BumuA.00020.x
Aldehyde Dehydrogenase
| CENTER ID: | BumuA.00020.x |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00020.x.B1.GE39655 | Full length( BumuA.00020.x ) | 1 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00020.x.B1.PS37849 | Full length( BumuA.00020.x ) | 1 | 477 | ||
| BumuA.00020.x.B1.PS37850 | Full length( BumuA.00020.x ) | 1 | 477 |
Structures
5JRY
DEPOSITED: 5/6/2016
DETERMINATION: XRay
CLONE: BumuA.00020.x.B1.GE39655
PROTEIN: BumuA.00020.x.B1.PS37849
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|395019.8.peg.5269 |
| RefSeq: | YP_001584912.1 |
| UniProt: | A0A0H3KPC8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKETYPYYL ANAAVYANTD LEVTDKYSGK VATRVALADA KAIDAAIGAA VDATKPMREL PAYKRQAVLD HCVARFRERF DELAEALCIE AGKPINDAKG EVTRLIDTFR VASEEAVRID GEVLNLEISA RAQGYTGYTR RVPIGPCSFI SPFNFPLNLA AHKVAPALAA GCPFVLKPAS RTPVGALIIG EVLAETDLPK GAFSVLPAHR DGADLFTTDD RFRLLSFTGS PAVGWALKEK AGKKKVVLEL GGNAAAIVDA DQLDRLDYVV DRLAFGAFYQ SGQSCIGVQR ILAHADIYDA LRDKLIAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI VAGGKVDGAM FEATLLENVG RDQDLYRKEA FGPIAILEKF DRFDDALARV NDSDFGLQAG VFTDSLAHTQ QAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPR LLVVRRR
NT Sequence
ATGCTGAAGG AAACCTATCC GTACTACCTG GCCAACGCGG CCGTCTACGC GAACACCGAC CTCGAGGTCA CGGACAAGTA CAGCGGCAAG GTCGCGACGC GCGTCGCGCT CGCCGATGCG AAAGCGATCG ACGCGGCGAT CGGCGCGGCC GTCGACGCAA CCAAGCCGAT GCGCGAACTG CCCGCGTACA AGCGGCAGGC CGTGCTCGAC CATTGCGTCG CGCGCTTTCG CGAGCGCTTC GACGAACTGG CGGAAGCGCT CTGCATCGAG GCCGGCAAGC CGATCAACGA CGCGAAGGGC GAGGTCACGC GACTGATCGA CACGTTCCGC GTCGCATCCG AGGAAGCGGT GCGTATCGAC GGCGAAGTGC TGAACCTCGA GATCTCGGCG CGTGCGCAGG GCTATACGGG CTATACGCGG CGCGTGCCGA TCGGCCCGTG CTCGTTCATC TCGCCGTTCA ACTTTCCGTT GAACCTCGCC GCACACAAGG TCGCGCCCGC ACTCGCGGCC GGCTGCCCGT TCGTGCTGAA GCCCGCGAGC CGCACGCCGG TCGGCGCGCT GATCATCGGC GAAGTGCTCG CGGAAACCGA TCTGCCGAAG GGCGCGTTCT CGGTGCTGCC CGCGCACCGC GACGGCGCGG ATCTGTTCAC GACCGACGAC CGTTTCCGGC TGTTGTCGTT TACGGGTTCG CCGGCCGTCG GCTGGGCGCT GAAGGAGAAG GCCGGCAAGA AGAAAGTCGT GCTCGAGCTC GGCGGCAACG CGGCCGCGAT CGTCGACGCG GACCAGCTCG ACCGGCTCGA CTATGTGGTC GATCGCCTCG CGTTCGGCGC GTTCTATCAG TCGGGCCAGA GCTGCATCGG CGTGCAGCGC ATCCTCGCGC ATGCGGACAT CTACGACGCG CTGCGCGACA AGCTGATCGC GAAGACGCGC TCGCTGAAGA TGGGCGATCC GAAGGATCCG TCGACGTTCG TCGGCCCGAT GATTTCCGAG TCCGAATCGC GGCGGCTGTC CGGCTGGATG GACGCGGCCG TGGCGGCCGG CGCGAAGATC GTCGCGGGCG GCAAGGTCGA CGGCGCGATG TTCGAGGCGA CGCTGCTCGA AAACGTCGGC CGCGACCAGG ACCTGTACCG CAAGGAGGCG TTCGGCCCGA TCGCGATCCT CGAGAAGTTC GACCGCTTCG ACGACGCGCT CGCGCGCGTG AACGACAGCG ATTTCGGGCT GCAGGCCGGC GTGTTCACCG ATTCGCTCGC GCATACGCAG CAGGCGTGGG ACGAACTCGA AGTCGGCGGC GTCGTGATCA ACGACGTGCC GTCGTTCCGC GTCGACAACA TGCCGTACGG CGGCGTGAAG GATTCGGGGC TCGGCCGCGA AGGGATTCGC TACGCGATCG AGGACATGAC CGAGCCGCGC CTGCTTGTCG TACGGCGTCG C
Details for BumuA.00020.x.B1.GE39655
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH XGRXPHSRPA ARSC*SPRAR TPVGALIIGE VLAETDLPKG AFSGAARAPR RRGSVHDRRP XSGCCRLRVR RPSAGRLKEK AGKKKVVLEL GGNAAAIVDA DQLDRLDYVV DRLAFGAFYQ SGQSCIGVQR ILAHADIYDA LRDKLIAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI VAGGKVDGAM FEATLLENVG RDQDLYRKEA FGPIAILEKF DRFDDALARV NDSDFGLQAG VFTDSLAHTQ QAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPX LLVVRRR
Validated NT Sequence
atggctcacc accaccacca ccatatgctg aaggaaacct atccgtacta cctggccaac gcggccgtct acgcgaacac cgacctcgag gtcacggaca agtacagcgg caaggtcgcg acgcgcgtcg cgctcgccga tgcgaaagcg atcgacgcgg cgatcggcgc ggccgtcgac gcaaccaagc cgatgcgcga actgcccgcg tacaagcggc aggccgtgct cgaccattgc gtcgcgcgct ttcgcgagcg cttcgacgaa ctggcggaag cgctctgcat cgaggccggc aagccgatca acgacgcgaa gggcgaggtc acgcgactga tcgacacgtt ccgcgtcgca tccgaggaag cggtgcgtat cgacggcgaa gtgctgaacc tcgagatctc ggcgcgtgcg cagggctata cgggctatac gcggcgcgtg ccgatcggcc cgtgctcgtt catctcgccg ttcaactttc cgttgaacct cgccgcacac naaggtcgcg ncccgcactc gcggccggct gcccgttcgt gctgaagccc gcgagcccgc acgccggtcg gcgcgctgat catcggcgaa gtgctcgcgg aaaccgatct gccgaagggc gcgttctcgg gtgctgcccg cgcaccgcga cggcgcggat ctgttcacga ccga-cgacc gntttccggc tgttgtcgtt tacgggttcg ccggccgtcg gctgggcgct tgaaggagaa ggccggcaag aagaaagtcg tgctcgagct cggcggcaac gcggccgcga tcgtcgacgc ggaccagctc gaccggctcg actatgtggt cgatcgcctc gcgttcggcg cgttctatca gtcgggccag agctgcatcg gcgtgcagcg catcctcgcg catgcggaca tctacgacgc gctgcgcgac aagctgatcg cgaagacgcg ctcgctgaag atgggcgatc cgaaggatcc gtcgacgttc gtcggcccga tgatttccga gtccgaatcg cggcggctgt ccggctggat ggacgcggcc gtggcggccg gcgcgaagat cgtcgcgggc ggcaaggtcg acggcgcgat gttcgaggcg acgctgctcg aaaacgtcgg ccgcgaccag gacctgtacc gcaaggaggc gttcggcccg atcgcgatcc tcgagaagtt cgaccgcttc gacgacgcgc tcgcgcgcgt gaacgacagc gatttcgggc tgcaggccgg cgtgttcacc gattcgctcg cgcatacgca gcaggcgtgg gacgaactcg aagtcggcgg cgtcgtgatc aacgacgtgc cgtcgttccg cgtcgacaac atgccgtacg gcggcgtgaa ggattcgggg ctcggccgcg aagggattcg ctacgcgatc gaggacatga ccgagccgnn nctgcttgtc gtacggcgtc gctaa
Expected Protein Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH KVAPALAAGC PFVLKPASRT PVGALIIGEV LAETDLPKGA FSVLPAHRDG ADLFTTDDRF RLLSFTGSPA VGWALKEKAG KKKVVLELGG NAAAIVDADQ LDRLDYVVDR LAFGAFYQSG QSCIGVQRIL AHADIYDALR DKLIAKTRSL KMGDPKDPST FVGPMISESE SRRLSGWMDA AVAAGAKIVA GGKVDGAMFE ATLLENVGRD QDLYRKEAFG PIAILEKFDR FDDALARVND SDFGLQAGVF TDSLAHTQQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLL VVRRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga aggaaaccta tccgtactac ctggccaacg cggccgtcta cgcgaacacc gacctcgagg tcacggacaa gtacagcggc aaggtcgcga cgcgcgtcgc gctcgccgat gcgaaagcga tcgacgcggc gatcggcgcg gccgtcgacg caaccaagcc gatgcgcgaa ctgcccgcgt acaagcggca ggccgtgctc gaccattgcg tcgcgcgctt tcgcgagcgc ttcgacgaac tggcggaagc gctctgcatc gaggccggca agccgatcaa cgacgcgaag ggcgaggtca cgcgactgat cgacacgttc cgcgtcgcat ccgaggaagc ggtgcgtatc gacggcgaag tgctgaacct cgagatctcg gcgcgtgcgc agggctatac gggctatacg cggcgcgtgc cgatcggccc gtgctcgttc atctcgccgt tcaactttcc gttgaacctc gccgcacaca aggtcgcgcc cgcactcgcg gccggctgcc cgttcgtgct gaagcccgcg agccgcacgc cggtcggcgc gctgatcatc ggcgaagtgc tcgcggaaac cgatctgccg aagggcgcgt tctcggtgct gcccgcgcac cgcgacggcg cggatctgtt cacgaccgac gaccgtttcc ggctgttgtc gtttacgggt tcgccggccg tcggctgggc gctgaaggag aaggccggca agaagaaagt cgtgctcgag ctcggcggca acgcggccgc gatcgtcgac gcggaccagc tcgaccggct cgactatgtg gtcgatcgcc tcgcgttcgg cgcgttctat cagtcgggcc agagctgcat cggcgtgcag cgcatcctcg cgcatgcgga catctacgac gcgctgcgcg acaagctgat cgcgaagacg cgctcgctga agatgggcga tccgaaggat ccgtcgacgt tcgtcggccc gatgatttcc gagtccgaat cgcggcggct gtccggctgg atggacgcgg ccgtggcggc cggcgcgaag atcgtcgcgg gcggcaaggt cgacggcgcg atgttcgagg cgacgctgct cgaaaacgtc ggccgcgacc aggacctgta ccgcaaggag gcgttcggcc cgatcgcgat cctcgagaag ttcgaccgct tcgacgacgc gctcgcgcgc gtgaacgaca gcgatttcgg gctgcaggcc ggcgtgttca ccgattcgct cgcgcatacg cagcaggcgt gggacgaact cgaagtcggc ggcgtcgtga tcaacgacgt gccgtcgttc cgcgtcgaca acatgccgta cggcggcgtg aaggattcgg ggctcggccg cgaagggatt cgctacgcga tcgaggacat gaccgagccg cgcctgcttg tcgtacggcg tcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BumuA.00020.x.B1.PS37849
| PURIFICATION DATe: | 2/24/2016 |
| CONCENTRATION: | 60mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH XGRXPHSRPA ARSC*SPRAR TPVGALIIGE VLAETDLPKG AFSGAARAPR RRGSVHDRRP XSGCCRLRVR RPSAGRLKEK AGKKKVVLEL GGNAAAIVDA DQLDRLDYVV DRLAFGAFYQ SGQSCIGVQR ILAHADIYDA LRDKLIAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI VAGGKVDGAM FEATLLENVG RDQDLYRKEA FGPIAILEKF DRFDDALARV NDSDFGLQAG VFTDSLAHTQ QAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPX LLVVRRR
Validated NT Sequence
atggctcacc accaccacca ccatatgctg aaggaaacct atccgtacta cctggccaac gcggccgtct acgcgaacac cgacctcgag gtcacggaca agtacagcgg caaggtcgcg acgcgcgtcg cgctcgccga tgcgaaagcg atcgacgcgg cgatcggcgc ggccgtcgac gcaaccaagc cgatgcgcga actgcccgcg tacaagcggc aggccgtgct cgaccattgc gtcgcgcgct ttcgcgagcg cttcgacgaa ctggcggaag cgctctgcat cgaggccggc aagccgatca acgacgcgaa gggcgaggtc acgcgactga tcgacacgtt ccgcgtcgca tccgaggaag cggtgcgtat cgacggcgaa gtgctgaacc tcgagatctc ggcgcgtgcg cagggctata cgggctatac gcggcgcgtg ccgatcggcc cgtgctcgtt catctcgccg ttcaactttc cgttgaacct cgccgcacac naaggtcgcg ncccgcactc gcggccggct gcccgttcgt gctgaagccc gcgagcccgc acgccggtcg gcgcgctgat catcggcgaa gtgctcgcgg aaaccgatct gccgaagggc gcgttctcgg gtgctgcccg cgcaccgcga cggcgcggat ctgttcacga ccga-cgacc gntttccggc tgttgtcgtt tacgggttcg ccggccgtcg gctgggcgct tgaaggagaa ggccggcaag aagaaagtcg tgctcgagct cggcggcaac gcggccgcga tcgtcgacgc ggaccagctc gaccggctcg actatgtggt cgatcgcctc gcgttcggcg cgttctatca gtcgggccag agctgcatcg gcgtgcagcg catcctcgcg catgcggaca tctacgacgc gctgcgcgac aagctgatcg cgaagacgcg ctcgctgaag atgggcgatc cgaaggatcc gtcgacgttc gtcggcccga tgatttccga gtccgaatcg cggcggctgt ccggctggat ggacgcggcc gtggcggccg gcgcgaagat cgtcgcgggc ggcaaggtcg acggcgcgat gttcgaggcg acgctgctcg aaaacgtcgg ccgcgaccag gacctgtacc gcaaggaggc gttcggcccg atcgcgatcc tcgagaagtt cgaccgcttc gacgacgcgc tcgcgcgcgt gaacgacagc gatttcgggc tgcaggccgg cgtgttcacc gattcgctcg cgcatacgca gcaggcgtgg gacgaactcg aagtcggcgg cgtcgtgatc aacgacgtgc cgtcgttccg cgtcgacaac atgccgtacg gcggcgtgaa ggattcgggg ctcggccgcg aagggattcg ctacgcgatc gaggacatga ccgagccgnn nctgcttgtc gtacggcgtc gctaa
Expressed Protein Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH KVAPALAAGC PFVLKPASRT PVGALIIGEV LAETDLPKGA FSVLPAHRDG ADLFTTDDRF RLLSFTGSPA VGWALKEKAG KKKVVLELGG NAAAIVDADQ LDRLDYVVDR LAFGAFYQSG QSCIGVQRIL AHADIYDALR DKLIAKTRSL KMGDPKDPST FVGPMISESE SRRLSGWMDA AVAAGAKIVA GGKVDGAMFE ATLLENVGRD QDLYRKEAFG PIAILEKFDR FDDALARVND SDFGLQAGVF TDSLAHTQQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLL VVRRR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTGA AGGAAACCTA TCCGTACTAC CTGGCCAACG CGGCCGTCTA CGCGAACACC GACCTCGAGG TCACGGACAA GTACAGCGGC AAGGTCGCGA CGCGCGTCGC GCTCGCCGAT GCGAAAGCGA TCGACGCGGC GATCGGCGCG GCCGTCGACG CAACCAAGCC GATGCGCGAA CTGCCCGCGT ACAAGCGGCA GGCCGTGCTC GACCATTGCG TCGCGCGCTT TCGCGAGCGC TTCGACGAAC TGGCGGAAGC GCTCTGCATC GAGGCCGGCA AGCCGATCAA CGACGCGAAG GGCGAGGTCA CGCGACTGAT CGACACGTTC CGCGTCGCAT CCGAGGAAGC GGTGCGTATC GACGGCGAAG TGCTGAACCT CGAGATCTCG GCGCGTGCGC AGGGCTATAC GGGCTATACG CGGCGCGTGC CGATCGGCCC GTGCTCGTTC ATCTCGCCGT TCAACTTTCC GTTGAACCTC GCCGCACACA AGGTCGCGCC CGCACTCGCG GCCGGCTGCC CGTTCGTGCT GAAGCCCGCG AGCCGCACGC CGGTCGGCGC GCTGATCATC GGCGAAGTGC TCGCGGAAAC CGATCTGCCG AAGGGCGCGT TCTCGGTGCT GCCCGCGCAC CGCGACGGCG CGGATCTGTT CACGACCGAC GACCGTTTCC GGCTGTTGTC GTTTACGGGT TCGCCGGCCG TCGGCTGGGC GCTGAAGGAG AAGGCCGGCA AGAAGAAAGT CGTGCTCGAG CTCGGCGGCA ACGCGGCCGC GATCGTCGAC GCGGACCAGC TCGACCGGCT CGACTATGTG GTCGATCGCC TCGCGTTCGG CGCGTTCTAT CAGTCGGGCC AGAGCTGCAT CGGCGTGCAG CGCATCCTCG CGCATGCGGA CATCTACGAC GCGCTGCGCG ACAAGCTGAT CGCGAAGACG CGCTCGCTGA AGATGGGCGA TCCGAAGGAT CCGTCGACGT TCGTCGGCCC GATGATTTCC GAGTCCGAAT CGCGGCGGCT GTCCGGCTGG ATGGACGCGG CCGTGGCGGC CGGCGCGAAG ATCGTCGCGG GCGGCAAGGT CGACGGCGCG ATGTTCGAGG CGACGCTGCT CGAAAACGTC GGCCGCGACC AGGACCTGTA CCGCAAGGAG GCGTTCGGCC CGATCGCGAT CCTCGAGAAG TTCGACCGCT TCGACGACGC GCTCGCGCGC GTGAACGACA GCGATTTCGG GCTGCAGGCC GGCGTGTTCA CCGATTCGCT CGCGCATACG CAGCAGGCGT GGGACGAACT CGAAGTCGGC GGCGTCGTGA TCAACGACGT GCCGTCGTTC CGCGTCGACA ACATGCCGTA CGGCGGCGTG AAGGATTCGG GGCTCGGCCG CGAAGGGATT CGCTACGCGA TCGAGGACAT GACCGAGCCG CGCCTGCTTG TCGTACGGCG TCGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT
Details for BumuA.00020.x.B1.PS37850
| PURIFICATION DATe: | 2/24/2016 |
| CONCENTRATION: | 35.1mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH XGRXPHSRPA ARSC*SPRAR TPVGALIIGE VLAETDLPKG AFSGAARAPR RRGSVHDRRP XSGCCRLRVR RPSAGRLKEK AGKKKVVLEL GGNAAAIVDA DQLDRLDYVV DRLAFGAFYQ SGQSCIGVQR ILAHADIYDA LRDKLIAKTR SLKMGDPKDP STFVGPMISE SESRRLSGWM DAAVAAGAKI VAGGKVDGAM FEATLLENVG RDQDLYRKEA FGPIAILEKF DRFDDALARV NDSDFGLQAG VFTDSLAHTQ QAWDELEVGG VVINDVPSFR VDNMPYGGVK DSGLGREGIR YAIEDMTEPX LLVVRRR
Validated NT Sequence
atggctcacc accaccacca ccatatgctg aaggaaacct atccgtacta cctggccaac gcggccgtct acgcgaacac cgacctcgag gtcacggaca agtacagcgg caaggtcgcg acgcgcgtcg cgctcgccga tgcgaaagcg atcgacgcgg cgatcggcgc ggccgtcgac gcaaccaagc cgatgcgcga actgcccgcg tacaagcggc aggccgtgct cgaccattgc gtcgcgcgct ttcgcgagcg cttcgacgaa ctggcggaag cgctctgcat cgaggccggc aagccgatca acgacgcgaa gggcgaggtc acgcgactga tcgacacgtt ccgcgtcgca tccgaggaag cggtgcgtat cgacggcgaa gtgctgaacc tcgagatctc ggcgcgtgcg cagggctata cgggctatac gcggcgcgtg ccgatcggcc cgtgctcgtt catctcgccg ttcaactttc cgttgaacct cgccgcacac naaggtcgcg ncccgcactc gcggccggct gcccgttcgt gctgaagccc gcgagcccgc acgccggtcg gcgcgctgat catcggcgaa gtgctcgcgg aaaccgatct gccgaagggc gcgttctcgg gtgctgcccg cgcaccgcga cggcgcggat ctgttcacga ccga-cgacc gntttccggc tgttgtcgtt tacgggttcg ccggccgtcg gctgggcgct tgaaggagaa ggccggcaag aagaaagtcg tgctcgagct cggcggcaac gcggccgcga tcgtcgacgc ggaccagctc gaccggctcg actatgtggt cgatcgcctc gcgttcggcg cgttctatca gtcgggccag agctgcatcg gcgtgcagcg catcctcgcg catgcggaca tctacgacgc gctgcgcgac aagctgatcg cgaagacgcg ctcgctgaag atgggcgatc cgaaggatcc gtcgacgttc gtcggcccga tgatttccga gtccgaatcg cggcggctgt ccggctggat ggacgcggcc gtggcggccg gcgcgaagat cgtcgcgggc ggcaaggtcg acggcgcgat gttcgaggcg acgctgctcg aaaacgtcgg ccgcgaccag gacctgtacc gcaaggaggc gttcggcccg atcgcgatcc tcgagaagtt cgaccgcttc gacgacgcgc tcgcgcgcgt gaacgacagc gatttcgggc tgcaggccgg cgtgttcacc gattcgctcg cgcatacgca gcaggcgtgg gacgaactcg aagtcggcgg cgtcgtgatc aacgacgtgc cgtcgttccg cgtcgacaac atgccgtacg gcggcgtgaa ggattcgggg ctcggccgcg aagggattcg ctacgcgatc gaggacatga ccgagccgnn nctgcttgtc gtacggcgtc gctaa
Expressed Protein Sequence
MAHHHHHHML KETYPYYLAN AAVYANTDLE VTDKYSGKVA TRVALADAKA IDAAIGAAVD ATKPMRELPA YKRQAVLDHC VARFRERFDE LAEALCIEAG KPINDAKGEV TRLIDTFRVA SEEAVRIDGE VLNLEISARA QGYTGYTRRV PIGPCSFISP FNFPLNLAAH KVAPALAAGC PFVLKPASRT PVGALIIGEV LAETDLPKGA FSVLPAHRDG ADLFTTDDRF RLLSFTGSPA VGWALKEKAG KKKVVLELGG NAAAIVDADQ LDRLDYVVDR LAFGAFYQSG QSCIGVQRIL AHADIYDALR DKLIAKTRSL KMGDPKDPST FVGPMISESE SRRLSGWMDA AVAAGAKIVA GGKVDGAMFE ATLLENVGRD QDLYRKEAFG PIAILEKFDR FDDALARVND SDFGLQAGVF TDSLAHTQQA WDELEVGGVV INDVPSFRVD NMPYGGVKDS GLGREGIRYA IEDMTEPRLL VVRRR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTGA AGGAAACCTA TCCGTACTAC CTGGCCAACG CGGCCGTCTA CGCGAACACC GACCTCGAGG TCACGGACAA GTACAGCGGC AAGGTCGCGA CGCGCGTCGC GCTCGCCGAT GCGAAAGCGA TCGACGCGGC GATCGGCGCG GCCGTCGACG CAACCAAGCC GATGCGCGAA CTGCCCGCGT ACAAGCGGCA GGCCGTGCTC GACCATTGCG TCGCGCGCTT TCGCGAGCGC TTCGACGAAC TGGCGGAAGC GCTCTGCATC GAGGCCGGCA AGCCGATCAA CGACGCGAAG GGCGAGGTCA CGCGACTGAT CGACACGTTC CGCGTCGCAT CCGAGGAAGC GGTGCGTATC GACGGCGAAG TGCTGAACCT CGAGATCTCG GCGCGTGCGC AGGGCTATAC GGGCTATACG CGGCGCGTGC CGATCGGCCC GTGCTCGTTC ATCTCGCCGT TCAACTTTCC GTTGAACCTC GCCGCACACA AGGTCGCGCC CGCACTCGCG GCCGGCTGCC CGTTCGTGCT GAAGCCCGCG AGCCGCACGC CGGTCGGCGC GCTGATCATC GGCGAAGTGC TCGCGGAAAC CGATCTGCCG AAGGGCGCGT TCTCGGTGCT GCCCGCGCAC CGCGACGGCG CGGATCTGTT CACGACCGAC GACCGTTTCC GGCTGTTGTC GTTTACGGGT TCGCCGGCCG TCGGCTGGGC GCTGAAGGAG AAGGCCGGCA AGAAGAAAGT CGTGCTCGAG CTCGGCGGCA ACGCGGCCGC GATCGTCGAC GCGGACCAGC TCGACCGGCT CGACTATGTG GTCGATCGCC TCGCGTTCGG CGCGTTCTAT CAGTCGGGCC AGAGCTGCAT CGGCGTGCAG CGCATCCTCG CGCATGCGGA CATCTACGAC GCGCTGCGCG ACAAGCTGAT CGCGAAGACG CGCTCGCTGA AGATGGGCGA TCCGAAGGAT CCGTCGACGT TCGTCGGCCC GATGATTTCC GAGTCCGAAT CGCGGCGGCT GTCCGGCTGG ATGGACGCGG CCGTGGCGGC CGGCGCGAAG ATCGTCGCGG GCGGCAAGGT CGACGGCGCG ATGTTCGAGG CGACGCTGCT CGAAAACGTC GGCCGCGACC AGGACCTGTA CCGCAAGGAG GCGTTCGGCC CGATCGCGAT CCTCGAGAAG TTCGACCGCT TCGACGACGC GCTCGCGCGC GTGAACGACA GCGATTTCGG GCTGCAGGCC GGCGTGTTCA CCGATTCGCT CGCGCATACG CAGCAGGCGT GGGACGAACT CGAAGTCGGC GGCGTCGTGA TCAACGACGT GCCGTCGTTC CGCGTCGACA ACATGCCGTA CGGCGGCGTG AAGGATTCGG GGCTCGGCCG CGAAGGGATT CGCTACGCGA TCGAGGACAT GACCGAGCCG CGCCTGCTTG TCGTACGGCG TCGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT