SSGCID: Target Page

BumaA.19929.a

T6SS-2 membrane complex protein (BMAA0454)

CENTER ID:	BumaA.19929.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.19929.a.B2.GE42219	ImcF-related and ImcF domains		467	1147

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.19929.a.B2.PW38470	ImcF-related and ImcF domains		467	1147

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.3955
UniProt:	A0A0H2WC13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGPLFAFGIY HPLGPVWVRE ILVALVLIWG FWPTLARLWA RLAMSPRQVK VAPKAKQLDF VDKHLRTLDQ QLKERWRKEP RGRWKRWVGA LTREHRTMLP WYLVLGSEGS GKSSLVAKAV SVSGSLQDRV LGSDATYGRG DDLNFRITRE AVWFDVGGRW SLRAGADEAE FDAWRKLLRG MRRLRRGAPI SGVVLCVDGL EMIDAPLDAR KRLADSVRAR LEEMREAFGQ QVQVYVALNG LDRLDGAVST LSLLEASKWV KGVGFSLPDD GAHADAARAD ASWQHALQGL QQRVQQQVLY SAPAATEVSM NHAQLRFVET LSRLQKGLVA WLHVALAPGE PHTAARLRGV WLGSMAELAE PHPAGAGGVG SAELPVPSRP LSELWTPLIR QVALERDAVR PSGPKSWRGR LGEALRWGAV PLVALSLLLW FGWGYVTERD YLDGVWAQFT EAKRLAQAEA SYGNDGGSTL IEIANQMRYA QLQAEDAAQG MATPYFEHGL VAETARETYY RHLQKMLMPE LYNEVRRTLV SQVDGSPGDI YQTLKVYLML CRPARRSADD VVRWLDGRWD ALSGGQYSDD DRRSLLAHVR TLMSLKEVPA TPEDANLVRS ARAKAAQIPL VTRVLQHIHA QGLPQQVNDI SLSRAAGFEA SMSLRMRSNV PSTDTAVSGW FTRAGYTDVF LPRLQKSARA MLEEESWVLR DETLSGNSFQ IDGLVQKLAD SARNQFLQDY ISAWQNFLND VTVRGVTGLD DASQLAAAMM EAQSPLANLL RFAARETTLT GASDEGNIDS WIDRQKYRFE KGRRQVVGEL SGQHYRTVLL PEHVVEEHFQ AIRQLAAQLN RNNTIANNPL SRLFEPLYRQ LGLVNGALQA GQVLPAQYDA FSRLKETAAR QPEPVRGIML DLVSSGSTMT TRESGALLNR GAAGATKMVC DQGFTGRYPM RRGAQADAGV EDFERLFSAQ GLMATYFRDH LAAYVDTSAK PWQALRSNGG PNGMVSQSVL NSYETAERIR GAMLDDSGHL RVSTVLRFID MDSQLSEAQL SVAGQTVRFA HGVTSPHRVD WTNQNTQLAI KLQLKSVDGR MTTLQFDGPW ALFRFFDAGQ AVGGTGTADR RERLYQTSLG TVRIEWQALT LPSPIWSGIL QSFRCPS

NT Sequence

gtggggccgc tgttcgcgtt cggcatctat cacccgctcg gcccggtgtg ggtgcgcgag atcctcgtcg cactggtgct gatctggggc ttctggccga cgctcgcgcg gctctgggcg cggctcgcga tgagcccgcg gcaggtgaag gtcgcgccga aggccaagca gctcgatttc gtcgacaagc atctgcgcac cctcgaccag cagttgaagg agcgctggcg caaggagccg cgcggccgct ggaagcgctg ggtcggcgcg ctcacgcgcg agcatcgcac gatgctgccg tggtatctgg tgctcggctc cgagggtagc ggcaagtcga gcctcgtcgc gaaggcggtg agcgtgtccg gctcgctgca ggaccgggtg ctcggctcgg acgcgaccta cgggcgcggc gacgacttga acttccgcat cacgcgcgag gcggtgtggt tcgacgtcgg cggccgctgg agcctgcgcg cgggtgccga cgaggccgag ttcgacgcgt ggcgcaagct gctgcgcggg atgaggcggc tgcgcagggg cgcgccgatc agcggcgtcg tgctgtgcgt cgacggcctc gagatgatcg atgcgccgct cgacgcgcgc aagcggctgg ccgattcggt gcgcgcgcgg ctcgaggaga tgcgcgaggc gttcggccag caggtgcagg tgtacgttgc gctgaacggg ctcgaccggc tcgacggcgc ggtctcgacg ctgtcgctgc tcgaggcgtc gaagtgggtc aagggcgtgg gcttcagcct gcccgacgac ggcgcgcacg ccgacgccgc gcgcgcggac gcgagctggc agcacgcgct gcaagggctg cagcagcggg tccagcagca ggtgctgtat tcggcgccgg cggcgaccga ggtgtcgatg aaccacgcgc agttgcgctt cgtcgagacg ctcagccgcc tgcagaaggg gctcgtcgcg tggctgcacg tcgcgctcgc gccgggcgag ccgcacacgg ccgcgcggct gcgcggggtg tggctgggct cgatggccga gctcgcggag ccgcatccgg cgggcgcggg cggcgtcggc tcggcggagc tgcccgtgcc gtcgcgcccg ctcagcgagc tgtggacgcc gctcatccgg caggtggcgc tcgagcgcga cgcggtgcgc ccgagcgggc cgaagtcgtg gcgcgggcgc ctgggcgagg cgctgcgctg gggcgcggtg cccctcgtcg cgttgagcct gctgctgtgg ttcggctggg gctacgtcac cgagcgcgac tatctggacg gcgtctgggc gcagttcacc gaggccaagc ggctcgcgca ggccgaggcg tcctacggca acgacggcgg ctcgacgctc atcgagatcg cgaaccagat gcgctatgcg caactgcagg ccgaggacgc cgcgcagggc atggcgacgc cgtacttcga gcacgggctc gtcgcggaga ccgcgcgcga gacctactac cggcacttgc agaagatgct gatgccggag ttgtacaacg aggtgcggcg cacgctcgtg tcgcaggtcg acggcagccc gggcgacatc taccagacgc tgaaggtcta cctgatgctc tgccgccccg cgcggcgctc ggcggacgac gtcgtgcgct ggctcgacgg ccgctgggac gcgctctcgg gcgggcagta ctcggacgac gaccgccgct cgctgctcgc gcacgtgcgc acgctgatgt cgctgaagga ggtgccggcc acgccggagg acgcgaacct cgtgcgctcg gcgcgcgcga aggccgcgca gatcccgctc gtcacgcgcg tgctgcagca catccacgcg caggggctgc cgcagcaggt caacgacatc tcgctgtcgc gcgcggcggg cttcgaggcg tcgatgagcc tgcgcatgcg cagcaacgtg ccgtcgaccg acaccgcggt gtcgggctgg ttcacgcgcg cgggctacac ggacgtgttc ctgccgcgcc tgcagaagag cgcgcgcgcg atgctcgagg aggagagctg ggtgctgcgc gacgagacgc tgtcgggcaa cagcttccag atcgacggac tcgtgcagaa gctcgccgat tcggcgcgca accagttcct gcaggactac atctccgcgt ggcagaactt cctgaacgac gtgacggtgc gcggcgtgac gggcctggac gatgcgtcgc agctcgccgc ggcgatgatg gaggcgcaat cgccgctcgc gaacctgctg cgcttcgccg cgcgcgagac gacgctcacg ggcgcgagcg acgaaggcaa catcgacagc tggatcgatc gccagaagta ccgcttcgag aaggggcggc ggcaggtcgt cggcgagctg agcgggcagc attaccgcac ggtgctgctg ccggagcacg tggtcgagga gcacttccag gcgatccgcc agctcgccgc gcaactgaac cgcaacaaca cgatcgcgaa caacccgctg tcgcggctgt tcgagccgct gtaccggcag ctcgggctcg tcaacggcgc gctgcaggcg ggccaggtgc tgcccgcgca gtacgacgcg ttctcgcggc tgaaggagac ggccgcgcgc cagccggagc cggtgcgcgg gatcatgctc gacctcgtga gcagcggcag cacgatgacg acgcgcgaat cgggcgcgct gctcaaccgg ggcgcggcgg gcgcgacgaa gatggtctgc gatcagggct tcacgggccg ctatccgatg cgccgcggcg cgcaggcgga tgcgggcgtg gaggacttcg agcggctgtt cagcgcgcag gggctgatgg cgacgtactt ccgcgatcac ctcgccgcgt atgtggacac gtcggccaag ccgtggcagg cgctgcgctc gaacggcggg ccgaacggga tggtgagcca gtcggtgctg aactcgtacg agacggccga gcgcatccgc ggcgcgatgc tcgacgattc ggggcacctg cgcgtgtcga ccgtgctgcg tttcatcgac atggattcgc agttgtccga agcgcagctg agcgtggctg ggcagacggt gcgcttcgcg cacggcgtga cctcgccgca ccgggtggac tggaccaacc agaacacgca gctcgcgatc aagctgcagc tcaaatcggt ggacggccgg atgacgacgc tgcagttcga cgggccgtgg gcgctgttcc gcttcttcga cgcggggcag gcggtgggcg gcacgggcac cgcggaccgg cgcgagcggc tgtatcagac gagcctcggc acggtgcgca tcgaatggca ggcgctcacg ctgccgtcgc cgatatggtc gggcatcctg cagtcgttca ggtgtccgtc gtga

Details for BumaA.19929.a.B2.GE42219

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	77kDa
OBSERVED MW:	75kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHGS TLIEIANQMR YAQLQAEDAA QGMATPYFEH GLVAETARET YYRHLQKMLM PELYNEVRRT LVSQVDGSPG DIYQTLKVYL MLCRPARRSA DDVVRWLDGR WDALSGGQYS DDDRRSLLAH VRTLMSLKEV PATPEDANLV RSARAKAAQI PLVTRVLQHI HAQGLPQQVN DISLSRAAGF EASMSLRMRS NVPSTDTAVS GWFTRAGYTD VFLPRLQKSA RAMLEEESWV LRDETLSGNS FQIDGLVQKL ADSARNQFLQ DYISAWQNFL NDVTVRGVTG LDDASQLAAA MMEAQSPLAN LLRFAARETT LTGASDEGNI DSWIDRQKYR FEKGRRQVVG ELSGQHYRTV LLPEHVVEEH FQAIRQLAAQ LNRNNTIANN PLSRLFEPLY RQLGLVNGAL QAGQVLPAQY DAFSRLKETA ARQPEPVRGI MLDLVSSGST MTTRESGALL NRGAAGATKM VCDQGFTGRY PMRRGAQADA GVEDFERLFS AQGLMATYFR DHLAAYVDTS AKPWQALRSN GGPNGMVSQS VLNSYETAER IRGAMLDDSG HLRVSTVLRF IDMDSQLSEA QLSVAGQTVR FAHGVTSPHR VDWTNQNTQL AIKLQLKSVD GRMTTLQFDG PWALFRFFDA GQAVGGTGTA DRRERLYQTS LGTVRIEWQA LTLPSPIWSG ILQSFRCPS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggctcga cgctcatcga gatcgcgaac cagatgcgct atgcgcaact gcaggccgag gacgccgcgc agggcatggc gacgccgtac ttcgagcacg ggctcgtcgc ggagaccgcg cgcgagacct actaccggca cttgcagaag atgctgatgc cggagttgta caacgaggtg cggcgcacgc tcgtgtcgca ggtcgacggc agcccgggcg acatctacca gacgctgaag gtctacctga tgctctgccg ccccgcgcgg cgctcggcgg acgacgtcgt gcgctggctc gacggccgct gggacgcgct ctcgggcggg cagtactcgg acgacgaccg ccgctcgctg ctcgcgcacg tgcgcacgct gatgtcgctg aaggaggtgc cggccacgcc ggaggacgcg aacctcgtgc gctcggcgcg cgcgaaggcc gcgcagatcc cgctcgtcac gcgcgtgctg cagcacatcc acgcgcaggg gctgccgcag caggtcaacg acatctcgct gtcgcgcgcg gcgggcttcg aggcgtcgat gagcctgcgc atgcgcagca acgtgccgtc gaccgacacc gcggtgtcgg gctggttcac gcgcgcgggc tacacggacg tgttcctgcc gcgcctgcag aagagcgcgc gcgcgatgct cgaggaggag agctgggtgc tgcgcgacga gacgctgtcg ggcaacagct tccagatcga cggactcgtg cagaagctcg ccgattcggc gcgcaaccag ttcctgcagg actacatctc cgcgtggcag aacttcctga acgacgtgac ggtgcgcggc gtgacgggcc tggacgatgc gtcgcagctc gccgcggcga tgatggaggc gcaatcgccg ctcgcgaacc tgctgcgctt cgccgcgcgc gagacgacgc tcacgggcgc gagcgacgaa ggcaacatcg acagctggat cgatcgccag aagtaccgct tcgagaaggg gcggcggcag gtcgtcggcg agctgagcgg gcagcattac cgcacggtgc tgctgccgga gcacgtggtc gaggagcact tccaggcgat ccgccagctc gccgcgcaac tgaaccgcaa caacacgatc gcgaacaacc cgctgtcgcg gctgttcgag ccgctgtacc ggcagctcgg gctcgtcaac ggcgcgctgc aggcgggcca ggtgctgccc gcgcagtacg acgcgttctc gcggctgaag gagacggccg cgcgccagcc ggagccggtg cgcgggatca tgctcgacct cgtgagcagc ggcagcacga tgacgacgcg cgaatcgggc gcgctgctca accggggcgc ggcgggcgcg acgaagatgg tctgcgatca gggcttcacg ggccgctatc cgatgcgccg cggcgcgcag gcggatgcgg gcgtggagga cttcgagcgg ctgttcagcg cgcaggggct gatggcgacg tacttccgcg atcacctcgc cgcgtatgtg gacacgtcgg ccaagccgtg gcaggcgctg cgctcgaacg gcgggccgaa cgggatggtg agccagtcgg tgctgaactc gtacgagacg gccgagcgca tccgcggcgc gatgctcgac gattcggggc acctgcgcgt gtcgaccgtg ctgcgtttca tcgacatgga ttcgcagttg tccgaagcgc agctgagcgt ggctgggcag acggtgcgct tcgcgcacgg cgtgacctcg ccgcaccggg tggactggac caaccagaac acgcagctcg cgatcaagct gcagctcaaa tcggtggacg gccggatgac gacgctgcag ttcgacgggc cgtgggcgct gttccgcttc ttcgacgcgg ggcaggcggt gggcggcacg ggcaccgcgg accggcgcga gcggctgtat cagacgagcc tcggcacggt gcgcatcgaa tggcaggcgc tcacgctgcc gtcgccgata tggtcgggca tcctgcagtc gttcaggtgt ccgtcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for BumaA.19929.a.B2.PW38470

PURIFICATION DATe:	5/7/2018
CONCENTRATION:	10.41mg/ml
OBSERVED MW:	75kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	34
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGGCTCGA CGCTCATCGA GATCGCGAAC CAGATGCGCT ATGCGCAACT GCAGGCCGAG GACGCCGCGC AGGGCATGGC GACGCCGTAC TTCGAGCACG GGCTCGTCGC GGAGACCGCG CGCGAGACCT ACTACCGGCA CTTGCAGAAG ATGCTGATGC CGGAGTTGTA CAACGAGGTG CGGCGCACGC TCGTGTCGCA GGTCGACGGC AGCCCGGGCG ACATCTACCA GACGCTGAAG GTCTACCTGA TGCTCTGCCG CCCCGCGCGG CGCTCGGCGG ACGACGTCGT GCGCTGGCTC GACGGCCGCT GGGACGCGCT CTCGGGCGGG CAGTACTCGG ACGACGACCG CCGCTCGCTG CTCGCGCACG TGCGCACGCT GATGTCGCTG AAGGAGGTGC CGGCCACGCC GGAGGACGCG AACCTCGTGC GCTCGGCGCG CGCGAAGGCC GCGCAGATCC CGCTCGTCAC GCGCGTGCTG CAGCACATCC ACGCGCAGGG GCTGCCGCAG CAGGTCAACG ACATCTCGCT GTCGCGCGCG GCGGGCTTCG AGGCGTCGAT GAGCCTGCGC ATGCGCAGCA ACGTGCCGTC GACCGACACC GCGGTGTCGG GCTGGTTCAC GCGCGCGGGC TACACGGACG TGTTCCTGCC GCGCCTGCAG AAGAGCGCGC GCGCGATGCT CGAGGAGGAG AGCTGGGTGC TGCGCGACGA GACGCTGTCG GGCAACAGCT TCCAGATCGA CGGACTCGTG CAGAAGCTCG CCGATTCGGC GCGCAACCAG TTCCTGCAGG ACTACATCTC CGCGTGGCAG AACTTCCTGA ACGACGTGAC GGTGCGCGGC GTGACGGGCC TGGACGATGC GTCGCAGCTC GCCGCGGCGA TGATGGAGGC GCAATCGCCG CTCGCGAACC TGCTGCGCTT CGCCGCGCGC GAGACGACGC TCACGGGCGC GAGCGACGAA GGCAACATCG ACAGCTGGAT CGATCGCCAG AAGTACCGCT TCGAGAAGGG GCGGCGGCAG GTCGTCGGCG AGCTGAGCGG GCAGCATTAC CGCACGGTGC TGCTGCCGGA GCACGTGGTC GAGGAGCACT TCCAGGCGAT CCGCCAGCTC GCCGCGCAAC TGAACCGCAA CAACACGATC GCGAACAACC CGCTGTCGCG GCTGTTCGAG CCGCTGTACC GGCAGCTCGG GCTCGTCAAC GGCGCGCTGC AGGCGGGCCA GGTGCTGCCC GCGCAGTACG ACGCGTTCTC GCGGCTGAAG GAGACGGCCG CGCGCCAGCC GGAGCCGGTG CGCGGGATCA TGCTCGACCT CGTGAGCAGC GGCAGCACGA TGACGACGCG CGAATCGGGC GCGCTGCTCA ACCGGGGCGC GGCGGGCGCG ACGAAGATGG TCTGCGATCA GGGCTTCACG GGCCGCTATC CGATGCGCCG CGGCGCGCAG GCGGATGCGG GCGTGGAGGA CTTCGAGCGG CTGTTCAGCG CGCAGGGGCT GATGGCGACG TACTTCCGCG ATCACCTCGC CGCGTATGTG GACACGTCGG CCAAGCCGTG GCAGGCGCTG CGCTCGAACG GCGGGCCGAA CGGGATGGTG AGCCAGTCGG TGCTGAACTC GTACGAGACG GCCGAGCGCA TCCGCGGCGC GATGCTCGAC GATTCGGGGC ACCTGCGCGT GTCGACCGTG CTGCGTTTCA TCGACATGGA TTCGCAGTTG TCCGAAGCGC AGCTGAGCGT GGCTGGGCAG ACGGTGCGCT TCGCGCACGG CGTGACCTCG CCGCACCGGG TGGACTGGAC CAACCAGAAC ACGCAGCTCG CGATCAAGCT GCAGCTCAAA TCGGTGGACG GCCGGATGAC GACGCTGCAG TTCGACGGGC CGTGGGCGCT GTTCCGCTTC TTCGACGCGG GGCAGGCGGT GGGCGGCACG GGCACCGCGG ACCGGCGCGA GCGGCTGTAT CAGACGAGCC TCGGCACGGT GCGCATCGAA TGGCAGGCGC TCACGCTGCC GTCGCCGATA TGGTCGGGCA TCCTGCAGTC GTTCAGGTGT CCGTCGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T