BumuA.00035.a

Ribose-phosphate pyrophosphokinase

CENTER ID: BumuA.00035.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00035.a.B1.GE39663 Full length( BumuA.00035.a ) 1 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.6235
RefSeq: YP_001585877.1
UniProt: A0A0H3KQR4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRGSHATER LPFSLFAFAS CRGVGERIAA RLGVTLAAHE ERPFEDGEHK TRALASVRGH DVYVVQSLHG EPGISVNDKL CRLLLFIGAL KDASAARVCA VTPYLCYSRK DRRSHPRDPV STRYVGTLLE AVGVDRVVAI DIHNIAAFQN AFRCPTEHLE ANGMLVEWLA SQAGERPVVV VSPDAGGARR AEDFRARLEA RIGRPVDAAF AGELRSGGIV TPKPIVGEVA GRCAVIVDDL IGTGTTLART AENCRTLGAQ SVYAVATHGM FFDDAPVLLG GDALAGIAVT DTVPPWRVPA GPLKDKLAVL DSAPLFAEAI ARIHRGGSLT DLLRR
NT Sequence
ATGAATCGAG GTAGCCATGC CACGGAACGA CTCCCTTTCA GCCTGTTTGC CTTCGCCTCG TGTCGCGGGG TCGGCGAACG AATCGCAGCC CGTCTCGGCG TCACGCTCGC CGCGCACGAG GAACGTCCGT TCGAGGACGG TGAACACAAA ACGCGCGCGC TTGCCAGCGT GCGCGGACAC GACGTCTACG TCGTTCAGTC GCTGCACGGA GAGCCAGGCA TTTCCGTCAA CGACAAGCTG TGCCGGTTGC TGCTGTTCAT CGGTGCGTTG AAGGACGCGT CGGCCGCACG CGTCTGTGCG GTTACACCCT ACCTGTGCTA TTCGCGCAAG GACCGCCGCT CTCATCCACG CGATCCGGTA TCCACGCGCT ATGTCGGCAC GTTGCTCGAA GCGGTGGGTG TCGATCGTGT CGTCGCGATC GATATTCACA ACATCGCGGC GTTCCAGAAC GCGTTTCGCT GCCCGACGGA GCATCTCGAG GCGAACGGCA TGCTGGTCGA ATGGCTCGCC TCGCAAGCCG GCGAACGGCC GGTCGTGGTC GTATCGCCCG ACGCAGGCGG CGCGCGGCGG GCCGAGGATT TCCGTGCGAG GCTGGAAGCG CGCATCGGGC GTCCGGTCGA CGCGGCATTC GCCGGCGAGC TGCGCAGCGG AGGCATCGTG ACGCCCAAGC CGATCGTCGG CGAGGTCGCC GGCCGTTGCG CCGTCATCGT CGACGACCTG ATCGGTACCG GGACGACATT GGCCCGCACG GCCGAGAATT GCCGCACGCT CGGCGCACAA TCCGTCTACG CGGTCGCGAC GCATGGGATG TTCTTTGATG ACGCGCCCGT ACTGCTCGGC GGCGATGCGC TCGCCGGCAT CGCCGTGACC GACACGGTGC CGCCCTGGCG CGTGCCGGCC GGGCCGCTCA AGGACAAGCT CGCGGTGCTC GACAGCGCTC CGCTGTTTGC CGAGGCGATT GCGCGTATCC ATCGCGGCGG CTCGCTCACC GACCTGTTGC GGCGC
Details for BumuA.00035.a.B1.GE39663
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 87
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHXXX XXXXXTERXP XXXXXXXXXX GXXEXXXAXX GXTLAAHEEX XXEXGXHKTR AXXSVRGHDX XXVQXXHGEP GISVNDKLCR XLLFIGALKD ASAARVCAVT PYLCYSRKDR RSHPRDPVST XXVGTLLEAV GVDRVVAIDI HNIAAFQNAF RCPTEHLEAN GMLVEWLASQ AGERPVVVVS PDAGGARRAE DFRARLEARI GRPVDAAFAG ELRSGGIVTP KPIVGEVAGR CAVIVDDLIG TGTTLARTAE NCRTLGAQSV YAVATHGMFF DDAPVLLGGD ALAGIAVTDT VPPWRVPAGP LKDKLAVLDS APLFAEAIAR IH
Validated NT Sequence
cgatggatac gcgcaatcgc ctcggcaaac agcggagcgc tgtcgagcac cgcgagcttg tccttgagcg gcccggccgg cacgcgccag ggcggcaccg tgtcggtcac ggcgatgccg gcgagcgcat cgccgccgag cagtacgggc gcgtcatcaa agaacatccc atgcgtcgcg accgcgtaga cggattgtgc gccgagcgtg cggcaattct cggccgtgcg ggccaatgtc gtcccggtac cgatcaggtc gtcgacgatg acggcgcaac ggccggcgac ctcgccgacg atcggcttgg gcgtcacgat gcctccgctg cgcagctcgc cggcgaatgc cgcgtcgacc ggacgcccga tgcgcgcttc cagcctcgca cggaaatcct cggcccgccg cgcgccgcct gcgtcgggcg atacgaccac gaccggccgt tcgccggctt gcgaggcgag ccattcgacc agcatgccgt tcgcctcgag atgctccgtc gggcagcgaa acgcgttctg gaacgccgcg atgttgtgaa tatcgatcgc gacgacacga tcgacaccca ccgcttcgag caacgtgccg acatnnngcg tggataccgg atcgcgtgga tgagagcggc ggtccttgcg cgaatagcac aggtagggtg taaccgcaca gacgcgtgcg gccgacgcgt ccttcaacgc accgatgaac agcagcannc ggcacagctt gtcgttgacg gaaatgcctg gctctccgtg cngcnnctga acgnngnnnn ngtcgtgtcc gcgcacgctg nnnnncgcgc gcgttttgtg tnnnccntcc tcnnncgnnn nttcctcgtg cgcggcnagc gtnnngccnn nnngggctgn nnnnngttcg nnnnncccgc nnnnnnnnnn nnnnnnnnnn nnnntnnnag gnnntcgttc cgtggnnngg cnnnnnnnnn tnnnnnggtg gtggtggtgg tgagccatg
Expected Protein Sequence
MAHHHHHHMN RGSHATERLP FSLFAFASCR GVGERIAARL GVTLAAHEER PFEDGEHKTR ALASVRGHDV YVVQSLHGEP GISVNDKLCR LLLFIGALKD ASAARVCAVT PYLCYSRKDR RSHPRDPVST RYVGTLLEAV GVDRVVAIDI HNIAAFQNAF RCPTEHLEAN GMLVEWLASQ AGERPVVVVS PDAGGARRAE DFRARLEARI GRPVDAAFAG ELRSGGIVTP KPIVGEVAGR CAVIVDDLIG TGTTLARTAE NCRTLGAQSV YAVATHGMFF DDAPVLLGGD ALAGIAVTDT VPPWRVPAGP LKDKLAVLDS APLFAEAIAR IHRGGSLTDL LRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc gaggtagcca tgccacggaa cgactccctt tcagcctgtt tgccttcgcc tcgtgtcgcg gggtcggcga acgaatcgca gcccgtctcg gcgtcacgct cgccgcgcac gaggaacgtc cgttcgagga cggtgaacac aaaacgcgcg cgcttgccag cgtgcgcgga cacgacgtct acgtcgttca gtcgctgcac ggagagccag gcatttccgt caacgacaag ctgtgccggt tgctgctgtt catcggtgcg ttgaaggacg cgtcggccgc acgcgtctgt gcggttacac cctacctgtg ctattcgcgc aaggaccgcc gctctcatcc acgcgatccg gtatccacgc gctatgtcgg cacgttgctc gaagcggtgg gtgtcgatcg tgtcgtcgcg atcgatattc acaacatcgc ggcgttccag aacgcgtttc gctgcccgac ggagcatctc gaggcgaacg gcatgctggt cgaatggctc gcctcgcaag ccggcgaacg gccggtcgtg gtcgtatcgc ccgacgcagg cggcgcgcgg cgggccgagg atttccgtgc gaggctggaa gcgcgcatcg ggcgtccggt cgacgcggca ttcgccggcg agctgcgcag cggaggcatc gtgacgccca agccgatcgt cggcgaggtc gccggccgtt gcgccgtcat cgtcgacgac ctgatcggta ccgggacgac attggcccgc acggccgaga attgccgcac gctcggcgca caatccgtct acgcggtcgc gacgcatggg atgttctttg atgacgcgcc cgtactgctc ggcggcgatg cgctcgccgg catcgccgtg accgacacgg tgccgccctg gcgcgtgccg gccgggccgc tcaaggacaa gctcgcggtg ctcgacagcg ctccgctgtt tgccgaggcg attgcgcgta tccatcgcgg cggctcgctc accgacctgt tgcggcgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat