BumaA.01563.a
4-hydroxy-tetrahydrodipicolinate synthase (HTPA synthase) (EC 4.3.3.7) (dapA BMA1678)
| CENTER ID: | BumaA.01563.a |
| ORGANISM: | Burkholderia mallei ATCC 23344 |
| ASSOCIATED DISEASE: | Glanders |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumaA.01563.a.B1.GE42200 | full length | 1 | 300 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BumaA.01563.a.B1.PS38510 | full length | 1 | 300 |
Structures
6MQH
DEPOSITED: 10/9/2018
DETERMINATION: XRay
CLONE: BumaA.01563.a.B1.GE42200
PROTEIN: BumaA.01563.a.B1.PW38480
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|243160.12.peg.1715 |
| UniProt: | A0A0H2WFM6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANGTQDGIQ IRGSVPAIVT PMLEDGGLDL AAFRKLIDWH IEEGTDALVV VGTSGESATL SVDEHVLMIE TAVKHAAKRI PIVAGAGGNS TTEAIELSKH AKAVGADATL QVVPYYNKPT QEGIYRHFKA IAEAVDLPVI LYNVPGRTVA DMSNETTLRL AQVPGIIGVK DATGNIDRAA QLIKAAPAHF SIYSGDDPTA IALMLLGGHG NISVTANVAP RAMSELCRAA LAADVKTARE IHMKLLSLHK HLFIEANPIP VKWALQQMGK IAGGIRLPLT PLDERCHETV RGALREAGLL
NT Sequence
atggctaacg gcactcaaga cggcattcaa atccgcggca gcgtccccgc gatcgtcacc ccgatgctcg aggacggcgg cctcgatctg gcggccttcc gcaagctgat cgactggcat atcgaagagg ggacggatgc cctcgtcgtg gtcggcacga gcggcgaatc ggcgacgctt tccgtcgacg agcacgtgct catgatcgag accgcggtca agcacgcggc gaagcggatt ccgatcgtcg cgggcgcggg cggcaactcg acgaccgagg cgatcgaact gtcgaagcat gcgaaggcgg tgggcgccga tgcgacgctg caagtggtgc cgtactacaa caagccgacg caggaaggga tctaccgcca cttcaaggcg atcgccgagg cggtggacct tccggtgatc ctgtacaacg tgccgggccg cactgtcgcg gacatgtcga acgagacgac cctgcggctt gcgcaagtgc cgggcatcat cggcgtgaag gacgcgacgg gcaatatcga tcgcgccgcg caactgatca aggcggcgcc cgcgcacttc tcgatctaca gcggcgacga tccgaccgcg atcgcgctga tgctcctcgg cggccacggc aacatctcgg tgacggcgaa cgtcgcgccg cgcgcgatga gcgagctttg ccgcgcggcg cttgccgccg acgtgaagac ggcgcgcgag atccacatga agctcctgtc gctgcacaag catctgttca tcgaggcgaa cccgattccg gtgaagtggg cgctgcaaca gatgggcaag atcgcgggcg gcatccgcct gccgctcacg ccgctcgacg agcgctgcca tgaaaccgtg cgcggcgcgc ttcgcgaggc cggcctcctg tga
Details for BumaA.01563.a.B1.GE42200
| HARVESTED ON: | 2/23/2018 |
| SEQUENCED ON: | 3/1/2018 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA NGTQDGIQIR GSVPAIVTPM LEDGGLDLAA FRKLIDWHIE EGTDALVVVG TSGESATLSV DEHVLMIETA VKHAAKRIPI VAGAGGNSTT EAIELSKHAK AVGADATLQV VPYYNKPTQE GIYRHFKAIA EAVDLPVILY NVPGRTVADM SNETTLRLAQ VPGIIGVKDA TGNIDRAAQL IKAAPAHFSI YSGDDPTAIA LMLLGGHGNI SVTANVAPRA MSELCRAALA ADVKTAREIH MKLLSLHKHL FIEANPIPVK WALQQMGKIA GGIRLPLTPL DERCHETVRG AXXEAGLL
Validated NT Sequence
ttactcactt acaggaggcc ggcctcgcnn nnngcgccgc gcacggtttc atggcagcgc tcgtcgagcg gcgtgagcgg caggcggatg ccgcccgcga tcttgcccat ctgttgcagc gcccacttca ccggaatcgg gttcgcctcg atgaacagat gcttgtgcag cgacaggagc ttcatgtgga tctcgcgcgc cgtcttcacg tcggcggcaa gcgccgcgcg gcaaagctcg ctcatcgcgc gcggcgcgac gttcgccgtc accgagatgt tgccgtggcc gccgaggagc atcagcgcga tcgcggtcgg atcgtcgccg ctgtagatcg agaagtgcgc gggcgccgcc ttgatcagtt gcgcggcgcg atcgatattg cccgtcgcgt ccttcacgcc gatgatgccc ggcacttgcg caagccgcag ggtcgtctcg ttcgacatgt ccgcgacagt gcggcccggc acgttgtaca ggatcaccgg aaggtccacc gcctcggcga tcgccttgaa gtggcggtag atcccttcct gcgtcggctt gttgtagtac ggcaccactt gcagcgtcgc atcggcgccc accgccttcg catgcttcga cagttcgatc gcctcggtcg tcgagttgcc gcccgcgccc gcgacgatcg gaatccgctt cgccgcgtgc ttgaccgcgg tctcgatcat gagcacgtgc tcgtcgacgg aaagcgtcgc cgattcgccg ctcgtgccga ccacgacgag ggcatccgtc ccctcttcga tatgccagtc gatcagcttg cggaaggccg ccagatcgag gccgccgtcc tcgagcatcg gggtgacgat cgcggggacg ctgccgcgga tttgaatgcc gtcttgagtg ccgttagcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA NGTQDGIQIR GSVPAIVTPM LEDGGLDLAA FRKLIDWHIE EGTDALVVVG TSGESATLSV DEHVLMIETA VKHAAKRIPI VAGAGGNSTT EAIELSKHAK AVGADATLQV VPYYNKPTQE GIYRHFKAIA EAVDLPVILY NVPGRTVADM SNETTLRLAQ VPGIIGVKDA TGNIDRAAQL IKAAPAHFSI YSGDDPTAIA LMLLGGHGNI SVTANVAPRA MSELCRAALA ADVKTAREIH MKLLSLHKHL FIEANPIPVK WALQQMGKIA GGIRLPLTPL DERCHETVRG ALREAGLL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta acggcactca agacggcatt caaatccgcg gcagcgtccc cgcgatcgtc accccgatgc tcgaggacgg cggcctcgat ctggcggcct tccgcaagct gatcgactgg catatcgaag aggggacgga tgccctcgtc gtggtcggca cgagcggcga atcggcgacg ctttccgtcg acgagcacgt gctcatgatc gagaccgcgg tcaagcacgc ggcgaagcgg attccgatcg tcgcgggcgc gggcggcaac tcgacgaccg aggcgatcga actgtcgaag catgcgaagg cggtgggcgc cgatgcgacg ctgcaagtgg tgccgtacta caacaagccg acgcaggaag ggatctaccg ccacttcaag gcgatcgccg aggcggtgga ccttccggtg atcctgtaca acgtgccggg ccgcactgtc gcggacatgt cgaacgagac gaccctgcgg cttgcgcaag tgccgggcat catcggcgtg aaggacgcga cgggcaatat cgatcgcgcc gcgcaactga tcaaggcggc gcccgcgcac ttctcgatct acagcggcga cgatccgacc gcgatcgcgc tgatgctcct cggcggccac ggcaacatct cggtgacggc gaacgtcgcg ccgcgcgcga tgagcgagct ttgccgcgcg gcgcttgccg ccgacgtgaa gacggcgcgc gagatccaca tgaagctcct gtcgctgcac aagcatctgt tcatcgaggc gaacccgatt ccggtgaagt gggcgctgca acagatgggc aagatcgcgg gcggcatccg cctgccgctc acgccgctcg acgagcgctg ccatgaaacc gtgcgcggcg cgcttcgcga ggccggcctc ctgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BumaA.01563.a.B1.PS38510
| PURIFICATION DATe: | 9/25/2018 |
| CONCENTRATION: | 28.2mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA NGTQDGIQIR GSVPAIVTPM LEDGGLDLAA FRKLIDWHIE EGTDALVVVG TSGESATLSV DEHVLMIETA VKHAAKRIPI VAGAGGNSTT EAIELSKHAK AVGADATLQV VPYYNKPTQE GIYRHFKAIA EAVDLPVILY NVPGRTVADM SNETTLRLAQ VPGIIGVKDA TGNIDRAAQL IKAAPAHFSI YSGDDPTAIA LMLLGGHGNI SVTANVAPRA MSELCRAALA ADVKTAREIH MKLLSLHKHL FIEANPIPVK WALQQMGKIA GGIRLPLTPL DERCHETVRG AXXEAGLL
Validated NT Sequence
ttactcactt acaggaggcc ggcctcgcnn nnngcgccgc gcacggtttc atggcagcgc tcgtcgagcg gcgtgagcgg caggcggatg ccgcccgcga tcttgcccat ctgttgcagc gcccacttca ccggaatcgg gttcgcctcg atgaacagat gcttgtgcag cgacaggagc ttcatgtgga tctcgcgcgc cgtcttcacg tcggcggcaa gcgccgcgcg gcaaagctcg ctcatcgcgc gcggcgcgac gttcgccgtc accgagatgt tgccgtggcc gccgaggagc atcagcgcga tcgcggtcgg atcgtcgccg ctgtagatcg agaagtgcgc gggcgccgcc ttgatcagtt gcgcggcgcg atcgatattg cccgtcgcgt ccttcacgcc gatgatgccc ggcacttgcg caagccgcag ggtcgtctcg ttcgacatgt ccgcgacagt gcggcccggc acgttgtaca ggatcaccgg aaggtccacc gcctcggcga tcgccttgaa gtggcggtag atcccttcct gcgtcggctt gttgtagtac ggcaccactt gcagcgtcgc atcggcgccc accgccttcg catgcttcga cagttcgatc gcctcggtcg tcgagttgcc gcccgcgccc gcgacgatcg gaatccgctt cgccgcgtgc ttgaccgcgg tctcgatcat gagcacgtgc tcgtcgacgg aaagcgtcgc cgattcgccg ctcgtgccga ccacgacgag ggcatccgtc ccctcttcga tatgccagtc gatcagcttg cggaaggccg ccagatcgag gccgccgtcc tcgagcatcg gggtgacgat cgcggggacg ctgccgcgga tttgaatgcc gtcttgagtg ccgttagcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMA NGTQDGIQIR GSVPAIVTPM LEDGGLDLAA FRKLIDWHIE EGTDALVVVG TSGESATLSV DEHVLMIETA VKHAAKRIPI VAGAGGNSTT EAIELSKHAK AVGADATLQV VPYYNKPTQE GIYRHFKAIA EAVDLPVILY NVPGRTVADM SNETTLRLAQ VPGIIGVKDA TGNIDRAAQL IKAAPAHFSI YSGDDPTAIA LMLLGGHGNI SVTANVAPRA MSELCRAALA ADVKTAREIH MKLLSLHKHL FIEANPIPVK WALQQMGKIA GGIRLPLTPL DERCHETVRG ALREAGLL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTA ACGGCACTCA AGACGGCATT CAAATCCGCG GCAGCGTCCC CGCGATCGTC ACCCCGATGC TCGAGGACGG CGGCCTCGAT CTGGCGGCCT TCCGCAAGCT GATCGACTGG CATATCGAAG AGGGGACGGA TGCCCTCGTC GTGGTCGGCA CGAGCGGCGA ATCGGCGACG CTTTCCGTCG ACGAGCACGT GCTCATGATC GAGACCGCGG TCAAGCACGC GGCGAAGCGG ATTCCGATCG TCGCGGGCGC GGGCGGCAAC TCGACGACCG AGGCGATCGA ACTGTCGAAG CATGCGAAGG CGGTGGGCGC CGATGCGACG CTGCAAGTGG TGCCGTACTA CAACAAGCCG ACGCAGGAAG GGATCTACCG CCACTTCAAG GCGATCGCCG AGGCGGTGGA CCTTCCGGTG ATCCTGTACA ACGTGCCGGG CCGCACTGTC GCGGACATGT CGAACGAGAC GACCCTGCGG CTTGCGCAAG TGCCGGGCAT CATCGGCGTG AAGGACGCGA CGGGCAATAT CGATCGCGCC GCGCAACTGA TCAAGGCGGC GCCCGCGCAC TTCTCGATCT ACAGCGGCGA CGATCCGACC GCGATCGCGC TGATGCTCCT CGGCGGCCAC GGCAACATCT CGGTGACGGC GAACGTCGCG CCGCGCGCGA TGAGCGAGCT TTGCCGCGCG GCGCTTGCCG CCGACGTGAA GACGGCGCGC GAGATCCACA TGAAGCTCCT GTCGCTGCAC AAGCATCTGT TCATCGAGGC GAACCCGATT CCGGTGAAGT GGGCGCTGCA ACAGATGGGC AAGATCGCGG GCGGCATCCG CCTGCCGCTC ACGCCGCTCG ACGAGCGCTG CCATGAAACC GTGCGCGGCG CGCTTCGCGA GGCCGGCCTC CTGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT