BumuA.00010.u
Putative short-chain alcohol dehydrogenase
| CENTER ID: | BumuA.00010.u |
| ORGANISM: | Burkholderia multivorans ATCC 17616 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00010.u.B1.GE39648 | Full length( BumuA.00010.u ) | 1 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumuA.00010.u.B1.PS37896 | Full length( BumuA.00010.u ) | 1 | 265 |
Structures
5UNL
DEPOSITED: 1/31/2017
DETERMINATION: XRay
CLONE: BumuA.00010.u.B1.GE39648
PROTEIN: BumuA.00010.u.B1.PS37896
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|395019.8.peg.4596 |
| RefSeq: | YP_001584249.1 |
| UniProt: | A0A0H3KLG6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTASDLLKPY DGLRVLVTGG ASGIGLAIAD AFAECGARVH VCDASQAAIA ALADRPSRAA IGATLADVSD RAAVERVFAD VAATLGGLDV LVNNAGIAGP TGGIDEIDPA QWEQTVAINL NAQFEFARRA VPLLRESKHG GAIIALSSVA GRLGYAYRTP YAATKWAVVG LVKSLAIELG PLGIRVNAIQ PGIVRGPRIR RVIEARAQQL GIGYDEMEQR YLERISLRRM TEPAEIAATA LFLCSPGGHG ITGQAISVCG NVEVL
NT Sequence
ATGACCGCTT CCGACCTGCT GAAACCCTAT GACGGCCTTC GCGTCCTCGT GACCGGCGGC GCATCCGGCA TCGGCCTCGC CATCGCCGAT GCGTTCGCCG AATGCGGTGC GCGCGTGCAT GTCTGCGACG CGTCGCAGGC CGCGATCGCG GCGCTCGCCG ACCGGCCGTC GCGTGCCGCG ATCGGCGCAA CGCTTGCCGA CGTATCGGAC CGCGCGGCGG TCGAGCGCGT GTTCGCGGAC GTCGCGGCAA CGCTCGGCGG CCTCGACGTA CTGGTCAACA ACGCGGGCAT CGCGGGCCCG ACCGGCGGCA TCGACGAGAT CGATCCCGCG CAGTGGGAGC AGACGGTCGC GATCAATCTG AACGCGCAAT TCGAATTCGC GCGACGCGCT GTGCCGCTGC TGCGCGAGTC GAAGCACGGT GGCGCCATCA TCGCGCTGTC GTCGGTCGCG GGCCGGCTCG GCTATGCGTA CCGCACGCCT TACGCGGCCA CCAAATGGGC GGTCGTCGGT CTCGTGAAGA GCCTCGCGAT CGAACTGGGG CCGCTCGGCA TTCGCGTGAA TGCGATCCAG CCCGGCATCG TGCGCGGCCC GCGCATCCGC CGCGTGATCG AAGCGCGCGC GCAACAGCTC GGCATCGGCT ACGACGAGAT GGAGCAGCGC TATCTCGAAC GGATTTCGCT GCGCCGGATG ACCGAGCCGG CCGAAATCGC GGCGACCGCG CTGTTCCTGT GCTCGCCCGG CGGGCACGGC ATCACCGGCC AGGCGATCTC CGTCTGCGGC AACGTCGAAG TGCTC
Details for BumuA.00010.u.B1.GE39648
| HARVESTED ON: | 12/3/2015 |
| SEQUENCED ON: | 12/7/2015 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MXASDLLKPY DGLRVLVTGG ASGIGLAIAD AFAECGARVH VCDASQAAIA ALADRPSRAA IGATLADVSD RAAVERVFAD VAATLGGLDV LVNNAGIAGP TGGIDEIDPA QWEQTVAINL NAQFEFARRA VPLLRESKHG GAIIALSSVA GRLGYAYRTP YAATKWAVVG LVKSLAIELG PLGIRVNAIQ PGIVRGPRIR RVIEARAQQL GIGYDEMEQR YLERISLRRM TEPAEIAATA LFLCSPGGHG ITGQAISVCG NVEVL
Validated NT Sequence
ggctcaccac caccaccacc atatgntcgc ttccgacctg ctgaaaccct atgacggcct tcgcgtcctc gtgaccggcg gcgcatccgg catcggcctc gccatcgccg atgcgttcgc cgaatgcggt gcgcgcgtgc atgtctgcga cgcgtcgcag gccgcgatcg cggcgctcgc cgaccggccg tcgcgtgccg cgatcggcgc aacgcttgcc gacgtatcgg accgcgcggc ggtcgagcgc gtgttcgcgg acgtcgcggc aacgctcggc ggcctcgacg tactggtcaa caacgcgggc atcgcgggcc cgaccggcgg catcgacgag atcgatcccg cgcagtggga gcagacggtc gcgatcaatc tgaacgcgca attcgaattc gcgcgacgcg ctgtgccgct gctgcgcgag tcgaagcacg gtggcgccat catcgcgctg tcgtcggtcg cgggccggct cggctatgcg taccgcacgc cttacgcggc caccaaatgg gcggtcgtcg gtctcgtgaa gagcctcgcg atcgaactgg ggccgctcgg cattcgcgtg aatgcgatcc agcccggcat cgtgcgcggc ccgcgcatcc gccgcgtgat cgaagcgcgc gcgcaacagc tcggcatcgg ctacgacgag atggagcagc gctatctcga acggatttcg ctgcgccgga tgaccgagcc ggccgaaatc gcggcgaccg cgctgttcct gtgctcgccc ggcgggcacg gcatcaccgg ccaggcgatc tccgtctgcg gcaacgtcga agtgctctaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMT ASDLLKPYDG LRVLVTGGAS GIGLAIADAF AECGARVHVC DASQAAIAAL ADRPSRAAIG ATLADVSDRA AVERVFADVA ATLGGLDVLV NNAGIAGPTG GIDEIDPAQW EQTVAINLNA QFEFARRAVP LLRESKHGGA IIALSSVAGR LGYAYRTPYA ATKWAVVGLV KSLAIELGPL GIRVNAIQPG IVRGPRIRRV IEARAQQLGI GYDEMEQRYL ERISLRRMTE PAEIAATALF LCSPGGHGIT GQAISVCGNV EVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg cttccgacct gctgaaaccc tatgacggcc ttcgcgtcct cgtgaccggc ggcgcatccg gcatcggcct cgccatcgcc gatgcgttcg ccgaatgcgg tgcgcgcgtg catgtctgcg acgcgtcgca ggccgcgatc gcggcgctcg ccgaccggcc gtcgcgtgcc gcgatcggcg caacgcttgc cgacgtatcg gaccgcgcgg cggtcgagcg cgtgttcgcg gacgtcgcgg caacgctcgg cggcctcgac gtactggtca acaacgcggg catcgcgggc ccgaccggcg gcatcgacga gatcgatccc gcgcagtggg agcagacggt cgcgatcaat ctgaacgcgc aattcgaatt cgcgcgacgc gctgtgccgc tgctgcgcga gtcgaagcac ggtggcgcca tcatcgcgct gtcgtcggtc gcgggccggc tcggctatgc gtaccgcacg ccttacgcgg ccaccaaatg ggcggtcgtc ggtctcgtga agagcctcgc gatcgaactg gggccgctcg gcattcgcgt gaatgcgatc cagcccggca tcgtgcgcgg cccgcgcatc cgccgcgtga tcgaagcgcg cgcgcaacag ctcggcatcg gctacgacga gatggagcag cgctatctcg aacggatttc gctgcgccgg atgaccgagc cggccgaaat cgcggcgacc gcgctgttcc tgtgctcgcc cggcgggcac ggcatcaccg gccaggcgat ctccgtctgc ggcaacgtcg aagtgctctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BumuA.00010.u.B1.PS37896
| PURIFICATION DATe: | 3/30/2016 |
| CONCENTRATION: | 36mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MXASDLLKPY DGLRVLVTGG ASGIGLAIAD AFAECGARVH VCDASQAAIA ALADRPSRAA IGATLADVSD RAAVERVFAD VAATLGGLDV LVNNAGIAGP TGGIDEIDPA QWEQTVAINL NAQFEFARRA VPLLRESKHG GAIIALSSVA GRLGYAYRTP YAATKWAVVG LVKSLAIELG PLGIRVNAIQ PGIVRGPRIR RVIEARAQQL GIGYDEMEQR YLERISLRRM TEPAEIAATA LFLCSPGGHG ITGQAISVCG NVEVL
Validated NT Sequence
ggctcaccac caccaccacc atatgntcgc ttccgacctg ctgaaaccct atgacggcct tcgcgtcctc gtgaccggcg gcgcatccgg catcggcctc gccatcgccg atgcgttcgc cgaatgcggt gcgcgcgtgc atgtctgcga cgcgtcgcag gccgcgatcg cggcgctcgc cgaccggccg tcgcgtgccg cgatcggcgc aacgcttgcc gacgtatcgg accgcgcggc ggtcgagcgc gtgttcgcgg acgtcgcggc aacgctcggc ggcctcgacg tactggtcaa caacgcgggc atcgcgggcc cgaccggcgg catcgacgag atcgatcccg cgcagtggga gcagacggtc gcgatcaatc tgaacgcgca attcgaattc gcgcgacgcg ctgtgccgct gctgcgcgag tcgaagcacg gtggcgccat catcgcgctg tcgtcggtcg cgggccggct cggctatgcg taccgcacgc cttacgcggc caccaaatgg gcggtcgtcg gtctcgtgaa gagcctcgcg atcgaactgg ggccgctcgg cattcgcgtg aatgcgatcc agcccggcat cgtgcgcggc ccgcgcatcc gccgcgtgat cgaagcgcgc gcgcaacagc tcggcatcgg ctacgacgag atggagcagc gctatctcga acggatttcg ctgcgccgga tgaccgagcc ggccgaaatc gcggcgaccg cgctgttcct gtgctcgccc ggcgggcacg gcatcaccgg ccaggcgatc tccgtctgcg gcaacgtcga agtgctctaa gtgagtaaga t
Expressed Protein Sequence
MAHHHHHHMT ASDLLKPYDG LRVLVTGGAS GIGLAIADAF AECGARVHVC DASQAAIAAL ADRPSRAAIG ATLADVSDRA AVERVFADVA ATLGGLDVLV NNAGIAGPTG GIDEIDPAQW EQTVAINLNA QFEFARRAVP LLRESKHGGA IIALSSVAGR LGYAYRTPYA ATKWAVVGLV KSLAIELGPL GIRVNAIQPG IVRGPRIRRV IEARAQQLGI GYDEMEQRYL ERISLRRMTE PAEIAATALF LCSPGGHGIT GQAISVCGNV EVL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CTTCCGACCT GCTGAAACCC TATGACGGCC TTCGCGTCCT CGTGACCGGC GGCGCATCCG GCATCGGCCT CGCCATCGCC GATGCGTTCG CCGAATGCGG TGCGCGCGTG CATGTCTGCG ACGCGTCGCA GGCCGCGATC GCGGCGCTCG CCGACCGGCC GTCGCGTGCC GCGATCGGCG CAACGCTTGC CGACGTATCG GACCGCGCGG CGGTCGAGCG CGTGTTCGCG GACGTCGCGG CAACGCTCGG CGGCCTCGAC GTACTGGTCA ACAACGCGGG CATCGCGGGC CCGACCGGCG GCATCGACGA GATCGATCCC GCGCAGTGGG AGCAGACGGT CGCGATCAAT CTGAACGCGC AATTCGAATT CGCGCGACGC GCTGTGCCGC TGCTGCGCGA GTCGAAGCAC GGTGGCGCCA TCATCGCGCT GTCGTCGGTC GCGGGCCGGC TCGGCTATGC GTACCGCACG CCTTACGCGG CCACCAAATG GGCGGTCGTC GGTCTCGTGA AGAGCCTCGC GATCGAACTG GGGCCGCTCG GCATTCGCGT GAATGCGATC CAGCCCGGCA TCGTGCGCGG CCCGCGCATC CGCCGCGTGA TCGAAGCGCG CGCGCAACAG CTCGGCATCG GCTACGACGA GATGGAGCAG CGCTATCTCG AACGGATTTC GCTGCGCCGG ATGACCGAGC CGGCCGAAAT CGCGGCGACC GCGCTGTTCC TGTGCTCGCC CGGCGGGCAC GGCATCACCG GCCAGGCGAT CTCCGTCTGC GGCAACGTCG AAGTGCTCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT