BuceA.18597.a

Putative purine nucleoside permease

CENTER ID: BuceA.18597.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18597.a.B1.GE37125 full length 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6367
RefSeq: YP_002235159.1
UniProt: B4EJQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTRSILSAA AFSLAACATA PSIAQDNQGN DAGNAAFAET GSHGRPVKVM IITMFGPEGQ AWLDRLGPWR DITVPGLSPD YPAVHCNKQD VCVVTTGMGY ANASATIMAL TFSPRFDLRR TYFLISGIAG VDPAQGTVGS AAWSKYLVDF SLQWELDARE IPAGWNTGFL GINTKSPNDK PPLDYRTEVF QLNPQLTDAA YALSRNVVLA DSAQAQAARA KFTYAPANRP PTVIQCDTSS GNTWFSGTLI GERARQWTKI LTDGKGTYCM TAQEDNATYE ALKRAASVKR VDLSRVAVLR TGSDFDRPYA GQTSTDNLLN YADQGGFAPA TENLYRAGNP LVQDIVTHWG EWRDGVPRR
NT Sequence
atgctgactc gctccatcct ttccgccgcc gcattctcgc ttgccgcctg tgcgaccgcg ccgtcgatcg cgcaggacaa ccagggcaac gatgcaggca atgcagcatt cgccgagacc ggctcgcatg gccgcccggt caaggtcatg atcatcacga tgttcggccc ggaaggccag gcatggctcg accggctcgg cccgtggcgc gacatcaccg tgccgggcct gtcgcccgac tatccggctg tccactgcaa caagcaggac gtgtgcgtgg tcacgaccgg gatgggctat gcgaacgcgt cggcgacgat catggcgctc acgttctcgc cacgcttcga tctgcgccgc acctatttcc tgatctcggg catcgcgggc gtcgaccccg cacagggcac ggtcggatcg gccgcgtggt cgaaatacct cgtcgatttc agcctgcagt gggagctcga tgcgcgcgag attccggccg ggtggaacac cggctttctc ggcatcaaca cgaagagccc gaacgacaag ccgccgctcg actatcgcac cgaagtgttc cagctcaacc cgcagctgac cgacgccgcg tacgcgctgt cgcgcaacgt cgtgctcgcc gatagcgccc aggcgcaggc tgcgcgcgcg aagttcacgt atgcgccggc gaaccggccg ccgacggtga tccagtgcga tacgtcgtcg ggcaacacgt ggttctcggg cacgctgatc ggcgagcgcg cgcgtcagtg gaccaagatc ctgacggacg gcaagggcac ctactgcatg accgcgcagg aggacaacgc gacgtatgaa gcgctcaagc gcgcggcgag cgtgaagcgg gtcgacctgt cgcgtgtggc cgtgctgcgc accgggtccg atttcgatcg gccgtacgcg gggcagacga gcaccgacaa cctgctgaac tacgcggacc agggcggctt cgccccggcg accgaaaacc tgtaccgcgc gggcaatccg ctggtgcagg acatcgtcac gcactggggc gaatggcgcg acggcgtgcc gcgtcgttga
Details for BuceA.18597.a.B1.GE37125
HARVESTED ON: 7/17/2013
SEQUENCED ON: 7/20/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Validated AA Sequence
MIITMFGPEG QAWLDRLGPW RDITVPGLSP DYPAVHCNKQ DVCVVTTGMG YANASATIMA LTFSPRFDLR RTYFLISGIA GVDPAQGTVG SAAWSKYLVD FSLQWELDAR EIPAGWNTGF LGINTKSPND KPPLDYRTEV FQLNPQLTDA AYALSRNVVL ADSAQAQAAR AKFTYAPANR PPTVIQCDTS SGNTWFSGTL IGERARQWTK ILTDGKGTYC MTAQEDNATY EALKRAASVK RVDLXRVAVX RTGSDFDRPY AGQTSTDNLL NYADQGGFAP ATENLYRAGN PLVQDIVTHW
Validated NT Sequence
gccccagtgc gtgacgatgt cctgcaccag cggattgccc gcgcggtaca ggttttcggt cgccggggcg aagccgccct ggtccgcgta gttcagcagg ttgtcggtgc tcgtctgccc cgcgtacggc cgatcgaaat cggacccggt gcgcancacg gccacncgcn acaggtcgac ccgcttcacg ctcgccgcgc gcttgagcgc ttcatacgtc gcgttgtcct cctgcgcggt catgcagtan gtgcccttgc cgtccgtcag gatcttggtc cactgacgcg cgcgctcgcc gatcagcgtg cccgagaacc acgtgttgcc cgacgacgta tcgcactgga tcaccgtcgg cggccggttc gccggcgcat acgtgaactt cgcgcgcgca gcctgcgcct gggcgctatc ggcgagcacg acgttgcgcg acagcgcgta cgcggcgtcg gtcagctgcg ggttgagctg gaacacttcg gtgcgatagt cgagcggcgg cttgtcgttc gggctcttcg tgttgatgcc gagaaagccg gtgttccacc cggccggaat ctcgcgcgca tcgagctccc actgcaggct gaaatcgacg aggtatttcg accacgcggc cgatccgacc gtgccctgtg cggggtcgac gcccgcgatg cccgagatca ggaaataggt gcggcgcaga tcgaagcgtg gcgagaacgt gagcgccatg atcgtcgccg acgcgttcgc atagcccatc ccggtcgtga ccacgcacac gtcctgcttg ttgcagtgga cagccggata gtcgggcgac aggcccggca cggtgatgtc gcgccacggg ccgagccggt cgagccatgc ctggccttcc gggccgaaca tcgtgatgat catgaccttg accgggcggc catgcgagcc ggtctcggcg aatgctgcat tgcctgcatc gttgccctgg ttgtcctgcg cgatcgacgg cgcggtcgca caggcggcaa gcgagaatgc ggcggcggaa agaatggagc gagtc
Expected Protein Sequence
MAHHHHHHML TRSILSAAAF SLAACATAPS IAQDNQGNDA GNAAFAETGS HGRPVKVMII TMFGPEGQAW LDRLGPWRDI TVPGLSPDYP AVHCNKQDVC VVTTGMGYAN ASATIMALTF SPRFDLRRTY FLISGIAGVD PAQGTVGSAA WSKYLVDFSL QWELDAREIP AGWNTGFLGI NTKSPNDKPP LDYRTEVFQL NPQLTDAAYA LSRNVVLADS AQAQAARAKF TYAPANRPPT VIQCDTSSGN TWFSGTLIGE RARQWTKILT DGKGTYCMTA QEDNATYEAL KRAASVKRVD LSRVAVLRTG SDFDRPYAGQ TSTDNLLNYA DQGGFAPATE NLYRAGNPLV QDIVTHWGEW RDGVPRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctga ctcgctccat cctttccgcc gccgcattct cgcttgccgc ctgtgcgacc gcgccgtcga tcgcgcagga caaccagggc aacgatgcag gcaatgcagc attcgccgag accggctcgc atggccgccc ggtcaaggtc atgatcatca cgatgttcgg cccggaaggc caggcatggc tcgaccggct cggcccgtgg cgcgacatca ccgtgccggg cctgtcgccc gactatccgg ctgtccactg caacaagcag gacgtgtgcg tggtcacgac cgggatgggc tatgcgaacg cgtcggcgac gatcatggcg ctcacgttct cgccacgctt cgatctgcgc cgcacctatt tcctgatctc gggcatcgcg ggcgtcgacc ccgcacaggg cacggtcgga tcggccgcgt ggtcgaaata cctcgtcgat ttcagcctgc agtgggagct cgatgcgcgc gagattccgg ccgggtggaa caccggcttt ctcggcatca acacgaagag cccgaacgac aagccgccgc tcgactatcg caccgaagtg ttccagctca acccgcagct gaccgacgcc gcgtacgcgc tgtcgcgcaa cgtcgtgctc gccgatagcg cccaggcgca ggctgcgcgc gcgaagttca cgtatgcgcc ggcgaaccgg ccgccgacgg tgatccagtg cgatacgtcg tcgggcaaca cgtggttctc gggcacgctg atcggcgagc gcgcgcgtca gtggaccaag atcctgacgg acggcaaggg cacctactgc atgaccgcgc aggaggacaa cgcgacgtat gaagcgctca agcgcgcggc gagcgtgaag cgggtcgacc tgtcgcgtgt ggccgtgctg cgcaccgggt ccgatttcga tcggccgtac gcggggcaga cgagcaccga caacctgctg aactacgcgg accagggcgg cttcgccccg gcgaccgaaa acctgtaccg cgcgggcaat ccgctggtgc aggacatcgt cacgcactgg ggcgaatggc gcgacggcgt gccgcgtcgt tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat