SSGCID: Target Page

BumaA.10014.b

ABC transporter, ATP-binding protein (BMAA1821)

CENTER ID:	BumaA.10014.b
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.10014.b.B1.GE42202	full length		1	347

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.5420
UniProt:	A0A0H2WA92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLEFDNVSF RYPGGAHGVD AVTLHAQPGE LLAVIGRSGS GKSTLLRLSA GLLDGYAGRI AIGGADMAGV PVWRREVGMV FQQYALFPHL SVADNVAYGL RMRGVAAVER RRRALEMLER VGLAAHAARS TTQLSGGQQQ RVALARALVI EPALLLLDEP LAALDAGIRH QLRDEIRALQ RACRATTLFV THDQDEALSM ADRVAIVDGG RVLQVGTPRS LYERPVSAQV ARFIGHSTIL RGRVQAGGTI DVRFATLCAD TGAHRPGAEV DVLVRPEHVE PDPPAQCVNR IEGALGEVRY FGATHRYDFL PHGAAHPLLC EGRVLAARGV AIEPRHLLVL PASGGAR

NT Sequence

atgagtctcg aattcgacaa cgtcagtttc agatatccgg gcggggcgca cggcgtcgac gccgtcacgc tgcacgcgca gccgggcgag ctgctcgccg tgatcggccg cagcggttcc ggcaagtcga cgctgctgcg gctgagcgcg ggcctcctcg acggctacgc cgggcgcatc gcgatcggcg gcgccgacat ggcgggcgtg ccggtgtggc ggcgcgaggt cggcatggtg tttcagcagt acgcattgtt tccgcatctg tccgtcgccg acaacgtcgc gtacgggctg cgcatgcgcg gcgtcgcagc cgtcgagcgc cgccggcgcg cgctcgagat gctcgagcgc gtcggcctcg ccgcgcatgc ggcgcgcagc acgacgcaac tgtcgggcgg ccagcagcag cgggtcgcgc ttgcccgcgc gctcgtgatc gagccggcgc tgctgctgct cgacgagccg ctcgccgcgc tcgacgcggg cattcggcat caattgcgcg acgagatccg cgcgctgcag cgcgcatgcc gcgcgacgac gctcttcgtc acgcacgatc aggacgaggc gctcagcatg gccgatcgcg tcgcgatcgt cgacggcggg cgcgtgctgc aagtgggcac gccgcgcagc ctgtacgagc ggccggtatc cgcgcaagtc gcgcgcttca tcggccactc gacgattctg cgtggccgcg tgcaagcggg cggcacgatc gacgtgcgct tcgcgacgct atgcgccgac acgggcgcgc atcggccggg cgcggaagtc gacgtgctgg tgcgccccga gcacgtggag cccgatccgc ccgcgcagtg cgtgaatcgc atcgagggcg cgctcggcga agtccgctac ttcggcgcga cgcaccgcta cgacttcttg ccgcatggcg cggcgcatcc gctgctctgc gagggccgcg tgctcgccgc gcgcggcgtc gcgatcgagc cgcgccatct gctcgtgctg cccgcttcgg gcggcgcgcg atga

Details for BumaA.10014.b.B1.GE42202

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	38kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS LEFDNVSFRY PGGAHGVDAV TLHAQPGELL AVIGRSGSGK STLLRLSAGL LDGYAGRIAI GGADMAGVPV WRREVGMVFQ QYALFPHLSV ADNVAYGLRM RGVAAVERRR RALEMLERVG LAAHAARSTT QLSGGQQQRV ALARALVIEP ALLLLDEPLA ALDAGIRHQL RDEIRALQRA CRATTLFVTH DQDEALSMAD RVAIVDGGRV LQVGTPRSLY ERPVSAQVAR FIGHSTILRG RVQAGGTIDV RFATLCADTG AHRPGAEVDV LVRPEHVEPD PPAQCVNRIE GALGEVRYFG ATHRYDFLPH GAAHPLLCEG RVLAARGVAI EPRHL

Validated NT Sequence

gagcagatgg cgcggctcga tcgcgacgcc gcgcgcggcg agcacgcggc cctcgcagag cagcggatgc gccgcgccat gcggcaagaa gtcgtagcgg tgcgtcgcgc cgaagtagcg gacttcgccg agcgcgccct cgatgcgatt cacgcactgc gcgggcggat cgggctccac gtgctcgggg cgcaccagca cgtcgacttc cgcgcccggc cgatgcgcgc ccgtgtcggc gcatagcgtc gcgaagcgca cgtcgatcgt gccgcccgct tgcacgcggc cacgcagaat cgtcgagtgg ccgatgaagc gcgcgacttg cgcggatacc ggccgctcgt acaggctgcg cggcgtgccc acttgcagca cgcgcccgcc gtcgacgatc gcgacgcgat cggccatgct gagcgcctcg tcctgatcgt gcgtgacgaa gagcgtcgtc gcgcggcatg cgcgctgcag cgcgcggatc tcgtcgcgca attgatgccg aatgcccgcg tcgagcgcgg cgagcggctc gtcgagcagc agcagcgccg gctcgatcac gagcgcgcgg gcaagcgcga cccgctgctg ctggccgccc gacagttgcg tcgtgctgcg cgccgcatgc gcggcgaggc cgacgcgctc gagcatctcg agcgcgcgcc ggcggcgctc gacggctgcg acgccgcgca tgcgcagccc gtacgcgacg ttgtcggcga cggacagatg cggaaacaat gcgtactgct gaaacaccat gccgacctcg cgccgccaca ccggcacgcc cgccatgtcg gcgccgccga tcgcgatgcg cccggcgtag ccgtcgagga ggcccgcgct cagccgcagc agcgtcgact tgccggaacc gctgcggccg atcacggcga gcagctcgcc cggctgcgcg tgcagcgtga cggcgtcgac gccgtgcgcc ccgcccggat atctgaaact gacgttgtcg aattcgagac tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc tcgaattcga caacgtcagt ttcagatatc cgggcggggc gcacggcgtc gacgccgtca cgctgcacgc gcagccgggc gagctgctcg ccgtgatcgg ccgcagcggt tccggcaagt cgacgctgct gcggctgagc gcgggcctcc tcgacggcta cgccgggcgc atcgcgatcg gcggcgccga catggcgggc gtgccggtgt ggcggcgcga ggtcggcatg gtgtttcagc agtacgcatt gtttccgcat ctgtccgtcg ccgacaacgt cgcgtacggg ctgcgcatgc gcggcgtcgc agccgtcgag cgccgccggc gcgcgctcga gatgctcgag cgcgtcggcc tcgccgcgca tgcggcgcgc agcacgacgc aactgtcggg cggccagcag cagcgggtcg cgcttgcccg cgcgctcgtg atcgagccgg cgctgctgct gctcgacgag ccgctcgccg cgctcgacgc gggcattcgg catcaattgc gcgacgagat ccgcgcgctg cagcgcgcat gccgcgcgac gacgctcttc gtcacgcacg atcaggacga ggcgctcagc atggccgatc gcgtcgcgat cgtcgacggc gggcgcgtgc tgcaagtggg cacgccgcgc agcctgtacg agcggccggt atccgcgcaa gtcgcgcgct tcatcggcca ctcgacgatt ctgcgtggcc gcgtgcaagc gggcggcacg atcgacgtgc gcttcgcgac gctatgcgcc gacacgggcg cgcatcggcc gggcgcggaa gtcgacgtgc tggtgcgccc cgagcacgtg gagcccgatc cgcccgcgca gtgcgtgaat cgcatcgagg gcgcgctcgg cgaagtccgc tacttcggcg cgacgcaccg ctacgacttc ttgccgcatg gcgcggcgca tccgctgctc tgcgagggcc gcgtgctcgc cgcgcgcggc gtcgcgatcg agccgcgcca tctgctcgtg ctgcccgctt cgggcggcgc gcgatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat