BumuA.00027.a

Acyl-CoA dehydrogenase domain protein

CENTER ID: BumuA.00027.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00027.a.B1.GE39659 Full length( BumuA.00027.a ) 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.563
RefSeq: YP_001578618.1
UniProt: A0A0H3KMF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFQHTEDRR MLADTLNRFI AEQYPFAVRD RIAQSADGFD RTMWRRFAEL GAVGALFPEA DGGFGGAGFD IAVVFECLGR GLVVEPFLGT LLAGRALSLA GGDAHRAMLA ALIDGSASAA FAHDEPGSHY ELTTVRTRAE RTGDGWVLTG AKGVVDQAAQ ATFFVVSARV AGRDDDAAGI ALFVVPANAA GVSLRDYRKI DGGRAAEVRF ERVALPADAA LGDHDGEQGA ALLERVLGYG LLALSAEALG AMDVAKAHTL DYLRTRKQFG MPIGSFQALQ HRMADLLLEV EQARSAVINA AAQLDAPRDV RERALAAAKY TIGRIGTLVA EESIQLHGGI GMTWELPLSH YAKRLVMIDH QLGDEDHHLA RYIALSKR
NT Sequence
ATGGATTTCC AGCACACAGA AGACCGCCGG ATGCTGGCGG ACACGTTGAA CCGCTTCATC GCCGAGCAGT ATCCGTTCGC GGTGCGCGAC CGCATCGCGC AGTCGGCCGA CGGTTTCGAC CGCACGATGT GGCGGCGCTT TGCCGAACTC GGCGCGGTCG GCGCGCTGTT TCCCGAAGCC GACGGCGGGT TCGGCGGCGC GGGCTTCGAC ATCGCGGTGG TATTCGAATG CCTCGGACGC GGGCTCGTCG TCGAGCCGTT CCTCGGCACG CTGCTCGCGG GGCGCGCACT GTCGCTCGCG GGCGGCGACG CGCATCGCGC GATGCTGGCC GCGCTGATCG ATGGCAGCGC GAGCGCCGCA TTCGCGCACG ACGAGCCGGG CTCGCACTAC GAACTGACGA CGGTGCGCAC GCGTGCCGAG CGCACCGGCG ACGGCTGGGT ATTGACGGGC GCAAAGGGCG TCGTCGACCA GGCCGCGCAG GCGACGTTCT TCGTCGTCAG CGCGCGCGTC GCGGGCCGCG ACGACGACGC GGCCGGCATC GCCCTGTTCG TCGTACCGGC AAACGCGGCG GGCGTGTCGC TGCGCGACTA TCGCAAGATC GACGGCGGCC GCGCGGCCGA GGTGCGCTTC GAGCGCGTCG CGCTGCCGGC CGACGCGGCA CTCGGCGATC ACGACGGCGA GCAGGGCGCG GCGCTGCTCG AGCGCGTGCT CGGGTACGGG CTGCTCGCGC TGTCCGCGGA AGCGCTCGGC GCGATGGACG TCGCGAAGGC GCACACGCTC GACTATCTGC GCACCCGCAA GCAGTTCGGC ATGCCGATCG GCAGCTTTCA GGCGCTGCAG CACCGGATGG CCGACCTGCT GCTCGAAGTC GAGCAGGCGC GCTCGGCCGT GATCAATGCA GCCGCGCAGC TCGACGCGCC GCGCGACGTG CGCGAGCGCG CGCTCGCCGC CGCGAAATAC ACGATCGGCC GGATCGGCAC GCTCGTCGCG GAGGAGAGCA TCCAGCTGCA CGGCGGGATC GGGATGACGT GGGAGCTGCC GCTGTCGCAC TACGCGAAGC GGCTCGTGAT GATCGATCAC CAGCTCGGCG ACGAGGACCA TCATCTCGCG CGCTATATCG CGCTGTCGAA ACGA
Details for BumuA.00027.a.B1.GE39659
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMD FQHTEDRRML ADTLNRFIAE QYPFAVRDRI AQSADGFDRT MWRRFAELGA VGALFPEADG GFGGAGFDIA VVFECLGRGL VVEPFLGTLL AGRALSLAGG DAHRAMLAAL IDGSASAAFA HDEPGSHYEL TTVRTRAERT GDGWVLTGAK GVVDQAAQAT FFVVSARVAG RDDDAAGIAL FVVPANAAGV SLRDYRKIDG GRAAEVRFER VALPADAALG DHDGEQGAAL LERVLGYGLL ALSAEALGAM DVAKAHTLDY LRTRKQFGMP IGSFQALQHR MADLLLEVEQ ARSAVINAAA QLDAPRDVRE RALAAAKYTI GRIGTLVAEE SIQLHGGIGM TWELPLSHYA KRLVMIDHQL GDEDHHLARY IALSKR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tccagcacac agaagaccgc cggatgctgg cggacacgtt gaaccgcttc atcgccgagc agtatccgtt cgcggtgcgc gaccgcatcg cgcagtcggc cgacggtttc gaccgcacga tgtggcggcg ctttgccgaa ctcggcgcgg tcggcgcgct gtttcccgaa gccgacggcg ggttcggcgg cgcgggcttc gacatcgcgg tggtattcga atgcctcgga cgcgggctcg tcgtcgagcc gttcctcggc acgctgctcg cggggcgcgc actgtcgctc gcgggcggcg acgcgcatcg cgcgatgctg gccgcgctga tcgatggcag cgcgagcgcc gcattcgcgc acgacgagcc gggctcgcac tacgaactga cgacggtgcg cacgcgtgcc gagcgcaccg gcgacggctg ggtattgacg ggcgcaaagg gcgtcgtcga ccaggccgcg caggcgacgt tcttcgtcgt cagcgcgcgc gtcgcgggcc gcgacgacga cgcggccggc atcgccctgt tcgtcgtacc ggcaaacgcg gcgggcgtgt cgctgcgcga ctatcgcaag atcgacggcg gccgcgcggc cgaggtgcgc ttcgagcgcg tcgcgctgcc ggccgacgcg gcactcggcg atcacgacgg cgagcagggc gcggcgctgc tcgagcgcgt gctcgggtac gggctgctcg cgctgtccgc ggaagcgctc ggcgcgatgg acgtcgcgaa ggcgcacacg ctcgactatc tgcgcacccg caagcagttc ggcatgccga tcggcagctt tcaggcgctg cagcaccgga tggccgacct gctgctcgaa gtcgagcagg cgcgctcggc cgtgatcaat gcagccgcgc agctcgacgc gccgcgcgac gtgcgcgagc gcgcgctcgc cgccgcgaaa tacacgatcg gccggatcgg cacgctcgtc gcggaggaga gcatccagct gcacggcggg atcgggatga cgtgggagct gccgctgtcg cactacgcga agcggctcgt gatgatcgat caccagctcg gcgacgagga ccatcatctc gcgcgctata tcgcgctgtc gaaacgatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at