BumuA.00010.z
Putative short-chain alcohol dehydrogenase
| CENTER ID: | BumuA.00010.z |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00010.z.B1.GE39650 | Full length( BumuA.00010.z ) | 1 | 237 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00010.z.B1.PS37887 | Full length( BumuA.00010.z ) | 1 | 237 |
Structures
5TT1
DEPOSITED: 10/31/2016
DETERMINATION: XRay
CLONE: BumuA.00010.z.B1.GE39650
PROTEIN: BumuA.00010.z.B1.PS37887
5UZX
DEPOSITED: 2/27/2017
DETERMINATION: XRay
CLONE: BumuA.00010.z.B1.GE39650
PROTEIN: BumuA.00010.z.B1.PS37887
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126860 |
| PATRIC ID: | fig|395019.8.peg.3803 |
| RefSeq: | YP_001583505.1 |
| UniProt: | A0A0H3KNM5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPDTSDPRPT ILLVGASRGL GHAMAAEFLK RGWDVVGTVR ADRGRTPLHA LAEAYPDRLR IETLDITQPE QIRALAARLS GRVFDILFVN AGTTNPDPTQ TIGEVSTDDF VDLMITNALS PMRVVETLAG LVPRDGLIGI MSSGQGSIAD NESGQRELYR GSKAALNQFM RSFAARHAQT PLAMVLIAPG WVRTELGGPD ARLSIDESVP GVVDVLLAKR GRAGLEYLDY RGRTVRW
NT Sequence
ATGCCCGATA CCTCTGACCC TCGTCCCACG ATCCTGCTCG TCGGCGCATC GCGCGGCCTC GGTCACGCAA TGGCGGCCGA GTTCCTGAAG CGCGGCTGGG ACGTCGTGGG CACCGTGCGC GCGGATCGCG GACGCACGCC GCTGCACGCG CTCGCCGAGG CGTACCCGGA CCGGCTGCGG ATCGAAACAC TCGATATCAC GCAGCCCGAG CAGATCCGCG CACTGGCCGC GCGCCTGTCC GGACGCGTGT TCGACATCCT GTTCGTCAAT GCCGGGACGA CGAATCCCGA TCCGACGCAG ACGATCGGCG AGGTGTCGAC CGACGATTTC GTCGACCTGA TGATCACGAA CGCACTGAGC CCGATGCGCG TGGTCGAGAC GCTGGCCGGC CTCGTGCCGC GCGACGGGCT GATCGGCATC ATGTCGTCGG GGCAGGGCAG CATCGCCGAC AACGAATCGG GCCAGCGCGA GCTCTATCGC GGCAGCAAGG CCGCGCTGAA CCAGTTCATG CGCAGCTTCG CGGCGCGTCA CGCGCAGACG CCGCTCGCGA TGGTGCTGAT CGCGCCCGGC TGGGTGCGCA CGGAGCTCGG CGGGCCGGAT GCGCGGCTGT CGATCGACGA AAGCGTGCCG GGCGTCGTCG ATGTGCTGCT CGCCAAGCGC GGCCGCGCGG GGCTCGAATA TCTCGACTAT CGCGGTCGAA CCGTGCGGTG G
Details for BumuA.00010.z.B1.GE39650
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMP DTSDPRPTIL LVGASRGLGH AMAAEFLKRG WDVVGTVRAD RGRTPLHALA EAYPDRLRIE TLDITQPEQI RALAARLSGR VFDILFVNAG TTNPDPTQTI GEVSTDDFVD LMITNALSPM RVVETLAGLV PRDGLIGIMS SGQGSIADNE SGQRELYRGS KAALNQFMRS FAARHAQTPL AMVLIAPGWV RTELGGPDAR LSIDESVPGV VDVLLAKRGR AGLEYLDYRG RTVRW
Validated NT Sequence
atcttactca cttaccaccg cacggttcga ccgcgatagt cgagatattc gagccccgcg cggccgcgct tggcgagcag cacatcgacg acgcccggca cgctttcgtc gatcgacagc cgcgcatccg gcccgccgag ctccgtgcgc acccagccgg gcgcgatcag caccatcgcg agcggcgtct gcgcgtgacg cgccgcgaag ctgcgcatga actggttcag cgcggccttg ctgccgcgat agagctcgcg ctggcccgat tcgttgtcgg cgatgctgcc ctgccccgac gacatgatgc cgatcagccc gtcgcgcggc acgaggccgg ccagcgtctc gaccacgcgc atcgggctca gtgcgttcgt gatcatcagg tcgacgaaat cgtcggtcga cacctcgccg atcgtctgcg tcggatcggg attcgtcgtc ccggcattga cgaacaggat gtcgaacacg cgtccggaca ggcgcgcggc cagtgcgcgg atctgctcgg gctgcgtgat atcgagtgtt tcgatccgca gccggtccgg gtacgcctcg gcgagcgcgt gcagcggcgt gcgtccgcga tccgcgcgca cggtgcccac gacgtcccag ccgcgcttca ggaactcggc cgccattgcg tgaccgaggc cgcgcgatgc gccgacgagc aggatcgtgg gacgagggtc agaggtatcg ggcatatggt ggtggtggtg gtgagccatg g
Expected Protein Sequence
MAHHHHHHMP DTSDPRPTIL LVGASRGLGH AMAAEFLKRG WDVVGTVRAD RGRTPLHALA EAYPDRLRIE TLDITQPEQI RALAARLSGR VFDILFVNAG TTNPDPTQTI GEVSTDDFVD LMITNALSPM RVVETLAGLV PRDGLIGIMS SGQGSIADNE SGQRELYRGS KAALNQFMRS FAARHAQTPL AMVLIAPGWV RTELGGPDAR LSIDESVPGV VDVLLAKRGR AGLEYLDYRG RTVRW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg atacctctga ccctcgtccc acgatcctgc tcgtcggcgc atcgcgcggc ctcggtcacg caatggcggc cgagttcctg aagcgcggct gggacgtcgt gggcaccgtg cgcgcggatc gcggacgcac gccgctgcac gcgctcgccg aggcgtaccc ggaccggctg cggatcgaaa cactcgatat cacgcagccc gagcagatcc gcgcactggc cgcgcgcctg tccggacgcg tgttcgacat cctgttcgtc aatgccggga cgacgaatcc cgatccgacg cagacgatcg gcgaggtgtc gaccgacgat ttcgtcgacc tgatgatcac gaacgcactg agcccgatgc gcgtggtcga gacgctggcc ggcctcgtgc cgcgcgacgg gctgatcggc atcatgtcgt cggggcaggg cagcatcgcc gacaacgaat cgggccagcg cgagctctat cgcggcagca aggccgcgct gaaccagttc atgcgcagct tcgcggcgcg tcacgcgcag acgccgctcg cgatggtgct gatcgcgccc ggctgggtgc gcacggagct cggcgggccg gatgcgcggc tgtcgatcga cgaaagcgtg ccgggcgtcg tcgatgtgct gctcgccaag cgcggccgcg cggggctcga atatctcgac tatcgcggtc gaaccgtgcg gtggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BumuA.00010.z.B1.PS37887
| PURIFICATION DATe: | 3/24/2016 |
| CONCENTRATION: | 50mg/ml |
| OBSERVED MW: | 25kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP DTSDPRPTIL LVGASRGLGH AMAAEFLKRG WDVVGTVRAD RGRTPLHALA EAYPDRLRIE TLDITQPEQI RALAARLSGR VFDILFVNAG TTNPDPTQTI GEVSTDDFVD LMITNALSPM RVVETLAGLV PRDGLIGIMS SGQGSIADNE SGQRELYRGS KAALNQFMRS FAARHAQTPL AMVLIAPGWV RTELGGPDAR LSIDESVPGV VDVLLAKRGR AGLEYLDYRG RTVRW
Validated NT Sequence
atcttactca cttaccaccg cacggttcga ccgcgatagt cgagatattc gagccccgcg cggccgcgct tggcgagcag cacatcgacg acgcccggca cgctttcgtc gatcgacagc cgcgcatccg gcccgccgag ctccgtgcgc acccagccgg gcgcgatcag caccatcgcg agcggcgtct gcgcgtgacg cgccgcgaag ctgcgcatga actggttcag cgcggccttg ctgccgcgat agagctcgcg ctggcccgat tcgttgtcgg cgatgctgcc ctgccccgac gacatgatgc cgatcagccc gtcgcgcggc acgaggccgg ccagcgtctc gaccacgcgc atcgggctca gtgcgttcgt gatcatcagg tcgacgaaat cgtcggtcga cacctcgccg atcgtctgcg tcggatcggg attcgtcgtc ccggcattga cgaacaggat gtcgaacacg cgtccggaca ggcgcgcggc cagtgcgcgg atctgctcgg gctgcgtgat atcgagtgtt tcgatccgca gccggtccgg gtacgcctcg gcgagcgcgt gcagcggcgt gcgtccgcga tccgcgcgca cggtgcccac gacgtcccag ccgcgcttca ggaactcggc cgccattgcg tgaccgaggc cgcgcgatgc gccgacgagc aggatcgtgg gacgagggtc agaggtatcg ggcatatggt ggtggtggtg gtgagccatg g
Expressed Protein Sequence
MAHHHHHHMP DTSDPRPTIL LVGASRGLGH AMAAEFLKRG WDVVGTVRAD RGRTPLHALA EAYPDRLRIE TLDITQPEQI RALAARLSGR VFDILFVNAG TTNPDPTQTI GEVSTDDFVD LMITNALSPM RVVETLAGLV PRDGLIGIMS SGQGSIADNE SGQRELYRGS KAALNQFMRS FAARHAQTPL AMVLIAPGWV RTELGGPDAR LSIDESVPGV VDVLLAKRGR AGLEYLDYRG RTVRW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG ATACCTCTGA CCCTCGTCCC ACGATCCTGC TCGTCGGCGC ATCGCGCGGC CTCGGTCACG CAATGGCGGC CGAGTTCCTG AAGCGCGGCT GGGACGTCGT GGGCACCGTG CGCGCGGATC GCGGACGCAC GCCGCTGCAC GCGCTCGCCG AGGCGTACCC GGACCGGCTG CGGATCGAAA CACTCGATAT CACGCAGCCC GAGCAGATCC GCGCACTGGC CGCGCGCCTG TCCGGACGCG TGTTCGACAT CCTGTTCGTC AATGCCGGGA CGACGAATCC CGATCCGACG CAGACGATCG GCGAGGTGTC GACCGACGAT TTCGTCGACC TGATGATCAC GAACGCACTG AGCCCGATGC GCGTGGTCGA GACGCTGGCC GGCCTCGTGC CGCGCGACGG GCTGATCGGC ATCATGTCGT CGGGGCAGGG CAGCATCGCC GACAACGAAT CGGGCCAGCG CGAGCTCTAT CGCGGCAGCA AGGCCGCGCT GAACCAGTTC ATGCGCAGCT TCGCGGCGCG TCACGCGCAG ACGCCGCTCG CGATGGTGCT GATCGCGCCC GGCTGGGTGC GCACGGAGCT CGGCGGGCCG GATGCGCGGC TGTCGATCGA CGAAAGCGTG CCGGGCGTCG TCGATGTGCT GCTCGCCAAG CGCGGCCGCG CGGGGCTCGA ATATCTCGAC TATCGCGGTC GAACCGTGCG GTGGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT