BumaA.19924.a

Orn/Lys/Arg decarboxylase (BMA0715)

CENTER ID: BumaA.19924.a
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.19924.a.B1.GE42217 full length 1 759
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.19924.a.B1.PW38464 full length 1 759

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.734
UniProt: A0A0H2WI47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFRFPVVII DEDFRSENIS GSGIRALAEA IEKEGVEVLG LTSYGDLTSF AQQSSRASCF ILSIDDDELM LGELGADGEL PELATAIIEL RAFVAEVRRR NADIPIFLYG ETRTSRHLPN DILRELHGFI HMFEDTPEFV ARHIIREAKV YLDSLAPPFF KELVKYADEG SYSWHCPGHS GGVAFLKNPL GQMFHQFFGE NMLRADVCNA VDELGQLLDH TGPVAASERN AARIFSADHL FFVTNGTSTS NKIVWHATVA PGDIVLVDRN CHKSILHAIT MTHAIPVFLT PTRNHFGIIG PIPRDEFKPE NIRKKIEANP FAREALRKNP NAKPRILTIT QSTYDGVVYN VEQIKDLLGD LVDTLHFDEA WLPHAEFHPF YQDMHAIGAG RPRTGALVFA THSTHKLLAG ISQASQIVVQ DSENRTFDKH RFNEAYLMHT STSPQYAIIA SCDVAAAMME PPGGTALVEE SIAEAIDFRR AMRKVDAEYG DDWFFSVWGP DNLSEEGIGS RADWLLKPND RWHGFGPLAP GFNMLDPIKA TIITPGLDVD GEFGETGIPA AIVTKYLAEH GIIVEKTGLY SFFIMFTIGI TKGRWNSMVT ELQQFKDDYD NNQPLWRVLP EFVAQYPLYE RVGLRDLCTQ IHDVYRANDI ARLTTEMYLS NMEPAMKPSD AFAKLAHREI DRVPLDELEG RVTSILLTPY PPGIPLLIPG ERFNKTIVNY LRFARDFNER FPGFHTDIHG LVAEEMNGRI EYYVDCVRD
NT Sequence
atgaagtttc gttttcccgt cgtcatcatc gacgaagatt tccgctccga gaacatctcg ggctccggca tccgggcgtt ggcggaggcg atcgagaaag aaggcgtcga ggtgctcggc ctgacgagct acggcgatct cacgtcgttc gcgcagcagt cgagccgcgc gtcgtgcttc atcctgtcga tcgacgacga cgaactgatg ctcggcgagc tcggcgcgga cggcgagctg cccgagctcg cgaccgcgat catcgagctg cgcgcgttcg tcgccgaagt gcgccgccgc aacgcggata tcccgatctt cctgtacggc gagacgcgca cgtcgcgcca tctgccgaac gacatcctgc gcgagctgca cggcttcatc cacatgttcg aggacacgcc ggagttcgtt gcgcgccaca tcatccgcga ggcgaaggtc tacctcgatt cgctcgcacc gccgttcttc aaggagctcg tcaagtacgc ggacgaaggc tcgtactcgt ggcactgccc ggggcactcc ggcggcgtcg cgttcctgaa gaacccgctc ggccagatgt tccaccagtt cttcggcgag aacatgctgc gcgcggacgt gtgcaacgcg gtcgacgagc ttggccagtt gctcgaccac acggggcccg tggccgcgtc cgagcgcaac gccgcgcgga tcttcagcgc cgatcacctg ttcttcgtga cgaacggcac gtcgacgtcg aacaagatcg tctggcacgc gacggtcgcg ccgggcgaca tcgtgctcgt cgatcgcaac tgccacaagt cgatcctgca cgcgatcacg atgacgcacg cgattccggt gttcctcacg ccgacgcgca accacttcgg catcatcggg ccgattccgc gcgacgagtt caagccggag aacatccgca agaagatcga ggcgaacccg ttcgcgcgcg aggcgctcag gaagaatccg aacgcgaagc cgcggatcct cacgatcacg cagagcacgt acgacggcgt cgtctacaac gtcgagcaga tcaaggatct gctcggcgat ctcgtcgaca cgctgcactt cgacgaagcg tggctgccgc acgccgagtt ccatccgttc taccaggaca tgcacgcgat cggcgcgggc cgcccgcgca cgggcgcgct cgtgttcgcg acgcactcga cgcacaagct gctcgcgggc atctcgcagg cctcgcagat cgtggtgcag gattcggaga accgcacgtt cgacaagcat cgcttcaacg aggcgtacct gatgcacacg tcgacgagcc cgcaatacgc gatcatcgcg tcgtgcgatg tcgcggccgc gatgatggag ccgccgggcg gcaccgcgct cgtcgaggaa tcgatcgccg aggcgatcga ctttcgccgc gcgatgcgca aggtggacgc cgagtacggc gacgactggt tcttcagcgt gtggggcccg gacaacctgt cggaggaggg aatcggctcg cgcgccgact ggctgctcaa gccgaacgac cgctggcacg gcttcggccc gctcgcgccg ggcttcaaca tgctcgaccc gatcaaggcg acgatcatca cgccggggct cgacgtcgac ggcgagttcg gcgaaacggg cattccggcc gcgatcgtca ccaagtatct ggccgagcac gggatcatcg tcgagaagac gggcctgtac tcgttcttca tcatgttcac gatcggcatc acgaagggcc gctggaactc gatggtcacc gagctgcagc agttcaagga cgactacgac aacaaccagc cgctctggcg cgtgctgccc gagttcgtcg cgcagtatcc gctctacgag cgcgtcggcc tgcgcgatct ctgcacgcag atccacgacg tgtaccgcgc gaacgacatc gcgcggctca cgaccgagat gtacctgtcg aacatggagc cggcgatgaa gccgtccgac gcgttcgcga agctcgcgca ccgcgagatc gaccgcgtgc cgctcgacga gctcgaagga cgcgtgacga gcatcctgct cacgccgtat ccgccgggca ttccgctgtt gatcccgggc gagcgcttca acaagacgat cgtcaactac ctgcggttcg cgcgcgattt caacgagcgc ttcccgggct ttcacacgga catccacggc ctcgtcgccg aagagatgaa cggccgcatc gagtactacg tcgactgcgt gcgcgattga
Details for BumaA.19924.a.B1.GE42217
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 86kDa
OBSERVED MW: 84kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK FRFPVVIIDE DFRSENISGS GIRALAEAIE KEGVEVLGLT SYGDLTSFAQ QSSRASCFIL SIDDDELMLG ELGADGELPE LATAIIELRA FVAEVRRRNA DIPIFLYGET RTSRHLPNDI LRELHGFIHM FEDTPEFVAR HIIREAKVYL DSLAPPFFKE LVKYADEGSY SWHCPGHSGG VAFLKNPLGQ MFHQFFGENM LRADVCNAVD ELGQLLDHTG PVAASERNAA RIFSADHLFF VTNGTSTSNK IVWHATVAPG DIVLVDRNCH KSILHAITMT HAIPVFLTPT RNHFGIIGPI PRDEFKPENI RKKIEANPFA REALRKNPNA KPRILTITQS TYDGVVYNVE QIKDLLGDLV DTLHFDEAWL PHAEFHPFYQ DMHAIGAGRP RTGALVFATH STHKLLAGIS QASQIVVQDS ENRTFDKHRF NEAYLMHTST SPQYAIIASC DVAAAMMEPP GGTALVEESI AEAIDFRRAM RKVDAEYGDD WFFSVWGPDN LSEEGIGSRA DWLLKPNDRW HGFGPLAPGF NMLDPIKATI ITPGLDVDGE FGETGIPAAI VTKYLAEHGI IVEKTGLYSF FIMFTIGITK GRWNSMVTEL QQFKDDYDNN QPLWRVLPEF VAQYPLYERV GLRDLCTQIH DVYRANDIAR LTTEMYLSNM EPAMKPSDAF AKLAHREIDR VPLDELEGRV TSILLTPYPP GIPLLIPGER FNKTIVNYLR FARDFNERFP GFHTDIHGLV AEEMNGRIEY YVDCVRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt ttcgttttcc cgtcgtcatc atcgacgaag atttccgctc cgagaacatc tcgggctccg gcatccgggc gttggcggag gcgatcgaga aagaaggcgt cgaggtgctc ggcctgacga gctacggcga tctcacgtcg ttcgcgcagc agtcgagccg cgcgtcgtgc ttcatcctgt cgatcgacga cgacgaactg atgctcggcg agctcggcgc ggacggcgag ctgcccgagc tcgcgaccgc gatcatcgag ctgcgcgcgt tcgtcgccga agtgcgccgc cgcaacgcgg atatcccgat cttcctgtac ggcgagacgc gcacgtcgcg ccatctgccg aacgacatcc tgcgcgagct gcacggcttc atccacatgt tcgaggacac gccggagttc gttgcgcgcc acatcatccg cgaggcgaag gtctacctcg attcgctcgc accgccgttc ttcaaggagc tcgtcaagta cgcggacgaa ggctcgtact cgtggcactg cccggggcac tccggcggcg tcgcgttcct gaagaacccg ctcggccaga tgttccacca gttcttcggc gagaacatgc tgcgcgcgga cgtgtgcaac gcggtcgacg agcttggcca gttgctcgac cacacggggc ccgtggccgc gtccgagcgc aacgccgcgc ggatcttcag cgccgatcac ctgttcttcg tgacgaacgg cacgtcgacg tcgaacaaga tcgtctggca cgcgacggtc gcgccgggcg acatcgtgct cgtcgatcgc aactgccaca agtcgatcct gcacgcgatc acgatgacgc acgcgattcc ggtgttcctc acgccgacgc gcaaccactt cggcatcatc gggccgattc cgcgcgacga gttcaagccg gagaacatcc gcaagaagat cgaggcgaac ccgttcgcgc gcgaggcgct caggaagaat ccgaacgcga agccgcggat cctcacgatc acgcagagca cgtacgacgg cgtcgtctac aacgtcgagc agatcaagga tctgctcggc gatctcgtcg acacgctgca cttcgacgaa gcgtggctgc cgcacgccga gttccatccg ttctaccagg acatgcacgc gatcggcgcg ggccgcccgc gcacgggcgc gctcgtgttc gcgacgcact cgacgcacaa gctgctcgcg ggcatctcgc aggcctcgca gatcgtggtg caggattcgg agaaccgcac gttcgacaag catcgcttca acgaggcgta cctgatgcac acgtcgacga gcccgcaata cgcgatcatc gcgtcgtgcg atgtcgcggc cgcgatgatg gagccgccgg gcggcaccgc gctcgtcgag gaatcgatcg ccgaggcgat cgactttcgc cgcgcgatgc gcaaggtgga cgccgagtac ggcgacgact ggttcttcag cgtgtggggc ccggacaacc tgtcggagga gggaatcggc tcgcgcgccg actggctgct caagccgaac gaccgctggc acggcttcgg cccgctcgcg ccgggcttca acatgctcga cccgatcaag gcgacgatca tcacgccggg gctcgacgtc gacggcgagt tcggcgaaac gggcattccg gccgcgatcg tcaccaagta tctggccgag cacgggatca tcgtcgagaa gacgggcctg tactcgttct tcatcatgtt cacgatcggc atcacgaagg gccgctggaa ctcgatggtc accgagctgc agcagttcaa ggacgactac gacaacaacc agccgctctg gcgcgtgctg cccgagttcg tcgcgcagta tccgctctac gagcgcgtcg gcctgcgcga tctctgcacg cagatccacg acgtgtaccg cgcgaacgac atcgcgcggc tcacgaccga gatgtacctg tcgaacatgg agccggcgat gaagccgtcc gacgcgttcg cgaagctcgc gcaccgcgag atcgaccgcg tgccgctcga cgagctcgaa ggacgcgtga cgagcatcct gctcacgccg tatccgccgg gcattccgct gttgatcccg ggcgagcgct tcaacaagac gatcgtcaac tacctgcggt tcgcgcgcga tttcaacgag cgcttcccgg gctttcacac ggacatccac ggcctcgtcg ccgaagagat gaacggccgc atcgagtact acgtcgactg cgtgcgcgat tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BumaA.19924.a.B1.PW38464
PURIFICATION DATe: 5/7/2018
CONCENTRATION: 16.53mg/ml
OBSERVED MW: 79kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM Lysine
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMK FRFPVVIIDE DFRSENISGS GIRALAEAIE KEGVEVLGLT SYGDLTSFAQ QSSRASCFIL SIDDDELMLG ELGADGELPE LATAIIELRA FVAEVRRRNA DIPIFLYGET RTSRHLPNDI LRELHGFIHM FEDTPEFVAR HIIREAKVYL DSLAPPFFKE LVKYADEGSY SWHCPGHSGG VAFLKNPLGQ MFHQFFGENM LRADVCNAVD ELGQLLDHTG PVAASERNAA RIFSADHLFF VTNGTSTSNK IVWHATVAPG DIVLVDRNCH KSILHAITMT HAIPVFLTPT RNHFGIIGPI PRDEFKPENI RKKIEANPFA REALRKNPNA KPRILTITQS TYDGVVYNVE QIKDLLGDLV DTLHFDEAWL PHAEFHPFYQ DMHAIGAGRP RTGALVFATH STHKLLAGIS QASQIVVQDS ENRTFDKHRF NEAYLMHTST SPQYAIIASC DVAAAMMEPP GGTALVEESI AEAIDFRRAM RKVDAEYGDD WFFSVWGPDN LSEEGIGSRA DWLLKPNDRW HGFGPLAPGF NMLDPIKATI ITPGLDVDGE FGETGIPAAI VTKYLAEHGI IVEKTGLYSF FIMFTIGITK GRWNSMVTEL QQFKDDYDNN QPLWRVLPEF VAQYPLYERV GLRDLCTQIH DVYRANDIAR LTTEMYLSNM EPAMKPSDAF AKLAHREIDR VPLDELEGRV TSILLTPYPP GIPLLIPGER FNKTIVNYLR FARDFNERFP GFHTDIHGLV AEEMNGRIEY YVDCVRD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGT TTCGTTTTCC CGTCGTCATC ATCGACGAAG ATTTCCGCTC CGAGAACATC TCGGGCTCCG GCATCCGGGC GTTGGCGGAG GCGATCGAGA AAGAAGGCGT CGAGGTGCTC GGCCTGACGA GCTACGGCGA TCTCACGTCG TTCGCGCAGC AGTCGAGCCG CGCGTCGTGC TTCATCCTGT CGATCGACGA CGACGAACTG ATGCTCGGCG AGCTCGGCGC GGACGGCGAG CTGCCCGAGC TCGCGACCGC GATCATCGAG CTGCGCGCGT TCGTCGCCGA AGTGCGCCGC CGCAACGCGG ATATCCCGAT CTTCCTGTAC GGCGAGACGC GCACGTCGCG CCATCTGCCG AACGACATCC TGCGCGAGCT GCACGGCTTC ATCCACATGT TCGAGGACAC GCCGGAGTTC GTTGCGCGCC ACATCATCCG CGAGGCGAAG GTCTACCTCG ATTCGCTCGC ACCGCCGTTC TTCAAGGAGC TCGTCAAGTA CGCGGACGAA GGCTCGTACT CGTGGCACTG CCCGGGGCAC TCCGGCGGCG TCGCGTTCCT GAAGAACCCG CTCGGCCAGA TGTTCCACCA GTTCTTCGGC GAGAACATGC TGCGCGCGGA CGTGTGCAAC GCGGTCGACG AGCTTGGCCA GTTGCTCGAC CACACGGGGC CCGTGGCCGC GTCCGAGCGC AACGCCGCGC GGATCTTCAG CGCCGATCAC CTGTTCTTCG TGACGAACGG CACGTCGACG TCGAACAAGA TCGTCTGGCA CGCGACGGTC GCGCCGGGCG ACATCGTGCT CGTCGATCGC AACTGCCACA AGTCGATCCT GCACGCGATC ACGATGACGC ACGCGATTCC GGTGTTCCTC ACGCCGACGC GCAACCACTT CGGCATCATC GGGCCGATTC CGCGCGACGA GTTCAAGCCG GAGAACATCC GCAAGAAGAT CGAGGCGAAC CCGTTCGCGC GCGAGGCGCT CAGGAAGAAT CCGAACGCGA AGCCGCGGAT CCTCACGATC ACGCAGAGCA CGTACGACGG CGTCGTCTAC AACGTCGAGC AGATCAAGGA TCTGCTCGGC GATCTCGTCG ACACGCTGCA CTTCGACGAA GCGTGGCTGC CGCACGCCGA GTTCCATCCG TTCTACCAGG ACATGCACGC GATCGGCGCG GGCCGCCCGC GCACGGGCGC GCTCGTGTTC GCGACGCACT CGACGCACAA GCTGCTCGCG GGCATCTCGC AGGCCTCGCA GATCGTGGTG CAGGATTCGG AGAACCGCAC GTTCGACAAG CATCGCTTCA ACGAGGCGTA CCTGATGCAC ACGTCGACGA GCCCGCAATA CGCGATCATC GCGTCGTGCG ATGTCGCGGC CGCGATGATG GAGCCGCCGG GCGGCACCGC GCTCGTCGAG GAATCGATCG CCGAGGCGAT CGACTTTCGC CGCGCGATGC GCAAGGTGGA CGCCGAGTAC GGCGACGACT GGTTCTTCAG CGTGTGGGGC CCGGACAACC TGTCGGAGGA GGGAATCGGC TCGCGCGCCG ACTGGCTGCT CAAGCCGAAC GACCGCTGGC ACGGCTTCGG CCCGCTCGCG CCGGGCTTCA ACATGCTCGA CCCGATCAAG GCGACGATCA TCACGCCGGG GCTCGACGTC GACGGCGAGT TCGGCGAAAC GGGCATTCCG GCCGCGATCG TCACCAAGTA TCTGGCCGAG CACGGGATCA TCGTCGAGAA GACGGGCCTG TACTCGTTCT TCATCATGTT CACGATCGGC ATCACGAAGG GCCGCTGGAA CTCGATGGTC ACCGAGCTGC AGCAGTTCAA GGACGACTAC GACAACAACC AGCCGCTCTG GCGCGTGCTG CCCGAGTTCG TCGCGCAGTA TCCGCTCTAC GAGCGCGTCG GCCTGCGCGA TCTCTGCACG CAGATCCACG ACGTGTACCG CGCGAACGAC ATCGCGCGGC TCACGACCGA GATGTACCTG TCGAACATGG AGCCGGCGAT GAAGCCGTCC GACGCGTTCG CGAAGCTCGC GCACCGCGAG ATCGACCGCG TGCCGCTCGA CGAGCTCGAA GGACGCGTGA CGAGCATCCT GCTCACGCCG TATCCGCCGG GCATTCCGCT GTTGATCCCG GGCGAGCGCT TCAACAAGAC GATCGTCAAC TACCTGCGGT TCGCGCGCGA TTTCAACGAG CGCTTCCCGG GCTTTCACAC GGACATCCAC GGCCTCGTCG CCGAAGAGAT GAACGGCCGC ATCGAGTACT ACGTCGACTG CGTGCGCGAT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT