTFDIARRGV AYVAESRDVF PLLSVRDNLL LGLRGVGRAA ARAELERLFA RFPLLAARAD VKAARLSGGE QQVLALVRAL AGRPRVLIVD EPAEGLAPLA VDEVRACLAA LQADGVAILL IEQRLQLAPR LAHRVAVMGR GTIVYDGALD GLGDDTVRAW LGTG

NT Sequence

ATGAGCGCGC TGCTCGACAT TCGTGCGCTG CGCGCGTGGT ACGGACATCA GCCGGTGCTC GACGGCATCG ATCTCGCGCT CGCGCTGGGC GAGACGCTTG CGCTGCTCGG ACGCAACGGC GCCGGCCGCT CGACGCTCGC GAAGGCCGTG ATGGGGCTCG TGCGCACGAG CGGTTCGGTG CGCGTCGCGG GCGCCGAATG CGTGGGCGCA CGTACCTTCG ACATCGCGCG TCGCGGCGTC GCCTACGTCG CCGAAAGCCG CGACGTGTTT CCGCTGCTCA GCGTGCGCGA CAACCTGCTG CTCGGGCTGC GCGGCGTCGG CCGGGCGGCC GCGCGCGCGG AACTCGAGCG GCTGTTCGCA CGCTTTCCGC TGCTCGCCGC GCGCGCCGAC GTGAAGGCCG CGCGGCTGTC GGGCGGCGAA CAGCAGGTGC TCGCGCTCGT GCGTGCGCTC GCCGGCCGCC CGCGCGTGCT GATCGTCGAC GAACCGGCCG AGGGGCTCGC GCCGCTTGCC GTCGACGAAG TGCGCGCGTG CCTCGCCGCG CTGCAGGCGG ACGGCGTCGC GATCCTGCTG ATCGAGCAGC GGTTGCAGCT GGCGCCGCGG CTGGCGCATC GCGTCGCGGT GATGGGGCGC GGAACGATCG TCTACGACGG CGCGCTCGAC GGCCTCGGCG ACGACACCGT GCGCGCCTGG CTCGGCACCG GC

Details for BumuA.00057.a.A1.GE33074

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSALLDIRA LRAWYGHQPV LDGIDLALAL GETLALLGRN GAGRSTLAKA VMGLVRTSGS VRVAGAECVG ARTFDIARRG VAYVAESRDV FPLLSVRDNL LLGLRGVGRA AARAELERLF ARFPLLAARA DVKAARLSGG EQQVLALVRA LAGRPRVLIV DEPAEGLAPL AVDEVRACLA ALQADGVAIL LIEQRLQLAP RLAHRVAVMG RGTIVYDGAL DGLGDDTVRA WLGTG

Validated NT Sequence

ttttnttnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcgc gctgctcgac attcgtgcgc tgcgcgcgtg gtacggacat cagccggtgc tcgacggcat cgatctcgcg ctcgcgctgg gcgagacgct tgcgctgctc ggacgcaacg gcgccggccg ctcgacgctc gcgaaggccg tgatggggct cgtgcgcacg agcggttcgg tgcgcgtcgc gggcgccgaa tgcgtgggcg cacgtacctt cgacatcgcg cgtcgcggcg tcgcctacgt cgccgaaagc cgcgacgtgt ttccgctgct cagcgtgcgc gacaacctgc tgctcgggct gcgcggcgtc ggccgggcgg ccgcgcgcgc ggaactcgag cggctgttcg cacgctttcc gctgctcgcc gcgcgcgccg acgtgaaggc cgcgcggctg tcgggcggcg aacagcaggt gctcgcgctc gtgcgtgcgc tcgccggccg cccgcgcgtg ctgatcgtcg acgaaccggc cgaggggctc gcgccgcttg ccgtcgacga agtgcgcgcg tgcctcgccg cgctgcaggc ggacggcgtc gcgatcctgc tgatcgagca gcggttgcag ctggcgccgc ggctggcgca tcgcgtcgcg gtgatggggc gcggaacgat cgtctacgac ggcgcgctcg acggcctcgg cgacgacacc gtgcgcgcct ggctcggcac cggctaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg anttggctgc tgcancgctn annnaaannc nnnnnnnana annnnntgat nnnnnnnnnn nnnnnntctn nnnnnnnann nnnnacnnnn natnnngngn ncntcnnnct nnnnnnnnan cnncnnnnnn nnnnnnnntn nnnnnncngn nntnnnnnnn nnnnnnnnnn nnnnnnnnnc nnncnnnnnn nnnannncnn nnnggnnnnn nnnnnnnnnn nnnnncccnn ccnannnnnn nnnnnnnnnn nnnnnnnnnn nnccnnnnnn nncnnnntnn nnntnnnnnn nnnnnnnnan nttnnnnnnn nnnnnnnnnn nnnggg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgctg ctcgacattc gtgcgctgcg cgcgtggtac ggacatcagc cggtgctcga cggcatcgat ctcgcgctcg cgctgggcga gacgcttgcg ctgctcggac gcaacggcgc cggccgctcg acgctcgcga aggccgtgat ggggctcgtg cgcacgagcg gttcggtgcg cgtcgcgggc gccgaatgcg tgggcgcacg taccttcgac atcgcgcgtc gcggcgtcgc ctacgtcgcc gaaagccgcg acgtgtttcc gctgctcagc gtgcgcgaca acctgctgct cgggctgcgc ggcgtcggcc gggcggccgc gcgcgcggaa ctcgagcggc tgttcgcacg ctttccgctg ctcgccgcgc gcgccgacgt gaaggccgcg cggctgtcgg gcggcgaaca gcaggtgctc gcgctcgtgc gtgcgctcgc cggccgcccg cgcgtgctga tcgtcgacga accggccgag gggctcgcgc cgcttgccgt cgacgaagtg cgcgcgtgcc tcgccgcgct gcaggcggac ggcgtcgcga tcctgctgat cgagcagcgg ttgcagctgg cgccgcggct ggcgcatcgc gtcgcggtga tggggcgcgg aacgatcgtc tacgacggcg cgctcgacgg cctcggcgac gacaccgtgc gcgcctggct cggcaccggc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa