BumaA.10014.c

Methionine import ATP-binding protein MetN 2 (EC 3.6.3.-) (metN2 BMAA1439)

CENTER ID: BumaA.10014.c
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.10014.c.B1.GE42203 full length 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.5009
UniProt: Q62B84

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQLLDSPGF IERSAAVPHK AAAAPATRDA AAPAAVPGAA VSFELVGKVF DGPRGPAAAL REVTLDIARG GVFGVIGRSG AGKSTLLRLV NGLERPTSGA VRVNGVDVGT LDERGLVALR RRIGMVFQHF NLLSAKTVAQ NIGLPLKIAG VPKAERARKV DALLDLVGLA AKRDAYPASL SGGQKQRVGI ARALVHDPAL LLCDEATSAL DPETTQSILA LLADINRRLG LTIMLITHEM EVIRAVCDTV AVVEQGEVVE TGPVWRVFGD PRHGATRALL RTLAHDLPAD LAAHVRPLDG AAPLPCGAQL LLDVRYTGAS GGEPDLGALT AALARNVGDA VHFVHGGLDR IQGRVQGRLV IAASLAARGA AGPDRIAAAL AAARRHANRV EVLGYV
NT Sequence
atggcgcagc tactcgattc acccggtttc atcgaacgct cggccgccgt gccgcacaag gccgcggccg cgccggctac gcgcgacgcg gccgccccag ccgccgtgcc cggcgcggcc gtgtcattcg agctcgtcgg caaggtgttc gacggcccgc gcggcccggc ggccgcgctg cgcgaagtga cgctcgacat cgcgcgcggc ggcgtgttcg gcgtgatcgg gcgcagcggc gcgggcaagt cgacgctgct gcggctcgtg aacgggctcg agcggccgac gtcgggcgcg gtgcgcgtga acggcgtcga cgtcggcacg ctcgacgagc gcgggctcgt cgcgctgcgg cgccggatcg gcatggtgtt tcagcatttc aacctgctgt ccgccaagac cgtcgcgcag aacatcgggc tgccgttgaa gatcgccggc gtgccgaagg ccgagcgtgc gcgcaaggtc gatgcgctgc tcgatctcgt cgggctcgcg gcgaaacgcg acgcgtatcc ggcgagcctg tcgggcggcc agaagcagcg cgtcggcatc gcgcgcgcgc tcgtccacga tccggcgctg ctgttgtgcg acgaggcgac gtccgcgctc gatccggaga cgacgcagtc gattctcgcg ctgctcgccg acatcaaccg gcggctcggc ctcacgatca tgctgatcac gcacgagatg gaagtgatcc gcgcggtgtg cgacacggtg gccgtcgtcg agcagggcga ggtggtcgag acggggccgg tgtggcgcgt gttcggcgat ccgcggcacg gcgcgacgcg cgcgctgttg cgcacgctcg cgcacgacct gccggccgat ctcgcggcgc acgtgcggcc gctcgacggc gcggcgccgc tgccgtgcgg cgcgcaactg ctgctcgacg tgcgctacac gggcgcgagc ggcggcgagc cggatctcgg tgcgctgacg gccgcgctcg cgcgcaacgt gggcgacgcg gtgcatttcg tgcacggcgg gctcgatcgg atccaggggc gcgtgcaggg gcggctcgtg atcgccgcgt cgctggccgc gcgcggcgcg gccggccccg accggatcgc ggccgcgctc gccgccgcgc gccggcatgc gaatcgcgtg gaggtactcg gctatgtctg a
Details for BumaA.10014.c.B1.GE42203
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 42kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 92
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA HLLDSPGFIE RSAAVPHKXA AAPATRDAAA PAAVPGAAVS FXLVGKVFDG PRGPAAALRE VTLDIARGGV FGVIGRXGAG KSTLLRXVNG LERXTSGAVR VNGVXVGTLD ERGLVALRRR IGMVFXHFNL LSAKTVAXNI GLPLKIAGLP KAXRARXVYA LLXLVWLAAK RDAYPAILSG XQKQRVXIAR ALVXDPXLLL CDEATSALDP ETXQSILALL ADINRRXGLT IMLITXEMEV IRAVCDTVAV VEQGEVVETG PVXRVFGDPR HGATRALXRT LAHDLPADLA AHVRPLXGAA PLPSGAQLLL DVRYTDASGG EPDLGALTAA LARNVGDAVH FVHGGLDRIQ GRVQGRLVIA ASLAARGAAG PDRIAAALAA ARRHAXRXXV LGYV
Validated NT Sequence
atggctcacc accaccacca ccatatggcg catctactcg attcacccgg tttcatcgaa cgctcggccg ccgtgccgca caaggncgcg gccgcgccgg ctacgcgcga cgcggccgcc ccagccgccg tgcccggcgc ggccgtgtca ttcnagctcg tcggcaaggt gttcgacggc ccgcgcggcc cggcggccgc gctgcgcgaa gtgacgctcg acatcgcgcg cggcggcgtg ttcggngtga tcgggcgcan cggcgcgggc aagtcgacgc tgctgcgghn cgtgaacggg ctcgagcggn cnacgtcggg cgcggtgcgc gtgaacggcg tcnacgtcgg cacgctcgac gagcgcgggc tcgtcgcgct gcggcgccgg atcggcatgg tgtttcanca tttcaacctg ctgtccgcca agaccgtcgc gcanaacatc gggctgccgt tgaagattgc cggcttgccg aaggccganc gtgcgcgcna ggtctatgcg ctgctcnatc tcgtctggct cgcggcgaaa cgcgacgcgt atccggcgat cctgtcgggc gnccagaagc agcgcgtcgr catcgcgcgc gcgctcgtcc amgatccgkc gctgctgttg tgcgaygagg cgacgtccgc gctcgatccg gagacgaygc agtcgattct cgcgctgctc gcsgacatca atcggcggnt cggcctcacg atcatgctga tcacgcncga gatggaagtg atccgcgcgg tgtgcgacac ggtggccgtc gtcgagcagg gcgaggtggt cgagacgggg ccggtgnggc gcgtgttcgg cgatccgcgg cacggcgcga cgcgcgcgct gntgcgcacg ctcgcgcacg acctgccggc cgatctcgcg gcgcacgtgc ggccgctngn cggcgcggcg ccgctgccga gcggcgcgca actgctgctc gacgtgcgct acacggacgc gagcggcggc gagccggatc tcggtgcgct gacggccgcg ctcgcgcgca acgtgggcga cgcggtgcat ttcgtgcacg gcgggctcga tcggatccag gggcgcgtgc aggggcggct cgtgatcgcc gcgtcgctgg ccgcgcgcgg cgcggccggc cccgaccgga tcgcggccgc gctcgccgcc gcgcgccggc atgcgnntcg nnnnnaggta ctcggctatg tctaa
Expected Protein Sequence
MAHHHHHHMA QLLDSPGFIE RSAAVPHKAA AAPATRDAAA PAAVPGAAVS FELVGKVFDG PRGPAAALRE VTLDIARGGV FGVIGRSGAG KSTLLRLVNG LERPTSGAVR VNGVDVGTLD ERGLVALRRR IGMVFQHFNL LSAKTVAQNI GLPLKIAGVP KAERARKVDA LLDLVGLAAK RDAYPASLSG GQKQRVGIAR ALVHDPALLL CDEATSALDP ETTQSILALL ADINRRLGLT IMLITHEMEV IRAVCDTVAV VEQGEVVETG PVWRVFGDPR HGATRALLRT LAHDLPADLA AHVRPLDGAA PLPCGAQLLL DVRYTGASGG EPDLGALTAA LARNVGDAVH FVHGGLDRIQ GRVQGRLVIA ASLAARGAAG PDRIAAALAA ARRHANRVEV LGYV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc agctactcga ttcacccggt ttcatcgaac gctcggccgc cgtgccgcac aaggccgcgg ccgcgccggc tacgcgcgac gcggccgccc cagccgccgt gcccggcgcg gccgtgtcat tcgagctcgt cggcaaggtg ttcgacggcc cgcgcggccc ggcggccgcg ctgcgcgaag tgacgctcga catcgcgcgc ggcggcgtgt tcggcgtgat cgggcgcagc ggcgcgggca agtcgacgct gctgcggctc gtgaacgggc tcgagcggcc gacgtcgggc gcggtgcgcg tgaacggcgt cgacgtcggc acgctcgacg agcgcgggct cgtcgcgctg cggcgccgga tcggcatggt gtttcagcat ttcaacctgc tgtccgccaa gaccgtcgcg cagaacatcg ggctgccgtt gaagatcgcc ggcgtgccga aggccgagcg tgcgcgcaag gtcgatgcgc tgctcgatct cgtcgggctc gcggcgaaac gcgacgcgta tccggcgagc ctgtcgggcg gccagaagca gcgcgtcggc atcgcgcgcg cgctcgtcca cgatccggcg ctgctgttgt gcgacgaggc gacgtccgcg ctcgatccgg agacgacgca gtcgattctc gcgctgctcg ccgacatcaa ccggcggctc ggcctcacga tcatgctgat cacgcacgag atggaagtga tccgcgcggt gtgcgacacg gtggccgtcg tcgagcaggg cgaggtggtc gagacggggc cggtgtggcg cgtgttcggc gatccgcggc acggcgcgac gcgcgcgctg ttgcgcacgc tcgcgcacga cctgccggcc gatctcgcgg cgcacgtgcg gccgctcgac ggcgcggcgc cgctgccgtg cggcgcgcaa ctgctgctcg acgtgcgcta cacgggcgcg agcggcggcg agccggatct cggtgcgctg acggccgcgc tcgcgcgcaa cgtgggcgac gcggtgcatt tcgtgcacgg cgggctcgat cggatccagg ggcgcgtgca ggggcggctc gtgatcgccg cgtcgctggc cgcgcgcggc gcggccggcc ccgaccggat cgcggccgcg ctcgccgccg cgcgccggca tgcgaatcgc gtggaggtac tcggctatgt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat