SSGCID: Target Page

BumaA.19914.a

Acid phosphatase AcpA (BMA2888)

CENTER ID:	BumaA.19914.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.19914.a.B1.GE42213	full length		1	577

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.19914.a.B1.PW38463	full length		1	577

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.2959
UniProt:	A0A0H2WGS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRASPVRAPT RGGPSNQPED RGAEVTDKHP PSTDLPEDPD RRRVLGGIAA LGASVALAGC ETAVPAAPRS AAELRLDAAL RREVRNIVVI YAENRSFANL FGDFPGVQQP LGAVSPERYA QLDRDGKTPL ATLPKIWGGL VPQAQVVDGK RYMIGEHDIV GLPNAPFVIP DAQGKPLPNG VITRDLWHRF YQNQMQINGG RNDQFAAWAD SGGLVMGHYR NSAETLRLWN LARGYTLCDN FFMAAFGGSW LNHIFLISAQ APLYPDVHQS AAKHLVSVVE GDDPAGTRLK LAADSPASAL DGPPKFENDG LFTPDGYAVN TMAPPYQPSS VRPPVEGNPA YADPSNPRVL PPQHYATIGD RLSEKGVDWA WYGGAWQYAL EHRDTGSVPD FQYHHQPFNY FASYAPGTEA RRRHLRDAGL GDDPSTNHLI ADIDAGRLPT VTFYKPQGNL NMHAGYADVA SGDRHIATVI EHIMRGPQWA HTVVILTVDE NGGWWDPVSP PKGDRWGPGS RIPAMVISPF AKKGYVDHTL YDTNSILRFI SRVHGLAPLD GVLARERAFA ARGAQPPGDL TEALDLG

NT Sequence

atgcgcgcgt caccggttcg ggcgccgacg cgcggcggac cgtccaacca accagaagat cgaggagcag aagtgactga caagcatccc ccgtcgaccg accttcccga ggaccccgat cgccgccgcg tgctcggcgg catcgcggcg ctcggcgcga gcgtcgcgct cgccggctgc gagacggccg tgcccgccgc gccgcgctcg gccgcagaac tgcggctcga cgccgcgctg cgccgcgaag tgcgcaacat cgtcgtgatc tacgcggaga atcgcagctt cgcgaacctg ttcggcgatt tccccggcgt gcagcagccg ctcggcgcgg tatcgcccga gcgctacgcg cagctcgacc gcgacggcaa gacgccgctc gcgacgctgc cgaaaatctg gggcggcctc gtgccgcagg cgcaagtcgt cgacggcaag cgctacatga tcggcgagca cgacattgtc gggctgccga acgcgccgtt cgtgattccc gatgcgcagg gcaagccgct gccgaacggc gtgatcacgc gcgacttgtg gcatcgcttc tatcagaacc agatgcagat caacggcggc cgcaacgacc agttcgccgc ctgggccgat tcgggcggcc tcgtgatggg gcattaccgc aattcggccg agacgctgcg gctgtggaat ctcgcgcgcg ggtacacgct ctgcgacaac ttcttcatgg ccgcgttcgg cggctcgtgg ctgaaccaca tcttcctcat ttccgcgcag gcgccgcttt atccggacgt gcaccagagc gcggcgaagc atctcgtatc cgtcgtcgaa ggcgacgatc cggccggcac gcggctgaag ctggccgcgg attcgcccgc atcggcgctc gacggcccgc cgaaattcga gaacgatggc ctcttcacgc cggacggcta cgccgtgaac acgatggcgc cgccgtatca gccgagcagc gtgcgcccgc ccgtcgaagg caatccggcg tacgcggacc cgtcgaaccc gcgcgtgctg ccgccgcagc attacgcgac gatcggcgat cgcctgtcgg agaagggcgt cgactgggcg tggtacggcg gtgcgtggca atacgcgctc gagcatcggg acacgggctc ggtgccggac ttccagtatc accatcagcc gttcaattac ttcgcgagct acgcgccggg caccgaggcg cggcgccggc acctgcgcga cgcgggcctc ggcgacgatc cgtcgacgaa ccatctgatc gccgacatcg acgcgggccg cctgccgacc gtcacgttct acaagccgca gggcaacctg aacatgcacg cgggctatgc ggatgtcgcg tcgggggacc ggcacatcgc gacggtgatc gagcacatca tgcgcggccc gcagtgggcg cacacggtcg tgatcctgac ggtcgacgag aacggcggct ggtgggaccc ggtgtcgccg ccgaagggcg accggtgggg gccgggttcg cggattccgg cgatggtgat ctcgccgttc gcgaagaagg gctacgtcga tcacacgctg tacgacacga actcgatcct gcgtttcatc tcgcgcgtgc acgggctcgc gccgctcgac ggcgtgctcg cgcgtgagcg cgcgttcgcg gcgcgcggcg cgcagccgcc gggcgatctc accgaagcgc tcgacctcgg ctga

Details for BumaA.19914.a.B1.GE42213

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	64kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMR ASPVRAPTRG GPSNQPEDRG AEVTDKHPPS TDLPEDPDRR RVLGGIAALG ASVALAGCET AVPAAPRSAA ELRLDAALRR EVRNIVVIYA ENRSFANLFG DFPGVQQPLG AVSPERYAQL DRDGKTPLAT LPKIWGGLVP QAQVVDGKRY MIGEHDIVGL PNAPFVIPDA QGKPLPNGVI TRDLWHRFYQ NQMQINGGRN DQFAAWADSG GLVMGHYRNS AETLRLWNLA RGYTLCDNFF MAAFGGSWLN HIFLISAQAP LYPDVHQSAA KHLVSVVEGD DPAGTRLKLA ADSPASALDG PPKFENDGLF TPDGYAVNTM APPYQPSSVR PPVEGNPAYA DPSNPRVLPP QHYATIGDRL SEKGVDWAWY GGAWQYALEH RDTGSVPDFQ YHHQPFNYFA SYAPGTEARR RHLRDAGLGD DPSTNHLIAD IDAGRLPTVT FYKPQGNLNM HAGYADVASG DRHIATVIEH IMRGPQWAHT VVILTVDENG GWWDPVSPPK GDRWGPGSRI PAMVISPFAK KGYVDHTLYD TNSILRFISR VHGLAPLDGV LARERAFAAR GAQPPGDLTE ALDLG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg cgtcaccggt tcgggcgccg acgcgcggcg gaccgtccaa ccaaccagaa gatcgaggag cagaagtgac tgacaagcat cccccgtcga ccgaccttcc cgaggacccc gatcgccgcc gcgtgctcgg cggcatcgcg gcgctcggcg cgagcgtcgc gctcgccggc tgcgagacgg ccgtgcccgc cgcgccgcgc tcggccgcag aactgcggct cgacgccgcg ctgcgccgcg aagtgcgcaa catcgtcgtg atctacgcgg agaatcgcag cttcgcgaac ctgttcggcg atttccccgg cgtgcagcag ccgctcggcg cggtatcgcc cgagcgctac gcgcagctcg accgcgacgg caagacgccg ctcgcgacgc tgccgaaaat ctggggcggc ctcgtgccgc aggcgcaagt cgtcgacggc aagcgctaca tgatcggcga gcacgacatt gtcgggctgc cgaacgcgcc gttcgtgatt cccgatgcgc agggcaagcc gctgccgaac ggcgtgatca cgcgcgactt gtggcatcgc ttctatcaga accagatgca gatcaacggc ggccgcaacg accagttcgc cgcctgggcc gattcgggcg gcctcgtgat ggggcattac cgcaattcgg ccgagacgct gcggctgtgg aatctcgcgc gcgggtacac gctctgcgac aacttcttca tggccgcgtt cggcggctcg tggctgaacc acatcttcct catttccgcg caggcgccgc tttatccgga cgtgcaccag agcgcggcga agcatctcgt atccgtcgtc gaaggcgacg atccggccgg cacgcggctg aagctggccg cggattcgcc cgcatcggcg ctcgacggcc cgccgaaatt cgagaacgat ggcctcttca cgccggacgg ctacgccgtg aacacgatgg cgccgccgta tcagccgagc agcgtgcgcc cgcccgtcga aggcaatccg gcgtacgcgg acccgtcgaa cccgcgcgtg ctgccgccgc agcattacgc gacgatcggc gatcgcctgt cggagaaggg cgtcgactgg gcgtggtacg gcggtgcgtg gcaatacgcg ctcgagcatc gggacacggg ctcggtgccg gacttccagt atcaccatca gccgttcaat tacttcgcga gctacgcgcc gggcaccgag gcgcggcgcc ggcacctgcg cgacgcgggc ctcggcgacg atccgtcgac gaaccatctg atcgccgaca tcgacgcggg ccgcctgccg accgtcacgt tctacaagcc gcagggcaac ctgaacatgc acgcgggcta tgcggatgtc gcgtcggggg accggcacat cgcgacggtg atcgagcaca tcatgcgcgg cccgcagtgg gcgcacacgg tcgtgatcct gacggtcgac gagaacggcg gctggtggga cccggtgtcg ccgccgaagg gcgaccggtg ggggccgggt tcgcggattc cggcgatggt gatctcgccg ttcgcgaaga agggctacgt cgatcacacg ctgtacgaca cgaactcgat cctgcgtttc atctcgcgcg tgcacgggct cgcgccgctc gacggcgtgc tcgcgcgtga gcgcgcgttc gcggcgcgcg gcgcgcagcc gccgggcgat ctcaccgaag cgctcgacct cggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BumaA.19914.a.B1.PW38463

PURIFICATION DATe:	5/7/2018
CONCENTRATION:	16.91mg/ml
OBSERVED MW:	64kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 1mM ATP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCG CGTCACCGGT TCGGGCGCCG ACGCGCGGCG GACCGTCCAA CCAACCAGAA GATCGAGGAG CAGAAGTGAC TGACAAGCAT CCCCCGTCGA CCGACCTTCC CGAGGACCCC GATCGCCGCC GCGTGCTCGG CGGCATCGCG GCGCTCGGCG CGAGCGTCGC GCTCGCCGGC TGCGAGACGG CCGTGCCCGC CGCGCCGCGC TCGGCCGCAG AACTGCGGCT CGACGCCGCG CTGCGCCGCG AAGTGCGCAA CATCGTCGTG ATCTACGCGG AGAATCGCAG CTTCGCGAAC CTGTTCGGCG ATTTCCCCGG CGTGCAGCAG CCGCTCGGCG CGGTATCGCC CGAGCGCTAC GCGCAGCTCG ACCGCGACGG CAAGACGCCG CTCGCGACGC TGCCGAAAAT CTGGGGCGGC CTCGTGCCGC AGGCGCAAGT CGTCGACGGC AAGCGCTACA TGATCGGCGA GCACGACATT GTCGGGCTGC CGAACGCGCC GTTCGTGATT CCCGATGCGC AGGGCAAGCC GCTGCCGAAC GGCGTGATCA CGCGCGACTT GTGGCATCGC TTCTATCAGA ACCAGATGCA GATCAACGGC GGCCGCAACG ACCAGTTCGC CGCCTGGGCC GATTCGGGCG GCCTCGTGAT GGGGCATTAC CGCAATTCGG CCGAGACGCT GCGGCTGTGG AATCTCGCGC GCGGGTACAC GCTCTGCGAC AACTTCTTCA TGGCCGCGTT CGGCGGCTCG TGGCTGAACC ACATCTTCCT CATTTCCGCG CAGGCGCCGC TTTATCCGGA CGTGCACCAG AGCGCGGCGA AGCATCTCGT ATCCGTCGTC GAAGGCGACG ATCCGGCCGG CACGCGGCTG AAGCTGGCCG CGGATTCGCC CGCATCGGCG CTCGACGGCC CGCCGAAATT CGAGAACGAT GGCCTCTTCA CGCCGGACGG CTACGCCGTG AACACGATGG CGCCGCCGTA TCAGCCGAGC AGCGTGCGCC CGCCCGTCGA AGGCAATCCG GCGTACGCGG ACCCGTCGAA CCCGCGCGTG CTGCCGCCGC AGCATTACGC GACGATCGGC GATCGCCTGT CGGAGAAGGG CGTCGACTGG GCGTGGTACG GCGGTGCGTG GCAATACGCG CTCGAGCATC GGGACACGGG CTCGGTGCCG GACTTCCAGT ATCACCATCA GCCGTTCAAT TACTTCGCGA GCTACGCGCC GGGCACCGAG GCGCGGCGCC GGCACCTGCG CGACGCGGGC CTCGGCGACG ATCCGTCGAC GAACCATCTG ATCGCCGACA TCGACGCGGG CCGCCTGCCG ACCGTCACGT TCTACAAGCC GCAGGGCAAC CTGAACATGC ACGCGGGCTA TGCGGATGTC GCGTCGGGGG ACCGGCACAT CGCGACGGTG ATCGAGCACA TCATGCGCGG CCCGCAGTGG GCGCACACGG TCGTGATCCT GACGGTCGAC GAGAACGGCG GCTGGTGGGA CCCGGTGTCG CCGCCGAAGG GCGACCGGTG GGGGCCGGGT TCGCGGATTC CGGCGATGGT GATCTCGCCG TTCGCGAAGA AGGGCTACGT CGATCACACG CTGTACGACA CGAACTCGAT CCTGCGTTTC ATCTCGCGCG TGCACGGGCT CGCGCCGCTC GACGGCGTGC TCGCGCGTGA GCGCGCGTTC GCGGCGCGCG GCGCGCAGCC GCCGGGCGAT CTCACCGAAG CGCTCGACCT CGGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT