SSGCID: Target Page

BumaA.18695.a

Autoinducer synthetase family protein

CENTER ID:	BumaA.18695.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.18695.a.B1.GE37741	Full length(BumaA.18695.a)		1	203

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.4908
RefSeq:	YP_105963.1
UniProt:	A0A0H2WCK4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRTFVHGDGR LPSDLAADLG LYRHGVFVEQ LGWKLPSASE GFERDQYDRD DTVYVFARDD DGEICGCARL LPTTRPYLLK ELFPTLVAQD MPLPQSAAVW ELSRFAANAE DPAGGGNPAW AVRPMLAAVV ECAARLGAKQ LIGVTFLSME RLFRRIGVHA HRAGPAQQID GRMVVACWID LDAQTLAALD LDPLLCAPPA EAA

NT Sequence

ATGCGAACTT TCGTTCATGG CGACGGGCGC CTGCCGAGCG ACTTGGCGGC TGATCTGGGC CTTTATCGGC ACGGAGTTTT CGTCGAGCAG CTCGGCTGGA AACTGCCGTC GGCAAGCGAA GGGTTCGAGC GGGATCAGTA CGATCGCGAC GATACCGTCT ATGTGTTCGC CCGCGACGAC GACGGGGAAA TCTGCGGCTG CGCCCGGCTG CTGCCGACGA CCCGCCCGTA TCTGCTGAAG GAACTGTTCC CGACGCTGGT CGCGCAAGAC ATGCCGTTGC CGCAATCCGC CGCCGTCTGG GAATTGTCGC GCTTCGCCGC GAACGCCGAG GATCCGGCCG GGGGCGGCAA CCCGGCCTGG GCGGTGCGGC CGATGCTCGC CGCCGTCGTC GAGTGCGCCG CGCGGCTTGG CGCGAAGCAA CTGATCGGCG TGACGTTTCT GAGCATGGAG CGCCTGTTCC GCCGGATCGG CGTGCACGCG CACCGGGCGG GGCCCGCGCA GCAGATCGAC GGGCGCATGG TCGTCGCGTG CTGGATCGAC CTCGACGCGC AAACGCTCGC CGCGCTCGAT CTCGACCCGC TGCTGTGCGC GCCGCCCGCC GAAGCCGCC

Details for BumaA.18695.a.B1.GE37741

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR TFVHGDGRLP SDLAADLGLY RHGVFVEQLG WKLPSASEGF ERDQYDRDDT VYVFARDDDG EICGCARLLP TTRPYLLKEL FPTLVAQDMP LPQSAAVWEL SRFAANAEDP AGGGNPAWAV RPMLAAVVEC AARLGAKQLI GVTFLSMERL FRRIGVHAHR AGPAQQIDGR MVVACWIDLD AQTLAALDLD PLLCAPPAEA A

Validated NT Sequence

gggggnnntn nncagccgga tcctatctta ctcacttagg cggcttcggc gggcggcgcg cacagcagcg ggtcgagatc gagcgcggcg agcgtttgcg cgtcgaggtc gatccagcac gcgacgacca tgcgcccgtc gatctgctgc gcgggccccg cccggtgcgc gtgcacgccg atccggcgga acaggcgctc catgctcaga aacgtcacgc cgatcagttg cttcgcgcca agccgcgcgg cgcactcgac gacggcggcg agcatcggcc gcaccgccca ggccgggttg ccgcccccgg ccggatcctc ggcgttcgcg gcgaagcgcg acaattccca gacggcggcg gattgcggca acggcatgtc ttgcgcgacc agcgtcggga acagttcctt cagcagatac gggcgggtcg tcggcagcag ccgggcgcag ccgcagattt ccccgtcgtc gtcgcgggcg aacacataga cggtatcgtc gcgatcgtac tgatcccgct cgaacccttc gcttgccgac ggcagtttcc agccgagctg ctcgacgaaa actccgtgcc gataaaggcc cagatcagcc gccaagtcgc tcggcaggcg cccgtcgcca tgaacgaaag ttcgcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgaa ctttcgttca tggcgacggg cgcctgccga gcgacttggc ggctgatctg ggcctttatc ggcacggagt tttcgtcgag cagctcggct ggaaactgcc gtcggcaagc gaagggttcg agcgggatca gtacgatcgc gacgataccg tctatgtgtt cgcccgcgac gacgacgggg aaatctgcgg ctgcgcccgg ctgctgccga cgacccgccc gtatctgctg aaggaactgt tcccgacgct ggtcgcgcaa gacatgccgt tgccgcaatc cgccgccgtc tgggaattgt cgcgcttcgc cgcgaacgcc gaggatccgg ccgggggcgg caacccggcc tgggcggtgc ggccgatgct cgccgccgtc gtcgagtgcg ccgcgcggct tggcgcgaag caactgatcg gcgtgacgtt tctgagcatg gagcgcctgt tccgccggat cggcgtgcac gcgcaccggg cggggcccgc gcagcagatc gacgggcgca tggtcgtcgc gtgctggatc gacctcgacg cgcaaacgct cgccgcgctc gatctcgacc cgctgctgtg cgcgccgccc gccgaagccg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat