BumuA.00046.b
Alpha,alpha-trehalose-phosphate synthase
| CENTER ID: | BumuA.00046.b |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00046.b.B1.GE39667 | Full length( BumuA.00046.b ) | 1 | 473 | ||
| BumuA.00046.b.B11.GE39668 | Full length( BumuA.00046.b ) | 1 | 473 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00046.b.B1.PS37880 | Full length( BumuA.00046.b ) | 1 | 473 | ||
| BumuA.00046.b.B11.PS37890 | Full length( BumuA.00046.b ) | 1 | 473 |
Structures
5UOF
DEPOSITED: 1/31/2017
DETERMINATION: XRay
CLONE: BumuA.00046.b.B1.GE39667
PROTEIN: BumuA.00046.b.B1.PS37880
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|395019.8.peg.1056 |
| RefSeq: | YP_001579088.1 |
| UniProt: | A0A0H3KGN3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLIVVSNR VAIGEDTRPS AGGLAVGVMD ALQETGGVWF GWNGEIVGTP DAAPAIRRDG NVTYATVGLT RRDYDQYYRG FSNATLWPVF HYRGDLARFD RQEYAGYLRV NAMLAKQLAA LLRPDDLIWV HDYHLLPFAH ALRELGVKNP IGFFLHIPFP SPDVLRLVPP HDELVKFMCA YDVTGFQTDA DRQAFTDYIE RRGIGTASED GMLHAHGRVV KVAAYPIGVY PDAIAQAAVQ YGARKPVKML RDALGGRKLV MSVDRLDYSK GLVERFQAFE RMLANAPGWQ GRVSLVQIAP PTRSDVQTYQ RIRETLEGEA GRINGRFSQL DWTPIQYLNR KYERNLLMAF FRMSQVGYVT PLRDGMNLVA KEYVASQDPA DPGVLVLSEF AGAAAELTGA LLVNPYDLSQ MADALERALS MPLAERQARH EENLARLRAN DLSVWRDTFV ADLRSVAAAA SVTQRAGRRI AHA
NT Sequence
ATGAGCAGAT TGATCGTGGT ATCGAACCGT GTCGCCATCG GCGAGGACAC ACGCCCGAGC GCGGGCGGCC TCGCGGTCGG CGTGATGGAC GCGCTGCAGG AAACGGGCGG CGTCTGGTTC GGCTGGAACG GCGAGATCGT GGGGACGCCG GACGCGGCGC CGGCGATCCG GCGCGACGGC AACGTGACTT ATGCGACGGT CGGACTGACG CGCCGCGACT ACGATCAGTA CTATCGCGGG TTTTCGAACG CGACGCTGTG GCCCGTGTTC CACTATCGCG GCGATCTCGC GCGCTTCGAC CGACAGGAGT ACGCCGGCTA TCTGCGCGTG AACGCGATGC TCGCGAAGCA GCTCGCCGCA CTGCTGCGCC CGGACGATCT GATCTGGGTG CACGACTACC ATCTGCTGCC GTTCGCGCAC GCGCTGCGCG AGCTCGGCGT GAAGAATCCG ATCGGCTTCT TCCTGCACAT TCCGTTTCCG TCGCCGGACG TGCTGCGGCT CGTGCCGCCG CACGACGAAC TCGTGAAGTT CATGTGCGCG TATGACGTGA CGGGCTTCCA GACCGATGCC GACAGGCAGG CGTTCACCGA CTACATCGAG CGGCGCGGGA TCGGCACCGC AAGCGAGGAC GGCATGCTGC ACGCACACGG CCGCGTCGTG AAGGTGGCCG CGTATCCGAT CGGCGTGTAT CCGGATGCGA TCGCGCAGGC GGCCGTCCAG TACGGCGCGC GCAAGCCGGT GAAGATGCTG CGCGACGCAC TGGGCGGCCG CAAGCTCGTG ATGAGCGTCG ATCGTCTCGA CTATTCGAAG GGGCTCGTCG AACGCTTCCA GGCGTTCGAG CGCATGCTCG CGAATGCGCC GGGCTGGCAG GGGCGCGTGT CGCTCGTGCA GATCGCGCCG CCGACGCGTT CCGACGTGCA GACGTACCAG CGAATCCGCG AAACGCTCGA AGGCGAGGCC GGCCGCATCA ACGGACGGTT TTCGCAGCTC GACTGGACGC CGATTCAATA TCTGAACCGC AAGTACGAGC GCAATCTGCT GATGGCGTTC TTCCGCATGT CGCAGGTCGG CTACGTGACG CCGCTGCGCG ACGGGATGAA CCTCGTCGCG AAGGAATACG TCGCGTCGCA GGATCCGGCC GATCCGGGCG TGCTCGTGCT GTCCGAATTT GCGGGCGCCG CGGCGGAGCT GACGGGCGCG CTGCTCGTCA ATCCGTATGA CCTCTCGCAG ATGGCCGACG CGCTCGAGCG TGCACTTTCG ATGCCGCTCG CGGAACGGCA GGCGCGTCAC GAGGAGAACC TCGCGCGGCT GCGCGCGAAC GACCTGTCGG TGTGGCGCGA TACGTTCGTC GCCGATCTGC GCAGCGTCGC GGCGGCGGCC TCGGTCACGC AGCGCGCGGG CCGACGCATC GCGCATGCG
Details for BumuA.00046.b.B1.GE39667
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLIVVSNRVA IGEDTRPSAG GLAVGVMDAL QETGGVWFGW NGEIVGTPDA APAIRRDGNV TYATVGLTRR DYDQYYRGFS NATLWPVFHY RGDLARFDRQ EYAGYLRVNA MLAKQLAALL RPDDLIWVHD YHLLPFAHAL RELGVKNPIG FFLHIPFPSP DVLRLVPPHD ELVKFMCAYD VTGFQTDADR QAFTDYIERR GIGTASEDGM LHAHGRVVKV AAYPIGVYPD AIAQAAVQYG ARKPVKMLRD ALGGRKLVMS VDRLDYSKGL VERFQAFERM LANAPGWQGR VSLVQIAPPT RSDVQTYQRI RETLEGEAGR INGRFSQLDW TPIQYLNRKY ERNLLMAFFR MSQVGYVTPL RDGMNLVAKE YVASQDPADP GVLVLSEFAG AAAELTGALL VNPYDLSQMA DALERALSMP LAERQARHEE NLARLRANDL SVWRDTFVAD LRSVAAAASV TQRAGRRIAH A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gattgatcgt ggtatcgaac cgtgtcgcca tcggcgagga cacacgcccg agcgcgggcg gcctcgcggt cggcgtgatg gacgcgctgc aggaaacggg cggcgtctgg ttcggctgga acggcgagat cgtggggacg ccggacgcgg cgccggcgat ccggcgcgac ggcaacgtga cttatgcgac ggtcggactg acgcgccgcg actacgatca gtactatcgc gggttttcga acgcgacgct gtggcccgtg ttccactatc gcggcgatct cgcgcgcttc gaccgacagg agtacgccgg ctatctgcgc gtgaacgcga tgctcgcgaa gcagctcgcc gcactgctgc gcccggacga tctgatctgg gtgcacgact accatctgct gccgttcgcg cacgcgctgc gcgagctcgg cgtgaagaat ccgatcggct tcttcctgca cattccgttt ccgtcgccgg acgtgctgcg gctcgtgccg ccgcacgacg aactcgtgaa gttcatgtgc gcgtatgacg tgacgggctt ccagaccgat gccgacaggc aggcgttcac cgactacatc gagcggcgcg ggatcggcac cgcaagcgag gacggcatgc tgcacgcaca cggccgcgtc gtgaaggtgg ccgcgtatcc gatcggcgtg tatccggatg cgatcgcgca ggcggccgtc cagtacggcg cgcgcaagcc ggtgaagatg ctgcgcgacg cactgggcgg ccgcaagctc gtgatgagcg tcgatcgtct cgactattcg aaggggctcg tcgaacgctt ccaggcgttc gagcgcatgc tcgcgaatgc gccgggctgg caggggcgcg tgtcgctcgt gcagatcgcg ccgccgacgc gttccgacgt gcagacgtac cagcgaatcc gcgaaacgct cgaaggcgag gccggccgca tcaacggacg gttttcgcag ctcgactgga cgccgattca atatctgaac cgcaagtacg agcgcaatct gctgatggcg ttcttccgca tgtcgcaggt cggctacgtg acgccgctgc gcgacgggat gaacctcgtc gcgaaggaat acgtcgcgtc gcaggatccg gccgatccgg gcgtgctcgt gctgtccgaa tttgcgggcg ccgcggcgga gctgacgggc gcgctgctcg tcaatccgta tgacctctcg cagatggccg acgcgctcga gcgtgcactt tcgatgccgc tcgcggaacg gcaggcgcgt cacgaggaga acctcgcgcg gctgcgcgcg aacgacctgt cggtgtggcg cgatacgttc gtcgccgatc tgcgcagcgt cgcggcggcg gcctcggtca cgcagcgcgc gggccgacgc atcgcgcatg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BumuA.00046.b.B11.GE39668
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 54kDa |
| OBSERVED MW: | 54kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLIVVSNRVA IGEDTRPSAG GLAVGVMDAL QETGGVWFGW NGEIVGTPDA APAIRRDGNV TYATVGLTRR DYDQYYRGFS NATLWPVFHY RGDLARFDRQ EYAGYLRVNA MLAKQLAALL RPDDLIWVHD YHLLPFAHAL RELGVKNPIG FFLHIPFPSP DVLRLVPPHD ELVKFMCAYD VTGFQTDADR QAFTDYIERR GIGTASEDGM LHAHGRVVKV AAYPIGVYPD AIAQAAVQYG ARKPVKMLRD ALGGRKLVMS VDRLDYSKGL VERFQAFERM LANAPGWQGR VSLVQIAPPT RSDVQTYQRI RETLEGEAGR INGRFSQLDW TPIQYLNRKY ERNLLMAFFR MSQVGYVTPL RDGMNLVAKE YVASQDPADP GVLVLSEFAG AAAELTGALL VNPYDLSQMA DALERALSMP LAERQARHEE NLARLRANDL SVWRDTFVAD LRSVAAAASV TQRAGRRIAH A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca gattgatcgt ggtatcgaac cgtgtcgcca tcggcgagga cacacgcccg agcgcgggcg gcctcgcggt cggcgtgatg gacgcgctgc aggaaacggg cggcgtctgg ttcggctgga acggcgagat cgtggggacg ccggacgcgg cgccggcgat ccggcgcgac ggcaacgtga cttatgcgac ggtcggactg acgcgccgcg actacgatca gtactatcgc gggttttcga acgcgacgct gtggcccgtg ttccactatc gcggcgatct cgcgcgcttc gaccgacagg agtacgccgg ctatctgcgc gtgaacgcga tgctcgcgaa gcagctcgcc gcactgctgc gcccggacga tctgatctgg gtgcacgact accatctgct gccgttcgcg cacgcgctgc gcgagctcgg cgtgaagaat ccgatcggct tcttcctgca cattccgttt ccgtcgccgg acgtgctgcg gctcgtgccg ccgcacgacg aactcgtgaa gttcatgtgc gcgtatgacg tgacgggctt ccagaccgat gccgacaggc aggcgttcac cgactacatc gagcggcgcg ggatcggcac cgcaagcgag gacggcatgc tgcacgcaca cggccgcgtc gtgaaggtgg ccgcgtatcc gatcggcgtg tatccggatg cgatcgcgca ggcggccgtc cagtacggcg cgcgcaagcc ggtgaagatg ctgcgcgacg cactgggcgg ccgcaagctc gtgatgagcg tcgatcgtct cgactattcg aaggggctcg tcgaacgctt ccaggcgttc gagcgcatgc tcgcgaatgc gccgggctgg caggggcgcg tgtcgctcgt gcagatcgcg ccgccgacgc gttccgacgt gcagacgtac cagcgaatcc gcgaaacgct cgaaggcgag gccggccgca tcaacggacg gttttcgcag ctcgactgga cgccgattca atatctgaac cgcaagtacg agcgcaatct gctgatggcg ttcttccgca tgtcgcaggt cggctacgtg acgccgctgc gcgacgggat gaacctcgtc gcgaaggaat acgtcgcgtc gcaggatccg gccgatccgg gcgtgctcgt gctgtccgaa tttgcgggcg ccgcggcgga gctgacgggc gcgctgctcg tcaatccgta tgacctctcg cagatggccg acgcgctcga gcgtgcactt tcgatgccgc tcgcggaacg gcaggcgcgt cacgaggaga acctcgcgcg gctgcgcgcg aacgacctgt cggtgtggcg cgatacgttc gtcgccgatc tgcgcagcgt cgcggcggcg gcctcggtca cgcagcgcgc gggccgacgc atcgcgcatg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BumuA.00046.b.B1.PS37880
| PURIFICATION DATe: | 3/16/2016 |
| CONCENTRATION: | 27.93mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS RLIVVSNRVA IGEDTRPSAG GLAVGVMDAL QETGGVWFGW NGEIVGTPDA APAIRRDGNV TYATVGLTRR DYDQYYRGFS NATLWPVFHY RGDLARFDRQ EYAGYLRVNA MLAKQLAALL RPDDLIWVHD YHLLPFAHAL RELGVKNPIG FFLHIPFPSP DVLRLVPPHD ELVKFMCAYD VTGFQTDADR QAFTDYIERR GIGTASEDGM LHAHGRVVKV AAYPIGVYPD AIAQAAVQYG ARKPVKMLRD ALGGRKLVMS VDRLDYSKGL VERFQAFERM LANAPGWQGR VSLVQIAPPT RSDVQTYQRI RETLEGEAGR INGRFSQLDW TPIQYLNRKY ERNLLMAFFR MSQVGYVTPL RDGMNLVAKE YVASQDPADP GVLVLSEFAG AAAELTGALL VNPYDLSQMA DALERALSMP LAERQARHEE NLARLRANDL SVWRDTFVAD LRSVAAAASV TQRAGRRIAH A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA GATTGATCGT GGTATCGAAC CGTGTCGCCA TCGGCGAGGA CACACGCCCG AGCGCGGGCG GCCTCGCGGT CGGCGTGATG GACGCGCTGC AGGAAACGGG CGGCGTCTGG TTCGGCTGGA ACGGCGAGAT CGTGGGGACG CCGGACGCGG CGCCGGCGAT CCGGCGCGAC GGCAACGTGA CTTATGCGAC GGTCGGACTG ACGCGCCGCG ACTACGATCA GTACTATCGC GGGTTTTCGA ACGCGACGCT GTGGCCCGTG TTCCACTATC GCGGCGATCT CGCGCGCTTC GACCGACAGG AGTACGCCGG CTATCTGCGC GTGAACGCGA TGCTCGCGAA GCAGCTCGCC GCACTGCTGC GCCCGGACGA TCTGATCTGG GTGCACGACT ACCATCTGCT GCCGTTCGCG CACGCGCTGC GCGAGCTCGG CGTGAAGAAT CCGATCGGCT TCTTCCTGCA CATTCCGTTT CCGTCGCCGG ACGTGCTGCG GCTCGTGCCG CCGCACGACG AACTCGTGAA GTTCATGTGC GCGTATGACG TGACGGGCTT CCAGACCGAT GCCGACAGGC AGGCGTTCAC CGACTACATC GAGCGGCGCG GGATCGGCAC CGCAAGCGAG GACGGCATGC TGCACGCACA CGGCCGCGTC GTGAAGGTGG CCGCGTATCC GATCGGCGTG TATCCGGATG CGATCGCGCA GGCGGCCGTC CAGTACGGCG CGCGCAAGCC GGTGAAGATG CTGCGCGACG CACTGGGCGG CCGCAAGCTC GTGATGAGCG TCGATCGTCT CGACTATTCG AAGGGGCTCG TCGAACGCTT CCAGGCGTTC GAGCGCATGC TCGCGAATGC GCCGGGCTGG CAGGGGCGCG TGTCGCTCGT GCAGATCGCG CCGCCGACGC GTTCCGACGT GCAGACGTAC CAGCGAATCC GCGAAACGCT CGAAGGCGAG GCCGGCCGCA TCAACGGACG GTTTTCGCAG CTCGACTGGA CGCCGATTCA ATATCTGAAC CGCAAGTACG AGCGCAATCT GCTGATGGCG TTCTTCCGCA TGTCGCAGGT CGGCTACGTG ACGCCGCTGC GCGACGGGAT GAACCTCGTC GCGAAGGAAT ACGTCGCGTC GCAGGATCCG GCCGATCCGG GCGTGCTCGT GCTGTCCGAA TTTGCGGGCG CCGCGGCGGA GCTGACGGGC GCGCTGCTCG TCAATCCGTA TGACCTCTCG CAGATGGCCG ACGCGCTCGA GCGTGCACTT TCGATGCCGC TCGCGGAACG GCAGGCGCGT CACGAGGAGA ACCTCGCGCG GCTGCGCGCG AACGACCTGT CGGTGTGGCG CGATACGTTC GTCGCCGATC TGCGCAGCGT CGCGGCGGCG GCCTCGGTCA CGCAGCGCGC GGGCCGACGC ATCGCGCATG CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for BumuA.00046.b.B11.PS37890
| PURIFICATION DATe: | 3/29/2016 |
| CONCENTRATION: | 51.2mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS RLIVVSNRVA IGEDTRPSAG GLAVGVMDAL QETGGVWFGW NGEIVGTPDA APAIRRDGNV TYATVGLTRR DYDQYYRGFS NATLWPVFHY RGDLARFDRQ EYAGYLRVNA MLAKQLAALL RPDDLIWVHD YHLLPFAHAL RELGVKNPIG FFLHIPFPSP DVLRLVPPHD ELVKFMCAYD VTGFQTDADR QAFTDYIERR GIGTASEDGM LHAHGRVVKV AAYPIGVYPD AIAQAAVQYG ARKPVKMLRD ALGGRKLVMS VDRLDYSKGL VERFQAFERM LANAPGWQGR VSLVQIAPPT RSDVQTYQRI RETLEGEAGR INGRFSQLDW TPIQYLNRKY ERNLLMAFFR MSQVGYVTPL RDGMNLVAKE YVASQDPADP GVLVLSEFAG AAAELTGALL VNPYDLSQMA DALERALSMP LAERQARHEE NLARLRANDL SVWRDTFVAD LRSVAAAASV TQRAGRRIAH A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA GATTGATCGT GGTATCGAAC CGTGTCGCCA TCGGCGAGGA CACACGCCCG AGCGCGGGCG GCCTCGCGGT CGGCGTGATG GACGCGCTGC AGGAAACGGG CGGCGTCTGG TTCGGCTGGA ACGGCGAGAT CGTGGGGACG CCGGACGCGG CGCCGGCGAT CCGGCGCGAC GGCAACGTGA CTTATGCGAC GGTCGGACTG ACGCGCCGCG ACTACGATCA GTACTATCGC GGGTTTTCGA ACGCGACGCT GTGGCCCGTG TTCCACTATC GCGGCGATCT CGCGCGCTTC GACCGACAGG AGTACGCCGG CTATCTGCGC GTGAACGCGA TGCTCGCGAA GCAGCTCGCC GCACTGCTGC GCCCGGACGA TCTGATCTGG GTGCACGACT ACCATCTGCT GCCGTTCGCG CACGCGCTGC GCGAGCTCGG CGTGAAGAAT CCGATCGGCT TCTTCCTGCA CATTCCGTTT CCGTCGCCGG ACGTGCTGCG GCTCGTGCCG CCGCACGACG AACTCGTGAA GTTCATGTGC GCGTATGACG TGACGGGCTT CCAGACCGAT GCCGACAGGC AGGCGTTCAC CGACTACATC GAGCGGCGCG GGATCGGCAC CGCAAGCGAG GACGGCATGC TGCACGCACA CGGCCGCGTC GTGAAGGTGG CCGCGTATCC GATCGGCGTG TATCCGGATG CGATCGCGCA GGCGGCCGTC CAGTACGGCG CGCGCAAGCC GGTGAAGATG CTGCGCGACG CACTGGGCGG CCGCAAGCTC GTGATGAGCG TCGATCGTCT CGACTATTCG AAGGGGCTCG TCGAACGCTT CCAGGCGTTC GAGCGCATGC TCGCGAATGC GCCGGGCTGG CAGGGGCGCG TGTCGCTCGT GCAGATCGCG CCGCCGACGC GTTCCGACGT GCAGACGTAC CAGCGAATCC GCGAAACGCT CGAAGGCGAG GCCGGCCGCA TCAACGGACG GTTTTCGCAG CTCGACTGGA CGCCGATTCA ATATCTGAAC CGCAAGTACG AGCGCAATCT GCTGATGGCG TTCTTCCGCA TGTCGCAGGT CGGCTACGTG ACGCCGCTGC GCGACGGGAT GAACCTCGTC GCGAAGGAAT ACGTCGCGTC GCAGGATCCG GCCGATCCGG GCGTGCTCGT GCTGTCCGAA TTTGCGGGCG CCGCGGCGGA GCTGACGGGC GCGCTGCTCG TCAATCCGTA TGACCTCTCG CAGATGGCCG ACGCGCTCGA GCGTGCACTT TCGATGCCGC TCGCGGAACG GCAGGCGCGT CACGAGGAGA ACCTCGCGCG GCTGCGCGCG AACGACCTGT CGGTGTGGCG CGATACGTTC GTCGCCGATC TGCGCAGCGT CGCGGCGGCG GCCTCGGTCA CGCAGCGCGC GGGCCGACGC ATCGCGCATG CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT