BumaA.01668.a

Sensor histidine kinase (BMAA0677)

CENTER ID: BumaA.01668.a
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.01668.a.B1.GE42321 full length 1 538
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.4181
UniProt: A0A0H2WD10

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRADPATATA TATANPNPNP TVAGRDSPYG AAAPRGRNPV CWTRFDVLAR RAAPAASRSI PPEPNRAMSE STVMQMSAAI VEQLNCGVFS VDHDMRILAW NGFMQYHTGR SRDDAIGQDL FALFPELPAR WLRKKLESVF VLGVAMYTSW EHRPYLFRFD HSRPITGTID AMRQNCSFVP LDDGRGGVAA VGVTIVDATD ICIAHEVLGN REKRLTDALA ELTARHAELS DLNRQLAHAH QQLLQSEKLA AIGQLAAGIA HEINNPVGFV LSNVNTLGGY LGALIAHAHA VERLVAERQP QLAPALAELA RGADLDYLCE DAPALVDESK EGLARVRKIV VDLRDFSRVD SVHQWEWVDI HHCIESTLNI VRNEVKYAAD LVREYASLPM VRCIPSQVNQ VVLNLVVNAA QSYSSMRADG GHGDVPRGTI TIRTGVDAGD CPRIWFEVID AGCGIAPENL KRIFDPFFTT KPVGKGTGLG LSVAYGIVSA HGGEIAVSSA FGAGTTFRVT LPVERTGEPQ PEAAAVSHST PVASGIAS
NT Sequence
ttgcgcgccg acccggctac cgctaccgct accgctaccg ccaaccccaa ccccaacccc accgtcgcgg gcagggattc tccgtatggg gccgccgcgc cgcgaggccg taatccggtg tgttggacgc ggtttgacgt gctcgcgcgc cgcgcggcac cagccgcgtc ccgttcgatc ccgcccgaac cgaaccgcgc catgtccgaa tccaccgtta tgcagatgtc cgcagcaatc gtcgagcagc tcaactgcgg cgtgttctcg gtcgaccacg acatgcgcat cctcgcgtgg aacggcttca tgcagtacca cacgggccgc tcgcgcgacg acgcgatcgg ccaggatctg ttcgcgctct ttccggagct gcccgcgcgc tggctgcgca agaagctgga gagcgtgttc gtgctcggcg tcgcgatgta cacgtcgtgg gagcaccgtc cctatttgtt ccgcttcgat cattcgcggc cgatcaccgg cacgatcgac gcgatgcggc agaactgcag cttcgtgccg ctcgacgatg ggcgcggcgg cgtcgccgcg gtgggcgtga cgatcgtcga cgccaccgat atctgcatcg cgcacgaagt gctgggcaac cgcgagaagc ggctgaccga cgcgctcgcc gagctgaccg cgcgccatgc cgagctgtcg gacctgaacc gccagctcgc gcatgcgcat cagcaactgc tgcagtcgga gaagctcgcg gcgatcggcc agctcgccgc cggcatcgcg cacgaaatca acaatccggt cggcttcgtg ctgtcgaacg tgaacacgct cggcggctat ctcggcgcgc tgatcgcgca tgcgcacgcg gtcgagcggc tcgtcgccga gcgccagccg cagctcgcgc cggcgctcgc cgaactcgcg cggggcgcgg atcttgacta tctgtgcgag gacgcgccgg cgctcgtcga cgaatcgaag gaagggctcg cgcgcgtgcg caagatcgtc gtcgatctgc gcgatttctc gcgcgtcgac agcgtgcacc agtgggaatg ggtcgacatc catcactgca tcgaatcgac gctgaacatc gtgcgcaacg aagtgaaata cgcggccgat ctcgtgcgcg agtacgcatc gctgccgatg gtccgctgca tcccgtcgca ggtcaaccag gtggtgctga acctcgtcgt caacgccgcg caatcgtatt cgagcatgcg cgcggacggc ggccacggcg acgtgccgcg cggcacgatc acgatccgca cgggcgtcga cgcgggcgac tgcccgcgca tctggttcga ggtcatcgat gcgggctgcg ggatcgcccc cgagaatctc aagcggatct tcgatccgtt cttcacgacg aagccggtcg gcaagggcac ggggctcggg ctgtcggtcg cctacggcat cgtcagcgcg cacggcggcg agatcgcggt gtcgagcgcg ttcggcgcgg gcacgacgtt tcgcgtgacg ctgcccgtcg agcgcaccgg cgagccgcag cccgaagcgg cggccgtttc ccattcgacg ccggttgcgt ccggcatcgc aagctga
Details for BumaA.01668.a.B1.GE42321
HARVESTED ON: 4/13/2018
SEQUENCED ON: 4/18/2018
EXPECTED MW: 59kDa
OBSERVED MW: 59kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML RADPATATAT ATANPNPNPT VAGRDSPYGA AAPRGRNPVC WTRFDVLARR AAPAASRSIP PEPNRAMSES TVMQMSAAIV EQLNCGVFSV DHDMRILAWN GFMQYHTGRS RDDAIGQDLF ALFPELPARW LRKKLESVFV LGVAMYTSWE HRPYLFRFDH SRPITGTIDA MRQNCSFVPL DDGRGGVAAV GVTIVDATDI CIAHEVLGNR EKRLTDALAE LTARHAELSD LNRQLAHAHQ QLLQSEKLAA IGQLAAGIAH EINNPVGFVL SNVNTLGGYL GALIAHAHAV ERLVAERQPQ LAPALAELAR GADLDYLCED APALVDESKE GLARVRKIVV DLRDFSRVDS VHQWEWVDIH HCIESTLNIV RNEVKYAADL VREYASLPMV RCIPSQVNQV VLNLVVNAAQ SYSSMRADGG HGDVPRGTIT IRTGVDAGDC PRIWFEVIDA GCGIAPENLK RIFDPFFTTK PVGKGTGLGL SVAYGIVSAH GGEIAVSSAF GAGTTFRVTL PVERTGEPQP EAAAVSHS
Validated NT Sequence
tcgaatggga aacggccgcc gcttcgggct gcggctcgcc ggtgcgctcg acgggcagcg tcacgcgaaa cgtcgtgccc gcgccgaacg cgctcgacac cgcgatctcg ccgccgtgcg cgctgacgat gccgtaggcg accgacagcc cgagccccgt gcccttgccg accggcttcg tcgtgaagaa cggatcgaag atccgcttga gattctcggg ggcgatcccg cagcccgcat cgatgacctc gaaccagatg cgcgggcagt cgcccgcgtc gacgcccgtg cggatcgtga tcgtgccgcg cggcacgtcg ccgtggccgc cgtccgcgcg catgctcgaa tacgattgcg cggcgttgac gacgaggttc agcaccacct ggttgacctg cgacgggatg cagcggacca tcggcagcga tgcgtactcg cgcacgagat cggccgcgta tttcacttcg ttgcgcacga tgttcagcgt cgattcgatg cagtgatgga tgtcgaccca ttcccactgg tgcacgctgt cgacgcgcga gaaatcgcgc agatcgacga cgatcttgcg cacgcgcgcg agcccttcct tcgattcgtc gacgagcgcc ggcgcgtcct cgcacagata gtcaagatcc gcgccccgcg cgagttcggc gagcgccggc gcgagctgcg gctggcgctc ggcgacgagc cgctcgaccg cgtgcgcatg cgcgatcagc gcgccgagat agccgccgag cgtgttcacg ttcgacagca cgaagccgac cggattgttg atttcgtgcg cgatgccggc ggcgagctgg ccgatcgccg cgagcttctc cgactgcagc agttgctgat gcgcatgcgc gagctggcgg ttcaggtccg acagctcggc atggcgcgcg gtcagctcgg cgagcgcgtc ggtcagccgc ttctcgcggt tgcccagcac ttcgtgcgcg atgcagatat cggtggcgtc gacgatcgtc acgcccaccg cggcgacgcc gccgcgccca tcgtcgagcg gcacgaagct gcagttctgc cgcatcgcgt cgatcgtgcc ggtgatcggc cgcgaatgat cgaagcggaa caaataggga cggtgctccc acgacgtgta catcgcgacg ccgagcacga acacgctctc cagcttcttg cgcagccagc gcgcgggcag ctccggaaag agcgcgaaca gatcctggcc gatcgcgtcg tcgcgcgagc ggcccgtgtg gtactgcatg aagccgttcc acgcgaggat gcgcatgtcg tggtcgaccg agaacacgcc gcagttgagc tgctcgacga ttgctgcgga catctgcata acggtggatt cggacatggc gcggttcggt tcgggcggga tcgaacggga cgcggctggt gccgcgcggc gcgcgagcac gtcaaaccgc gtccaacaca ccggattacg gcctcgcggc gcggcggccc catacggaga atccctgccc gcgacggtgg ggttggggtt ggggttggcg gtagcggtag cggtagcggt agccgggtcg gcgcgcaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMR ADPATATATA TANPNPNPTV AGRDSPYGAA APRGRNPVCW TRFDVLARRA APAASRSIPP EPNRAMSEST VMQMSAAIVE QLNCGVFSVD HDMRILAWNG FMQYHTGRSR DDAIGQDLFA LFPELPARWL RKKLESVFVL GVAMYTSWEH RPYLFRFDHS RPITGTIDAM RQNCSFVPLD DGRGGVAAVG VTIVDATDIC IAHEVLGNRE KRLTDALAEL TARHAELSDL NRQLAHAHQQ LLQSEKLAAI GQLAAGIAHE INNPVGFVLS NVNTLGGYLG ALIAHAHAVE RLVAERQPQL APALAELARG ADLDYLCEDA PALVDESKEG LARVRKIVVD LRDFSRVDSV HQWEWVDIHH CIESTLNIVR NEVKYAADLV REYASLPMVR CIPSQVNQVV LNLVVNAAQS YSSMRADGGH GDVPRGTITI RTGVDAGDCP RIWFEVIDAG CGIAPENLKR IFDPFFTTKP VGKGTGLGLS VAYGIVSAHG GEIAVSSAFG AGTTFRVTLP VERTGEPQPE AAAVSHSTPV ASGIAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcgcg ccgacccggc taccgctacc gctaccgcta ccgccaaccc caaccccaac cccaccgtcg cgggcaggga ttctccgtat ggggccgccg cgccgcgagg ccgtaatccg gtgtgttgga cgcggtttga cgtgctcgcg cgccgcgcgg caccagccgc gtcccgttcg atcccgcccg aaccgaaccg cgccatgtcc gaatccaccg ttatgcagat gtccgcagca atcgtcgagc agctcaactg cggcgtgttc tcggtcgacc acgacatgcg catcctcgcg tggaacggct tcatgcagta ccacacgggc cgctcgcgcg acgacgcgat cggccaggat ctgttcgcgc tctttccgga gctgcccgcg cgctggctgc gcaagaagct ggagagcgtg ttcgtgctcg gcgtcgcgat gtacacgtcg tgggagcacc gtccctattt gttccgcttc gatcattcgc ggccgatcac cggcacgatc gacgcgatgc ggcagaactg cagcttcgtg ccgctcgacg atgggcgcgg cggcgtcgcc gcggtgggcg tgacgatcgt cgacgccacc gatatctgca tcgcgcacga agtgctgggc aaccgcgaga agcggctgac cgacgcgctc gccgagctga ccgcgcgcca tgccgagctg tcggacctga accgccagct cgcgcatgcg catcagcaac tgctgcagtc ggagaagctc gcggcgatcg gccagctcgc cgccggcatc gcgcacgaaa tcaacaatcc ggtcggcttc gtgctgtcga acgtgaacac gctcggcggc tatctcggcg cgctgatcgc gcatgcgcac gcggtcgagc ggctcgtcgc cgagcgccag ccgcagctcg cgccggcgct cgccgaactc gcgcggggcg cggatcttga ctatctgtgc gaggacgcgc cggcgctcgt cgacgaatcg aaggaagggc tcgcgcgcgt gcgcaagatc gtcgtcgatc tgcgcgattt ctcgcgcgtc gacagcgtgc accagtggga atgggtcgac atccatcact gcatcgaatc gacgctgaac atcgtgcgca acgaagtgaa atacgcggcc gatctcgtgc gcgagtacgc atcgctgccg atggtccgct gcatcccgtc gcaggtcaac caggtggtgc tgaacctcgt cgtcaacgcc gcgcaatcgt attcgagcat gcgcgcggac ggcggccacg gcgacgtgcc gcgcggcacg atcacgatcc gcacgggcgt cgacgcgggc gactgcccgc gcatctggtt cgaggtcatc gatgcgggct gcgggatcgc ccccgagaat ctcaagcgga tcttcgatcc gttcttcacg acgaagccgg tcggcaaggg cacggggctc gggctgtcgg tcgcctacgg catcgtcagc gcgcacggcg gcgagatcgc ggtgtcgagc gcgttcggcg cgggcacgac gtttcgcgtg acgctgcccg tcgagcgcac cggcgagccg cagcccgaag cggcggccgt ttcccattcg acgccggttg cgtccggcat cgcaagctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at