SSGCID: Target Page

BumaA.00863.a

Sensory box histidine kinase (BMAA0908)

CENTER ID:	BumaA.00863.a
ORGANISM:	Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE:	Glanders
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BumaA.00863.a.B2.GE42199	PAS and kinase domains		211	711

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243160.12.peg.4424
UniProt:	A0A0H2WCZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASGIGRFGK RASRLIDFGL WAAAALLLLT AGVGALGTAR LSDNEADIHR ASEVISGLER LLALARDMET SQRGYILTGK TDYLQPYKAA LPEIRKQFDA LATLVQGDAV QQQRLATLRM LLEKKQHELA ATIELRKTEG FDAAQAIVSS DAGKIFMDRA RVMVNEMEAT VQQQLRTLRD RARATRDIAI GVGLASGLLT LAVCVSFGYV MRRLLLVEAA AAHDLFEQRE LLHVTLASIG DGVIATDVGG RITFFNRVAE EMTALCADAV VGQPIERVLM FRHATTQRVI DNPARVAVER RRTASLPAHT RFVGRSGGEL PVDGNAAPTF DANGKLVGAV LIVRDVSERE RAEERFRLAV EAAPTAMIMV GRDGRIVLVN SQAEKLFGYA RGDMIGHGID MLVPERFRAG HWAHRDAFFG NPDARPMGEG RDLFGLRRDG SEFPVEIGLN PITTSEGAFV LCSIADITAR KRAELELRRR SEELARSNQD LEQFAYVASH DLQEPLRAVA GPLQLLQRRY QGQLDARADE FIGHAVDGAT RMQSLIDDLL SYSRVGRLED ARRPVDCEHV LDEALRNLSA AIRESGAQVT HDPLPTVHGI EAQLVLLFQN LIGNAIKFRG KDRVARIHLE AHTRDDAWLL RVRDNGIGID PQYFERIFLI FQRLHTRREY PGTGLGLALC KRIVEHHGGR IFVESEPGQG TTFSFTLSRP GADETRWPRD PRE

NT Sequence

atggcgtcgg gcatcgggcg attcgggaaa agagcgtcgc gcttgatcga tttcggtttg tgggccgccg ccgcactgtt actgctgacg gcgggcgtcg gcgcgctcgg cacggcccgc ctgtccgaca acgaggcgga tatccatcgc gcgagcgagg tgatatcggg tctcgaacgg ctgctggcgc tggcccgcga catggagacg agccagcgcg gatatatcct caccggcaag acggactacc ttcagcctta caaggcggca ctgcccgaaa tccgcaagca gttcgacgcg ctcgcgacgc tcgtgcaggg cgatgccgtt cagcagcagc ggctcgcgac gctgcgcatg ctgctcgaga aaaagcagca cgagctcgcg gcgacgatcg agctgcgcaa gacggagggg ttcgacgcgg cgcaggcgat cgtctcgagc gatgcgggca agatcttcat ggaccgcgcg cgcgtgatgg tcaacgaaat ggaggcgacc gtccagcagc aactgaggac gctgcgcgat cgcgccaggg cgacacgcga catcgcgatc ggcgtggggc tcgcgtccgg gctgctcacg ctcgccgtgt gcgtatcgtt cggctatgtg atgcgccgcc tgctgctcgt cgaggcggcc gcggcccacg atctgttcga gcagcgcgaa ctgctgcacg taacgctcgc gagcatcggc gacggcgtga tcgcgacgga cgtcggcggc cgcatcacgt tcttcaaccg ggtcgccgag gagatgacgg cgctttgcgc cgatgccgtc gtcggtcagc cgatcgagcg ggtgctgatg ttccggcacg cgacgacgca gcgggtgatc gacaatccgg cgcgcgtcgc ggtcgagcgc aggcgcaccg cgagcttgcc tgcccatacg cgcttcgtcg gccgcagcgg cggggagctg ccggtggacg gcaacgccgc accgacgttc gacgcgaacg gcaagctcgt cggcgcggtg ctcatcgtgc gcgacgtgtc ggagcgcgag cgggccgagg agcgattccg gctcgccgtc gaggccgcgc cgaccgcgat gatcatggtg gggcgcgacg gcaggatcgt gctcgtcaat tcgcaggccg agaaattgtt cgggtacgcg cgcggcgaca tgatcgggca cggcatcgac atgctggtac ccgagcgttt tcgtgcgggg cattgggcgc accgcgacgc gttcttcggc aacccggacg cgcggccgat gggcgagggg cgggacctgt tcggtttgcg ccgcgacggc agcgagtttc cggtcgagat cggcctgaat ccgatcacga cctcggaggg cgcgttcgtg ctgtgctcga tcgccgacat caccgcgcgc aagcgcgcgg aactcgagct gcgccgcaga agcgaggagc ttgcgcgctc gaatcaggat ctcgagcagt tcgcctacgt cgcgtctcac gatctgcagg agccgttgcg cgcggtcgcg gggcccctgc aattgctgca gcggcgatac caggggcagc tcgacgcgcg ggccgacgag ttcatcgggc acgcggtgga tggcgcgacg agaatgcagt cgctgatcga cgacctgctc agctattcgc gcgtcgggcg gctcgaggac gcgcgccggc cggtcgactg cgagcacgtg ctggacgagg cgctgcgcaa cctgagcgcg gcgattcgcg aatccggcgc gcaggtcacg cacgatccgc tgccgaccgt gcatggcatc gaggcacagc tcgtgctgct gttccagaat ctgatcggca acgcgatcaa gtttcgcggc aaggaccgcg tcgcgcggat tcatctggaa gcgcacacgc gcgacgacgc atggctgttg cgcgtgcgcg acaacggcat cgggatcgat ccgcagtatt tcgagcgcat cttcctgatt ttccagcggc tgcacacgcg gcgcgaatat ccgggaacgg ggcttgggct cgcgctgtgc aagcggatcg tcgagcacca cggcggccgc atcttcgtcg agtccgagcc ggggcagggt acgacgtttt cgtttacgct gtccaggccg ggtgccgatg aaacgcgttg gccgcgcgat ccgagggagt ga

Details for BumaA.00863.a.B2.GE42199

HARVESTED ON:	2/23/2018
SEQUENCED ON:	3/1/2018
EXPECTED MW:	57kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMR RLLLVEAAAA HDLFEQRELL HVTLASIGDG VIATDVGGRI TFFNRVAEEM TALCADAVVG QPIERVLMFR HATTQRVIDN PARVAVERRR TASLPAHTRF VGRSGGELPV DGNAAPTFDA NGKLVGAVLI VRDVSERERA EERFRLAVEA APTAMIMVGR DGRIVLVNSQ AEKLFGYARG DMIGHGIDML VPERFRAGHW AHRDAFFGNP DARPMGEGRD LFGLRRDGSE FPVEIGLNPI TTSEGAFVLC SIADITARKR AELELRRRSE ELARSNQDLE QFAYVASHDL QEPLRAVAGP LQLLQRRYQG QLDARADEFI GHAVDGATRM QSLIDDLLSY SRVGRLEDAR RPVDCEHVLD EALRNLSAAI RESGAQVTHD PLPTVHGIEA QLVLLFQNLI GNAIKFRGKD RVARIHLEAH TRDDAWLLRV RDNGIGIDPQ YFERIFLIFQ RLHTRREYPG TGLGLALCKR IVEHHGGRIF VESEPGQGTT F

Validated NT Sequence

gaaaacgtcg taccctgccc cggctcggac tcgacgaaga tgcggccgcc gtggtgctcg acgatccgct tgcacagcgc gagcccaagc cccgttcccg gatattcgcg ccgcgtgtgc agccgctgga aaatcaggaa gatgcgctcg aaatactgcg gatcgatccc gatgccgttg tcgcgcacgc gcaacagcca tgcgtcgtcg cgcgtgtgcg cttccagatg aatccgcgcg acgcggtcct tgccgcgaaa cttgatcgcg ttgccgatca gattctggaa cagcagcacg agctgtgcct cgatgccatg cacggtcggc agcggatcgt gcgtgacctg cgcgccggat tcgcgaatcg ccgcgctcag gttgcgcagc gcctcgtcca gcacgtgctc gcagtcgacc ggccggcgcg cgtcctcgag ccgcccgacg cgcgaatagc tgagcaggtc gtcgatcagc gactgcattc tcgtcgcgcc atccaccgcg tgcccgatga actcgtcggc ccgcgcgtcg agctgcccct ggtatcgccg ctgcagcaat tgcaggggcc ccgcgaccgc gcgcaacggc tcctgcagat cgtgagacgc gacgtaggcg aactgctcga gatcctgatt cgagcgcgca agctcctcgc ttctgcggcg cagctcgagt tccgcgcgct tgcgcgcggt gatgtcggcg atcgagcaca gcacgaacgc gccctccgag gtcgtgatcg gattcaggcc gatctcgacc ggaaactcgc tgccgtcgcg gcgcaaaccg aacaggtccc gcccctcgcc catcggccgc gcgtccgggt tgccgaagaa cgcgtcgcgg tgcgcccaat gccccgcacg aaaacgctcg ggtaccagca tgtcgatgcc gtgcccgatc atgtcgccgc gcgcgtaccc gaacaatttc tcggcctgcg aattgacgag cacgatcctg ccgtcgcgcc ccaccatgat catcgcggtc ggcgcggcct cgacggcgag ccggaatcgc tcctcggccc gctcgcgctc cgacacgtcg cgcacgatga gcaccgcgcc gacgagcttg ccgttcgcgt cgaacgtcgg tgcggcgttg ccgtccaccg gcagctcccc gccgctgcgg ccgacgaagc gcgtatgggc aggcaagctc gcggtgcgcc tgcgctcgac cgcgacgcgc gccggattgt cgatcacccg ctgcgtcgtc gcgtgccgga acatcagcac ccgctcgatc ggctgaccga cgacggcatc ggcgcaaagc gccgtcatct cctcggcgac ccggttgaag aacgtgatgc ggccgccgac gtccgtcgcg atcacgccgt cgccgatgct cgcgagcgtt acgtgcagca gttcgcgctg ctcgaacaga tcgtgggccg cggccgcctc gacgagcagc aggcggcgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gcctgctgct cgtcgaggcg gccgcggccc acgatctgtt cgagcagcgc gaactgctgc acgtaacgct cgcgagcatc ggcgacggcg tgatcgcgac ggacgtcggc ggccgcatca cgttcttcaa ccgggtcgcc gaggagatga cggcgctttg cgccgatgcc gtcgtcggtc agccgatcga gcgggtgctg atgttccggc acgcgacgac gcagcgggtg atcgacaatc cggcgcgcgt cgcggtcgag cgcaggcgca ccgcgagctt gcctgcccat acgcgcttcg tcggccgcag cggcggggag ctgccggtgg acggcaacgc cgcaccgacg ttcgacgcga acggcaagct cgtcggcgcg gtgctcatcg tgcgcgacgt gtcggagcgc gagcgggccg aggagcgatt ccggctcgcc gtcgaggccg cgccgaccgc gatgatcatg gtggggcgcg acggcaggat cgtgctcgtc aattcgcagg ccgagaaatt gttcgggtac gcgcgcggcg acatgatcgg gcacggcatc gacatgctgg tacccgagcg ttttcgtgcg gggcattggg cgcaccgcga cgcgttcttc ggcaacccgg acgcgcggcc gatgggcgag gggcgggacc tgttcggttt gcgccgcgac ggcagcgagt ttccggtcga gatcggcctg aatccgatca cgacctcgga gggcgcgttc gtgctgtgct cgatcgccga catcaccgcg cgcaagcgcg cggaactcga gctgcgccgc agaagcgagg agcttgcgcg ctcgaatcag gatctcgagc agttcgccta cgtcgcgtct cacgatctgc aggagccgtt gcgcgcggtc gcggggcccc tgcaattgct gcagcggcga taccaggggc agctcgacgc gcgggccgac gagttcatcg ggcacgcggt ggatggcgcg acgagaatgc agtcgctgat cgacgacctg ctcagctatt cgcgcgtcgg gcggctcgag gacgcgcgcc ggccggtcga ctgcgagcac gtgctggacg aggcgctgcg caacctgagc gcggcgattc gcgaatccgg cgcgcaggtc acgcacgatc cgctgccgac cgtgcatggc atcgaggcac agctcgtgct gctgttccag aatctgatcg gcaacgcgat caagtttcgc ggcaaggacc gcgtcgcgcg gattcatctg gaagcgcaca cgcgcgacga cgcatggctg ttgcgcgtgc gcgacaacgg catcgggatc gatccgcagt atttcgagcg catcttcctg attttccagc ggctgcacac gcggcgcgaa tatccgggaa cggggcttgg gctcgcgctg tgcaagcgga tcgtcgagca ccacggcggc cgcatcttcg tcgagtccga gccggggcag ggtacgacgt tttcgtttac gctgtccagg ccgggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t