BumaA.01673.b
RND efflux system, outer membrane lipoprotein, NodT family (BMAA1392)
| CENTER ID: | BumaA.01673.b |
| ORGANISM: | Burkholderia mallei ATCC 23344 |
| ASSOCIATED DISEASE: | Glanders |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumaA.01673.b.B2.GE42322 | mature protein | 28 | 619 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumaA.01673.b.B2.PW38489 | mature protein | 28 | 619 |
Structures
6U94
DEPOSITED: 9/6/2019
DETERMINATION: XRay
CLONE: BumaA.01673.b.B2.GE42322
PROTEIN: BumaA.01673.b.B2.PW38489
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|243160.12.peg.4956 |
| UniProt: | A0A0H2WCA9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHSPPFLSSP KRALPIALAL SLAGCSMEPA YQRPDAPMPS AYPNGPAYAT PGAPRANPGE PAAAELGWRN FLADAQLQQL VALALANNRD LRVATLDIDE ARALYRIQRA AQFPAIDASV GLTSQRMSPA LRAPGQSAAI NSYDASVGLT HFEIDLFGRV RSLSHAAQEQ YLATEEARRS VHISLVAEVA NTYLTLLADR ALLALAQDTL RSQQDAADMI HRGKQAGAMA QLDEHRADTQ VQTARVAAEQ YTRQIAQDEN ALAVLIGGPL PAGVSRAAPL GDRALLAEFP AGLPSTLLER RPDIMAAEHR LIAANANIGA ARAAFFPRIT LTGALGVASA SLAGLFSGGV AWLFVPQLTL PIFNAGSNQA NLDLATVRRD INVAGYEHTI QDAFREVADN LAARATYERE VKAQEAMIRD LAETKRLADM RFRNGVDDYF GVFDAQRQLF AAQQLLVTYK LAGLTSRVTL YKALGGGWVE SAGAAAAQPR TGQAAPMARP AATPPQTTRP APVARSQTVP PRTVQPPIAQ PATAQPQTAQ AAPVAQSQTI PLRTVQPPIA QSSTSQPQTA QAAPITQART TPLRTAQPPA ARQQAPQSAP AAQPEAVPPQ TAQPPIFQP
NT Sequence
atgcatagcc caccgtttct ttcatcgccc aagcgcgcgc tgccgatcgc gctcgcgctc agcctcgccg gttgctcgat ggagccggcc taccagcggc ccgatgcgcc gatgccgtcc gcgtatccga acggccccgc ctacgcgacg ccgggcgcgc cgcgcgcgaa cccgggcgag ccggcggcgg ccgagctcgg ctggcggaat ttcctcgccg acgcgcagtt gcagcagctc gtcgcgctcg cgctcgcgaa caaccgcgac ctgcgcgtcg cgacgctcga catcgacgaa gcgcgcgcgc tgtaccgcat ccagcgcgcc gcgcagtttc ccgcaatcga tgcgagcgtc ggactcacga gccagcgcat gagccccgcg ctgcgcgcgc cggggcagtc cgcggcgatc aacagctatg acgcgagcgt cggcctcacc catttcgaga tcgacctgtt cggccgcgtg cgcagcctga gccacgccgc gcaggagcaa tacctcgcga cagaggaggc gcggcgcagc gtccacatca gcctcgtcgc cgaagtcgcg aacacctacc tgacgctgct cgccgatcgc gcgctgctcg cgctcgcgca ggacacgctc aggagccagc aggacgccgc cgacatgatc caccgcggca agcaggcggg cgcgatggcg cagctcgacg agcatcgcgc ggacacgcag gtgcagaccg cacgcgtcgc cgccgagcaa tacacgcggc agatcgcgca ggacgagaac gcgcttgccg tgctgatcgg cgggccgctg ccggccggcg tgtcgcgcgc ggcgccgctc ggcgatcgcg cgctgctcgc ggagttcccg gcgggcctgc cgtcgacgct gctcgagcgc cgccccgaca tcatggccgc cgagcatcgg ctcatcgccg cgaacgcgaa tatcggcgcg gcgcgcgcgg cgttcttccc gaggatcacg ttgacgggcg cgctcggcgt ggcgagcgcg agcctcgcgg ggctgttctc cggcggcgtc gcgtggctgt tcgtgccgca gctcacgctg ccgatcttca acgcgggcag caatcaggcg aatctcgatc tcgcgacggt gcgccgcgac atcaacgtgg ccggctatga gcacacgatc caggacgcgt tccgcgaggt cgccgacaac ctggccgcac gcgcgaccta cgagcgggaa gtgaaagcgc aggaggcgat gatccgcgac ctggccgaga cgaagcggct ggccgacatg cgttttcgca acggcgtcga cgactacttc ggcgtgttcg atgcgcaacg ccagctgttc gccgcgcagc aactgctcgt cacctacaag cttgccgggc tgacgagccg cgtgacgcta tacaaggcgc tcggcggcgg ctgggtcgag tcggcgggcg cggcggccgc gcaaccgcgg acggggcagg ccgcgccgat ggctcgaccg gcagccactc caccccagac gacgcgaccc gcgccggttg ctcgatcgca gacggttcca ccgcgaacgg ttcagccccc gatcgcccaa ccggcaaccg cgcaaccgca aacggcgcaa gccgcgccgg ttgctcagtc gcagacgatt ccactgcgaa cggtccagcc tcctatcgcc caatcgtcaa cctctcaacc gcaaacggcg caagccgcgc cgatcactca agcgcggacg acaccactcc gaacggctca gccgcccgcc gctcgacaac aggcgccgca atccgctccg gcggctcaac cggaggcagt tccaccgcaa acggcccaac cgccgatttt tcagccgtaa
Details for BumaA.01673.b.B2.GE42322
| HARVESTED ON: | 4/13/2018 |
| SEQUENCED ON: | 4/18/2018 |
| EXPECTED MW: | 64kDa |
| OBSERVED MW: | 64kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHME PAYQRPDAPM PSAYPNGPAY ATPGAPRANP GEPAAAELGW RNFLADAQLQ QLVALALANN RDLRVATLDI DEARALYRIQ RAAQFPAIDA SVGLTSQRMS PALRAPGQSA AINSYDASVG LTHFEIDLFG RVRSLSHAAQ EQYLATEEAR RSVHISLVAE VANTYLTLLA DRALLALAQD TLRSQQDAAD MIHRGKQAGA MAQLDEHRAD TQVQTARVAA EQYTRQIAQD ENALAVLIGG PLPAGVSRAA PLGDRALLAE FPAGLPSTLL ERRPDILAAE HRLIAANANI GAARAAFFPR ITWTGALGVA SASLAGLFSG GVAWLFVPQL TLPIFNAGSN QANLDLATVR RDINVAGYEH TIQDAFREVA DNLAARATYE REVKAQEAMI RDLAETKRLA DMRFRNGVDD YFGVFDAQRQ LFAAQQLLVT YKLAGLTSRV TLYKALGGGW VESAGAAAAQ PRTGQAAPMA RPAATPPQTT RPAPVARSQT VPPRTVQPPI AQPATAQPQT AQAAPVAQSQ TIPLRTVQPP IAQSSTSQPQ TAQAAPITQA RTTPLRTAQP PAARQQAPQS APAAQPEAVP PQTAQPPIFQ P
Validated NT Sequence
ccnnntgcgg tggaactgcc tccggttgag ccgccggagc ggattgcggc gcctgttgtc gagcggcggg cggctgagcc gttcggagtg gtgtcgtccg cgcttgagtg atcggcgcgg cttgcgccgt ttgcggttga gaggttgacg attgggcgat aggaggctgg accgttcgca gtggaatcgt ctgcgactga gcaaccggcg cggcttgcgc cgtttgcggt tgcgcggttg ccggttgggc gatcgggggc tgaaccgttc gcggtggaac cgtctgcgat cgagcaaccg gcgcgggtcg cgtcgtctgg ggtggagtgg ctgccggtcg agccatcggc gcggcctgcc ccgtccgcgg ttgcgcggcc gccgcgcccg ccgactcgac ccagccgccg ccgagcgcct tgtatagcgt cacgcggctc gtcagcccgg caagcttgta ggtgacgagc agttgctgcg cggcgaacag ctggcgttgc gcatcgaaca cgccgaagta gtcgtcgacg ccgttgcgaa aacgcatgtc ggccagccgc ttcgtctcgg ccaggtcgcg gatcatcgcc tcctgcgctt tcacttcccg ctcgtaggtc gcgcgtgcgg ccaggttgtc ggcgacctcg cggaacgcgt cctggatcgt gtgctcatag ccggccacgt tgatgtcgcg gcgcaccgtc gcgagatcga gattcgcctg attgctgccc gcgttgaaga tcggcagcgt gagctgcggc acgaacagcc acgcgacgcc gccggagaan nnnnnngcga ggctcgcgct cgccacgccg agcgcgnnnn nnnannngat cctcgggaag annnnngcgc gcgccgcgcc gatattcgcg ttcgnnnnnn nnnnnngatg nnnnnnnnnn nnnnnnncgg gnnnnnnnnn nngcannnnn gacggcaggc cnnnnnngaa ctcnnnnagc agcgcgcgat cgccgagcgg cgccgcgcgc gacacgccgg ccnncagcgg cccgccgatc agcacggcaa gcgcgttctc gtcctgcgcg atctgccgcg tgtattgctc ggcggcgacg cgtgcggtct gcacctgcgt gtccgcgcga tgctcgtcga gctgcgccat cgcgcccgcc tgcttgccgc ggtggatcat gtcggcggcg tcctgctggc tcctgagcgt gtcctgcgcg agcgcgagca gcgcgcgatc ggcgagcagc gtcaggtagg tgttcgcgac ttcggcgacg aggctgatgt ggacgctgcg ccgcgcctcc tctgtcgcga ggtattgctc ctgcgcggcg tggctcaggc tgcgcacgcg gccgaacagg tcgatctcga aatgggtgag gccgacgctc gcgtcatagc tgttgatcgc cgcggactgc cccggcgcgc gcagcgcggg gctcatgcgc tggctcgtga gtccgacgct cgcatcgatt gcgggaaact gcgcggcgcg ctggatgcgg tacagcgcgc gcgcttcgtc gatgtcgagc gtcgcgacgc gcaggtcgcg gttgttcgcg agcgcgagcg cgacgagctg ctgcaactgc gcgtcggcga ggaaattccg ccagccgagc tcggccgccg ccggctcgcc cgggttcgcg cgcggcgcgc ccggcgtcgc gtaggcgggg ccgttcggat acgcggacgg catcggcgca tcgggccgct ggtaggccgg ctccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHEP AYQRPDAPMP SAYPNGPAYA TPGAPRANPG EPAAAELGWR NFLADAQLQQ LVALALANNR DLRVATLDID EARALYRIQR AAQFPAIDAS VGLTSQRMSP ALRAPGQSAA INSYDASVGL THFEIDLFGR VRSLSHAAQE QYLATEEARR SVHISLVAEV ANTYLTLLAD RALLALAQDT LRSQQDAADM IHRGKQAGAM AQLDEHRADT QVQTARVAAE QYTRQIAQDE NALAVLIGGP LPAGVSRAAP LGDRALLAEF PAGLPSTLLE RRPDIMAAEH RLIAANANIG AARAAFFPRI TLTGALGVAS ASLAGLFSGG VAWLFVPQLT LPIFNAGSNQ ANLDLATVRR DINVAGYEHT IQDAFREVAD NLAARATYER EVKAQEAMIR DLAETKRLAD MRFRNGVDDY FGVFDAQRQL FAAQQLLVTY KLAGLTSRVT LYKALGGGWV ESAGAAAAQP RTGQAAPMAR PAATPPQTTR PAPVARSQTV PPRTVQPPIA QPATAQPQTA QAAPVAQSQT IPLRTVQPPI AQSSTSQPQT AQAAPITQAR TTPLRTAQPP AARQQAPQSA PAAQPEAVPP QTAQPPIFQP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgagccgg cctaccagcg gcccgatgcg ccgatgccgt ccgcgtatcc gaacggcccc gcctacgcga cgccgggcgc gccgcgcgcg aacccgggcg agccggcggc ggccgagctc ggctggcgga atttcctcgc cgacgcgcag ttgcagcagc tcgtcgcgct cgcgctcgcg aacaaccgcg acctgcgcgt cgcgacgctc gacatcgacg aagcgcgcgc gctgtaccgc atccagcgcg ccgcgcagtt tcccgcaatc gatgcgagcg tcggactcac gagccagcgc atgagccccg cgctgcgcgc gccggggcag tccgcggcga tcaacagcta tgacgcgagc gtcggcctca cccatttcga gatcgacctg ttcggccgcg tgcgcagcct gagccacgcc gcgcaggagc aatacctcgc gacagaggag gcgcggcgca gcgtccacat cagcctcgtc gccgaagtcg cgaacaccta cctgacgctg ctcgccgatc gcgcgctgct cgcgctcgcg caggacacgc tcaggagcca gcaggacgcc gccgacatga tccaccgcgg caagcaggcg ggcgcgatgg cgcagctcga cgagcatcgc gcggacacgc aggtgcagac cgcacgcgtc gccgccgagc aatacacgcg gcagatcgcg caggacgaga acgcgcttgc cgtgctgatc ggcgggccgc tgccggccgg cgtgtcgcgc gcggcgccgc tcggcgatcg cgcgctgctc gcggagttcc cggcgggcct gccgtcgacg ctgctcgagc gccgccccga catcatggcc gccgagcatc ggctcatcgc cgcgaacgcg aatatcggcg cggcgcgcgc ggcgttcttc ccgaggatca cgttgacggg cgcgctcggc gtggcgagcg cgagcctcgc ggggctgttc tccggcggcg tcgcgtggct gttcgtgccg cagctcacgc tgccgatctt caacgcgggc agcaatcagg cgaatctcga tctcgcgacg gtgcgccgcg acatcaacgt ggccggctat gagcacacga tccaggacgc gttccgcgag gtcgccgaca acctggccgc acgcgcgacc tacgagcggg aagtgaaagc gcaggaggcg atgatccgcg acctggccga gacgaagcgg ctggccgaca tgcgttttcg caacggcgtc gacgactact tcggcgtgtt cgatgcgcaa cgccagctgt tcgccgcgca gcaactgctc gtcacctaca agcttgccgg gctgacgagc cgcgtgacgc tatacaaggc gctcggcggc ggctgggtcg agtcggcggg cgcggcggcc gcgcaaccgc ggacggggca ggccgcgccg atggctcgac cggcagccac tccaccccag acgacgcgac ccgcgccggt tgctcgatcg cagacggttc caccgcgaac ggttcagccc ccgatcgccc aaccggcaac cgcgcaaccg caaacggcgc aagccgcgcc ggttgctcag tcgcagacga ttccactgcg aacggtccag cctcctatcg cccaatcgtc aacctctcaa ccgcaaacgg cgcaagccgc gccgatcact caagcgcgga cgacaccact ccgaacggct cagccgcccg ccgctcgaca acaggcgccg caatccgctc cggcggctca accggaggca gttccaccgc aaacggccca accgccgatt tttcagccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BumaA.01673.b.B2.PW38489
| PURIFICATION DATe: | 6/4/2018 |
| CONCENTRATION: | 17.86mg/ml |
| OBSERVED MW: | 70kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME PAYQRPDAPM PSAYPNGPAY ATPGAPRANP GEPAAAELGW RNFLADAQLQ QLVALALANN RDLRVATLDI DEARALYRIQ RAAQFPAIDA SVGLTSQRMS PALRAPGQSA AINSYDASVG LTHFEIDLFG RVRSLSHAAQ EQYLATEEAR RSVHISLVAE VANTYLTLLA DRALLALAQD TLRSQQDAAD MIHRGKQAGA MAQLDEHRAD TQVQTARVAA EQYTRQIAQD ENALAVLIGG PLPAGVSRAA PLGDRALLAE FPAGLPSTLL ERRPDILAAE HRLIAANANI GAARAAFFPR ITWTGALGVA SASLAGLFSG GVAWLFVPQL TLPIFNAGSN QANLDLATVR RDINVAGYEH TIQDAFREVA DNLAARATYE REVKAQEAMI RDLAETKRLA DMRFRNGVDD YFGVFDAQRQ LFAAQQLLVT YKLAGLTSRV TLYKALGGGW VESAGAAAAQ PRTGQAAPMA RPAATPPQTT RPAPVARSQT VPPRTVQPPI AQPATAQPQT AQAAPVAQSQ TIPLRTVQPP IAQSSTSQPQ TAQAAPITQA RTTPLRTAQP PAARQQAPQS APAAQPEAVP PQTAQPPIFQ P
Validated NT Sequence
ccnnntgcgg tggaactgcc tccggttgag ccgccggagc ggattgcggc gcctgttgtc gagcggcggg cggctgagcc gttcggagtg gtgtcgtccg cgcttgagtg atcggcgcgg cttgcgccgt ttgcggttga gaggttgacg attgggcgat aggaggctgg accgttcgca gtggaatcgt ctgcgactga gcaaccggcg cggcttgcgc cgtttgcggt tgcgcggttg ccggttgggc gatcgggggc tgaaccgttc gcggtggaac cgtctgcgat cgagcaaccg gcgcgggtcg cgtcgtctgg ggtggagtgg ctgccggtcg agccatcggc gcggcctgcc ccgtccgcgg ttgcgcggcc gccgcgcccg ccgactcgac ccagccgccg ccgagcgcct tgtatagcgt cacgcggctc gtcagcccgg caagcttgta ggtgacgagc agttgctgcg cggcgaacag ctggcgttgc gcatcgaaca cgccgaagta gtcgtcgacg ccgttgcgaa aacgcatgtc ggccagccgc ttcgtctcgg ccaggtcgcg gatcatcgcc tcctgcgctt tcacttcccg ctcgtaggtc gcgcgtgcgg ccaggttgtc ggcgacctcg cggaacgcgt cctggatcgt gtgctcatag ccggccacgt tgatgtcgcg gcgcaccgtc gcgagatcga gattcgcctg attgctgccc gcgttgaaga tcggcagcgt gagctgcggc acgaacagcc acgcgacgcc gccggagaan nnnnnngcga ggctcgcgct cgccacgccg agcgcgnnnn nnnannngat cctcgggaag annnnngcgc gcgccgcgcc gatattcgcg ttcgnnnnnn nnnnnngatg nnnnnnnnnn nnnnnnncgg gnnnnnnnnn nngcannnnn gacggcaggc cnnnnnngaa ctcnnnnagc agcgcgcgat cgccgagcgg cgccgcgcgc gacacgccgg ccnncagcgg cccgccgatc agcacggcaa gcgcgttctc gtcctgcgcg atctgccgcg tgtattgctc ggcggcgacg cgtgcggtct gcacctgcgt gtccgcgcga tgctcgtcga gctgcgccat cgcgcccgcc tgcttgccgc ggtggatcat gtcggcggcg tcctgctggc tcctgagcgt gtcctgcgcg agcgcgagca gcgcgcgatc ggcgagcagc gtcaggtagg tgttcgcgac ttcggcgacg aggctgatgt ggacgctgcg ccgcgcctcc tctgtcgcga ggtattgctc ctgcgcggcg tggctcaggc tgcgcacgcg gccgaacagg tcgatctcga aatgggtgag gccgacgctc gcgtcatagc tgttgatcgc cgcggactgc cccggcgcgc gcagcgcggg gctcatgcgc tggctcgtga gtccgacgct cgcatcgatt gcgggaaact gcgcggcgcg ctggatgcgg tacagcgcgc gcgcttcgtc gatgtcgagc gtcgcgacgc gcaggtcgcg gttgttcgcg agcgcgagcg cgacgagctg ctgcaactgc gcgtcggcga ggaaattccg ccagccgagc tcggccgccg ccggctcgcc cgggttcgcg cgcggcgcgc ccggcgtcgc gtaggcgggg ccgttcggat acgcggacgg catcggcgca tcgggccgct ggtaggccgg ctccatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHEP AYQRPDAPMP SAYPNGPAYA TPGAPRANPG EPAAAELGWR NFLADAQLQQ LVALALANNR DLRVATLDID EARALYRIQR AAQFPAIDAS VGLTSQRMSP ALRAPGQSAA INSYDASVGL THFEIDLFGR VRSLSHAAQE QYLATEEARR SVHISLVAEV ANTYLTLLAD RALLALAQDT LRSQQDAADM IHRGKQAGAM AQLDEHRADT QVQTARVAAE QYTRQIAQDE NALAVLIGGP LPAGVSRAAP LGDRALLAEF PAGLPSTLLE RRPDIMAAEH RLIAANANIG AARAAFFPRI TLTGALGVAS ASLAGLFSGG VAWLFVPQLT LPIFNAGSNQ ANLDLATVRR DINVAGYEHT IQDAFREVAD NLAARATYER EVKAQEAMIR DLAETKRLAD MRFRNGVDDY FGVFDAQRQL FAAQQLLVTY KLAGLTSRVT LYKALGGGWV ESAGAAAAQP RTGQAAPMAR PAATPPQTTR PAPVARSQTV PPRTVQPPIA QPATAQPQTA QAAPVAQSQT IPLRTVQPPI AQSSTSQPQT AQAAPITQAR TTPLRTAQPP AARQQAPQSA PAAQPEAVPP QTAQPPIFQP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGAGCCGG CCTACCAGCG GCCCGATGCG CCGATGCCGT CCGCGTATCC GAACGGCCCC GCCTACGCGA CGCCGGGCGC GCCGCGCGCG AACCCGGGCG AGCCGGCGGC GGCCGAGCTC GGCTGGCGGA ATTTCCTCGC CGACGCGCAG TTGCAGCAGC TCGTCGCGCT CGCGCTCGCG AACAACCGCG ACCTGCGCGT CGCGACGCTC GACATCGACG AAGCGCGCGC GCTGTACCGC ATCCAGCGCG CCGCGCAGTT TCCCGCAATC GATGCGAGCG TCGGACTCAC GAGCCAGCGC ATGAGCCCCG CGCTGCGCGC GCCGGGGCAG TCCGCGGCGA TCAACAGCTA TGACGCGAGC GTCGGCCTCA CCCATTTCGA GATCGACCTG TTCGGCCGCG TGCGCAGCCT GAGCCACGCC GCGCAGGAGC AATACCTCGC GACAGAGGAG GCGCGGCGCA GCGTCCACAT CAGCCTCGTC GCCGAAGTCG CGAACACCTA CCTGACGCTG CTCGCCGATC GCGCGCTGCT CGCGCTCGCG CAGGACACGC TCAGGAGCCA GCAGGACGCC GCCGACATGA TCCACCGCGG CAAGCAGGCG GGCGCGATGG CGCAGCTCGA CGAGCATCGC GCGGACACGC AGGTGCAGAC CGCACGCGTC GCCGCCGAGC AATACACGCG GCAGATCGCG CAGGACGAGA ACGCGCTTGC CGTGCTGATC GGCGGGCCGC TGCCGGCCGG CGTGTCGCGC GCGGCGCCGC TCGGCGATCG CGCGCTGCTC GCGGAGTTCC CGGCGGGCCT GCCGTCGACG CTGCTCGAGC GCCGCCCCGA CATCATGGCC GCCGAGCATC GGCTCATCGC CGCGAACGCG AATATCGGCG CGGCGCGCGC GGCGTTCTTC CCGAGGATCA CGTTGACGGG CGCGCTCGGC GTGGCGAGCG CGAGCCTCGC GGGGCTGTTC TCCGGCGGCG TCGCGTGGCT GTTCGTGCCG CAGCTCACGC TGCCGATCTT CAACGCGGGC AGCAATCAGG CGAATCTCGA TCTCGCGACG GTGCGCCGCG ACATCAACGT GGCCGGCTAT GAGCACACGA TCCAGGACGC GTTCCGCGAG GTCGCCGACA ACCTGGCCGC ACGCGCGACC TACGAGCGGG AAGTGAAAGC GCAGGAGGCG ATGATCCGCG ACCTGGCCGA GACGAAGCGG CTGGCCGACA TGCGTTTTCG CAACGGCGTC GACGACTACT TCGGCGTGTT CGATGCGCAA CGCCAGCTGT TCGCCGCGCA GCAACTGCTC GTCACCTACA AGCTTGCCGG GCTGACGAGC CGCGTGACGC TATACAAGGC GCTCGGCGGC GGCTGGGTCG AGTCGGCGGG CGCGGCGGCC GCGCAACCGC GGACGGGGCA GGCCGCGCCG ATGGCTCGAC CGGCAGCCAC TCCACCCCAG ACGACGCGAC CCGCGCCGGT TGCTCGATCG CAGACGGTTC CACCGCGAAC GGTTCAGCCC CCGATCGCCC AACCGGCAAC CGCGCAACCG CAAACGGCGC AAGCCGCGCC GGTTGCTCAG TCGCAGACGA TTCCACTGCG AACGGTCCAG CCTCCTATCG CCCAATCGTC AACCTCTCAA CCGCAAACGG CGCAAGCCGC GCCGATCACT CAAGCGCGGA CGACACCACT CCGAACGGCT CAGCCGCCCG CCGCTCGACA ACAGGCGCCG CAATCCGCTC CGGCGGCTCA ACCGGAGGCA GTTCCACCGC AAACGGCCCA ACCGCCGATT TTTCAGCCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT