SSGCID: Target Page

BumaA.19910.a

Uncharacterized protein (BMA1011)

CENTER ID:	BumaA.19910.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.19910.a.B2.GE42209	mature protein		30	253

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.1051
UniProt:	A0A0H2WIG2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKSSKISKTL ICSLLAFSAT AFSIHPVLAA SPQGGKIATT IQAAQGQTDI AALFKKTNFQ MDSAAEYGTV MQAIAANESA AVTYVQDSRL SATEVQTTHP IWSGVQNGDR LGVNWSDGDF QHQRIFVYEG NGNAGKWKKA EAADIPLKQG GAPSILNQTP LMPQQQVRET SASAKSVADV VSVSLYRRVT GNIDIYTVKV GSRIQGIYGV GMTAPGWIQA DGVGTDIVVF QVRASGTPGL FYSGVASATV IRE

NT Sequence

atgaagtcaa gcaagatatc caaaacactg atttgctcgc ttctcgcatt ttccgctacc gctttttcga ttcatcctgt tctcgcggcc tccccacagg gcggcaagat cgcgacaacg atccaggcgg ctcaaggcca aacggatatc gccgctttat tcaagaaaac gaatttccaa atggattcgg cggccgaata cggaacggtg atgcaggcca tcgccgcgaa cgaaagcgcc gctgtcacgt atgtgcaaga cagccgcctg tcggccaccg aggttcaaac gacgcatccg atctggagcg gcgtacagaa tggcgacagg ctcggcgtca actggagcga cggtgatttt cagcatcaac ggatcttcgt ctacgaaggc aacggcaatg ccgggaagtg gaagaaagcc gaggcggcgg acattccgct caagcagggc ggtgcgcctt ccatcctgaa tcagacgccg ctgatgccgc agcagcaggt gcgcgaaacg tccgcgtcgg ccaagagtgt cgcggacgtc gtttccgtgt cgctatatcg acgagtgact ggaaatatcg atatctatac ggtaaaagtg ggcagcagga ttcaaggcat ttacggtgtc ggcatgactg ctcccggctg gattcaagcc gacggcgtcg gaacggatat cgtcgtattt caggtccgcg cctccgggac acccggtttg ttttatagcg gcgtggcgag cgccaccgtc atccgcgagt ga

Details for BumaA.19910.a.B2.GE42209

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHYMA SPQGGKIATT IQAAQGQTDI AALFKKTNFQ MDSAAEYGTV MQAIAANESA AVTYVQDSRL SATEVQTTHP IWSGVQNGDR LGVNWSDGDF QHQRIFVYEG NGNAGKWKKA EAADIPLKQG GAPSILNQTP LMPQQQVRET SASAKSVADV VSVSLYRRVT GNIDIYTVKV GSRIQGIYGV GMTAPGWIQA DGVGTDIVVF QVRASGTPGL FYSGVASATV IRE

Validated NT Sequence

atcttactca cttactcgcg gatgacggtg gcgctcgcca cgccgctata aaacaaaccg ggtgtcccgg aggcgcggac ctgaaatacg acgatatccg ttccgacgcc gtcggcttga atccagccgg gagcagtcat gccgacaccg taaatgcctt gaatcctgct gcccactttt accgtataga tatcgatatt tccagtcact cgtcgatata gcgacacgga aacgacgtcc gcgacactct tggccgacgc ggacgtttcg cgcacctgct gctgcggcat cagcggcgtc tgattcagga tggaaggcgc accgccctgc ttgagcggaa tgtccgccgc ctcggctttc ttccacttcc cggcattgcc gttgccttcg tagacgaaga tccgttgatg ctgaaaatca ccgtcgctcc agttgacgcc gagcctgtcg ccattctgta cgccgctcca gatcggatgc gtcgtttgaa cctcggtggc cgacaggcgg ctgtcttgca catacgtgac agcggcgctt tcgttcgcgg cgatggcctg catcaccgtt ccgtattcgg ccgccgaatc catttggaaa ttcgttttct tgaataaagc ggcgatatcc gtttggcctt gagccgcctg gatcgttgtc gcgatcttgc cgccctgtgg ggaggccata taatggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHAS PQGGKIATTI QAAQGQTDIA ALFKKTNFQM DSAAEYGTVM QAIAANESAA VTYVQDSRLS ATEVQTTHPI WSGVQNGDRL GVNWSDGDFQ HQRIFVYEGN GNAGKWKKAE AADIPLKQGG APSILNQTPL MPQQQVRETS ASAKSVADVV SVSLYRRVTG NIDIYTVKVG SRIQGIYGVG MTAPGWIQAD GVGTDIVVFQ VRASGTPGLF YSGVASATVI RE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcctccc cacagggcgg caagatcgcg acaacgatcc aggcggctca aggccaaacg gatatcgccg ctttattcaa gaaaacgaat ttccaaatgg attcggcggc cgaatacgga acggtgatgc aggccatcgc cgcgaacgaa agcgccgctg tcacgtatgt gcaagacagc cgcctgtcgg ccaccgaggt tcaaacgacg catccgatct ggagcggcgt acagaatggc gacaggctcg gcgtcaactg gagcgacggt gattttcagc atcaacggat cttcgtctac gaaggcaacg gcaatgccgg gaagtggaag aaagccgagg cggcggacat tccgctcaag cagggcggtg cgccttccat cctgaatcag acgccgctga tgccgcagca gcaggtgcgc gaaacgtccg cgtcggccaa gagtgtcgcg gacgtcgttt ccgtgtcgct atatcgacga gtgactggaa atatcgatat ctatacggta aaagtgggca gcaggattca aggcatttac ggtgtcggca tgactgctcc cggctggatt caagccgacg gcgtcggaac ggatatcgtc gtatttcagg tccgcgcctc cgggacaccc ggtttgtttt atagcggcgt ggcgagcgcc accgtcatcc gcgagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat