SSGCID: Target Page

BumuA.00024.c

Taurine dioxygenase

CENTER ID:	BumuA.00024.c
ORGANISM:	Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00024.c.B1.GE39658	Full length( BumuA.00024.c )		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumuA.00024.c.B1.PS37899	Full length( BumuA.00024.c )		1	291

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|395019.8.peg.6374
RefSeq:	YP_001586000.1
UniProt:	A9AT36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSPASAAPQR FEIVPFDAPL GAEVVGIDLS QPLDADAFAR IRRAHLDHHV LVFRDQRITP DEHVAFSRRF GPLQTHVLHQ FALPGHPEVL IVSNIVENGK PIGLGDAGYF WHSDLSYKRR PSLGSLLHAQ ELPAEGGDTL FANMHLAWDT LPEALRRAVE GRRAEHTYLA RYAELQARSP WRPNLSAEQI AQVEPVVHPV VRTHPETGRR ALFVSEHFTT RIVGLPDDES RALLDELFAH SVRAEHQYRH RWRDHDLVFW DNRSLLHLAA GTPDHLRRKL YRTTIEGDEP F

NT Sequence

GTGTCGCCTG CGTCGGCTGC GCCGCAGCGG TTCGAGATCG TGCCGTTCGA TGCGCCGCTC GGCGCCGAAG TCGTCGGCAT CGACCTGTCG CAGCCGCTCG ACGCCGACGC GTTCGCGCGG ATCCGGCGCG CCCATCTCGA CCATCATGTG CTCGTGTTTC GCGACCAGCG CATCACGCCG GACGAACACG TCGCATTCAG CCGCCGCTTC GGCCCGCTGC AGACCCACGT GCTGCATCAG TTCGCGCTGC CCGGCCATCC GGAAGTGCTG ATCGTGTCGA ACATCGTCGA GAACGGCAAG CCGATCGGCC TTGGCGACGC CGGCTACTTC TGGCATTCCG ACCTGTCGTA CAAGCGCCGC CCGAGCCTCG GCTCGCTGCT GCACGCGCAG GAACTGCCGG CCGAAGGCGG CGATACGTTG TTCGCGAACA TGCACCTCGC GTGGGACACG CTGCCGGAGG CACTGCGGCG CGCCGTCGAA GGGCGCCGCG CGGAGCACAC CTATCTCGCG CGCTATGCGG AGCTGCAGGC GCGCAGCCCG TGGCGGCCGA ACCTGTCCGC CGAGCAGATC GCGCAGGTCG AGCCCGTCGT GCATCCGGTC GTGCGCACGC ACCCGGAGAC GGGCCGCCGC GCGCTGTTCG TCAGCGAGCA TTTCACGACG CGCATCGTCG GGCTGCCCGA CGACGAAAGC CGTGCGCTGC TCGACGAATT GTTCGCGCAC AGCGTGCGCG CCGAGCATCA GTATCGGCAT CGCTGGCGCG ACCACGACCT CGTGTTCTGG GACAACCGGT CGCTGCTGCA TCTGGCCGCC GGCACGCCCG ACCATCTGCG CCGCAAGCTG TACCGCACGA CGATCGAAGG CGACGAACCG TTC

Details for BumuA.00024.c.B1.GE39658

HARVESTED ON:	12/3/2015
SEQUENCED ON:	12/8/2015
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV SPASAAPQRF EIVPFDAPLG AEVVGIDLSQ PLDADAFARI RRAHLDHHVL VFRDQRITPD EHVAFSRRFG PLQTHVLHQF ALPGHPEVLI VSNIVENGKP IGLGDAGYFW HSDLSYKRRP SLGSLLHAQE LPAEGGDTLF ANMHLAWDTL PEALRRAVEG RRAEHTYLAR YAELQARSPW RPNLSAEQIA QVEPVVHPVV RTHPETGRRA LFVSEHFTTR IVGLPDDESR ALLDELFAHS VRAEHQYRHR WRDHDLVFWD NRSLLHLAAG TPDHLRRKLY RXXXEGDEPF

Validated NT Sequence

atggctcacc accaccacca ccatatggtg tcgcctgcgt cggctgcgcc gcagcggttc gagatcgtgc cgttcgatgc gccgctcggc gccgaagtcg tcggcatcga cctgtcgcag ccgctcgacg ccgacgcgtt cgcgcggatc cggcgcgccc atctcgacca tcatgtgctc gtgtttcgcg accagcgcat cacgccggac gaacacgtcg cattcagccg ccgcttcggc ccgctgcaga cccacgtgct gcatcagttc gcgctgcccg gccatccgga agtgctgatc gtgtcgaaca tcgtcgagaa cggcaagccg atcggccttg gcgacgccgg ctacttctgg cattccgacc tgtcgtacaa gcgccgcccg agcctcggct cgctgctgca cgcgcaggaa ctgccggccg aaggcggcga tacgttgttc gcgaacatgc acctcgcgtg ggacacgctg ccggaggcac tgcggcgcgc cgtcgaaggg cgccgcgcgg agcacaccta tctcgcgcgc tatgcggagc tgcaggcgcg cagcccgtgg cggccgaacc tgtccgccga gcagatcgcg caggtcgagc ccgtcgtgca tccggtcgtg cgcacgcacc cggagacggg ccgccgcgcg ctgttcgtca gcgagcattt cacgacgcgc atcgtcgggc tgcccgacga cgaaagccgt gcgctgctcg acgaattgtt cgcgcacagc gtgcgcgccg agcatcagta tcggcatcgc tggcgcgacc acgacctcgt gttctgggac aaccggtcgc tgctgcatct ggccgccggc acgcccgacc atctgcgccg caagctgtac cgcannnnnn ncgaaggcga cgaaccgttc taa

Expected Protein Sequence

MAHHHHHHMS PASAAPQRFE IVPFDAPLGA EVVGIDLSQP LDADAFARIR RAHLDHHVLV FRDQRITPDE HVAFSRRFGP LQTHVLHQFA LPGHPEVLIV SNIVENGKPI GLGDAGYFWH SDLSYKRRPS LGSLLHAQEL PAEGGDTLFA NMHLAWDTLP EALRRAVEGR RAEHTYLARY AELQARSPWR PNLSAEQIAQ VEPVVHPVVR THPETGRRAL FVSEHFTTRI VGLPDDESRA LLDELFAHSV RAEHQYRHRW RDHDLVFWDN RSLLHLAAGT PDHLRRKLYR TTIEGDEPF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcgc ctgcgtcggc tgcgccgcag cggttcgaga tcgtgccgtt cgatgcgccg ctcggcgccg aagtcgtcgg catcgacctg tcgcagccgc tcgacgccga cgcgttcgcg cggatccggc gcgcccatct cgaccatcat gtgctcgtgt ttcgcgacca gcgcatcacg ccggacgaac acgtcgcatt cagccgccgc ttcggcccgc tgcagaccca cgtgctgcat cagttcgcgc tgcccggcca tccggaagtg ctgatcgtgt cgaacatcgt cgagaacggc aagccgatcg gccttggcga cgccggctac ttctggcatt ccgacctgtc gtacaagcgc cgcccgagcc tcggctcgct gctgcacgcg caggaactgc cggccgaagg cggcgatacg ttgttcgcga acatgcacct cgcgtgggac acgctgccgg aggcactgcg gcgcgccgtc gaagggcgcc gcgcggagca cacctatctc gcgcgctatg cggagctgca ggcgcgcagc ccgtggcggc cgaacctgtc cgccgagcag atcgcgcagg tcgagcccgt cgtgcatccg gtcgtgcgca cgcacccgga gacgggccgc cgcgcgctgt tcgtcagcga gcatttcacg acgcgcatcg tcgggctgcc cgacgacgaa agccgtgcgc tgctcgacga attgttcgcg cacagcgtgc gcgccgagca tcagtatcgg catcgctggc gcgaccacga cctcgtgttc tgggacaacc ggtcgctgct gcatctggcc gccggcacgc ccgaccatct gcgccgcaag ctgtaccgca cgacgatcga aggcgacgaa ccgttctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BumuA.00024.c.B1.PS37899

PURIFICATION DATe:	4/6/2016
CONCENTRATION:	32.5mg/ml
OBSERVED MW:	34kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTCGC CTGCGTCGGC TGCGCCGCAG CGGTTCGAGA TCGTGCCGTT CGATGCGCCG CTCGGCGCCG AAGTCGTCGG CATCGACCTG TCGCAGCCGC TCGACGCCGA CGCGTTCGCG CGGATCCGGC GCGCCCATCT CGACCATCAT GTGCTCGTGT TTCGCGACCA GCGCATCACG CCGGACGAAC ACGTCGCATT CAGCCGCCGC TTCGGCCCGC TGCAGACCCA CGTGCTGCAT CAGTTCGCGC TGCCCGGCCA TCCGGAAGTG CTGATCGTGT CGAACATCGT CGAGAACGGC AAGCCGATCG GCCTTGGCGA CGCCGGCTAC TTCTGGCATT CCGACCTGTC GTACAAGCGC CGCCCGAGCC TCGGCTCGCT GCTGCACGCG CAGGAACTGC CGGCCGAAGG CGGCGATACG TTGTTCGCGA ACATGCACCT CGCGTGGGAC ACGCTGCCGG AGGCACTGCG GCGCGCCGTC GAAGGGCGCC GCGCGGAGCA CACCTATCTC GCGCGCTATG CGGAGCTGCA GGCGCGCAGC CCGTGGCGGC CGAACCTGTC CGCCGAGCAG ATCGCGCAGG TCGAGCCCGT CGTGCATCCG GTCGTGCGCA CGCACCCGGA GACGGGCCGC CGCGCGCTGT TCGTCAGCGA GCATTTCACG ACGCGCATCG TCGGGCTGCC CGACGACGAA AGCCGTGCGC TGCTCGACGA ATTGTTCGCG CACAGCGTGC GCGCCGAGCA TCAGTATCGG CATCGCTGGC GCGACCACGA CCTCGTGTTC TGGGACAACC GGTCGCTGCT GCATCTGGCC GCCGGCACGC CCGACCATCT GCGCCGCAAG CTGTACCGCA CGACGATCGA AGGCGACGAA CCGTTCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T