BuceA.18593.a

Putative type VI secretion system protein TssF

CENTER ID: BuceA.18593.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18593.a.B1.GE37124 full length 1 611
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.362
RefSeq: YP_002229511.1
UniProt: B4E6R7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEELLPYYER ELSFLRRYSR DFAERYPKIA ARLALSGEHC EDPHVERMIE SFALLGARIN KKLDDDYPEF TEALLEVLYP HYLRPFPSCS IAQFTPASPG QQTEPVVIER GTELKSRPIR GVQCRFRTAY DVTLAPIRIS EARYTPVALA PSATVLPSNA TGVISITFES LAAQLDLGAL KLSTLRAHLH GEQSFVAALT DCLFVNVLGA YVEPERNGRW TALRKLPIAQ AGFAEDDALI DYPAKSHPAY RLLTEYFAFP DKFDFVDFDL AAIARASGRC QRATLHLVLQ DVRSDSHVAR LLELLMASHF RLFCTPIVNL FRQHGEPIRI THRAVSYPVI AEARRAFAYE VYSIDSVKLV RQQAHEESVI EFRPFYSLHH GEAARIGHYW FARRNDWVAQ KSPGYETEIS IVDIDFEPTS PQTDTLSLDL TCTNRDLPAM LAFGLEGGDL FQEGGAQTSG ISLLRRPTQS VRFERGRAAH WRLVSHLALN HVSLVAHGLA PLKEMLTLYD LRRTAVSMRQ IDGLVGVEQR GAVQWLPGKP FATFVRGIEI RLTIDEEHFV GASLASFVRV LDSFFGLYVH LNSFVQLVVV SKRTGEEIIR CKPRTGESIL A
NT Sequence
atggaagaat tgctgccgta ttacgaacgc gaattatcgt ttttgcggcg ctattcgcga gatttcgccg aacgctatcc gaagatcgcc gcgcgcctcg cgctgtccgg cgagcattgc gaggatccgc acgtcgagcg gatgatcgag tcgttcgcgc tgctcggcgc gcgcatcaac aagaagctcg acgacgacta ccccgaattc accgaagcgc tgctggaagt gctgtatccg cactacctgc ggccgttccc gtcgtgttcg atcgcgcagt tcacgccggc ttcgcccggc cagcagaccg aaccggtcgt gatcgagcgc ggcaccgagc tgaagagccg tccgatccgc ggcgtgcaat gccggttccg taccgcctac gacgtgacgc tcgcgccgat ccgcatctcg gaagcacgct atacgccggt cgcgctcgcg ccgagcgcga cggtgctgcc gtcgaacgcg acgggcgtga tctcgatcac gttcgaatcg ctcgccgcgc agctcgatct cggcgcgctg aagctgtcga cgctgcgcgc gcacctgcac ggcgagcagt cgttcgtcgc cgcgctcacc gactgcctgt tcgtcaacgt gctcggcgcg tacgtcgagc ccgaacgcaa cggccgctgg accgcgctgc gcaagctgcc gatcgcgcag gccggcttcg ccgaggacga cgcgctgatc gactatccgg cgaaatcgca tcccgcatac cggctgctca ccgaatactt cgcgttcccc gacaagttcg atttcgtcga cttcgacctc gcggcgatcg cgcgcgcgtc gggccgctgc cagcgcgcga cgctgcatct ggtgctgcag gacgtgcgca gcgattcgca cgtcgcgcgc ctgctcgaat tgctgatggc gagccatttc cggctgttct gcacgccgat cgtgaacctg ttccgccagc acggcgagcc gatccgcatc acgcaccgcg cggtgtcgta tccggtgatc gccgaggcgc ggcgcgcgtt cgcgtacgag gtgtactcga tcgactcggt gaagctcgtc cggcagcagg cgcacgagga atcggtgatc gagttccggc cgttctattc gctgcatcac ggcgaagcgg cgcggatcgg ccactactgg ttcgcgcggc gcaacgactg ggtcgcacag aagagccccg gctacgaaac cgagatctcg atcgtcgaca tcgacttcga gccgacgtcg ccgcagaccg acacgctgag cctcgatctc acctgcacga accgcgacct gcctgccatg ctggcgttcg gcctcgaagg cggcgacctg ttccaggaag gcggcgcgca gacgagcggc atttcgctgc tgcgtcgccc gacgcagagc gtgcgcttcg agcgcggccg cgccgcgcac tggcggctcg tgtcgcacct cgcgctcaac cacgtatcgc tcgtcgcgca cggcctcgca ccgctgaagg aaatgctgac gctgtacgac ctgcgacgca cggcggtgtc gatgcgccag atcgacggcc tggtcggcgt cgagcagcgc ggcgccgtgc agtggctgcc cggcaagccg ttcgcgacct tcgtgcgcgg catcgagatc cggctgacga tcgacgagga acacttcgtc ggcgcgagcc tcgcgtcctt cgtgcgcgtg ctcgacagct tcttcgggct gtacgtccac ctcaacagtt tcgttcaatt ggtcgtcgtg tcgaagcgca ccggcgagga gatcattcga tgcaagcccc gaaccggcga atcgatcctg gcgtag
Details for BuceA.18593.a.B1.GE37124
HARVESTED ON: 7/17/2013
SEQUENCED ON: 3/27/2014
EXPECTED MW: 70kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHME ELLPYYEREL SFLRRYSRDF AERYPKIAAR LALSGEHCED PHVERMIESF ALLGARINKK LDDDYPEFTE ALLEVLYPHY LRPFPSCSIA QFTPASPGQQ TEPVVIERGT ELKSRPIRGV QCRFRTAYDV TLAPIRISEA RYTPVALAPS ATVLPSNATG VISITFESLA AQLDLGALKL STLRAHLHGE QSFVAALTDC LFVNVLGAYV EPERNGRWTA LRKLPIAXAG FAXDDALIDY PAKSHPAYRL LTEYFAFPDK FDFVDFDLAA IARASGRCQR ATLHLVLQDV RSDSHVARLL ELLXASHFRL FCTPIVNXFR QHGEPIRITH RAVSYPVIAE ARRAFAYEVY SIDSVKLVRQ QAHEESVIEF RPFYSLHHGE AARIGHYWFA RRNDWVAQKS PGYETEISIV DIDFEPTSPQ TDTLSLDLTC TNRDLPAMLA FGLEGGDLFQ EGGAQTSGIS LLRRPTQSVR FERGRAAHWR LVSHLALNHV SLVAHGLAPL KEMLTLYDLR RTAVSMRQID GLVGVEQRGA VQWLPGKPFA TFVRGIEIRL TIDEEHFVGA SLASFVRVLD SFFGLYVHLN SFVQLVVVSK RTGEEIIR
Validated NT Sequence
atggctcacc accaccacca ccatatggaa gaattgctgc cgtattacga acgcgaatta tcgtttttgc ggcgctattc gcgagatttc gccgaacgct atccgaagat cgccgcgcgc ctcgcgctgt ccggcgagca ttgcgaggat ccgcacgtcg agcggatgat cgagtcgttc gcgctgctcg gcgcgcgcat caacaagaag ctcgacgacg actaccccga attcaccgaa gcgctgctgg aagtgctgta tccgcactac ctgcggccgt tcccgtcgtg ttcgatcgcg cagttcacgc cggcttcgcc cggccagcag accgaaccgg tcgtgatcga gcgcggcacc gagctgaaga gccgtccgat ccgcggcgtg caatgccggt tccgtaccgc ctacgacgtg acgctcgcgc cgatccgcat ctcggaagca cgctatacgc cggtcgcgct cgcgccgagc gcgacggtgc tgccgtcgaa cgcgacgggc gtgatctcga tcacgttcga atcgctcgcc gcgcagctcg atctcggcgc gctgaagctg tcgacgctgc gcgcgcacct gcacggcgag cagtcgttcg tcgccgcgct caccgactgc ctgttcgtca acgtgctcgg cgcgtacgtc gagcccgaac gcaacggccg ctggaccgcg ctgcgcaagc tgccgatcgc gcangccggc ttcgccgang acgacgcgct gatcgactat ccggcgaaat cgcatccngc ataccggctg ctcaccgaat acttcgcgtt ccccgacaag ttcgatttcg tcgacttcga cctcgcggcg atcgcgcgcg cgtcgggccg ctgccagcgc gcgacgctgc atctggtgct gcaggacgtg cgcagcgatt cgcacgtcgc gcgcctgctc gaattgctga nggcgagcca tttccggctg ttctgcacgc cgatcgtgaa cntgttccgc cagcacggcg agccgatccg catcacgcac cgcgcggtgt cgtatccggt gatcgccgag gcgcggcgcg cgttcgcgta cgaggtgtac tcgatcgact cggtgaagct cgtccggcag caggcgcacg aggaatcggt gatcgagttc cggccgttct attcgctgca tcacggcgaa gcggcgcgga tcggccacta ctggttcgcg cggcgcaacg actgggtcgc acagaagagc cccggctacg aaaccgagat ctcgatcgtc gacatcgact tcgagccgac gtcgccgcag accgacacgc tgagcctcga tctcacctgc acgaaccgcg acctgcctgc catgctggcg ttcggcctcg aaggcggcga cctgttccag gaaggcggcg cgcagacgag cggcatttcg ctgctgcgtc gcccgacgca gagcgtgcgc ttcgagcgcg gccgcgccgc gcactggcgg ctcgtgtcgc acctcgcgct caaccacgta tcgctcgtcg cgcacggcct cgcaccgctg aaggaaatgc tgacgctgta cgacctgcga cgcacggcgg tgtcgatgcg ccagatcgac ggcctggtcg gcgtcgagca gcgcggcgcc gtgcagtggc tgcccggcaa gccgttcgcg accttcgtgc gcggcatcga gatccggctg acgatcgacg aggaacactt cgtcggcgcg agcctcgcgt ccttcgtgcg cgtgctcgac agcttcttcg ggctgtacgt ccacctcaac agtttcgttc aattggtcgt cgtgtcgaag cgcaccggcg aggagatcat tcgatgc
Expected Protein Sequence
MAHHHHHHME ELLPYYEREL SFLRRYSRDF AERYPKIAAR LALSGEHCED PHVERMIESF ALLGARINKK LDDDYPEFTE ALLEVLYPHY LRPFPSCSIA QFTPASPGQQ TEPVVIERGT ELKSRPIRGV QCRFRTAYDV TLAPIRISEA RYTPVALAPS ATVLPSNATG VISITFESLA AQLDLGALKL STLRAHLHGE QSFVAALTDC LFVNVLGAYV EPERNGRWTA LRKLPIAQAG FAEDDALIDY PAKSHPAYRL LTEYFAFPDK FDFVDFDLAA IARASGRCQR ATLHLVLQDV RSDSHVARLL ELLMASHFRL FCTPIVNLFR QHGEPIRITH RAVSYPVIAE ARRAFAYEVY SIDSVKLVRQ QAHEESVIEF RPFYSLHHGE AARIGHYWFA RRNDWVAQKS PGYETEISIV DIDFEPTSPQ TDTLSLDLTC TNRDLPAMLA FGLEGGDLFQ EGGAQTSGIS LLRRPTQSVR FERGRAAHWR LVSHLALNHV SLVAHGLAPL KEMLTLYDLR RTAVSMRQID GLVGVEQRGA VQWLPGKPFA TFVRGIEIRL TIDEEHFVGA SLASFVRVLD SFFGLYVHLN SFVQLVVVSK RTGEEIIRCK PRTGESILA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag aattgctgcc gtattacgaa cgcgaattat cgtttttgcg gcgctattcg cgagatttcg ccgaacgcta tccgaagatc gccgcgcgcc tcgcgctgtc cggcgagcat tgcgaggatc cgcacgtcga gcggatgatc gagtcgttcg cgctgctcgg cgcgcgcatc aacaagaagc tcgacgacga ctaccccgaa ttcaccgaag cgctgctgga agtgctgtat ccgcactacc tgcggccgtt cccgtcgtgt tcgatcgcgc agttcacgcc ggcttcgccc ggccagcaga ccgaaccggt cgtgatcgag cgcggcaccg agctgaagag ccgtccgatc cgcggcgtgc aatgccggtt ccgtaccgcc tacgacgtga cgctcgcgcc gatccgcatc tcggaagcac gctatacgcc ggtcgcgctc gcgccgagcg cgacggtgct gccgtcgaac gcgacgggcg tgatctcgat cacgttcgaa tcgctcgccg cgcagctcga tctcggcgcg ctgaagctgt cgacgctgcg cgcgcacctg cacggcgagc agtcgttcgt cgccgcgctc accgactgcc tgttcgtcaa cgtgctcggc gcgtacgtcg agcccgaacg caacggccgc tggaccgcgc tgcgcaagct gccgatcgcg caggccggct tcgccgagga cgacgcgctg atcgactatc cggcgaaatc gcatcccgca taccggctgc tcaccgaata cttcgcgttc cccgacaagt tcgatttcgt cgacttcgac ctcgcggcga tcgcgcgcgc gtcgggccgc tgccagcgcg cgacgctgca tctggtgctg caggacgtgc gcagcgattc gcacgtcgcg cgcctgctcg aattgctgat ggcgagccat ttccggctgt tctgcacgcc gatcgtgaac ctgttccgcc agcacggcga gccgatccgc atcacgcacc gcgcggtgtc gtatccggtg atcgccgagg cgcggcgcgc gttcgcgtac gaggtgtact cgatcgactc ggtgaagctc gtccggcagc aggcgcacga ggaatcggtg atcgagttcc ggccgttcta ttcgctgcat cacggcgaag cggcgcggat cggccactac tggttcgcgc ggcgcaacga ctgggtcgca cagaagagcc ccggctacga aaccgagatc tcgatcgtcg acatcgactt cgagccgacg tcgccgcaga ccgacacgct gagcctcgat ctcacctgca cgaaccgcga cctgcctgcc atgctggcgt tcggcctcga aggcggcgac ctgttccagg aaggcggcgc gcagacgagc ggcatttcgc tgctgcgtcg cccgacgcag agcgtgcgct tcgagcgcgg ccgcgccgcg cactggcggc tcgtgtcgca cctcgcgctc aaccacgtat cgctcgtcgc gcacggcctc gcaccgctga aggaaatgct gacgctgtac gacctgcgac gcacggcggt gtcgatgcgc cagatcgacg gcctggtcgg cgtcgagcag cgcggcgccg tgcagtggct gcccggcaag ccgttcgcga ccttcgtgcg cggcatcgag atccggctga cgatcgacga ggaacacttc gtcggcgcga gcctcgcgtc cttcgtgcgc gtgctcgaca gcttcttcgg gctgtacgtc cacctcaaca gtttcgttca attggtcgtc gtgtcgaagc gcaccggcga ggagatcatt cgatgcaagc cccgaaccgg cgaatcgatc ctggcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t