BumuA.00020.h
NAD-dependent aldehyde dehydrogenase
| CENTER ID: | BumuA.00020.h |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00020.h.B1.GE39653 | Full length( BumuA.00020.h ) | 1 | 568 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00020.h.B1.PS37903 | Full length( BumuA.00020.h ) | 1 | 568 |
Structures
5UJU
DEPOSITED: 1/19/2017
DETERMINATION: XRay
CLONE: BumuA.00020.h.B1.GE39653
PROTEIN: BumuA.00020.h.B1.PS37903
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|395019.8.peg.3082 |
| RefSeq: | YP_001581032.1 |
| UniProt: | A0A0H3KBY8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHALFTKHE DTLKRALAAI ESRGYWSPFA EMPSPKVYGE SGNADGEAAF KSHLGKTFEL DQPASGETVG AERSPYGVAL DIRYPKSTPD ALIAAAAAAQ RTWREAGPSA WIGVSLEILA RLNRASFEIA YSVMHTTGQA FMMAFQAGGP HAQDRALEAV AYAWDQLRRI PADAHWEKPQ GKNPPLAMQK RYTIVPRGTG LVLGCCTFPT WNGYPGLFAD LATGNTVIVK PHPGAILPLA ITVRIARDVL REAGFDPNVV TLLATEPNDG ALVQDLALRP EIKLIDFTGS TQNGTWLERH AHQAQVYTEK AGVNQIVIDS TDDLKAAAKN IAFSLALYSG QMCTAPQNIY VPRDGIRTAD GHASFDEVAQ AIAGAVQKLT GDPARSVELI GAIQNDGVTA RIDAARAVGR VLLDSQTLQH PAFPDARVRT PLVLQLDVAD REKFTQEWFG PISFVIATDS TAQSLDLAGE IAAEHGALTL SVYSTADDVI DAAHEAAVRG GVALSINLTG GVFVNQSAAF SDFHGTGANP AANAALADAA FVANRFRVVQ SRVHVAPKAA PAEVGQPA
NT Sequence
ATGACCCATG CACTGTTCAC GAAGCACGAA GACACGCTGA AACGCGCACT CGCCGCCATC GAGAGCCGCG GATACTGGAG CCCGTTCGCC GAAATGCCGA GCCCCAAAGT GTACGGGGAA AGCGGCAATG CAGACGGCGA AGCCGCGTTC AAATCGCACC TCGGCAAGAC CTTCGAGCTC GACCAGCCGG CCAGCGGCGA GACGGTCGGC GCGGAACGTT CTCCGTATGG CGTCGCGCTC GACATCCGGT ATCCGAAGTC GACGCCCGAT GCGCTGATCG CAGCCGCCGC CGCTGCCCAA CGCACGTGGC GCGAAGCCGG CCCGAGCGCG TGGATCGGTG TCAGCCTCGA GATCCTCGCG CGCCTGAACC GCGCCAGTTT CGAAATCGCG TACAGCGTGA TGCATACGAC GGGCCAGGCA TTCATGATGG CGTTCCAGGC CGGCGGCCCG CATGCGCAGG ACCGCGCGCT CGAGGCCGTC GCCTATGCGT GGGACCAGCT GCGCCGCATC CCGGCCGACG CGCATTGGGA AAAGCCGCAA GGCAAGAACC CGCCGCTCGC GATGCAGAAG CGTTACACGA TCGTCCCGCG CGGCACCGGG CTCGTGCTCG GCTGCTGCAC GTTCCCGACC TGGAACGGTT ATCCCGGCCT GTTCGCCGAT CTCGCGACCG GCAACACGGT CATCGTCAAG CCTCATCCGG GCGCGATCCT GCCGCTCGCG ATCACCGTGC GGATCGCGCG CGACGTGCTG CGCGAGGCCG GCTTCGATCC TAACGTCGTG ACGCTGCTCG CCACGGAGCC GAACGACGGC GCACTCGTGC AGGACCTCGC GCTGCGCCCC GAGATCAAGC TGATCGACTT CACCGGCAGC ACGCAGAACG GCACGTGGCT CGAACGTCAC GCCCATCAGG CGCAGGTCTA CACCGAGAAG GCAGGCGTCA ATCAGATCGT GATCGACTCG ACCGACGATC TGAAAGCCGC CGCCAAGAAC ATCGCGTTCT CGCTCGCGCT GTACTCGGGG CAGATGTGCA CCGCCCCGCA GAACATCTAT GTGCCGCGCG ACGGCATCCG CACCGCCGAC GGCCATGCGA GCTTCGACGA AGTCGCGCAG GCGATCGCCG GCGCAGTGCA GAAACTCACC GGCGACCCGG CACGGTCGGT CGAGCTGATC GGCGCCATCC AGAACGACGG CGTGACGGCA CGCATCGACG CCGCGCGCGC AGTCGGCCGC GTGCTCCTCG ATAGTCAGAC GCTTCAGCAC CCCGCCTTCC CCGACGCTCG CGTGCGCACG CCGCTCGTGC TGCAGCTCGA CGTCGCCGAC CGCGAAAAAT TCACGCAGGA ATGGTTCGGG CCGATCTCGT TCGTGATCGC GACCGACTCG ACCGCGCAGT CGCTCGATCT CGCCGGCGAG ATCGCCGCCG AGCACGGCGC GCTGACGCTG TCCGTCTACA GCACGGCCGA CGACGTGATC GACGCCGCAC ACGAAGCCGC GGTGCGCGGC GGCGTCGCAC TGTCGATCAA CCTGACGGGC GGCGTGTTCG TCAATCAGTC GGCGGCGTTC TCTGACTTCC ACGGTACGGG CGCCAATCCG GCCGCGAACG CTGCGCTGGC CGATGCCGCA TTCGTCGCCA ACCGCTTCCG CGTGGTTCAG AGCCGCGTCC ATGTTGCGCC GAAGGCGGCG CCCGCGGAAG TCGGCCAACC GGCA
Details for BumuA.00020.h.B1.GE39653
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 61kDa |
| OBSERVED MW: | 61kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 93 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT HALFTKHEDT LKRALAAIES RGYWSPFAEM PSPKVYGESG NADGEAAFKS HLGKTFELDQ PASGETVGAE RSPYGVALDI RYPKSTPDAL IAAAAAAQRT WREAGPSAWI GVSLEILARL NRASFEIAYS VMHTTGQAFM MAFQAGGPHA QDRALEAVAY AWDQLRRIPA DAHWEKPQGK XPPLAMQKRY TIVPRGTGLV LGCCTFPTXN GYPGLFADLA TGNTVIVKPH PGAILPLAIT VRIAARRAAR GPASILNVVT LLATEPXRRR TRAGPRACAP RSS*STSPGS TQNGTWLERH AHQAAGSTPR RQAVNQIVID STDDLKAAAK NIAFSLALYS GQMCTAPQNI YVPRDGIRTA DGHASFDEVA QAIAGAVQKL TGDPARSVEL IGAIQNDGVT ARIDAARAVG RVLLDSQTLQ HPAFPDARVR TPLVLQLDVA DREKFTQEWF GPISFVIATD STAQSLDLAG EIAAEHGALT LSVYSTADDV IDAAHEAAVR GGVALSINLT GGVFVNQSAA FSDFHGTGAN PAANAALADA AFVANRFRVV QSRVHVAPKA AXAEVGQPA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc catgcactgt tcacgaagca cgaagacacg ctgaaacgcg cactcgccgc catcgagagc cgcggatact ggagcccgtt cgccgaaatg ccgagcccca aagtgtacgg ggaaagcggc aatgcagacg gcgaagccgc gttcaaatcg cacctcggca agaccttcga gctcgaccag ccggccagcg gcgagacggt cggcgcggaa cgttctccgt atggcgtcgc gctcgacatc cggtatccga agtcgacgcc cgatgcgctg atcgcagccg ccgccgctgc ccaacgcacg tggcgcgaag ccggcccgag cgcgtggatc ggtgtcagcc tcgagatcct cgcgcgcctg aaccgcgcca gtttcgaaat cgcgtacagc gtgatgcata cgacgggcca ggcattcatg atggcgttcc aggccggcgg cccgcatgcg caggaccgcg cgctcgaggc cgtcgcctat gcgtgggacc agctgcgccg catcccggcc gacgcgcatt gggaaaagcc gcaaggcaag ancccgccgc tcgcgatgca gaagcgttac acgatcgtcc cgcgcggcac cgggctcgtg ctcggctgct gcacgttccc gacctgnaac ggttatcccg gcctgttcgc cgatctcgcg accggcaaca cggtcatcgt caagcctcat -ccgggcgcg atcctg-ccg ctcgcgatca ccgtgcggat cgcngcgcga cgtgctgcgc gaggsccggc ttcgatccta aacgtcgtga cgctgctcgc cacggagccs raacgacggc gcactcgt-g caggacctcg cgcntgcgcc cc-gagatca agctgatcga cttcaccggg cagcacgcag aacggcacgt gg-ctcgaac gtcacg-ccc atcaggcngc aggntctaca ccgagaaggc aggcngtcaa tcagatcgtg atcgactcga ccgacgatct gaaagccgcc gccaagaaca tcgcgttctc gctcgcgctg tactcggggc agatgtgcac cgccccgcag aacatctatg tgccgcgcga cggcatccgc accgccgacg gccatgcgag cttcgacgaa gtcgcgcagg cgatcgccgg cgcagtgcag aaactcaccg gcgacccggc acggtcggtc gagctgatcg gcgccatcca gaacgacggc gtgacggcac gcatcgacgc cgcgcgcgca gtcggccgcg tgctcctcga tagtcagacg cttcagcacc ccgccttccc cgacgctcgc gtgcgcacgc cgctcgtgct gcagctcgac gtcgccgacc gcgaaaaatt cacgcaggaa tggttcgggc cgatctcgtt cgtgatcgcg accgactcga ccgcgcagtc gctcgatctc gccggcgaga tcgccgccga gcacggcgcg ctgacgctgt ccgtctacag cacggccgac gacgtgatcg acgccgcaca cgaagccgcg gtgcgcggcg gcgtcgcact gtcgatcaac ctgacgggcg gcgtgttcgt caatcagtcg gcggcgttct ctgacttcca cggtacgggc gccaatccgg ccgcgaacgc tgcgctggcc gatgccgcat tcgtcgccaa ccgcttccgc gtggttcaga gccgcgtcca tgttgcgccg aaggcggcgc ncgcggaagt cggccaaccg gcanaa
Expected Protein Sequence
MAHHHHHHMT HALFTKHEDT LKRALAAIES RGYWSPFAEM PSPKVYGESG NADGEAAFKS HLGKTFELDQ PASGETVGAE RSPYGVALDI RYPKSTPDAL IAAAAAAQRT WREAGPSAWI GVSLEILARL NRASFEIAYS VMHTTGQAFM MAFQAGGPHA QDRALEAVAY AWDQLRRIPA DAHWEKPQGK NPPLAMQKRY TIVPRGTGLV LGCCTFPTWN GYPGLFADLA TGNTVIVKPH PGAILPLAIT VRIARDVLRE AGFDPNVVTL LATEPNDGAL VQDLALRPEI KLIDFTGSTQ NGTWLERHAH QAQVYTEKAG VNQIVIDSTD DLKAAAKNIA FSLALYSGQM CTAPQNIYVP RDGIRTADGH ASFDEVAQAI AGAVQKLTGD PARSVELIGA IQNDGVTARI DAARAVGRVL LDSQTLQHPA FPDARVRTPL VLQLDVADRE KFTQEWFGPI SFVIATDSTA QSLDLAGEIA AEHGALTLSV YSTADDVIDA AHEAAVRGGV ALSINLTGGV FVNQSAAFSD FHGTGANPAA NAALADAAFV ANRFRVVQSR VHVAPKAAPA EVGQPA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc atgcactgtt cacgaagcac gaagacacgc tgaaacgcgc actcgccgcc atcgagagcc gcggatactg gagcccgttc gccgaaatgc cgagccccaa agtgtacggg gaaagcggca atgcagacgg cgaagccgcg ttcaaatcgc acctcggcaa gaccttcgag ctcgaccagc cggccagcgg cgagacggtc ggcgcggaac gttctccgta tggcgtcgcg ctcgacatcc ggtatccgaa gtcgacgccc gatgcgctga tcgcagccgc cgccgctgcc caacgcacgt ggcgcgaagc cggcccgagc gcgtggatcg gtgtcagcct cgagatcctc gcgcgcctga accgcgccag tttcgaaatc gcgtacagcg tgatgcatac gacgggccag gcattcatga tggcgttcca ggccggcggc ccgcatgcgc aggaccgcgc gctcgaggcc gtcgcctatg cgtgggacca gctgcgccgc atcccggccg acgcgcattg ggaaaagccg caaggcaaga acccgccgct cgcgatgcag aagcgttaca cgatcgtccc gcgcggcacc gggctcgtgc tcggctgctg cacgttcccg acctggaacg gttatcccgg cctgttcgcc gatctcgcga ccggcaacac ggtcatcgtc aagcctcatc cgggcgcgat cctgccgctc gcgatcaccg tgcggatcgc gcgcgacgtg ctgcgcgagg ccggcttcga tcctaacgtc gtgacgctgc tcgccacgga gccgaacgac ggcgcactcg tgcaggacct cgcgctgcgc cccgagatca agctgatcga cttcaccggc agcacgcaga acggcacgtg gctcgaacgt cacgcccatc aggcgcaggt ctacaccgag aaggcaggcg tcaatcagat cgtgatcgac tcgaccgacg atctgaaagc cgccgccaag aacatcgcgt tctcgctcgc gctgtactcg gggcagatgt gcaccgcccc gcagaacatc tatgtgccgc gcgacggcat ccgcaccgcc gacggccatg cgagcttcga cgaagtcgcg caggcgatcg ccggcgcagt gcagaaactc accggcgacc cggcacggtc ggtcgagctg atcggcgcca tccagaacga cggcgtgacg gcacgcatcg acgccgcgcg cgcagtcggc cgcgtgctcc tcgatagtca gacgcttcag caccccgcct tccccgacgc tcgcgtgcgc acgccgctcg tgctgcagct cgacgtcgcc gaccgcgaaa aattcacgca ggaatggttc gggccgatct cgttcgtgat cgcgaccgac tcgaccgcgc agtcgctcga tctcgccggc gagatcgccg ccgagcacgg cgcgctgacg ctgtccgtct acagcacggc cgacgacgtg atcgacgccg cacacgaagc cgcggtgcgc ggcggcgtcg cactgtcgat caacctgacg ggcggcgtgt tcgtcaatca gtcggcggcg ttctctgact tccacggtac gggcgccaat ccggccgcga acgctgcgct ggccgatgcc gcattcgtcg ccaaccgctt ccgcgtggtt cagagccgcg tccatgttgc gccgaaggcg gcgcccgcgg aagtcggcca accggcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BumuA.00020.h.B1.PS37903
| PURIFICATION DATe: | 4/4/2016 |
| CONCENTRATION: | 39.9mg/ml |
| OBSERVED MW: | 61kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 93 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT HALFTKHEDT LKRALAAIES RGYWSPFAEM PSPKVYGESG NADGEAAFKS HLGKTFELDQ PASGETVGAE RSPYGVALDI RYPKSTPDAL IAAAAAAQRT WREAGPSAWI GVSLEILARL NRASFEIAYS VMHTTGQAFM MAFQAGGPHA QDRALEAVAY AWDQLRRIPA DAHWEKPQGK XPPLAMQKRY TIVPRGTGLV LGCCTFPTXN GYPGLFADLA TGNTVIVKPH PGAILPLAIT VRIAARRAAR GPASILNVVT LLATEPXRRR TRAGPRACAP RSS*STSPGS TQNGTWLERH AHQAAGSTPR RQAVNQIVID STDDLKAAAK NIAFSLALYS GQMCTAPQNI YVPRDGIRTA DGHASFDEVA QAIAGAVQKL TGDPARSVEL IGAIQNDGVT ARIDAARAVG RVLLDSQTLQ HPAFPDARVR TPLVLQLDVA DREKFTQEWF GPISFVIATD STAQSLDLAG EIAAEHGALT LSVYSTADDV IDAAHEAAVR GGVALSINLT GGVFVNQSAA FSDFHGTGAN PAANAALADA AFVANRFRVV QSRVHVAPKA AXAEVGQPA
Validated NT Sequence
atggctcacc accaccacca ccatatgacc catgcactgt tcacgaagca cgaagacacg ctgaaacgcg cactcgccgc catcgagagc cgcggatact ggagcccgtt cgccgaaatg ccgagcccca aagtgtacgg ggaaagcggc aatgcagacg gcgaagccgc gttcaaatcg cacctcggca agaccttcga gctcgaccag ccggccagcg gcgagacggt cggcgcggaa cgttctccgt atggcgtcgc gctcgacatc cggtatccga agtcgacgcc cgatgcgctg atcgcagccg ccgccgctgc ccaacgcacg tggcgcgaag ccggcccgag cgcgtggatc ggtgtcagcc tcgagatcct cgcgcgcctg aaccgcgcca gtttcgaaat cgcgtacagc gtgatgcata cgacgggcca ggcattcatg atggcgttcc aggccggcgg cccgcatgcg caggaccgcg cgctcgaggc cgtcgcctat gcgtgggacc agctgcgccg catcccggcc gacgcgcatt gggaaaagcc gcaaggcaag ancccgccgc tcgcgatgca gaagcgttac acgatcgtcc cgcgcggcac cgggctcgtg ctcggctgct gcacgttccc gacctgnaac ggttatcccg gcctgttcgc cgatctcgcg accggcaaca cggtcatcgt caagcctcat -ccgggcgcg atcctg-ccg ctcgcgatca ccgtgcggat cgcngcgcga cgtgctgcgc gaggsccggc ttcgatccta aacgtcgtga cgctgctcgc cacggagccs raacgacggc gcactcgt-g caggacctcg cgcntgcgcc cc-gagatca agctgatcga cttcaccggg cagcacgcag aacggcacgt gg-ctcgaac gtcacg-ccc atcaggcngc aggntctaca ccgagaaggc aggcngtcaa tcagatcgtg atcgactcga ccgacgatct gaaagccgcc gccaagaaca tcgcgttctc gctcgcgctg tactcggggc agatgtgcac cgccccgcag aacatctatg tgccgcgcga cggcatccgc accgccgacg gccatgcgag cttcgacgaa gtcgcgcagg cgatcgccgg cgcagtgcag aaactcaccg gcgacccggc acggtcggtc gagctgatcg gcgccatcca gaacgacggc gtgacggcac gcatcgacgc cgcgcgcgca gtcggccgcg tgctcctcga tagtcagacg cttcagcacc ccgccttccc cgacgctcgc gtgcgcacgc cgctcgtgct gcagctcgac gtcgccgacc gcgaaaaatt cacgcaggaa tggttcgggc cgatctcgtt cgtgatcgcg accgactcga ccgcgcagtc gctcgatctc gccggcgaga tcgccgccga gcacggcgcg ctgacgctgt ccgtctacag cacggccgac gacgtgatcg acgccgcaca cgaagccgcg gtgcgcggcg gcgtcgcact gtcgatcaac ctgacgggcg gcgtgttcgt caatcagtcg gcggcgttct ctgacttcca cggtacgggc gccaatccgg ccgcgaacgc tgcgctggcc gatgccgcat tcgtcgccaa ccgcttccgc gtggttcaga gccgcgtcca tgttgcgccg aaggcggcgc ncgcggaagt cggccaaccg gcanaa
Expressed Protein Sequence
MAHHHHHHMT HALFTKHEDT LKRALAAIES RGYWSPFAEM PSPKVYGESG NADGEAAFKS HLGKTFELDQ PASGETVGAE RSPYGVALDI RYPKSTPDAL IAAAAAAQRT WREAGPSAWI GVSLEILARL NRASFEIAYS VMHTTGQAFM MAFQAGGPHA QDRALEAVAY AWDQLRRIPA DAHWEKPQGK NPPLAMQKRY TIVPRGTGLV LGCCTFPTWN GYPGLFADLA TGNTVIVKPH PGAILPLAIT VRIARDVLRE AGFDPNVVTL LATEPNDGAL VQDLALRPEI KLIDFTGSTQ NGTWLERHAH QAQVYTEKAG VNQIVIDSTD DLKAAAKNIA FSLALYSGQM CTAPQNIYVP RDGIRTADGH ASFDEVAQAI AGAVQKLTGD PARSVELIGA IQNDGVTARI DAARAVGRVL LDSQTLQHPA FPDARVRTPL VLQLDVADRE KFTQEWFGPI SFVIATDSTA QSLDLAGEIA AEHGALTLSV YSTADDVIDA AHEAAVRGGV ALSINLTGGV FVNQSAAFSD FHGTGANPAA NAALADAAFV ANRFRVVQSR VHVAPKAAPA EVGQPA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC ATGCACTGTT CACGAAGCAC GAAGACACGC TGAAACGCGC ACTCGCCGCC ATCGAGAGCC GCGGATACTG GAGCCCGTTC GCCGAAATGC CGAGCCCCAA AGTGTACGGG GAAAGCGGCA ATGCAGACGG CGAAGCCGCG TTCAAATCGC ACCTCGGCAA GACCTTCGAG CTCGACCAGC CGGCCAGCGG CGAGACGGTC GGCGCGGAAC GTTCTCCGTA TGGCGTCGCG CTCGACATCC GGTATCCGAA GTCGACGCCC GATGCGCTGA TCGCAGCCGC CGCCGCTGCC CAACGCACGT GGCGCGAAGC CGGCCCGAGC GCGTGGATCG GTGTCAGCCT CGAGATCCTC GCGCGCCTGA ACCGCGCCAG TTTCGAAATC GCGTACAGCG TGATGCATAC GACGGGCCAG GCATTCATGA TGGCGTTCCA GGCCGGCGGC CCGCATGCGC AGGACCGCGC GCTCGAGGCC GTCGCCTATG CGTGGGACCA GCTGCGCCGC ATCCCGGCCG ACGCGCATTG GGAAAAGCCG CAAGGCAAGA ACCCGCCGCT CGCGATGCAG AAGCGTTACA CGATCGTCCC GCGCGGCACC GGGCTCGTGC TCGGCTGCTG CACGTTCCCG ACCTGGAACG GTTATCCCGG CCTGTTCGCC GATCTCGCGA CCGGCAACAC GGTCATCGTC AAGCCTCATC CGGGCGCGAT CCTGCCGCTC GCGATCACCG TGCGGATCGC GCGCGACGTG CTGCGCGAGG CCGGCTTCGA TCCTAACGTC GTGACGCTGC TCGCCACGGA GCCGAACGAC GGCGCACTCG TGCAGGACCT CGCGCTGCGC CCCGAGATCA AGCTGATCGA CTTCACCGGC AGCACGCAGA ACGGCACGTG GCTCGAACGT CACGCCCATC AGGCGCAGGT CTACACCGAG AAGGCAGGCG TCAATCAGAT CGTGATCGAC TCGACCGACG ATCTGAAAGC CGCCGCCAAG AACATCGCGT TCTCGCTCGC GCTGTACTCG GGGCAGATGT GCACCGCCCC GCAGAACATC TATGTGCCGC GCGACGGCAT CCGCACCGCC GACGGCCATG CGAGCTTCGA CGAAGTCGCG CAGGCGATCG CCGGCGCAGT GCAGAAACTC ACCGGCGACC CGGCACGGTC GGTCGAGCTG ATCGGCGCCA TCCAGAACGA CGGCGTGACG GCACGCATCG ACGCCGCGCG CGCAGTCGGC CGCGTGCTCC TCGATAGTCA GACGCTTCAG CACCCCGCCT TCCCCGACGC TCGCGTGCGC ACGCCGCTCG TGCTGCAGCT CGACGTCGCC GACCGCGAAA AATTCACGCA GGAATGGTTC GGGCCGATCT CGTTCGTGAT CGCGACCGAC TCGACCGCGC AGTCGCTCGA TCTCGCCGGC GAGATCGCCG CCGAGCACGG CGCGCTGACG CTGTCCGTCT ACAGCACGGC CGACGACGTG ATCGACGCCG CACACGAAGC CGCGGTGCGC GGCGGCGTCG CACTGTCGAT CAACCTGACG GGCGGCGTGT TCGTCAATCA GTCGGCGGCG TTCTCTGACT TCCACGGTAC GGGCGCCAAT CCGGCCGCGA ACGCTGCGCT GGCCGATGCC GCATTCGTCG CCAACCGCTT CCGCGTGGTT CAGAGCCGCG TCCATGTTGC GCCGAAGGCG GCGCCCGCGG AAGTCGGCCA ACCGGCATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT