BumuA.00024.a

Taurine dioxygenase

CENTER ID: BumuA.00024.a
ORGANISM: Burkholderia multivorans ATCC 17616
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumuA.00024.a.B1.GE39656 Full length( BumuA.00024.a ) 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|395019.8.peg.4908
RefSeq: YP_001584537.1
UniProt: A0A0H3KU59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEVQHAAHP SSHAAPRTAA APLQLRQVAG RIGAEISGVR LSATLDDATF DAIQAALLRH KVLFFRGQHH LDDAAQEAFA RRFGDTVAHP TVPSVDGSAH LLELDSAHGA RANSWHTDVT FVDAYPKISI LRAVVIPPVG GDTVWANTAA AYAHLPDTLR TLADTLWAVH TNAYDYASTH VHADETQLKR YREVFTSTVY ETEHPVVRVH PETGERTLVL GHFVQRIKGL STQDSAHLLQ VFHEHVTRLE NTVRWSWQEG DVAIWDNRAT QHYAINDYGD ARRVVRRATV HGDVPVGIDG RQSVVLKGPG ATLQ
NT Sequence
ATGTCCGAAG TCCAGCACGC CGCTCACCCG TCATCGCACG CCGCGCCGCG CACGGCCGCT GCCCCGCTCC AGCTTCGTCA GGTCGCCGGC CGGATCGGCG CCGAAATCTC CGGCGTGCGG CTATCGGCCA CGCTCGACGA TGCGACATTC GACGCGATCC AGGCCGCGCT GCTGCGCCAC AAGGTACTGT TCTTCCGCGG CCAGCATCAT CTCGACGATG CCGCGCAGGA AGCGTTCGCG CGCCGTTTCG GCGACACCGT CGCGCATCCG ACCGTGCCGT CCGTCGACGG CAGCGCGCAT CTGCTCGAAC TCGATTCCGC GCACGGCGCG CGCGCCAACT CGTGGCACAC CGACGTGACC TTCGTCGACG CGTATCCGAA GATCTCGATC CTGCGCGCCG TCGTGATCCC GCCCGTCGGC GGCGACACGG TGTGGGCCAA CACGGCCGCC GCGTATGCGC ATCTGCCCGA CACGCTGCGC ACGCTCGCCG ATACGCTGTG GGCCGTCCAT ACGAACGCGT ACGACTACGC GTCGACGCAC GTGCACGCCG ACGAAACGCA GTTGAAGCGC TACCGCGAAG TATTCACCTC GACCGTCTAC GAGACCGAGC ACCCGGTCGT GCGCGTGCAT CCCGAAACGG GCGAGCGCAC GCTCGTGCTC GGCCACTTCG TGCAGCGGAT CAAGGGTCTG TCGACGCAGG ATTCCGCGCA TCTGCTGCAG GTTTTCCACG AACATGTGAC GCGCCTCGAG AACACCGTGC GCTGGAGCTG GCAGGAAGGC GACGTCGCGA TCTGGGACAA CCGCGCGACG CAGCATTACG CGATCAACGA CTACGGCGAT GCGCGCCGCG TCGTGCGGCG TGCGACGGTT CACGGCGACG TGCCGGTCGG CATCGACGGC CGGCAAAGCG TCGTGCTGAA AGGGCCGGGC GCCACGCTGC AG
Details for BumuA.00024.a.B1.GE39656
HARVESTED ON: 12/3/2015
SEQUENCED ON: 12/7/2015
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS XXXHAAHPXS HAAPRTAAAP LQLRQVAGRI GAEISGVRLS ATLDDATFDA IQAALLRHKV LFFRGQHHLD DAAQEAFARR FGDTVAHPTV PSVDGSAHLL ELDSAHGARA NSWHTDVTFV DAYPKISILR AVVIPPVGGD TVWANTAAAY AHLPDTLRTL ADTLWAVHTN AYDYASTHVH ADETQLKRYR EVFTSTVYET EHPVVRVHPE TGERTLVLGH FVQRIKGLST QDSAHLLQVF HEHVTRLENT VRWSWQEGDV AIWDNRATQH YAINDYGDAR RVVRRATVHG DVPVGIDGRQ SVXXXGPG
Validated NT Sequence
ggcgcccggn ccttnnnnnn nnacgctttg ccggccgtcg atgccgaccg gcacgtcgcc gtgaaccgtc gcacgccgca cgacgcggcg cgcatcgccg tagtcgttga tcgcgtaatg ctgcgtcgcg cggttgtccc agatcgcgac gtcgccttcc tgccagctcc agcgcacggt gttctcgagg cgcgtcacat gttcgtggaa aacctgcagc agatgcgcgg aatcctgcgt cgacagaccc ttgatccgct gcacgaagtg gccgagcacg agcgtgcgct cgcccgtttc gggatgcacg cgcacgaccg ggtgctcggt ctcgtagacg gtcgaggtga atacttcgcg gtagcgcttc aactgcgttt cgtcggcgtg cacgtgcgtc gacgcgtagt cgtacgcgtt cgtatggacg gcccacagcg tatcggcgag cgtgcgcagc gtgtcgggca gatgcgcata cgcggcggcc gtgttggccc acaccgtgtc gccgccgacg ggcgggatca cgacggcgcg caggatcgag atcttcggat acgcgtcgac gaaggtcacg tcggtgtgcc acgagttggc gcgcgcgccg tgcgcggaat cgagttcgag cagatgcgcg ctgccgtcga cggacggcac ggtcggatgc gcgacggtgt cgccgaaacg gcgcgcgaac gcttcctgcg cggcatcgtc gagatgatgc tggccgcgga agaacagtac cttgtggcgc agcagcgcgg cctggatcgc gtcgaatgtc gcatcgtcga gcgtggccga tagccgcacg ccggagattt cggcgccgat ccggccggcg acctgacgaa gctggagcgg ggcagcggcc gtgcgcggcg cggcgtgcga tnnngggtga gcggcgtgct nnnnnnngga catatggtgg tggtggtggt gagccatgg
Expected Protein Sequence
MAHHHHHHMS EVQHAAHPSS HAAPRTAAAP LQLRQVAGRI GAEISGVRLS ATLDDATFDA IQAALLRHKV LFFRGQHHLD DAAQEAFARR FGDTVAHPTV PSVDGSAHLL ELDSAHGARA NSWHTDVTFV DAYPKISILR AVVIPPVGGD TVWANTAAAY AHLPDTLRTL ADTLWAVHTN AYDYASTHVH ADETQLKRYR EVFTSTVYET EHPVVRVHPE TGERTLVLGH FVQRIKGLST QDSAHLLQVF HEHVTRLENT VRWSWQEGDV AIWDNRATQH YAINDYGDAR RVVRRATVHG DVPVGIDGRQ SVVLKGPGAT LQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aagtccagca cgccgctcac ccgtcatcgc acgccgcgcc gcgcacggcc gctgccccgc tccagcttcg tcaggtcgcc ggccggatcg gcgccgaaat ctccggcgtg cggctatcgg ccacgctcga cgatgcgaca ttcgacgcga tccaggccgc gctgctgcgc cacaaggtac tgttcttccg cggccagcat catctcgacg atgccgcgca ggaagcgttc gcgcgccgtt tcggcgacac cgtcgcgcat ccgaccgtgc cgtccgtcga cggcagcgcg catctgctcg aactcgattc cgcgcacggc gcgcgcgcca actcgtggca caccgacgtg accttcgtcg acgcgtatcc gaagatctcg atcctgcgcg ccgtcgtgat cccgcccgtc ggcggcgaca cggtgtgggc caacacggcc gccgcgtatg cgcatctgcc cgacacgctg cgcacgctcg ccgatacgct gtgggccgtc catacgaacg cgtacgacta cgcgtcgacg cacgtgcacg ccgacgaaac gcagttgaag cgctaccgcg aagtattcac ctcgaccgtc tacgagaccg agcacccggt cgtgcgcgtg catcccgaaa cgggcgagcg cacgctcgtg ctcggccact tcgtgcagcg gatcaagggt ctgtcgacgc aggattccgc gcatctgctg caggttttcc acgaacatgt gacgcgcctc gagaacaccg tgcgctggag ctggcaggaa ggcgacgtcg cgatctggga caaccgcgcg acgcagcatt acgcgatcaa cgactacggc gatgcgcgcc gcgtcgtgcg gcgtgcgacg gttcacggcg acgtgccggt cggcatcgac ggccggcaaa gcgtcgtgct gaaagggccg ggcgccacgc tgcagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat