BumuA.00039.a

Methionine aminopeptidase

CENTER ID: BumuA.00039.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00039.a.B1.GE39664 Full length( BumuA.00039.a ) 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00039.a.B1.PW37877 Full length( BumuA.00039.a ) 1 268

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.2376
RefSeq: YP_001580338.1
UniProt: A0A0H3KI71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKREIPIRN PQDIAKSREA AKLASQVLTM IGEYVKPGVT TDELDERCRR YIVDELGAIP ANIGYHGYPK TVCASVNHVV CHGIPSSRPL RDGDIVNLDI AVIKDGWFGD TSRMYFVGEP GELARRLVAT TYEAMHAGIR AVRPGATLGD VGYAIQQVAH REGFSVVREY CGHGIGDVYH DEPQVLHYGR PGTGLPLRPG MIFTIEPMLN AGKRDTRVLG DGWTVVTKDH SLSAQWEHMV VVTEQGYEVL TDDVGPPAFA ELPGTRVA
NT Sequence
ATGGCGAAGC GCGAAATCCC GATCCGCAAC CCGCAAGACA TCGCCAAGTC GCGCGAGGCG GCGAAGCTCG CGTCGCAGGT GCTGACGATG ATCGGCGAAT ATGTGAAGCC GGGCGTCACG ACCGACGAAC TGGACGAACG CTGCCGCCGC TATATCGTCG ACGAACTCGG CGCGATTCCG GCCAACATCG GCTATCACGG CTATCCGAAG ACGGTCTGCG CGTCGGTCAA TCACGTCGTC TGCCACGGCA TTCCGTCGTC GCGGCCGCTG CGCGACGGCG ACATCGTGAA CCTCGACATC GCGGTGATCA AGGACGGCTG GTTCGGCGAC ACGAGCCGCA TGTACTTCGT CGGCGAGCCG GGCGAGCTGG CGCGCCGCCT CGTCGCGACG ACGTACGAGG CGATGCATGC GGGCATCCGC GCGGTGCGGC CGGGCGCGAC GCTCGGCGAC GTCGGCTATG CGATCCAGCA GGTCGCACAT CGCGAAGGGT TCAGCGTCGT GCGCGAATAC TGCGGGCACG GCATCGGCGA CGTGTATCAC GACGAGCCGC AAGTGCTGCA CTACGGCCGC CCCGGCACAG GGCTGCCGCT GCGGCCGGGC ATGATCTTCA CGATCGAGCC GATGCTCAAC GCAGGCAAGC GCGACACGCG CGTGCTCGGC GACGGCTGGA CCGTCGTCAC GAAGGACCAT TCGCTGTCCG CCCAATGGGA GCACATGGTG GTCGTGACGG AGCAGGGCTA CGAAGTGCTG ACCGACGACG TGGGGCCGCC CGCGTTCGCC GAACTGCCCG GCACGCGCGT CGCC
Details for BumuA.00039.a.B1.GE39664
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA KREIPIRNPQ DIAKSREAAK LASQVLTMIG EYVKPGVTTD ELDERCRRYI VDELGAIPAN IGYHGYPKTV CASVNHVVCH GIPSSRPLRD GDIVNLDIAV IKDGWFGDTS RMYFVGEPGE LARRLVATTY EAMHAGIRAV RPGATLGDVG YAIQQVAHRE GFSVVREYCG HGIGDVYHDE PQVLHYGRPG TGLPLRPGMI FTIEPMLNAG KRDTRVLGDG WTVVTKDHSL SAQWEHMVVV TEQGXXVLTD XXGPPAFAXX XGTRVXXXSK
Validated NT Sequence
atggctcacc accaccacca ccatatggcg aagcgcgaaa tcccgatccg caacccgcaa gacatcgcca agtcgcgcga ggcggcgaag ctcgcgtcgc aggtgctgac gatgatcggc gaatatgtga agccgggcgt cacgaccgac gaactggacg aacgctgccg ccgctatatc gtcgacgaac tcggcgcgat tccggccaac atcggctatc acggctatcc gaagacggtc tgcgcgtcgg tcaatcacgt cgtctgccac ggcattccgt cgtcgcggcc gctgcgcgac ggcgacatcg tgaacctcga catcgcggtg atcaaggacg gctggttcgg cgacacgagc cgcatgtact tcgtcggcga gccgggcgag ctggcgcgcc gcctcgtcgc gacgacgtac gaggcgatgc atgcgggcat ccgcgcggtg cggccgggcg cgacgctcgg cgacgtcggc tatgcgatcc agcaggtcgc acatcgcgaa gggttcagcg tcgtgcgcga atactgcggg cacggcatcg gcgacgtgta tcacgacgag ccgcaagtgc tgcactacgg ccgccccggc acagggctgc cgctgcggcc gggcatgatc ttcacgatcg agccgatgct caacgcaggc aagcgcgaca cgcgcgtgct cggcgacggc tggaccgtcg tcacgaagga ccattcgctg tccgcccaat gggagcacat ggtggtcgtg acggagcagg gctacgaagt gctgaccgac gacgtggggc cgcccgcgtt cgccgaactg cccggcacgc gcgtcgccta a
Expected Protein Sequence
MAHHHHHHMA KREIPIRNPQ DIAKSREAAK LASQVLTMIG EYVKPGVTTD ELDERCRRYI VDELGAIPAN IGYHGYPKTV CASVNHVVCH GIPSSRPLRD GDIVNLDIAV IKDGWFGDTS RMYFVGEPGE LARRLVATTY EAMHAGIRAV RPGATLGDVG YAIQQVAHRE GFSVVREYCG HGIGDVYHDE PQVLHYGRPG TGLPLRPGMI FTIEPMLNAG KRDTRVLGDG WTVVTKDHSL SAQWEHMVVV TEQGYEVLTD DVGPPAFAEL PGTRVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga agcgcgaaat cccgatccgc aacccgcaag acatcgccaa gtcgcgcgag gcggcgaagc tcgcgtcgca ggtgctgacg atgatcggcg aatatgtgaa gccgggcgtc acgaccgacg aactggacga acgctgccgc cgctatatcg tcgacgaact cggcgcgatt ccggccaaca tcggctatca cggctatccg aagacggtct gcgcgtcggt caatcacgtc gtctgccacg gcattccgtc gtcgcggccg ctgcgcgacg gcgacatcgt gaacctcgac atcgcggtga tcaaggacgg ctggttcggc gacacgagcc gcatgtactt cgtcggcgag ccgggcgagc tggcgcgccg cctcgtcgcg acgacgtacg aggcgatgca tgcgggcatc cgcgcggtgc ggccgggcgc gacgctcggc gacgtcggct atgcgatcca gcaggtcgca catcgcgaag ggttcagcgt cgtgcgcgaa tactgcgggc acggcatcgg cgacgtgtat cacgacgagc cgcaagtgct gcactacggc cgccccggca cagggctgcc gctgcggccg ggcatgatct tcacgatcga gccgatgctc aacgcaggca agcgcgacac gcgcgtgctc ggcgacggct ggaccgtcgt cacgaaggac cattcgctgt ccgcccaatg ggagcacatg gtggtcgtga cggagcaggg ctacgaagtg ctgaccgacg acgtggggcc gcccgcgttc gccgaactgc ccggcacgcg cgtcgcctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BumuA.00039.a.B1.PW37877
PURIFICATION DATe: 3/22/2016
CONCENTRATION: 31mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KREIPIRNPQ DIAKSREAAK LASQVLTMIG EYVKPGVTTD ELDERCRRYI VDELGAIPAN IGYHGYPKTV CASVNHVVCH GIPSSRPLRD GDIVNLDIAV IKDGWFGDTS RMYFVGEPGE LARRLVATTY EAMHAGIRAV RPGATLGDVG YAIQQVAHRE GFSVVREYCG HGIGDVYHDE PQVLHYGRPG TGLPLRPGMI FTIEPMLNAG KRDTRVLGDG WTVVTKDHSL SAQWEHMVVV TEQGXXVLTD XXGPPAFAXX XGTRVXXXSK
Validated NT Sequence
atggctcacc accaccacca ccatatggcg aagcgcgaaa tcccgatccg caacccgcaa gacatcgcca agtcgcgcga ggcggcgaag ctcgcgtcgc aggtgctgac gatgatcggc gaatatgtga agccgggcgt cacgaccgac gaactggacg aacgctgccg ccgctatatc gtcgacgaac tcggcgcgat tccggccaac atcggctatc acggctatcc gaagacggtc tgcgcgtcgg tcaatcacgt cgtctgccac ggcattccgt cgtcgcggcc gctgcgcgac ggcgacatcg tgaacctcga catcgcggtg atcaaggacg gctggttcgg cgacacgagc cgcatgtact tcgtcggcga gccgggcgag ctggcgcgcc gcctcgtcgc gacgacgtac gaggcgatgc atgcgggcat ccgcgcggtg cggccgggcg cgacgctcgg cgacgtcggc tatgcgatcc agcaggtcgc acatcgcgaa gggttcagcg tcgtgcgcga atactgcggg cacggcatcg gcgacgtgta tcacgacgag ccgcaagtgc tgcactacgg ccgccccggc acagggctgc cgctgcggcc gggcatgatc ttcacgatcg agccgatgct caacgcaggc aagcgcgaca cgcgcgtgct cggcgacggc tggaccgtcg tcacgaagga ccattcgctg tccgcccaat gggagcacat ggtggtcgtg acggagcagg gctacgaagt gctgaccgac gacgtggggc cgcccgcgtt cgccgaactg cccggcacgc gcgtcgccta a
Expressed Protein Sequence
MAHHHHHHMA KREIPIRNPQ DIAKSREAAK LASQVLTMIG EYVKPGVTTD ELDERCRRYI VDELGAIPAN IGYHGYPKTV CASVNHVVCH GIPSSRPLRD GDIVNLDIAV IKDGWFGDTS RMYFVGEPGE LARRLVATTY EAMHAGIRAV RPGATLGDVG YAIQQVAHRE GFSVVREYCG HGIGDVYHDE PQVLHYGRPG TGLPLRPGMI FTIEPMLNAG KRDTRVLGDG WTVVTKDHSL SAQWEHMVVV TEQGYEVLTD DVGPPAFAEL PGTRVA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCGA AGCGCGAAAT CCCGATCCGC AACCCGCAAG ACATCGCCAA GTCGCGCGAG GCGGCGAAGC TCGCGTCGCA GGTGCTGACG ATGATCGGCG AATATGTGAA GCCGGGCGTC ACGACCGACG AACTGGACGA ACGCTGCCGC CGCTATATCG TCGACGAACT CGGCGCGATT CCGGCCAACA TCGGCTATCA CGGCTATCCG AAGACGGTCT GCGCGTCGGT CAATCACGTC GTCTGCCACG GCATTCCGTC GTCGCGGCCG CTGCGCGACG GCGACATCGT GAACCTCGAC ATCGCGGTGA TCAAGGACGG CTGGTTCGGC GACACGAGCC GCATGTACTT CGTCGGCGAG CCGGGCGAGC TGGCGCGCCG CCTCGTCGCG ACGACGTACG AGGCGATGCA TGCGGGCATC CGCGCGGTGC GGCCGGGCGC GACGCTCGGC GACGTCGGCT ATGCGATCCA GCAGGTCGCA CATCGCGAAG GGTTCAGCGT CGTGCGCGAA TACTGCGGGC ACGGCATCGG CGACGTGTAT CACGACGAGC CGCAAGTGCT GCACTACGGC CGCCCCGGCA CAGGGCTGCC GCTGCGGCCG GGCATGATCT TCACGATCGA GCCGATGCTC AACGCAGGCA AGCGCGACAC GCGCGTGCTC GGCGACGGCT GGACCGTCGT CACGAAGGAC CATTCGCTGT CCGCCCAATG GGAGCACATG GTGGTCGTGA CGGAGCAGGG CTACGAAGTG CTGACCGACG ACGTGGGGCC GCCCGCGTTC GCCGAACTGC CCGGCACGCG CGTCGCCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT