SSGCID: Target Page

BumuA.00027.b

Acyl-CoA dehydrogenase domain protein

CENTER ID:	BumuA.00027.b
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00027.b.B1.GE39660	Full length( BumuA.00027.b )		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.1215
RefSeq:	YP_001579233.1
UniProt:	A0A0H3KGQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLDYSPADD AFRADIRAWL EANLPRTLRA KVLDHKRLDR EDYASWHRIL GRRGWSAPAW PAEYGGPGWN ATQRHIWDEE CARIGAPTVL PFGVSMVAPV LMKYGSEAQK RHYLPRILDG TDWWCQGYSE PGSGSDLASL RTRAERRGDH YVVNGQKTWT TLGQHADMMF CLVRTDPSAK KQEGISFLLI DMKTPGITVR PIITLDEDHE VNEVFFEDVK VPVENLVGDE NRGWTYAKYL LGHERTGIAR VGASKRELAF LKRIASNQRK NGKPLLADPV FAAKVAALEV ELMALEVTVL RVVSRETSGK GPGPEASMLK IKGTEVQQAL TELMFEAIGP LAAPFDVPFL EGEREHSIAD DDDAAPLAAY YFNYRKTSIY GGSNEIQKNI IAQMILGL

NT Sequence

ATGGATCTGG ATTATTCCCC CGCCGACGAC GCGTTCCGCG CCGACATCCG CGCGTGGCTC GAGGCGAACC TGCCTCGAAC GCTGCGCGCG AAGGTTCTCG ATCACAAACG ACTCGATCGC GAAGACTACG CGAGCTGGCA CCGCATTCTC GGCCGGCGCG GCTGGTCTGC GCCCGCATGG CCGGCCGAAT ACGGCGGCCC GGGCTGGAAC GCGACGCAGC GACACATCTG GGACGAAGAG TGCGCGCGCA TCGGGGCGCC GACCGTGCTG CCGTTCGGCG TATCGATGGT CGCACCCGTG CTGATGAAAT ACGGCAGCGA GGCACAGAAG CGCCATTACC TGCCGCGCAT CCTCGACGGC ACCGACTGGT GGTGCCAGGG CTACTCGGAG CCGGGTTCCG GGTCGGACCT CGCGTCGCTG CGCACGCGCG CCGAACGTCG CGGCGATCAT TACGTCGTCA ACGGCCAGAA GACGTGGACG ACGCTCGGCC AGCATGCGGA CATGATGTTC TGCCTCGTGC GCACCGATCC GTCCGCGAAA AAGCAGGAAG GCATTTCGTT CCTGCTGATC GACATGAAGA CGCCCGGCAT CACGGTGCGG CCGATCATCA CGCTCGACGA AGATCATGAA GTCAACGAAG TGTTCTTCGA GGACGTGAAG GTGCCGGTCG AGAATCTCGT CGGCGACGAG AACCGCGGCT GGACTTACGC GAAATACCTG CTCGGCCACG AGCGCACGGG GATCGCGCGC GTCGGCGCAT CGAAGCGCGA ACTCGCATTC CTCAAGCGCA TCGCCTCGAA CCAGCGCAAG AACGGCAAGC CGCTGCTCGC CGATCCGGTG TTCGCCGCAA AGGTCGCGGC GCTCGAAGTC GAGCTGATGG CGCTCGAGGT CACGGTGCTG CGCGTCGTGA GCCGCGAGAC GAGCGGCAAG GGGCCGGGGC CGGAAGCGTC GATGCTGAAG ATCAAGGGCA CCGAGGTGCA GCAGGCGCTC ACCGAGCTGA TGTTCGAAGC GATCGGGCCG CTTGCCGCGC CGTTCGACGT GCCGTTTCTC GAAGGCGAGC GCGAGCACAG CATCGCGGAT GACGATGACG CGGCGCCGCT TGCCGCGTAC TACTTCAATT ACCGGAAGAC GTCGATCTAC GGCGGCTCGA ACGAAATTCA GAAAAACATC ATCGCGCAGA TGATTCTCGG CCTC

Details for BumuA.00027.b.B1.GE39660

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/7/2015
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMD LDYSPADDAF RADIRAWLEA NLPRTLRAKV LDHKRLDRED YASWHRILGR RGWSAPAWPA EYGGPGWNAT QRHIWDEECA RIGAPTVLPF GVSMVAPVLM KYGSEAQKRH YLPRILDGTD WWCQGYSEPG SGSDLASLRT RAERRGDHYV VNGQKTWTTL GQHADMMFCL VRTDPSAKKQ EGISFLLIDM KTPGITVRPI ITLDEDHEVN EVFFEDVKVP VENLVGDENR GWTYAKYLLG HERTGIARVG ASKRELAFLK RIASNQRKNG KPLLADPVFA AKVAALEVEL MALEVTVLRV VSRETSGKGP GPEASMLKIK GTEVQQALTE LMFEAIGPLA APFDVPFLEG EREHSIADDD DAAPLAAYYF NYRKTS

Validated NT Sequence

gatcgacgtc ttccggtaat tgaagtagta cgcggcaagc ggcgccgcgt catcgtcatc cgcgatgctg tgctcgcgct cgccttcgag aaacggcacg tcgaacggcg cggcaagcgg cccgatcgct tcgaacatca gctcggtgag cgcctgctgc acctcggtgc ccttgatctt cagcatcgac gcttccggcc ccggcccctt gccgctcgtc tcgcggctca cgacgcgcag caccgtgacc tcgagcgcca tcagctcgac ttcgagcgcc gcgacctttg cggcgaacac cggatcggcg agcagcggct tgccgttctt gcgctggttc gaggcgatgc gcttgaggaa tgcgagttcg cgcttcgatg cgccgacgcg cgcgatcccc gtgcgctcgt ggccgagcag gtatttcgcg taagtccagc cgcggttctc gtcgccgacg agattctcga ccggcacctt cacgtcctcg aagaacactt cgttgacttc atgatcttcg tcgagcgtga tgatcggccg caccgtgatg ccgggcgtct tcatgtcgat cagcaggaac gaaatgcctt cctgcttttt cgcggacgga tcggtgcgca cgaggcagaa catcatgtcc gcatgctggc cgagcgtcgt ccacgtcttc tggccgttga cgacgtaatg atcgccgcga cgttcggcgc gcgtgcgcag cgacgcgagg tccgacccgg aacccggctc cgagtagccc tggcaccacc agtcggtgcc gtcgaggatg cgcggcaggt aatggcgctt ctgtgcctcg ctgccgtatt tcatcagcac gggtgcgacc atcgatacgc cgaacggcag cacggtcggc gccccgatgc gcgcgcactc ttcgtcccag atgtgtcgct gcgtcgcgtt ccagcccggg ccgccgtatt cggccggcca tgcgggcgca gaccagccgc gccggccgag aatgcggtgc cagctcgcgt agtcttcgcg atcgagtcgt ttgtgatcga gaaccttcgc gcgcagcgtt cgaggcaggt tcgcctcgag ccacgcgcgg atgtcggcgc ggaacgcgtc gtcggcgggg gaataatcca gatccatatg gtggtggtgg tggtgagcca tggnnnnntc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tggattattc ccccgccgac gacgcgttcc gcgccgacat ccgcgcgtgg ctcgaggcga acctgcctcg aacgctgcgc gcgaaggttc tcgatcacaa acgactcgat cgcgaagact acgcgagctg gcaccgcatt ctcggccggc gcggctggtc tgcgcccgca tggccggccg aatacggcgg cccgggctgg aacgcgacgc agcgacacat ctgggacgaa gagtgcgcgc gcatcggggc gccgaccgtg ctgccgttcg gcgtatcgat ggtcgcaccc gtgctgatga aatacggcag cgaggcacag aagcgccatt acctgccgcg catcctcgac ggcaccgact ggtggtgcca gggctactcg gagccgggtt ccgggtcgga cctcgcgtcg ctgcgcacgc gcgccgaacg tcgcggcgat cattacgtcg tcaacggcca gaagacgtgg acgacgctcg gccagcatgc ggacatgatg ttctgcctcg tgcgcaccga tccgtccgcg aaaaagcagg aaggcatttc gttcctgctg atcgacatga agacgcccgg catcacggtg cggccgatca tcacgctcga cgaagatcat gaagtcaacg aagtgttctt cgaggacgtg aaggtgccgg tcgagaatct cgtcggcgac gagaaccgcg gctggactta cgcgaaatac ctgctcggcc acgagcgcac ggggatcgcg cgcgtcggcg catcgaagcg cgaactcgca ttcctcaagc gcatcgcctc gaaccagcgc aagaacggca agccgctgct cgccgatccg gtgttcgccg caaaggtcgc ggcgctcgaa gtcgagctga tggcgctcga ggtcacggtg ctgcgcgtcg tgagccgcga gacgagcggc aaggggccgg ggccggaagc gtcgatgctg aagatcaagg gcaccgaggt gcagcaggcg ctcaccgagc tgatgttcga agcgatcggg ccgcttgccg cgccgttcga cgtgccgttt ctcgaaggcg agcgcgagca cagcatcgcg gatgacgatg acgcggcgcc gcttgccgcg tactacttca attaccggaa gacgtcgatc tacggcggct cgaacgaaat tcagaaaaac atcatcgcgc agatgattct cggcctctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at