BuceA.19268.a

D-lactate dehydrogenase

CENTER ID: BuceA.19268.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.19268.a.B1.GE40337 Full length(BuceA.19268.a) 1 582
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.19268.a.B1.PS38419 Full length(BuceA.19268.a) 1 582

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2601
UniProt: B4E754

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTVSGESSS PLAPPTGRHE AFIRSLTEIV GQQDVLTNER DTRRYRTGFR FGEGRTLAVV RPGTLLQQWK VLQACVAANV IVICQASNTG LTGGSTPDGD DYDRDIVIVS TARIRKVHVI KSGKQVICLP GATLDQLEQT LKPLGREPHS VIGSSCIGAS VIGGVCNNSG GALVQRGPAY TEMSVFAQVG ADGRLKLVNH LDVRLGAVPE EILARLDNGD FSERDIVDGD ASGSDHHYAE HVRDVDADTP ARYNADPLRL HEASGSAGKV MVFAVRLDTF PIEAGAKVFY IGTNDIDELT DIRRHALTQF KRLPIAGEYL HRDAFDIAQK YGKDTFLMIR HFGTKRMPML FGFKSKCDAW FDRLGFLPRH LSDRVMQAVS NLFPNHLPPR IRDYGKRYEH HLMLKVSADT ADEARSFLTS FFAKRTGAFF ECTPAEGDKA FLHRFAAAGA AIRYRNVHTR DVEDIVALDI ALRRNDRDWF ETLPPDIDAP IAIKLYYGHF LCHVFHQDYI VKKGNDCLAL EHAMWKLLDA RRAEYPAEHN VGHLYHAKPA LKAFYEGLDP CNCFNPGIGQ TSKFAKYREA HD
NT Sequence
GTGTCAACCG TATCAGGCGA ATCGTCTTCG CCGCTTGCGC CGCCCACCGG GCGGCATGAG GCGTTCATCC GTTCGCTGAC AGAAATCGTC GGCCAGCAGG ACGTGCTGAC GAACGAGCGG GACACGCGGC GCTACCGGAC CGGCTTCCGG TTCGGCGAAG GCCGGACGCT CGCGGTGGTG CGGCCCGGCA CGCTGCTCCA GCAATGGAAG GTGCTGCAGG CCTGCGTGGC CGCGAACGTG ATCGTGATCT GCCAGGCGTC GAACACGGGC CTCACCGGCG GCTCGACGCC CGACGGCGAC GACTACGACC GCGACATCGT GATCGTCAGC ACCGCGCGGA TCCGCAAGGT GCACGTCATC AAGAGCGGCA AGCAGGTGAT CTGCCTGCCG GGCGCGACGC TCGATCAGCT CGAGCAGACG CTGAAACCGC TCGGGCGCGA GCCGCACTCG GTGATCGGCT CGTCGTGCAT CGGCGCATCG GTGATCGGCG GCGTCTGCAA CAACTCCGGC GGCGCGCTGG TGCAGCGCGG GCCGGCTTAT ACGGAGATGT CGGTGTTCGC CCAGGTCGGT GCGGACGGGC GCCTGAAGCT CGTGAACCAT CTCGACGTGC GCCTGGGCGC GGTGCCGGAG GAAATCCTCG CGCGCCTCGA CAACGGCGAC TTCTCGGAGC GCGACATCGT CGACGGCGAT GCGTCCGGTT CCGATCACCA CTACGCCGAG CACGTGCGCG ACGTCGATGC CGACACGCCC GCGCGCTACA ACGCCGATCC GCTGCGGTTG CACGAAGCAT CGGGCTCGGC CGGCAAGGTG ATGGTGTTCG CGGTGCGCCT CGACACGTTT CCGATCGAAG CCGGTGCGAA GGTGTTCTAC ATCGGCACCA ACGACATCGA CGAGCTGACC GACATCCGCC GCCACGCGCT CACGCAGTTC AAGCGCCTGC CGATCGCGGG TGAATACCTG CACCGCGACG CGTTCGACAT CGCGCAGAAG TACGGCAAGG ACACGTTCCT GATGATCCGC CACTTCGGCA CGAAGCGCAT GCCGATGCTG TTCGGCTTCA AGAGCAAATG CGACGCGTGG TTCGACCGGC TCGGCTTCCT GCCGCGGCAT CTGAGCGATC GCGTGATGCA GGCCGTCAGC AACCTGTTTC CGAATCACCT GCCGCCGCGC ATTCGCGACT ACGGCAAGCG CTACGAACAT CATCTGATGC TGAAGGTATC GGCCGATACC GCGGACGAAG CGCGCAGCTT CCTGACCTCG TTCTTCGCGA AGCGCACGGG CGCGTTCTTC GAATGCACGC CGGCGGAAGG CGACAAGGCG TTCCTGCACC GGTTCGCGGC GGCCGGCGCG GCGATCCGCT ACCGCAACGT GCATACGCGC GACGTGGAGG ACATCGTCGC GCTCGACATC GCGCTTCGCC GCAACGATCG CGACTGGTTC GAGACGCTGC CGCCCGACAT CGACGCGCCG ATCGCGATCA AGCTGTATTA CGGCCACTTC CTGTGCCACG TGTTCCACCA GGACTACATC GTCAAGAAGG GCAACGACTG CCTCGCGCTC GAACACGCGA TGTGGAAGCT GCTCGACGCG CGCCGCGCCG AATATCCGGC CGAGCACAAC GTCGGGCACC TGTACCACGC GAAGCCGGCG CTCAAGGCGT TCTACGAAGG GCTCGACCCG TGCAACTGCT TCAACCCGGG GATCGGCCAG ACGTCGAAGT TCGCGAAGTA CCGCGAAGCG CACGAC
Details for BuceA.19268.a.B1.GE40337
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 66kDa
OBSERVED MW: 66kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 87
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV STVSGESSSP LAPPTGRHEA FIRSLTEIVG QQDVLTNERD TRRYRTGFRF GEGRTLAVVR PGTLLQQWKV LQACVAANVI VICQASNTGL TGGSTPDGDD YDRDIVIVST ARIRKVHVIK SGKQVICLPG ATLDQLEQTL KPLGREPHSV IGSSCIGASV IGGVCNNSGG ALVQRGPAYT EMSVFAQVGA DGRLKLVNHL DVRLGAXXXE ILARLDNGDF XXRDIXXGXX XXXXXXYXXH XXXVXXXTXX RXXXXXLRLX XXXXXXXXXX XXXXXXXTXX XXXXXXXXXX XXXXXXELTD XXRHXXTQFK RLPIAXXYLH RDAFDIAQKY GKDTFLMIRH FGTKRMPMLF GFKSKCDAWF DRLGFLPRHL SDRVMQAVSN LFPNHLPPRI RDYGKRYEHH LMLKVSADTA DEARSFLTSF FAKRTGAFFE CTPAEGDKAF LHRFAAAGAA IRYRNVHTRD VEDIVALDIA LRRNDRDWFE TLPPDIDAPI AIKLYYGHFL CHVFHQDYIV KKGNDCLALE HAMWKLLDAR RAEYPAEHNV GHLYHAKPAL KAFYEGLDPC NCFNPGIGQT SKFA
Validated NT Sequence
tcgcgaactt cgacgtctgg ccgatccccg ggttgaagca gttgcacggg tcgagccctt cgtagaacgc cttgagcgcc ggcttcgcgt ggtacaggtg cccgacgttg tgctcggccg gatattcggc gcggcgcgcg tcgagcagct tccacatcgc gtgttcgagc gcgaggcagt cgttgccctt cttgacgatg tagtcctggt ggaacacgtg gcacaggaag tggccgtaat acagcttgat cgcgatcggc gcgtcgatgt cgggcggcag cgtctcgaac cagtcgcgat cgttgcggcg aagcgcgatg tcgagcgcga cgatgtcctc cacgtcgcgc gtatgcacgt tgcggtagcg gatcgccgcg ccggccgccg cgaaccggtg caggaacgcc ttgtcgcctt ccgccggcgt gcattcgaag aacgcgcccg tgcgcttcgc gaagaacgag gtcaggaagc tgcgcgcttc gtccgcggta tcggccgata ccttcagcat cagatgatgt tcgtagcgct tgccgtagtc gcgaatgcgc ggcggcaggt gattcggaaa caggttgctg acggcctgca tcacgcgatc gctcagatgc cgcggcagga agccgagccg gtcgaaccac gcgtcgcatt tgctcttgaa gccgaacagc atcggcatgc gcttcgtgcc gaagtggcgg atcatcagga acgtgtcctt gccgtacttc tgcgcgatgt cgaacgcgtc gcggtgcagg tattnnnnng cgatcggcag gcgcttgaac tgcgtgnnnn cgtggcggnn natgtcggtc agctcnnnnn tgtnnntgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnnnggnnn anncnnnnnn nnnnnncaac cgcagcgnnn nnnnnnnnnn ncgcnnnnnn gtnnnnnnan nnacgtnnnn nnngtgnnnn ncgtannnnn nntcggnnnn nnncncnncg ccgnnnnnga tgtcgcgcnn nnngaagtcg ccgttgtcga ggcgcgcgag gatttcctnn nnnnccgcgc ccaggcgcac gtcgagatgg ttcacgagct tcaggcgccc gtccgcaccg acctgggcga acaccgacat ctccgtataa gccggcccgc gctgcaccag cgcgccgccg gagttgttgc agacgccgcc gatcaccgat gcgccgatgc acgacgagcc gatcaccgag tgcggctcgc gcccgagcgg tttcagcgtc tgctcgagct gatcgagcgt cgcgcccggc aggcagatca cctgcttgcc gctcttgatg acgtgcacct tgcggatccg cgcggtgctg acgatcacga tgtcgcggtc gtagtcgtcg ccgtcgggcg tcgagccgcc ggtgaggccc gtgttcgacg cctggcagat cacgatcacg ttcgcggcca cgcaggcctg cagcaccttc cattgctgga gcagcgtgcc gggccgcacc accgcgagcg tccggccttc gccgaaccgg aagccggtcc ggtagcgccg cgtgtcccgc tcgttcgtca gcacgtcctg ctggccgacg atttctgtca gcgaacggat gaacgcctca tgccgcccgg tgggcggcgc aagcggcgaa gacgattcgc ctgatacggt tgacaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS TVSGESSSPL APPTGRHEAF IRSLTEIVGQ QDVLTNERDT RRYRTGFRFG EGRTLAVVRP GTLLQQWKVL QACVAANVIV ICQASNTGLT GGSTPDGDDY DRDIVIVSTA RIRKVHVIKS GKQVICLPGA TLDQLEQTLK PLGREPHSVI GSSCIGASVI GGVCNNSGGA LVQRGPAYTE MSVFAQVGAD GRLKLVNHLD VRLGAVPEEI LARLDNGDFS ERDIVDGDAS GSDHHYAEHV RDVDADTPAR YNADPLRLHE ASGSAGKVMV FAVRLDTFPI EAGAKVFYIG TNDIDELTDI RRHALTQFKR LPIAGEYLHR DAFDIAQKYG KDTFLMIRHF GTKRMPMLFG FKSKCDAWFD RLGFLPRHLS DRVMQAVSNL FPNHLPPRIR DYGKRYEHHL MLKVSADTAD EARSFLTSFF AKRTGAFFEC TPAEGDKAFL HRFAAAGAAI RYRNVHTRDV EDIVALDIAL RRNDRDWFET LPPDIDAPIA IKLYYGHFLC HVFHQDYIVK KGNDCLALEH AMWKLLDARR AEYPAEHNVG HLYHAKPALK AFYEGLDPCN CFNPGIGQTS KFAKYREAHD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcaa ccgtatcagg cgaatcgtct tcgccgcttg cgccgcccac cgggcggcat gaggcgttca tccgttcgct gacagaaatc gtcggccagc aggacgtgct gacgaacgag cgggacacgc ggcgctaccg gaccggcttc cggttcggcg aaggccggac gctcgcggtg gtgcggcccg gcacgctgct ccagcaatgg aaggtgctgc aggcctgcgt ggccgcgaac gtgatcgtga tctgccaggc gtcgaacacg ggcctcaccg gcggctcgac gcccgacggc gacgactacg accgcgacat cgtgatcgtc agcaccgcgc ggatccgcaa ggtgcacgtc atcaagagcg gcaagcaggt gatctgcctg ccgggcgcga cgctcgatca gctcgagcag acgctgaaac cgctcgggcg cgagccgcac tcggtgatcg gctcgtcgtg catcggcgca tcggtgatcg gcggcgtctg caacaactcc ggcggcgcgc tggtgcagcg cgggccggct tatacggaga tgtcggtgtt cgcccaggtc ggtgcggacg ggcgcctgaa gctcgtgaac catctcgacg tgcgcctggg cgcggtgccg gaggaaatcc tcgcgcgcct cgacaacggc gacttctcgg agcgcgacat cgtcgacggc gatgcgtccg gttccgatca ccactacgcc gagcacgtgc gcgacgtcga tgccgacacg cccgcgcgct acaacgccga tccgctgcgg ttgcacgaag catcgggctc ggccggcaag gtgatggtgt tcgcggtgcg cctcgacacg tttccgatcg aagccggtgc gaaggtgttc tacatcggca ccaacgacat cgacgagctg accgacatcc gccgccacgc gctcacgcag ttcaagcgcc tgccgatcgc gggtgaatac ctgcaccgcg acgcgttcga catcgcgcag aagtacggca aggacacgtt cctgatgatc cgccacttcg gcacgaagcg catgccgatg ctgttcggct tcaagagcaa atgcgacgcg tggttcgacc ggctcggctt cctgccgcgg catctgagcg atcgcgtgat gcaggccgtc agcaacctgt ttccgaatca cctgccgccg cgcattcgcg actacggcaa gcgctacgaa catcatctga tgctgaaggt atcggccgat accgcggacg aagcgcgcag cttcctgacc tcgttcttcg cgaagcgcac gggcgcgttc ttcgaatgca cgccggcgga aggcgacaag gcgttcctgc accggttcgc ggcggccggc gcggcgatcc gctaccgcaa cgtgcatacg cgcgacgtgg aggacatcgt cgcgctcgac atcgcgcttc gccgcaacga tcgcgactgg ttcgagacgc tgccgcccga catcgacgcg ccgatcgcga tcaagctgta ttacggccac ttcctgtgcc acgtgttcca ccaggactac atcgtcaaga agggcaacga ctgcctcgcg ctcgaacacg cgatgtggaa gctgctcgac gcgcgccgcg ccgaatatcc ggccgagcac aacgtcgggc acctgtacca cgcgaagccg gcgctcaagg cgttctacga agggctcgac ccgtgcaact gcttcaaccc ggggatcggc cagacgtcga agttcgcgaa gtaccgcgaa gcgcacgact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuceA.19268.a.B1.PS38419
PURIFICATION DATe: 3/6/2018
CONCENTRATION: 35.5mg/ml
OBSERVED MW: 78kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 87
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV STVSGESSSP LAPPTGRHEA FIRSLTEIVG QQDVLTNERD TRRYRTGFRF GEGRTLAVVR PGTLLQQWKV LQACVAANVI VICQASNTGL TGGSTPDGDD YDRDIVIVST ARIRKVHVIK SGKQVICLPG ATLDQLEQTL KPLGREPHSV IGSSCIGASV IGGVCNNSGG ALVQRGPAYT EMSVFAQVGA DGRLKLVNHL DVRLGAXXXE ILARLDNGDF XXRDIXXGXX XXXXXXYXXH XXXVXXXTXX RXXXXXLRLX XXXXXXXXXX XXXXXXXTXX XXXXXXXXXX XXXXXXELTD XXRHXXTQFK RLPIAXXYLH RDAFDIAQKY GKDTFLMIRH FGTKRMPMLF GFKSKCDAWF DRLGFLPRHL SDRVMQAVSN LFPNHLPPRI RDYGKRYEHH LMLKVSADTA DEARSFLTSF FAKRTGAFFE CTPAEGDKAF LHRFAAAGAA IRYRNVHTRD VEDIVALDIA LRRNDRDWFE TLPPDIDAPI AIKLYYGHFL CHVFHQDYIV KKGNDCLALE HAMWKLLDAR RAEYPAEHNV GHLYHAKPAL KAFYEGLDPC NCFNPGIGQT SKFA
Validated NT Sequence
tcgcgaactt cgacgtctgg ccgatccccg ggttgaagca gttgcacggg tcgagccctt cgtagaacgc cttgagcgcc ggcttcgcgt ggtacaggtg cccgacgttg tgctcggccg gatattcggc gcggcgcgcg tcgagcagct tccacatcgc gtgttcgagc gcgaggcagt cgttgccctt cttgacgatg tagtcctggt ggaacacgtg gcacaggaag tggccgtaat acagcttgat cgcgatcggc gcgtcgatgt cgggcggcag cgtctcgaac cagtcgcgat cgttgcggcg aagcgcgatg tcgagcgcga cgatgtcctc cacgtcgcgc gtatgcacgt tgcggtagcg gatcgccgcg ccggccgccg cgaaccggtg caggaacgcc ttgtcgcctt ccgccggcgt gcattcgaag aacgcgcccg tgcgcttcgc gaagaacgag gtcaggaagc tgcgcgcttc gtccgcggta tcggccgata ccttcagcat cagatgatgt tcgtagcgct tgccgtagtc gcgaatgcgc ggcggcaggt gattcggaaa caggttgctg acggcctgca tcacgcgatc gctcagatgc cgcggcagga agccgagccg gtcgaaccac gcgtcgcatt tgctcttgaa gccgaacagc atcggcatgc gcttcgtgcc gaagtggcgg atcatcagga acgtgtcctt gccgtacttc tgcgcgatgt cgaacgcgtc gcggtgcagg tattnnnnng cgatcggcag gcgcttgaac tgcgtgnnnn cgtggcggnn natgtcggtc agctcnnnnn tgtnnntgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gtnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnnnggnnn anncnnnnnn nnnnnncaac cgcagcgnnn nnnnnnnnnn ncgcnnnnnn gtnnnnnnan nnacgtnnnn nnngtgnnnn ncgtannnnn nntcggnnnn nnncncnncg ccgnnnnnga tgtcgcgcnn nnngaagtcg ccgttgtcga ggcgcgcgag gatttcctnn nnnnccgcgc ccaggcgcac gtcgagatgg ttcacgagct tcaggcgccc gtccgcaccg acctgggcga acaccgacat ctccgtataa gccggcccgc gctgcaccag cgcgccgccg gagttgttgc agacgccgcc gatcaccgat gcgccgatgc acgacgagcc gatcaccgag tgcggctcgc gcccgagcgg tttcagcgtc tgctcgagct gatcgagcgt cgcgcccggc aggcagatca cctgcttgcc gctcttgatg acgtgcacct tgcggatccg cgcggtgctg acgatcacga tgtcgcggtc gtagtcgtcg ccgtcgggcg tcgagccgcc ggtgaggccc gtgttcgacg cctggcagat cacgatcacg ttcgcggcca cgcaggcctg cagcaccttc cattgctgga gcagcgtgcc gggccgcacc accgcgagcg tccggccttc gccgaaccgg aagccggtcc ggtagcgccg cgtgtcccgc tcgttcgtca gcacgtcctg ctggccgacg atttctgtca gcgaacggat gaacgcctca tgccgcccgg tgggcggcgc aagcggcgaa gacgattcgc ctgatacggt tgacaccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS TVSGESSSPL APPTGRHEAF IRSLTEIVGQ QDVLTNERDT RRYRTGFRFG EGRTLAVVRP GTLLQQWKVL QACVAANVIV ICQASNTGLT GGSTPDGDDY DRDIVIVSTA RIRKVHVIKS GKQVICLPGA TLDQLEQTLK PLGREPHSVI GSSCIGASVI GGVCNNSGGA LVQRGPAYTE MSVFAQVGAD GRLKLVNHLD VRLGAVPEEI LARLDNGDFS ERDIVDGDAS GSDHHYAEHV RDVDADTPAR YNADPLRLHE ASGSAGKVMV FAVRLDTFPI EAGAKVFYIG TNDIDELTDI RRHALTQFKR LPIAGEYLHR DAFDIAQKYG KDTFLMIRHF GTKRMPMLFG FKSKCDAWFD RLGFLPRHLS DRVMQAVSNL FPNHLPPRIR DYGKRYEHHL MLKVSADTAD EARSFLTSFF AKRTGAFFEC TPAEGDKAFL HRFAAAGAAI RYRNVHTRDV EDIVALDIAL RRNDRDWFET LPPDIDAPIA IKLYYGHFLC HVFHQDYIVK KGNDCLALEH AMWKLLDARR AEYPAEHNVG HLYHAKPALK AFYEGLDPCN CFNPGIGQTS KFAKYREAHD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTCAA CCGTATCAGG CGAATCGTCT TCGCCGCTTG CGCCGCCCAC CGGGCGGCAT GAGGCGTTCA TCCGTTCGCT GACAGAAATC GTCGGCCAGC AGGACGTGCT GACGAACGAG CGGGACACGC GGCGCTACCG GACCGGCTTC CGGTTCGGCG AAGGCCGGAC GCTCGCGGTG GTGCGGCCCG GCACGCTGCT CCAGCAATGG AAGGTGCTGC AGGCCTGCGT GGCCGCGAAC GTGATCGTGA TCTGCCAGGC GTCGAACACG GGCCTCACCG GCGGCTCGAC GCCCGACGGC GACGACTACG ACCGCGACAT CGTGATCGTC AGCACCGCGC GGATCCGCAA GGTGCACGTC ATCAAGAGCG GCAAGCAGGT GATCTGCCTG CCGGGCGCGA CGCTCGATCA GCTCGAGCAG ACGCTGAAAC CGCTCGGGCG CGAGCCGCAC TCGGTGATCG GCTCGTCGTG CATCGGCGCA TCGGTGATCG GCGGCGTCTG CAACAACTCC GGCGGCGCGC TGGTGCAGCG CGGGCCGGCT TATACGGAGA TGTCGGTGTT CGCCCAGGTC GGTGCGGACG GGCGCCTGAA GCTCGTGAAC CATCTCGACG TGCGCCTGGG CGCGGTGCCG GAGGAAATCC TCGCGCGCCT CGACAACGGC GACTTCTCGG AGCGCGACAT CGTCGACGGC GATGCGTCCG GTTCCGATCA CCACTACGCC GAGCACGTGC GCGACGTCGA TGCCGACACG CCCGCGCGCT ACAACGCCGA TCCGCTGCGG TTGCACGAAG CATCGGGCTC GGCCGGCAAG GTGATGGTGT TCGCGGTGCG CCTCGACACG TTTCCGATCG AAGCCGGTGC GAAGGTGTTC TACATCGGCA CCAACGACAT CGACGAGCTG ACCGACATCC GCCGCCACGC GCTCACGCAG TTCAAGCGCC TGCCGATCGC GGGTGAATAC CTGCACCGCG ACGCGTTCGA CATCGCGCAG AAGTACGGCA AGGACACGTT CCTGATGATC CGCCACTTCG GCACGAAGCG CATGCCGATG CTGTTCGGCT TCAAGAGCAA ATGCGACGCG TGGTTCGACC GGCTCGGCTT CCTGCCGCGG CATCTGAGCG ATCGCGTGAT GCAGGCCGTC AGCAACCTGT TTCCGAATCA CCTGCCGCCG CGCATTCGCG ACTACGGCAA GCGCTACGAA CATCATCTGA TGCTGAAGGT ATCGGCCGAT ACCGCGGACG AAGCGCGCAG CTTCCTGACC TCGTTCTTCG CGAAGCGCAC GGGCGCGTTC TTCGAATGCA CGCCGGCGGA AGGCGACAAG GCGTTCCTGC ACCGGTTCGC GGCGGCCGGC GCGGCGATCC GCTACCGCAA CGTGCATACG CGCGACGTGG AGGACATCGT CGCGCTCGAC ATCGCGCTTC GCCGCAACGA TCGCGACTGG TTCGAGACGC TGCCGCCCGA CATCGACGCG CCGATCGCGA TCAAGCTGTA TTACGGCCAC TTCCTGTGCC ACGTGTTCCA CCAGGACTAC ATCGTCAAGA AGGGCAACGA CTGCCTCGCG CTCGAACACG CGATGTGGAA GCTGCTCGAC GCGCGCCGCG CCGAATATCC GGCCGAGCAC AACGTCGGGC ACCTGTACCA CGCGAAGCCG GCGCTCAAGG CGTTCTACGA AGGGCTCGAC CCGTGCAACT GCTTCAACCC GGGGATCGGC CAGACGTCGA AGTTCGCGAA GTACCGCGAA GCGCACGACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT