BumaA.19909.a

Mechanosensitive ion channel family (BMA0986)

CENTER ID: BumaA.19909.a
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.19909.a.B2.GE42208 MscS-like soluble domain 655 801
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.1024
UniProt: A0A0H2WIG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRLATFARR VALIGLLHLL CAALPAAAAD LASGASVPPA PAISRDEALA ELKRVQAALD RIKQQASAAT TYKQLDALDE STQALSADVD KLTAALVPTR AQLQAQLDVL GPPPAPGAAP ETPAVARQRA DLNARKTQLD AALKQAADEK ESLANLTQQY SKLRRSLLRD QLVLRSSSIL GPRFWAPLFR PSDDDRQELG AFSAQIGQVL AAAWEPGRRL GTALLLAAAL AVWTLGRRLA ERALAWFSLT KLPETRVRRS ALATSIALSS VVTTGIAVQF VYLALTRDYA LSSALQDFAD ELAKLALTCA LIAGLGRALL CTHHPSWRLP ALADEVALAM RPFPAILAAL LLVAGALEEL NRTADTSLQV TLFGRGIVSL VVVTIGASLL RANRARTALA AAGEPPEARS TLAGLIHAGV TLTVVAALAA LLIGYISVAR FLTYELVWFE IVIGSVYLLT RLTRDAYASA FAANLSSGRL IKHLFGLNDS HLDQARTVLT GISTSVLLLV AVIALLTGGF GTTPNDLLVS VISMFGGDKL QRLNIVPARI LNAALALAIG IYLLRSVRRW LDNELLPAIG LDQGMRASLV TLFSNLGYVA LVLLALSLLG VKWDNLAWIV SALSVGIGFG LQEIVKNFVS GLILLTERPV KVGDMISISG VEGDIRRINV RATEIQLSDR STVIVPNSQL ISQNLRNVTM GNSTQGVATL MLTFPLNTDP EQVRDLLLDA YREHASVLEK PAPSVTFSQL TPDGITLSVT GYVGSPRIAA ATKSDLLFEI LKRLRAAGIA LSNPQTLMVQ NLPPPSSE
NT Sequence
atgatccgtc ttgcgacatt cgcgcgccgc gtcgcgctga taggcctatt gcatctgctc tgcgcggcgt tgcccgcggc ggcggccgac ctcgcgtccg gcgcgagcgt gccgcccgcg ccggcgatct cgcgtgacga ggcgctcgcc gaactgaagc gcgtgcaggc cgcgctcgac cggatcaagc agcaggcgtc ggccgcgacc acctacaagc aactggacgc gctcgacgaa tcgacgcaag cgctgagcgc cgacgtcgac aagctgacgg cggcgctcgt gcccacgcgc gcgcagcttc aggcgcagct cgacgtgctc gggccgccgc ccgcgccggg cgcggcgccc gaaacgcccg ccgtcgcgcg gcagcgcgcg gacctgaacg cgcgcaagac gcagctcgac gccgcgctca agcaggccgc cgacgagaag gaaagcctcg cgaacctgac acagcaatac tcgaagctgc gccgcagcct gctgcgcgac cagctcgtgc tgcgctcgag cagcattctc ggcccgcggt tctgggcccc gctctttcgt ccgtcggacg atgatcggca ggaactcggc gcgttcagcg cgcagatcgg gcaagtgctt gccgcggcat gggagccggg ccgccggctc ggcaccgcgc tgctgctcgc cgccgcgctc gccgtgtgga cgctcggcag gcggctcgcc gagcgcgcgc tcgcgtggtt cagcctgacg aagctgccgg agacgcgcgt gcggcgcagc gcgctcgcga cgtcgatcgc gctgtcgagc gtcgtgacga cgggcatcgc cgtgcaattc gtctatctcg cgctcacgcg cgactacgcg ctgtcgagcg cgctgcagga tttcgccgac gagctcgcga agctcgcgct gacttgcgcg ctgatcgcgg ggctcgggcg cgcgctgctg tgcacccacc atccgagctg gcggctgccc gcgcttgccg acgaagtcgc gctcgcgatg cggccgttcc cggcgatcct cgcggcgctg ctgctcgtcg cgggcgcgct cgaggaactg aaccgaaccg ccgacacgag cctgcaggtc acgctgttcg ggcgcggaat cgtgtcgctc gtcgtcgtga cgatcggcgc gtcgctgctg cgcgcgaacc gcgcgcgcac cgcgctcgcc gccgcgggcg agccgcccga ggcgcgctcg acgctcgcgg ggctgatcca cgcgggcgtc acgctgaccg tcgtcgcggc gctcgccgcg ctcctcatcg gctacatctc ggtcgcgcgc tttctcacct atgagctcgt gtggttcgag atcgtgatcg gcagcgtgta cttgctgacg cgcctcaccc gcgacgcata cgcgagcgcg ttcgccgcga acctgtcgag cggccggctg atcaagcatc tgttcgggct gaacgacagc cacctcgatc aggcacgcac cgtgctgacc ggaatcagca cgagcgtgct gctgctcgtc gccgtcatcg cgctgctgac gggcggcttc ggcacgacgc cgaacgatct gctcgtgagc gtgatctcga tgttcggcgg cgacaaactg caacgcctga acatcgtgcc cgcccgcatc ctgaacgcgg cgctcgcgct ggcgatcggc atctatctgc tgcgctcggt gcgccgctgg ctggacaacg agctgctgcc ggcgatcggc ctggatcagg gcatgcgcgc gtcgctcgtc acgctgttca gcaatctcgg ctatgtcgcg ctcgtgctgc tcgcgctgtc gctgctcggc gtcaaatggg acaacctcgc gtggatcgtg agcgcgctgt cggtgggcat cggcttcggc ctgcaggaga tcgtgaagaa cttcgtgtcc gggctgatcc tgctcaccga gcggcccgtg aaggtcggcg acatgatcag catctcgggc gtcgaaggcg acatccgccg gatcaacgtg cgcgcgaccg agatccagct gtccgaccgc tcgacggtga tcgtgccgaa ctcgcaattg atctcgcaga acctgcgtaa cgtgacgatg ggcaacagca cgcagggcgt cgcgacgctg atgctcacgt ttccgctcaa caccgatccc gagcaggtgc gcgatctgct gctcgacgca taccgcgagc acgcgtcggt gctcgagaag cccgcgccgt ccgtcacctt cagccagctg acgccggacg ggatcacgct cagcgtgacg gggtacgtcg gcagcccgcg gatcgcggcg gcgacgaaga gcgacctgct gttcgagatt ctcaagcgac tgcgcgcggc gggcatcgcg ctgtcgaatc cgcagacgct gatggtgcag aatctgccgc cgccttcgag cgagtga
Details for BumaA.19909.a.B2.GE42208
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMI SISGVEGDIR RINVRATEIQ LSDRSTVIVP NSQLISQNLR NVTMGNSTQG VATLMLTFPL NTDPEQVRDL LLDAYREHAS VLEKPAPSVT FSQLTPDGIT LSVTGYVGSP RIAAATKSDL LFEILKRLRA AGIALSNPQT LMVQN
Validated NT Sequence
atcttactca cttaattctg caccatcagc gtctgcggat tcgacagcgc gatgcccgcc gcgcgcagtc gcttgagaat ctcgaacagc aggtcgctct tcgtcgccgc cgcgatccgc gggctgccga cgtaccccgt cacgctgagc gtgatcccgt ccggcgtcag ctggctgaag gtgacggacg gcgcgggctt ctcgagcacc gacgcgtgct cgcggtatgc gtcgagcagc agatcgcgca cctgctcggg atcggtgttg agcggaaacg tgagcatcag cgtcgcgacg ccctgcgtgc tgttgcccat cgtcacgtta cgcaggttct gcgagatcaa ttgcgagttc ggcacgatca ccgtcgagcg gtcggacagc tggatctcgg tcgcgcgcac gttgatccgg cggatgtcgc cttcgacgcc cgagatgctg atcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMI SISGVEGDIR RINVRATEIQ LSDRSTVIVP NSQLISQNLR NVTMGNSTQG VATLMLTFPL NTDPEQVRDL LLDAYREHAS VLEKPAPSVT FSQLTPDGIT LSVTGYVGSP RIAAATKSDL LFEILKRLRA AGIALSNPQT LMVQN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca gcatctcggg cgtcgaaggc gacatccgcc ggatcaacgt gcgcgcgacc gagatccagc tgtccgaccg ctcgacggtg atcgtgccga actcgcaatt gatctcgcag aacctgcgta acgtgacgat gggcaacagc acgcagggcg tcgcgacgct gatgctcacg tttccgctca acaccgatcc cgagcaggtg cgcgatctgc tgctcgacgc ataccgcgag cacgcgtcgg tgctcgagaa gcccgcgccg tccgtcacct tcagccagct gacgccggac gggatcacgc tcagcgtgac ggggtacgtc ggcagcccgc ggatcgcggc ggcgacgaag agcgacctgc tgttcgagat tctcaagcga ctgcgcgcgg cgggcatcgc gctgtcgaat ccgcagacgc tgatggtgca gaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat