SSGCID: Target Page

BumuA.00036.a

Phosphoribosylaminoimidazole carboxylase ATPase subunit

CENTER ID:	BumuA.00036.a
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00036.a.A1.GE33072	Full length( BumuA.00036.a )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00036.a.A1.PS01262	Full length( BumuA.00036.a )		1	398

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.821
RefSeq:	YP_001578867.1
UniProt:	A0A0H3KLT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPASP AGAVADRHLR AAYDDEAALT ELAALCDAVS TEFENVPAAS LDFLARTTFV APAGRCVAIA QDRIAEKRFI EASGVPVAPH VAIESADALA ALDDAALDAV LPGILKTARL GYDGKGQVRV RTAQEAREAY ASLGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN AHHNGILALT VVPAPAADDA LVAQAQQAAV RIADTLGYVG VLCVEFFVLE DGSLVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPQGAESDAV TPPWDAVAAM PTAHLHLYGK EEARVGRKMG HVNFTAATRD EAVAAAHACA QLLRVPLD

NT Sequence

ATGACCGCAA CTCCTGATTC CGTTTCCCCG ATCCTGCCCG GCGCGTGGCT GGGCATGGTC GGCGGCGGCC AGCTCGGCCG CATGTTCTGT TTTGCCGCCC AGTCGATGGG CTATCGCGTC GCCGTGCTCG ATCCCGATCC GGCGAGCCCC GCCGGCGCGG TCGCCGATCG CCACCTGCGC GCCGCGTATG ACGACGAAGC CGCGCTGACC GAACTGGCCG CGCTGTGCGA CGCGGTATCG ACCGAATTCG AGAACGTGCC GGCCGCGAGC CTCGATTTCC TCGCGCGCAC GACGTTCGTC GCGCCGGCCG GCCGCTGCGT CGCGATCGCG CAGGACCGGA TCGCCGAAAA GCGCTTCATC GAGGCGTCGG GCGTACCGGT CGCGCCGCAC GTCGCGATCG AATCGGCCGA CGCGCTCGCG GCGCTCGACG ACGCAGCGCT CGACGCGGTG CTGCCGGGCA TTCTGAAGAC CGCGCGGCTC GGCTACGACG GCAAGGGCCA GGTGCGTGTG CGCACCGCGC AGGAAGCGCG CGAGGCGTAT GCGTCGCTCG GCGGCGTGCC GTGCGTGCTC GAAAAGCGCC TGCCGCTGAA ATACGAGGTG TCCGCACTGA TCGCGCGCGG CGCCGACGGC CGCTCGGCCG CGTTCCCGCT CGCGCAGAAC GCGCACCACA ACGGCATTCT CGCGCTGACC GTCGTGCCGG CGCCGGCCGC CGACGACGCG CTCGTCGCGC AGGCGCAGCA GGCCGCCGTG CGCATCGCCG ATACGCTCGG CTACGTCGGC GTGCTGTGCG TCGAGTTCTT CGTGCTCGAA GACGGTTCGC TCGTGGCGAA CGAGATGGCG CCGCGCCCGC ACAATTCCGG CCACTACACG GTCGATGCGT GCGCAACGAG CCAGTTCGAG CAGCAGGTGC GCGCAATGAC GCGCATGCCG CTCGGCAATC CGCGCCAGCA TTCGCCGGCC GCGATGCTGA ACATTCTCGG CGACGTCTGG TTTCCGCAAG GCGCGGAAAG CGACGCCGTC ACGCCGCCGT GGGACGCGGT TGCCGCGATG CCGACCGCGC ATCTGCATCT GTACGGCAAG GAAGAGGCGC GCGTCGGCCG CAAGATGGGC CACGTGAATT TCACGGCCGC GACGCGCGAC GAAGCGGTCG CCGCCGCGCA CGCGTGCGCG CAGCTGCTGC GCGTGCCGCT CGAC

Details for BumuA.00036.a.A1.GE33072

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattagtcg agcggcacgc gcagcagctg cgcgcacgcg tgcgcggcgg cgaccgcttc gtcgcgcgtc gcggccgtga aattcacgtg gcccatcttg cggccgacgc gcgcctcttc cttgccgtac agatgcagat gcgcggtcgg catcgcggca accgcgtccc acggcggcgt gacggcgtcg ctttccgcgc cttgcggaaa ccagacgtcg ccgagaatgt tcagcatcgc ggccggcgaa tgctggcgcg gattgccgag cggcatgcgc gtcattgcgc gcacctgctg ctcgaactgg ctcgttgcgc acgcatcgac cgtgtagtgg ccggaattgt gcgggcgcgg cgccatctcg ttcgccacga gcgaaccgtc ttcgagcacg aagaactcga cgcacagcac gccgacgtag ccgagcgtat cggcgatgcg cacggcggcc tgctgcgcct gcgcgacgag cgcgtcgtcg gcggccggcg ccggcacgac ggtcagcgcg agaatgccgt tgtggtgcgc gttctgcgcg agcgggaacg cggccgagcg gccgtcggcg ccgcgcgcga tcagtgcgga cacctcgtat ttcagcggca ggcgcttttc gagcacgcac ggcacgccgc cgagcgacgc atacgcctcg cgcgcttcct gcgcggtgcg cacacgcacc tggcccttgc cgtcgtagcc gagccgcgcg gtcttcagaa tgcccggcag caccgcgtcg agcgctgcgt cgtcgagcgc cgcgagcgcg tcggccgatt cgatcgcgac gtgcggcgcg accggtacgc ccgacgcctc gatgaagcgc ttttcggcga tccggtcctg cgcgatcgcg acgcagcggc cggccggcgc gacgaacgtc gtgcgcgcga ggaaatcgag gctcgcggcc ggcacgttct cgaattcggt cgataccgcg tcgcacagcg cggccagttc ggtcagcgcg gcttcgtcgt catacgcggc gcgcaggtgg cgatcggcga ccgcgccggc ggggctcgcc ggatcgggat cgagcacggc gacgcgatag cccatcgact gggcggcaaa acagaacatg cggccgagct ggccgccgcc gaccatgccc agccacgcgc cgggcaggat cggggaaacg gaatcaggag ttgcggtcat cgaaccagga ccctgggtct gagcttccag gg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTATPDSVS PILPGAWLGM VGGGQLGRMF CFAAQSMGYR VAVLDPDPAS PAGAVADRHL RAAYDDEAAL TELAALCDAV STEFENVPAA SLDFLARTTF VAPAGRCVAI AQDRIAEKRF IEASGVPVAP HVAIESADAL AALDDAALDA VLPGILKTAR LGYDGKGQVR VRTAQEAREA YASLGGVPCV LEKRLPLKYE VSALIARGAD GRSAAFPLAQ NAHHNGILAL TVVPAPAADD ALVAQAQQAA VRIADTLGYV GVLCVEFFVL EDGSLVANEM APRPHNSGHY TVDACATSQF EQQVRAMTRM PLGNPRQHSP AAMLNILGDV WFPQGAESDA VTPPWDAVAA MPTAHLHLYG KEEARVGRKM GHVNFTAATR DEAVAAAHAC AQLLRVPLD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcaact cctgattccg tttccccgat cctgcccggc gcgtggctgg gcatggtcgg cggcggccag ctcggccgca tgttctgttt tgccgcccag tcgatgggct atcgcgtcgc cgtgctcgat cccgatccgg cgagccccgc cggcgcggtc gccgatcgcc acctgcgcgc cgcgtatgac gacgaagccg cgctgaccga actggccgcg ctgtgcgacg cggtatcgac cgaattcgag aacgtgccgg ccgcgagcct cgatttcctc gcgcgcacga cgttcgtcgc gccggccggc cgctgcgtcg cgatcgcgca ggaccggatc gccgaaaagc gcttcatcga ggcgtcgggc gtaccggtcg cgccgcacgt cgcgatcgaa tcggccgacg cgctcgcggc gctcgacgac gcagcgctcg acgcggtgct gccgggcatt ctgaagaccg cgcggctcgg ctacgacggc aagggccagg tgcgtgtgcg caccgcgcag gaagcgcgcg aggcgtatgc gtcgctcggc ggcgtgccgt gcgtgctcga aaagcgcctg ccgctgaaat acgaggtgtc cgcactgatc gcgcgcggcg ccgacggccg ctcggccgcg ttcccgctcg cgcagaacgc gcaccacaac ggcattctcg cgctgaccgt cgtgccggcg ccggccgccg acgacgcgct cgtcgcgcag gcgcagcagg ccgccgtgcg catcgccgat acgctcggct acgtcggcgt gctgtgcgtc gagttcttcg tgctcgaaga cggttcgctc gtggcgaacg agatggcgcc gcgcccgcac aattccggcc actacacggt cgatgcgtgc gcaacgagcc agttcgagca gcaggtgcgc gcaatgacgc gcatgccgct cggcaatccg cgccagcatt cgccggccgc gatgctgaac attctcggcg acgtctggtt tccgcaaggc gcggaaagcg acgccgtcac gccgccgtgg gacgcggttg ccgcgatgcc gaccgcgcat ctgcatctgt acggcaagga agaggcgcgc gtcggccgca agatgggcca cgtgaatttc acggccgcga cgcgcgacga agcggtcgcc gccgcgcacg cgtgcgcgca gctgctgcgc gtgccgctcg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BumuA.00036.a.A1.PS01262

PURIFICATION DATe:	11/17/2011
CONCENTRATION:	70.4mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCAACT CCTGATTCCG TTTCCCCGAT CCTGCCCGGC GCGTGGCTGG GCATGGTCGG CGGCGGCCAG CTCGGCCGCA TGTTCTGTTT TGCCGCCCAG TCGATGGGCT ATCGCGTCGC CGTGCTCGAT CCCGATCCGG CGAGCCCCGC CGGCGCGGTC GCCGATCGCC ACCTGCGCGC CGCGTATGAC GACGAAGCCG CGCTGACCGA ACTGGCCGCG CTGTGCGACG CGGTATCGAC CGAATTCGAG AACGTGCCGG CCGCGAGCCT CGATTTCCTC GCGCGCACGA CGTTCGTCGC GCCGGCCGGC CGCTGCGTCG CGATCGCGCA GGACCGGATC GCCGAAAAGC GCTTCATCGA GGCGTCGGGC GTACCGGTCG CGCCGCACGT CGCGATCGAA TCGGCCGACG CGCTCGCGGC GCTCGACGAC GCAGCGCTCG ACGCGGTGCT GCCGGGCATT CTGAAGACCG CGCGGCTCGG CTACGACGGC AAGGGCCAGG TGCGTGTGCG CACCGCGCAG GAAGCGCGCG AGGCGTATGC GTCGCTCGGC GGCGTGCCGT GCGTGCTCGA AAAGCGCCTG CCGCTGAAAT ACGAGGTGTC CGCACTGATC GCGCGCGGCG CCGACGGCCG CTCGGCCGCG TTCCCGCTCG CGCAGAACGC GCACCACAAC GGCATTCTCG CGCTGACCGT CGTGCCGGCG CCGGCCGCCG ACGACGCGCT CGTCGCGCAG GCGCAGCAGG CCGCCGTGCG CATCGCCGAT ACGCTCGGCT ACGTCGGCGT GCTGTGCGTC GAGTTCTTCG TGCTCGAAGA CGGTTCGCTC GTGGCGAACG AGATGGCGCC GCGCCCGCAC AATTCCGGCC ACTACACGGT CGATGCGTGC GCAACGAGCC AGTTCGAGCA GCAGGTGCGC GCAATGACGC GCATGCCGCT CGGCAATCCG CGCCAGCATT CGCCGGCCGC GATGCTGAAC ATTCTCGGCG ACGTCTGGTT TCCGCAAGGC GCGGAAAGCG ACGCCGTCAC GCCGCCGTGG GACGCGGTTG CCGCGATGCC GACCGCGCAT CTGCATCTGT ACGGCAAGGA AGAGGCGCGC GTCGGCCGCA AGATGGGCCA CGTGAATTTC ACGGCCGCGA CGCGCGACGA AGCGGTCGCC GCCGCGCACG CGTGCGCGCA GCTGCTGCGC GTGCCGCTCG ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA