SSGCID: Target Page

BumaA.18056.a

T6SS-2 contractile sheet protein (BMAA0439)

CENTER ID:	BumaA.18056.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.18056.a.B1.GE42206	full length		1	503

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.3938
UniProt:	A0A0H2WAY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSMQQLESSA EKVVVDQNNV NEDLKDILRR SFRPRTNEAA EAVQNAVETL LTYARRSRVV VREDVAQTIE QLVAELDKKI SEQLTLVLHN KRFQSLEGAW RGLHYLVSNT DTSENLKIRY LNISKADLGK TLRRFKGVVW DQSPIFKMIY EQEYGQFGGE PFGCLIGDFY FDHSMQDVSI LTEMSKISAA AHAPFIAAAA PGLLQMDDWS ELSNPRDVSK IFTATEYAFW RRLRESNDSR YLALTLPRFL ARVPYGPKTQ PVEEFGFEEK VDPNRAEDFC WANSAYAMGA NITRAFKTYG WCTKIRGVES GGAVEVLPKF VLPSQDREVD LHCPTEIAIS DRREHELSES GLMPLVYRKN SDTAAFIGAK TVHRPAIYED DDATANSNLS SRLPYIFATC RFAHYLKCIV RDKIGSFKSA EDTQRWLNDW LMNYVDGDPS ISSEVTKSQR PLSAAEVVVD EIPENPGYYR AQFFLRPHFQ LEGLTVSLRL VSKLPSTKHE VTT

NT Sequence

atgagtatgc agcagcttga atcgtctgcc gagaaggtgg tcgtcgatca gaacaacgtc aacgaggatc tgaaggacat cctgcgccgc tcgttccggc cgcgcaccaa cgaggcggcc gaggcggtgc agaacgcggt cgagacgctg ctcacgtatg cgcggcgcag ccgggtcgtg gttcgtgagg atgtcgcgca gacgatcgag cagctggttg ccgagctcga caagaagatc tccgagcaac tgacgctcgt gctgcacaac aagcgctttc agagcctcga aggcgcgtgg cgcggattgc actacctcgt gtcgaacacc gatacgagcg agaacctgaa gatccgctac ctgaacatct cgaaagcgga tctcggcaag acgctgcggc gcttcaaggg cgtcgtgtgg gatcagagtc cgattttcaa gatgatctac gagcaggagt acggccagtt cggcggcgag ccgttcggct gcctgatcgg cgacttctat ttcgaccaca gcatgcagga cgtgtcgatc ctcaccgaga tgtcgaagat ctcggcggcc gctcacgcgc cgttcatcgc ggctgccgcg cccggcctgc tgcagatgga cgactggagc gagctgtcga acccgcgcga cgtgtcgaag atcttcacgg cgaccgagta tgcgttctgg cggcgcctgc gcgagagcaa cgactcgcgc tacctcgcgc tcacgctgcc gcgcttcctc gcgcgtgtgc cgtatggtcc gaagacgcag cccgtcgaag agttcggctt cgaggagaag gtcgatccga accgcgcgga ggatttctgc tgggcgaact ccgcgtatgc gatgggcgcg aacatcacgc gcgcgttcaa gacctacggc tggtgcacga agattcgcgg cgtcgagtcg ggcggcgcgg tcgaggtgct gccgaagttc gtgctgccgt cgcaggatcg cgaagtcgat ctgcattgcc cgaccgagat cgcgatctcg gaccgccgcg agcacgaatt gtccgagtcg ggcctgatgc cgctcgtgta tcggaagaat tcggatacgg ccgcgttcat cggcgcgaag accgtgcatc gtccggcgat ctacgaggac gacgacgcga ccgcgaactc gaacctgtcg tcgcggctgc cgtacatctt cgcgacctgc cgtttcgcgc attacctgaa gtgcatcgtg cgcgacaaga tcggttcgtt caagtcggcc gaggacacgc agcgctggct caacgactgg ctgatgaact acgtggacgg cgacccgagc atctcgagcg aggtgacgaa gtcgcagcgt ccgctgtcgg cggcggaggt ggtcgtcgac gagattccgg aaaaccccgg ctactatcgc gcgcagttct tcctgcgccc ccatttccaa ctggagggtt tgaccgtttc tctccggctg gtttcgaagc ttccgtcgac caagcacgag gtgacgacct ga

Details for BumaA.18056.a.B1.GE42206

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	58kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS MQQLESSAEK VVVDQNNVNE DLKDILRRSF RPRTNEAAEA VQNAVETLLT YARRSRVVVR EDVAQTIEQL VAELDKKISE QLTLVLHNKR FQSLEGAWRG LHYLVSNTDT SENLKIRYLN ISKADLGKTL RRFKGVVWDQ SPIFKMIYEQ EYGQFGGEPF GCLIGDFYFD HSMQDVSILT EMSKISAAAH APFIAAAAPG LLQMDDWSEL SNPRDVSKIF TATEYAFWRR LRESNDSRYL ALTLPRFLAR VPYGPKTQPV EEFGFEEKVD PNRAEDFCWA NSAYAMGANI TRAFKTYGWC TKIRGVESGG AVEVLPKFVL PSQDREVDLH CPTEIAISDR REHELSESGL MPLVYRKNSD TAAFIGAKTV HRPAIYEDDD ATANSNLSSR LPYIFATCRF AHYLKCIVRD KIGSFKSAED TQRWLNDWLM NYVDGDPSIS SEVTKSQRPL SAAEVVVDEI PENPGYYRAQ FFLRPHFQLE GLTVSLRLVS KLP

Validated NT Sequence

cgacggaagc ttcgaaacca gccggagaga aacggtcaaa ccctccagtt ggaaatgggg gcgcaggaag aactgcgcgc gatagtagcc ggggttttcc ggaatctcgt cgacgaccac ctccgccgcc gacagcggac gctgcgactt cgtcacctcg ctcgagatgc tcgggtcgcc gtccacgtag ttcatcagcc agtcgttgag ccagcgctgc gtgtcctcgg ccgacttgaa cgaaccgatc ttgtcgcgca cgatgcactt caggtaatgc gcgaaacggc aggtcgcgaa gatgtacggc agccgcgacg acaggttcga gttcgcggtc gcgtcgtcgt cctcgtagat cgccggacga tgcacggtct tcgcgccgat gaacgcggcc gtatccgaat tcttccgata cacgagcggc atcaggcccg actcggacaa ttcgtgctcg cggcggtccg agatcgcgat ctcggtcggg caatgcagat cgacttcgcg atcctgcgac ggcagcacga acttcggcag cacctcgacc gcgccgcccg actcgacgcc gcgaatcttc gtgcaccagc cgtaggtctt gaacgcgcgc gtgatgttcg cgcccatcgc atacgcggag ttcgcccagc agaaatcctc cgcgcggttc ggatcgacct tctcctcgaa gccgaactct tcgacgggct gcgtcttcgg accatacggc acacgcgcga ggaagcgcgg cagcgtgagc gcgaggtagc gcgagtcgtt gctctcgcgc aggcgccgcc agaacgcata ctcggtcgcc gtgaagatct tcgacacgtc gcgcgggttc gacagctcgc tccagtcgtc catctgcagc aggccgggcg cggcagccgc gatgaacggc gcgtgagcgg ccgccgagat cttcgacatc tcggtgagga tcgacacgtc ctgcatgctg tggtcgaaat agaagtcgcc gatcaggcag ccgaacggct cgccgccgaa ctggccgtac tcctgctcgt agatcatctt gaaaatcgga ctctgatccc acacgacgcc cttgaagcgc cgcagcgtct tgccgagatc cgctttcgag atgttcaggt agcggatctt caggttctcg ctcgtatcgg tgttcgacac gaggtagtgc aatccgcgcc acgcgccttc gaggctctga aagcgcttgt tgtgcagcac gagcgtcagt tgctcggaga tcttcttgtc gagctcggca accagctgct cgatcgtctg cgcgacatcc tcacgaacca cgacccggct gcgccgcgca tacgtgagca gcgtctcgac cgcgttctgc accgcctcgg ccgcctcgtt ggtgcgcggc cggaacgagc ggcgcaggat gtccttcaga tcctcgttga cgttgttctg atcgacgacc accttctcgg cagacgattc aagctgctgc atactcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta tgcagcagct tgaatcgtct gccgagaagg tggtcgtcga tcagaacaac gtcaacgagg atctgaagga catcctgcgc cgctcgttcc ggccgcgcac caacgaggcg gccgaggcgg tgcagaacgc ggtcgagacg ctgctcacgt atgcgcggcg cagccgggtc gtggttcgtg aggatgtcgc gcagacgatc gagcagctgg ttgccgagct cgacaagaag atctccgagc aactgacgct cgtgctgcac aacaagcgct ttcagagcct cgaaggcgcg tggcgcggat tgcactacct cgtgtcgaac accgatacga gcgagaacct gaagatccgc tacctgaaca tctcgaaagc ggatctcggc aagacgctgc ggcgcttcaa gggcgtcgtg tgggatcaga gtccgatttt caagatgatc tacgagcagg agtacggcca gttcggcggc gagccgttcg gctgcctgat cggcgacttc tatttcgacc acagcatgca ggacgtgtcg atcctcaccg agatgtcgaa gatctcggcg gccgctcacg cgccgttcat cgcggctgcc gcgcccggcc tgctgcagat ggacgactgg agcgagctgt cgaacccgcg cgacgtgtcg aagatcttca cggcgaccga gtatgcgttc tggcggcgcc tgcgcgagag caacgactcg cgctacctcg cgctcacgct gccgcgcttc ctcgcgcgtg tgccgtatgg tccgaagacg cagcccgtcg aagagttcgg cttcgaggag aaggtcgatc cgaaccgcgc ggaggatttc tgctgggcga actccgcgta tgcgatgggc gcgaacatca cgcgcgcgtt caagacctac ggctggtgca cgaagattcg cggcgtcgag tcgggcggcg cggtcgaggt gctgccgaag ttcgtgctgc cgtcgcagga tcgcgaagtc gatctgcatt gcccgaccga gatcgcgatc tcggaccgcc gcgagcacga attgtccgag tcgggcctga tgccgctcgt gtatcggaag aattcggata cggccgcgtt catcggcgcg aagaccgtgc atcgtccggc gatctacgag gacgacgacg cgaccgcgaa ctcgaacctg tcgtcgcggc tgccgtacat cttcgcgacc tgccgtttcg cgcattacct gaagtgcatc gtgcgcgaca agatcggttc gttcaagtcg gccgaggaca cgcagcgctg gctcaacgac tggctgatga actacgtgga cggcgacccg agcatctcga gcgaggtgac gaagtcgcag cgtccgctgt cggcggcgga ggtggtcgtc gacgagattc cggaaaaccc cggctactat cgcgcgcagt tcttcctgcg cccccatttc caactggagg gtttgaccgt ttctctccgg ctggtttcga agcttccgtc gaccaagcac gaggtgacga cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat