BumuA.00024.b

Taurine dioxygenase

CENTER ID: BumuA.00024.b
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00024.b.B1.GE39657 Full length( BumuA.00024.b ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00024.b.B1.PS37898 Full length( BumuA.00024.b ) 1 315

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4909
RefSeq: YP_001584538.1
UniProt: A0A0H3KL69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSALLEPAGQ PIDVIPLSAH IGAEIRGVDL TQPLTTAQIA AIRHALLKWR VIFFREQFLT HEQHVAFSAQ FGEPTVGHPV FGHVEGHPAV YSIAKHRKAT RFEGEPVRRP WTGWHTDVTA AVNPPWASIL RGVTIPPYGG DTQWTNLVRA YETLSAPLRG FVDGLRGIHR FTPPAGARAT GAFDAAVERR PLVTEHPLVR VHPETGERAL YVSPSFLKSI VGLTPRESQA LLELLWEHVT RPEFTIRFKW EPRSIAFWDN RATAHLAPVD IFDLDFDRQL YRTTLVGDVP VGPDGRPSVA LEGSPVEAAA AVALN
NT Sequence
ATGAGCGCCC TACTCGAACC CGCCGGCCAG CCGATCGACG TGATTCCGCT GTCCGCCCAC ATCGGCGCGG AGATTCGCGG CGTCGACCTG ACCCAGCCGC TGACCACCGC GCAGATCGCC GCGATCCGCC ACGCACTGCT GAAATGGCGC GTGATTTTCT TCCGCGAACA ATTCCTCACG CACGAGCAGC ACGTCGCGTT CTCCGCGCAG TTCGGCGAGC CGACGGTCGG CCATCCGGTG TTCGGTCATG TCGAAGGACA CCCCGCCGTC TACTCGATCG CGAAGCATCG CAAGGCGACG CGCTTCGAAG GCGAACCGGT GCGGCGGCCG TGGACCGGCT GGCATACCGA CGTCACTGCG GCCGTGAATC CGCCGTGGGC GTCGATCCTG CGCGGCGTGA CGATTCCGCC GTACGGCGGC GACACGCAGT GGACCAATCT CGTGCGCGCG TACGAGACGC TGTCCGCGCC GCTGCGCGGC TTCGTCGACG GGCTGCGCGG CATTCACCGC TTCACGCCGC CGGCCGGCGC GCGCGCGACC GGCGCCTTCG ACGCAGCCGT CGAGCGGCGT CCGCTCGTGA CCGAGCACCC GCTCGTGCGC GTCCATCCGG AAACCGGCGA GCGTGCGCTG TACGTGAGTC CGAGTTTCCT GAAATCGATC GTCGGGCTGA CGCCGCGCGA GAGCCAGGCA CTGCTCGAAC TGCTGTGGGA ACACGTGACG CGGCCGGAAT TCACGATCCG CTTCAAGTGG GAACCGCGCA GCATCGCGTT CTGGGACAAC CGCGCAACGG CCCATCTCGC GCCCGTCGAC ATCTTCGATC TCGACTTCGA TCGCCAGCTC TACCGCACCA CGCTCGTCGG CGACGTGCCG GTCGGGCCGG ACGGACGGCC GTCGGTCGCA CTCGAAGGCT CGCCGGTGGA AGCCGCGGCG GCGGTCGCGC TCAAC
Details for BumuA.00024.b.B1.GE39657
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS ALLEPAGQPI DVIPLSAHIG AEIRGVDLTQ PLTTAQIAAI RHALLKWRVI FFREQFLTHE QHVAFSAQFG EPTVGHPVFG HVEGHPAVYS IAKHRKATRF EGEPVRRPWT GWHTDVTAAV NPPWASILRG VTIPPYGGDT QWTNLVRAYE TLSAPLRGFV DGLRGIHRFT PPAGARATGA FDAAVERRPL VTEHPLVRVH PETGERALYV SPSFLKSIVG LTPRESQALL ELLWEHVTRP EFTIRFKWEP RSIAFWDNRA TAHLAPVDIF DLDFDRQLYR TTLVGDVPVG PDGRPSVALE GSPXDAXAAV ALN
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gccctactcg aacccgccgg ccagccgatc gacgtgattc cgctgtccgc ccacatcggc gcggagattc gcggcgtcga cctgacccag ccgctgacca ccgcgcagat cgccgcgatc cgccacgcac tgctgaaatg gcgcgtgatt ttcttccgcg aacaattcct cacgcacgag cagcacgtcg cgttctccgc gcagttcggc gagccgacgg tcggccatcc ggtgttcggt catgtcgaag gacaccccgc cgtctactcg atcgcgaagc atcgcaaggc gacgcgcttc gaaggcgaac cggtgcggcg gccgtggacc ggctggcata ccgacgtcac tgcggccgtg aatccgccgt gggcgtcgat cctgcgcggc gtgacgattc cgccgtacgg cggcgacacg cagtggacca atctcgtgcg cgcgtacgag acgctgtccg cgccgctgcg cggcttcgtc gacgggctgc gcggcattca ccgcttcacg ccgccggccg gcgcgcgcgc gaccggcgcc ttcgacgcag ccgtcgagcg gcgtccgctc gtgaccgagc acccgctcgt gcgcgtccat ccggaaaccg gcgagcgtgc gctgtacgtg agtccgagtt tcctgaaatc gatcgtcggg ctgacgccgc gcgagagcca ggcactgctc gaactgctgt gggaacacgt gacgcggccg gaattcacga tccgcttcaa gtgggaaccg cgcagcatcg cgttctggga caaccgcgca acggcccatc tcgcgcccgt cgacatcttc gatctcgact tcgatcgcca gctctaccgc accacgctcg tcggcgacgt gccggtcggg ccggacggac ggccgtcggt cgcactcgaa ggctcgccgn tngatgccgn ggcggcggtc gcgctcaact aa
Expected Protein Sequence
MAHHHHHHMS ALLEPAGQPI DVIPLSAHIG AEIRGVDLTQ PLTTAQIAAI RHALLKWRVI FFREQFLTHE QHVAFSAQFG EPTVGHPVFG HVEGHPAVYS IAKHRKATRF EGEPVRRPWT GWHTDVTAAV NPPWASILRG VTIPPYGGDT QWTNLVRAYE TLSAPLRGFV DGLRGIHRFT PPAGARATGA FDAAVERRPL VTEHPLVRVH PETGERALYV SPSFLKSIVG LTPRESQALL ELLWEHVTRP EFTIRFKWEP RSIAFWDNRA TAHLAPVDIF DLDFDRQLYR TTLVGDVPVG PDGRPSVALE GSPVEAAAAV ALN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccctactcga acccgccggc cagccgatcg acgtgattcc gctgtccgcc cacatcggcg cggagattcg cggcgtcgac ctgacccagc cgctgaccac cgcgcagatc gccgcgatcc gccacgcact gctgaaatgg cgcgtgattt tcttccgcga acaattcctc acgcacgagc agcacgtcgc gttctccgcg cagttcggcg agccgacggt cggccatccg gtgttcggtc atgtcgaagg acaccccgcc gtctactcga tcgcgaagca tcgcaaggcg acgcgcttcg aaggcgaacc ggtgcggcgg ccgtggaccg gctggcatac cgacgtcact gcggccgtga atccgccgtg ggcgtcgatc ctgcgcggcg tgacgattcc gccgtacggc ggcgacacgc agtggaccaa tctcgtgcgc gcgtacgaga cgctgtccgc gccgctgcgc ggcttcgtcg acgggctgcg cggcattcac cgcttcacgc cgccggccgg cgcgcgcgcg accggcgcct tcgacgcagc cgtcgagcgg cgtccgctcg tgaccgagca cccgctcgtg cgcgtccatc cggaaaccgg cgagcgtgcg ctgtacgtga gtccgagttt cctgaaatcg atcgtcgggc tgacgccgcg cgagagccag gcactgctcg aactgctgtg ggaacacgtg acgcggccgg aattcacgat ccgcttcaag tgggaaccgc gcagcatcgc gttctgggac aaccgcgcaa cggcccatct cgcgcccgtc gacatcttcg atctcgactt cgatcgccag ctctaccgca ccacgctcgt cggcgacgtg ccggtcgggc cggacggacg gccgtcggtc gcactcgaag gctcgccggt ggaagccgcg gcggcggtcg cgctcaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BumuA.00024.b.B1.PS37898
PURIFICATION DATe: 4/5/2016
CONCENTRATION: 33.7mg/ml
OBSERVED MW: 35kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS ALLEPAGQPI DVIPLSAHIG AEIRGVDLTQ PLTTAQIAAI RHALLKWRVI FFREQFLTHE QHVAFSAQFG EPTVGHPVFG HVEGHPAVYS IAKHRKATRF EGEPVRRPWT GWHTDVTAAV NPPWASILRG VTIPPYGGDT QWTNLVRAYE TLSAPLRGFV DGLRGIHRFT PPAGARATGA FDAAVERRPL VTEHPLVRVH PETGERALYV SPSFLKSIVG LTPRESQALL ELLWEHVTRP EFTIRFKWEP RSIAFWDNRA TAHLAPVDIF DLDFDRQLYR TTLVGDVPVG PDGRPSVALE GSPXDAXAAV ALN
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gccctactcg aacccgccgg ccagccgatc gacgtgattc cgctgtccgc ccacatcggc gcggagattc gcggcgtcga cctgacccag ccgctgacca ccgcgcagat cgccgcgatc cgccacgcac tgctgaaatg gcgcgtgatt ttcttccgcg aacaattcct cacgcacgag cagcacgtcg cgttctccgc gcagttcggc gagccgacgg tcggccatcc ggtgttcggt catgtcgaag gacaccccgc cgtctactcg atcgcgaagc atcgcaaggc gacgcgcttc gaaggcgaac cggtgcggcg gccgtggacc ggctggcata ccgacgtcac tgcggccgtg aatccgccgt gggcgtcgat cctgcgcggc gtgacgattc cgccgtacgg cggcgacacg cagtggacca atctcgtgcg cgcgtacgag acgctgtccg cgccgctgcg cggcttcgtc gacgggctgc gcggcattca ccgcttcacg ccgccggccg gcgcgcgcgc gaccggcgcc ttcgacgcag ccgtcgagcg gcgtccgctc gtgaccgagc acccgctcgt gcgcgtccat ccggaaaccg gcgagcgtgc gctgtacgtg agtccgagtt tcctgaaatc gatcgtcggg ctgacgccgc gcgagagcca ggcactgctc gaactgctgt gggaacacgt gacgcggccg gaattcacga tccgcttcaa gtgggaaccg cgcagcatcg cgttctggga caaccgcgca acggcccatc tcgcgcccgt cgacatcttc gatctcgact tcgatcgcca gctctaccgc accacgctcg tcggcgacgt gccggtcggg ccggacggac ggccgtcggt cgcactcgaa ggctcgccgn tngatgccgn ggcggcggtc gcgctcaact aa
Expressed Protein Sequence
MAHHHHHHMS ALLEPAGQPI DVIPLSAHIG AEIRGVDLTQ PLTTAQIAAI RHALLKWRVI FFREQFLTHE QHVAFSAQFG EPTVGHPVFG HVEGHPAVYS IAKHRKATRF EGEPVRRPWT GWHTDVTAAV NPPWASILRG VTIPPYGGDT QWTNLVRAYE TLSAPLRGFV DGLRGIHRFT PPAGARATGA FDAAVERRPL VTEHPLVRVH PETGERALYV SPSFLKSIVG LTPRESQALL ELLWEHVTRP EFTIRFKWEP RSIAFWDNRA TAHLAPVDIF DLDFDRQLYR TTLVGDVPVG PDGRPSVALE GSPVEAAAAV ALN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CCCTACTCGA ACCCGCCGGC CAGCCGATCG ACGTGATTCC GCTGTCCGCC CACATCGGCG CGGAGATTCG CGGCGTCGAC CTGACCCAGC CGCTGACCAC CGCGCAGATC GCCGCGATCC GCCACGCACT GCTGAAATGG CGCGTGATTT TCTTCCGCGA ACAATTCCTC ACGCACGAGC AGCACGTCGC GTTCTCCGCG CAGTTCGGCG AGCCGACGGT CGGCCATCCG GTGTTCGGTC ATGTCGAAGG ACACCCCGCC GTCTACTCGA TCGCGAAGCA TCGCAAGGCG ACGCGCTTCG AAGGCGAACC GGTGCGGCGG CCGTGGACCG GCTGGCATAC CGACGTCACT GCGGCCGTGA ATCCGCCGTG GGCGTCGATC CTGCGCGGCG TGACGATTCC GCCGTACGGC GGCGACACGC AGTGGACCAA TCTCGTGCGC GCGTACGAGA CGCTGTCCGC GCCGCTGCGC GGCTTCGTCG ACGGGCTGCG CGGCATTCAC CGCTTCACGC CGCCGGCCGG CGCGCGCGCG ACCGGCGCCT TCGACGCAGC CGTCGAGCGG CGTCCGCTCG TGACCGAGCA CCCGCTCGTG CGCGTCCATC CGGAAACCGG CGAGCGTGCG CTGTACGTGA GTCCGAGTTT CCTGAAATCG ATCGTCGGGC TGACGCCGCG CGAGAGCCAG GCACTGCTCG AACTGCTGTG GGAACACGTG ACGCGGCCGG AATTCACGAT CCGCTTCAAG TGGGAACCGC GCAGCATCGC GTTCTGGGAC AACCGCGCAA CGGCCCATCT CGCGCCCGTC GACATCTTCG ATCTCGACTT CGATCGCCAG CTCTACCGCA CCACGCTCGT CGGCGACGTG CCGGTCGGGC CGGACGGACG GCCGTCGGTC GCACTCGAAG GCTCGCCGGT GGAAGCCGCG GCGGCGGTCG CGCTCAACTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT