BumaA.00624.a

ABC transporter, periplasmic substrate-binding protein (BMAA0855)

CENTER ID: BumaA.00624.a
ORGANISM: Burkholderia mallei ATCC 23344
ASSOCIATED DISEASE: Glanders
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BumaA.00624.a.B2.GE42198 mature protein 21 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243160.12.peg.4384
UniProt: A0A0H2WDP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGAAAQAQA AAVVLYTADG LENLYRDVLP AFEKQEGVKV NIVTAGSGEV VNRANVEKGS PKADVIVTLP PFIQQAGQFG LLQPYRSVNY KNVPAIAKAE DGSWATFVNN YFSFAINPSV VKSQPKTFAD LLHPDYSGKL AYSNPATAGD GMAVIILTSA LMGEDKAFDY LAKLERSVKF HTKGTGYLNV LLSRNEIAVA NGDLQMDLDD AEHGGLSIKP IFVAAKAGEP PTTFQLPYAI GLVKGGPNQD AGRKLIDYLM SADVQAKVPD MFGIPGRTDV PLSGKNGEAV KRAIAGVKLI PVDWDAVMAK KPVWTERWKK EVIGDSGKQT EVVKPK
NT Sequence
ttgcaaggcg cggcagcgca ggcgcaggcg gcagccgtcg tgctgtacac ggcggacggc ctcgagaacc tgtaccgcga cgtgctgccc gcgttcgaga agcaggaggg cgtgaaggtg aacatcgtga cggcgggcag cggcgaagtg gtgaaccgcg cgaacgtcga gaagggctcg ccgaaggccg acgtgatcgt cacgctgccg ccgttcattc agcaggccgg ccagttcggc ctgctgcagc cgtaccgcag cgtcaactac aagaacgtgc cggcgatcgc gaaggcggaa gacggctcat gggcgacgtt cgtcaacaac tacttctcgt tcgcgatcaa cccgtcggtc gtgaagagcc agccgaagac gttcgccgat ctgctgcatc ccgattacag cggcaagctc gcgtattcga acccggcgac ggcgggcgac gggatggccg tcatcatcct gacgagcgcg ctgatgggcg aggacaaggc gttcgactat ctcgcgaagc tcgagcgcag cgtgaagttc cacacgaagg gcacgggcta cctgaacgtg ctgctgtcgc gcaacgagat cgcggtcgcg aacggcgatc tgcagatgga tctggacgac gccgagcacg gcggcctgtc gatcaagccg atcttcgtcg ccgcgaaggc gggcgagccg ccgacgacgt tccagctgcc gtacgcgatc ggcctcgtca agggcggccc gaaccaggac gcgggcagga agctgatcga ctacctgatg tcggccgacg tgcaggcgaa ggtgcccgac atgttcggca ttccgggccg caccgacgtg ccgctttcgg gcaagaacgg cgaggcggtg aagcgcgcga tcgccggcgt gaagctgatt ccggtcgact gggacgcggt gatggcgaag aagcccgtgt ggaccgagcg ctggaagaag gaagtgatcg gcgattcggg caagcagacc gaagtcgtca agccgaaatg a
Details for BumaA.00624.a.B2.GE42198
HARVESTED ON: 2/23/2018
SEQUENCED ON: 3/1/2018
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML ENLYRDVLPA FEKQEGVKVN IVTAGSGEVV NRANVEKGSP KADVIVTLPP FIQQAGQFGL LQPYRSVNYK NVPAIAKAED GSWATFVNNY FSFAINPSVV KSQPKTFADL LHPDYSGKLA YSNPATAGDG MAVIILTSAL MGEDKAFDYL AKLERSVKFH TKGTGYLNVL LSRNEIAVAN GDLQMDLDDA EHGGLSIKPI FVAAKAGEPP TTFQLPYAIG LVKGGPNQDA GRKLIDYLMS ADVQAKVPDM FGIPGRTDVP LSGKNGEAVK RAIAGVKLIP VDWDAVMAKK PVWTERWKKE VIGDSGKQXX VVKPK
Validated NT Sequence
ttactcactt atttcggctt gacgacttnn nnctgcttgc ccgaatcgcc gatcacttcc ttcttccagc gctcggtcca cacgggcttc ttcgccatca ccgcgtccca gtcgaccgga atcagcttca cgccggcgat cgcgcgcttc accgcctcgc cgttcttgcc cgaaagcggc acgtcggtgc ggcccggaat gccgaacatg tcgggcacct tcgcctgcac gtcggccgac atcaggtagt cgatcagctt cctgcccgcg tcctggttcg ggccgccctt gacgaggccg atcgcgtacg gcagctggaa cgtcgtcggc ggctcgcccg ccttcgcggc gacgaagatc ggcttgatcg acaggccgcc gtgctcggcg tcgtccagat ccatctgcag atcgccgttc gcgaccgcga tctcgttgcg cgacagcagc acgttcaggt agcccgtgcc cttcgtgtgg aacttcacgc tgcgctcgag cttcgcgaga tagtcgaacg ccttgtcctc gcccatcagc gcgctcgtca ggatgatgac ggccatcccg tcgcccgccg tcgccgggtt cgaatacgcg agcttgccgc tgtaatcggg atgcagcaga tcggcgaacg tcttcggctg gctcttcacg accgacgggt tgatcgcgaa cgagaagtag ttgttgacga acgtcgccca tgagccgtct tccgccttcg cgatcgccgg cacgttcttg tagttgacgc tgcggtacgg ctgcagcagg ccgaactggc cggcctgctg aatgaacggc ggcagcgtga cgatcacgtc ggccttcggc gagcccttct cgacgttcgc gcggttcacc acttcgccgc tgcccgccgt cacgatgttc accttcacgc cctcctgctt ctcgaacgcg ggcagcacgt cgcggtacag gttctcgagc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHLE NLYRDVLPAF EKQEGVKVNI VTAGSGEVVN RANVEKGSPK ADVIVTLPPF IQQAGQFGLL QPYRSVNYKN VPAIAKAEDG SWATFVNNYF SFAINPSVVK SQPKTFADLL HPDYSGKLAY SNPATAGDGM AVIILTSALM GEDKAFDYLA KLERSVKFHT KGTGYLNVLL SRNEIAVANG DLQMDLDDAE HGGLSIKPIF VAAKAGEPPT TFQLPYAIGL VKGGPNQDAG RKLIDYLMSA DVQAKVPDMF GIPGRTDVPL SGKNGEAVKR AIAGVKLIPV DWDAVMAKKP VWTERWKKEV IGDSGKQTEV VKPK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctcgaga acctgtaccg cgacgtgctg cccgcgttcg agaagcagga gggcgtgaag gtgaacatcg tgacggcggg cagcggcgaa gtggtgaacc gcgcgaacgt cgagaagggc tcgccgaagg ccgacgtgat cgtcacgctg ccgccgttca ttcagcaggc cggccagttc ggcctgctgc agccgtaccg cagcgtcaac tacaagaacg tgccggcgat cgcgaaggcg gaagacggct catgggcgac gttcgtcaac aactacttct cgttcgcgat caacccgtcg gtcgtgaaga gccagccgaa gacgttcgcc gatctgctgc atcccgatta cagcggcaag ctcgcgtatt cgaacccggc gacggcgggc gacgggatgg ccgtcatcat cctgacgagc gcgctgatgg gcgaggacaa ggcgttcgac tatctcgcga agctcgagcg cagcgtgaag ttccacacga agggcacggg ctacctgaac gtgctgctgt cgcgcaacga gatcgcggtc gcgaacggcg atctgcagat ggatctggac gacgccgagc acggcggcct gtcgatcaag ccgatcttcg tcgccgcgaa ggcgggcgag ccgccgacga cgttccagct gccgtacgcg atcggcctcg tcaagggcgg cccgaaccag gacgcgggca ggaagctgat cgactacctg atgtcggccg acgtgcaggc gaaggtgccc gacatgttcg gcattccggg ccgcaccgac gtgccgcttt cgggcaagaa cggcgaggcg gtgaagcgcg cgatcgccgg cgtgaagctg attccggtcg actgggacgc ggtgatggcg aagaagcccg tgtggaccga gcgctggaag aaggaagtga tcggcgattc gggcaagcag accgaagtcg tcaagccgaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat