BumaA.19928.a

Putative diaminobutyrate--pyruvate aminotransferase/membrane protein (BMAA1647)

CENTER ID: BumaA.19928.a
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.19928.a.B2.GE42218 transferase domain 1 432
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.5237
UniProt: A0A0H2WB96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHRFETIRQL ESSARTYASS FEAVFESGAG ALLRDQSGRE IIDCLSCAGA LPLGHNHPEV NDAIRRFFQS GHVQQALDLS TPAKFEFVKT LFETLPDDWA RRAKIQFCSP SGSDAVEAAM KLTRFATGRQ TIVAFSGAYH GMTRGALAAM GNLGVKSGLG LGAGDVHFAP YPYRFRCPFG TDGTETDALS IQYLRNLLAD PESGVSKPAA VVVEVVQGEG GCIPVSDAWL RALRQLTRAH EIALIIDEVQ TGFGRTGSMF AFERAGIRPD VLVLSKAIGG GFPLAVVVYD EVLDVWPRGK HAGTFRGNQI AMVAGKATIE ILRRDRLDAH AAQMGALLVD GLREIARAHP EFGDVRGRGL MVGVEIVDPS SDGAPQDGEL ARAIKLEAFR NGLLVETGGR HGAVLRLLPP LIVNRADISA ILERLDASIA QAKRGRGPRG GANHRSDERD GEAARPVAQG QSALPQAEPV KREAERRRGL LKPAFLLLWL GETALDFGSA LMSFALAAWI FQKTGSAERF SFAVLSAAIP ALLLTPVAGA LADRFDRRWV IAGCDVAMAI MIGALAWLLF RGALAPGHLY FFNATSAAIG CIRMPAYRAA VTAIVPRERL TQASGFTGTS QALLRIAAPL IAGYVLADYG LKGIVGLDML MIAAGSAAIF AALLRAAHAI RGVVGAVDAS LIEGVSASFA AAVRYFKSVP MFQGLAAYNM LQESLLLLSS VMLTPLVLST HSSSTLGLIL TYGALGGLAG SLLLLVLRIE ARLMSLALFA DAVLAMLIAL VGFSRSPAAW CAFVFLAYFA SSASSACTSA LWARKTPREM RGSIFALNTS MNLAAMSIVV LVGGVLCQRL FEPALADGGA WAPTVGAWLG TGKGRGIGLL LVLCGAAGCV ASLLALTCTR LRHLDALVPD QTHDDARDLL RNGVAVT
NT Sequence
atgcatcgct tcgaaacgat caggcagctt gaatccagtg cacggacgta tgcttcgagc ttcgaggccg ttttcgagtc cggcgcgggt gcgctgctgc gcgatcaatc aggccgggaa atcatcgatt gtctgtcgtg cgcgggggca ttgccgctcg ggcacaacca tcccgaagtc aacgatgcga tccggcgctt cttccagtcc ggccacgtgc agcaggcgct cgatctgtcc acgccggcca aattcgaatt cgtgaagacg ttgttcgaaa cacttccgga cgattgggcg cggcgcgcga agattcagtt ctgcagtccg agcggatcgg acgcggtcga ggccgcgatg aagttgacgc gcttcgcgac gggccgacag acgatcgtcg cgttctccgg cgcttatcac ggtatgacgc gcggcgcgct tgccgcgatg ggcaacctgg gggtgaagtc ggggcttggg ctgggcgcgg gcgatgtgca cttcgcgcct tacccgtatc gcttccgctg tccgttcggc acggacggca cggagacgga cgcgttatcg attcaatact tgcgcaatct gctcgcggac cccgaaagcg gcgtttcgaa gccggccgcc gtggtcgtcg aggtcgtgca gggcgagggc ggctgcattc cggtatcgga cgcttggctt cgcgcgctgc gccagttgac gcgggcgcac gagattgcgc tcatcatcga cgaagttcaa acgggtttcg gccgtaccgg ctcgatgttc gcattcgagc gcgcgggcat tcgtccggac gtcctggtgt tgtcgaaggc gatcggcggc ggttttccgc tggccgtcgt ggtgtacgac gaggtgctcg acgtctggcc gcgcggcaag cacgccggca cgtttcgcgg caaccagatc gcgatggtgg cgggcaaggc gacgatcgag atcctgcggc gcgatcgcct cgacgcacat gcggcgcaga tgggggcgct gctcgtcgat ggcttgcggg agatcgcacg tgcgcatccg gagttcggcg acgtcagggg gcgcgggttg atggtcggcg tcgagatcgt cgatccgtcg agcgacggcg cgccgcagga cggcgaactc gctcgcgcga tcaagctcga ggcgttcagg aacggcttgc tggttgaaac gggcgggcgc cacggtgcgg tgctgcgttt gctgccgccg ctcatcgtga atcgcgccga catctcggcc attctcgagc ggctcgatgc gtcgatcgcg caagccaagc gcgggcgcgg gccgcggggc ggcgcgaacc atcgctcgga cgagcgcgac ggcgaagcgg cgcggccggt tgcgcaaggc caatcggcac tgccgcaagc ggagccggtc aagcgggaag ccgagcgccg tcgtggcctg ctgaagccgg cgttcctgct gctctggctc ggcgagacgg cgctcgattt cggctccgcg ctgatgagct ttgcgctggc tgcatggatt ttccagaaga ccggctcggc cgagcgcttc tcgttcgcgg tgctttccgc ggcgattccc gcactgctgt tgacgcccgt ggcgggcgcg ctcgccgatc gcttcgatcg acgctgggtg atcgccggtt gcgatgtcgc gatggcgatc atgatcggcg cgctcgcgtg gttgctgttc aggggcgcgc tagcccccgg acatctctat tttttcaatg cgacgagcgc ggcgatcggc tgcatccgga tgcccgcgta tcgcgcggcg gtgaccgcga tcgtgccgag ggaacgcctc acgcaggcga gcggcttcac cggcacgagc caggcgctgt tgcggatcgc cgcgccgctg atcgccggct acgtgctcgc cgactacggg ctgaaaggca tcgtcggcct cgacatgctg atgatcgcgg ccggctcggc ggccatcttc gcggcgctac tgcgtgccgc gcacgcgatt cgcggcgtgg tcggcgcggt cgacgcttcg ctcattgagg gcgtgtcggc gagcttcgcg gccgccgtcc ggtacttcaa gtccgtgccg atgttccagg ggctcgccgc ctacaacatg ctgcaggaaa gccttctgct gctgagctcg gtgatgctca cgccgctcgt gctgtcgacc cattccagca gcacgctcgg cctgatcctc acatacggcg cgctcggtgg gctggcgggt tccttgctgc tgctggtgtt gcgcatcgaa gcaaggctga tgtcgctcgc gctgttcgcc gatgcggtgt tggcgatgct catcgcgctc gtcggcttca gccgctcgcc ggctgcgtgg tgcgcattcg tgttcctcgc gtatttcgcg agcagcgcgt cgagcgcatg cacgagtgcg ctgtgggcgc gcaagacgcc acgggaaatg cgcggcagca ttttcgcgtt gaacacgtcg atgaatctgg ccgcgatgtc gatcgtggtg ctggtgggcg gcgtgctgtg tcaacggctt ttcgagccgg cgctcgcgga cggcggtgca tgggcgccga ccgtcggtgc gtggctcggc accggcaagg ggcgcggcat cgggctgttg ctggtgctgt gcggcgccgc cggatgcgtt gcgtcgctgc tcgcgttgac ctgcacgcgc ttgcgtcatc tcgatgcgct ggtccccgat cagacgcacg acgatgctcg cgatttgctg cgcaacggcg tcgccgtgac gtga
Details for BumaA.19928.a.B2.GE42218
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMH RFETIRQLES SARTYASSFE AVFESGAGAL LRDQSGREII DCLSCAGALP LGHNHPEVND AIRRFFQSGH VQQALDLSTP AKFEFVKTLF ETLPDDWARR AKIQFCSPSG SDAVEAAMKL TRFATGRQTI VAFSGAYHGM TRGALAAMGN LGVKSGLGLG AGDVHFAPYP YRFRCPFGTD GTETDALSIQ YLRNLLADPE SGVSKPAAVV VEVVQGEGGC IPVSDAWLRA LRQLTRAHEI ALIIDEVQTG FGRTGSMFAF ERAGIRPDVL VLSKAIGGGF PLAVVVYDEV LDVWPRGKHA GTFRGNQIAM VAGKATIEIL RRDRLDAHAA QMGALLVDGL REIARAHPEF GDVRGRGLMV GVEIVDPSSD GAPQDGELAR AIKLEAFRNG LLVETGGRHG AVLRLLPPLI VNRADISAIL ER
Validated NT Sequence
gccgctcgag aatggccgag atgtcggcgc gattcacgat gagcggcggc agcaaacgca gcaccgcacc gtggcgcccg cccgtttcaa ccagcaagcc gttcctgaac gcctcgagct tgatcgcgcg agcgagttcg ccgtcctgcg gcgcgccgtc gctcgacgga tcgacgatct cgacgccgac catcaacccg cgccccctga cgtcgccgaa ctccggatgc gcacgtgcga tctcccgcaa gccatcgacg agcagcgccc ccatctgcgc cgcatgtgcg tcgaggcgat cgcgccgcag gatctcgatc gtcgccttgc ccgccaccat cgcgatctgg ttgccgcgaa acgtgccggc gtgcttgccg cgcggccaga cgtcgagcac ctcgtcgtac accacgacgg ccagcggaaa accgccgccg atcgccttcg acaacaccag gacgtccgga cgaatgcccg cgcgctcgaa tgcgaacatc gagccggtac ggccgaaacc cgtttgaact tcgtcgatga tgagcgcaat ctcgtgcgcc cgcgtcaact ggcgcagcgc gcgaagccaa gcgtccgata ccggaatgca gccgccctcg ccctgcacga cctcgacgac cacggcggcc ggcttcgaaa cgccgctttc ggggtccgcg agcagattgc gcaagtattg aatcgataac gcgtccgtct ccgtgccgtc cgtgccgaac ggacagcgga agcgatacgg gtaaggcgcg aagtgcacat cgcccgcgcc cagcccaagc cccgacttca cccccaggtt gcccatcgcg gcaagcgcgc cgcgcgtcat accgtgataa gcgccggaga acgcgacgat cgtctgtcgg cccgtcgcga agcgcgtcaa cttcatcgcg gcctcgaccg cgtccgatcc gctcggactg cagaactgaa tcttcgcgcg ccgcgcccaa tcgtccggaa gtgtttcgaa caacgtcttc acgaattcga atttggccgg cgtggacaga tcgagcgcct gctgcacgtg gccggactgg aagaagcgcc ggatcgcatc gttgacttcg ggatggttgt gcccgagcgg caatgccccc gcgcacgaca gacaatcgat gatttcccgg cctgattgat cgcgcagcag cgcacccgcg ccggactcga aaacggcctc gaagctcgaa gcatacgtcc gtgcactgga ttcaagctgc ctgatcgttt cgaagcgatg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMH RFETIRQLES SARTYASSFE AVFESGAGAL LRDQSGREII DCLSCAGALP LGHNHPEVND AIRRFFQSGH VQQALDLSTP AKFEFVKTLF ETLPDDWARR AKIQFCSPSG SDAVEAAMKL TRFATGRQTI VAFSGAYHGM TRGALAAMGN LGVKSGLGLG AGDVHFAPYP YRFRCPFGTD GTETDALSIQ YLRNLLADPE SGVSKPAAVV VEVVQGEGGC IPVSDAWLRA LRQLTRAHEI ALIIDEVQTG FGRTGSMFAF ERAGIRPDVL VLSKAIGGGF PLAVVVYDEV LDVWPRGKHA GTFRGNQIAM VAGKATIEIL RRDRLDAHAA QMGALLVDGL REIARAHPEF GDVRGRGLMV GVEIVDPSSD GAPQDGELAR AIKLEAFRNG LLVETGGRHG AVLRLLPPLI VNRADISAIL ERLDASIAQA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcatc gcttcgaaac gatcaggcag cttgaatcca gtgcacggac gtatgcttcg agcttcgagg ccgttttcga gtccggcgcg ggtgcgctgc tgcgcgatca atcaggccgg gaaatcatcg attgtctgtc gtgcgcgggg gcattgccgc tcgggcacaa ccatcccgaa gtcaacgatg cgatccggcg cttcttccag tccggccacg tgcagcaggc gctcgatctg tccacgccgg ccaaattcga attcgtgaag acgttgttcg aaacacttcc ggacgattgg gcgcggcgcg cgaagattca gttctgcagt ccgagcggat cggacgcggt cgaggccgcg atgaagttga cgcgcttcgc gacgggccga cagacgatcg tcgcgttctc cggcgcttat cacggtatga cgcgcggcgc gcttgccgcg atgggcaacc tgggggtgaa gtcggggctt gggctgggcg cgggcgatgt gcacttcgcg ccttacccgt atcgcttccg ctgtccgttc ggcacggacg gcacggagac ggacgcgtta tcgattcaat acttgcgcaa tctgctcgcg gaccccgaaa gcggcgtttc gaagccggcc gccgtggtcg tcgaggtcgt gcagggcgag ggcggctgca ttccggtatc ggacgcttgg cttcgcgcgc tgcgccagtt gacgcgggcg cacgagattg cgctcatcat cgacgaagtt caaacgggtt tcggccgtac cggctcgatg ttcgcattcg agcgcgcggg cattcgtccg gacgtcctgg tgttgtcgaa ggcgatcggc ggcggttttc cgctggccgt cgtggtgtac gacgaggtgc tcgacgtctg gccgcgcggc aagcacgccg gcacgtttcg cggcaaccag atcgcgatgg tggcgggcaa ggcgacgatc gagatcctgc ggcgcgatcg cctcgacgca catgcggcgc agatgggggc gctgctcgtc gatggcttgc gggagatcgc acgtgcgcat ccggagttcg gcgacgtcag ggggcgcggg ttgatggtcg gcgtcgagat cgtcgatccg tcgagcgacg gcgcgccgca ggacggcgaa ctcgctcgcg cgatcaagct cgaggcgttc aggaacggct tgctggttga aacgggcggg cgccacggtg cggtgctgcg tttgctgccg ccgctcatcg tgaatcgcgc cgacatctcg gccattctcg agcggctcga tgcgtcgatc gcgcaagcct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat