SSGCID: Target Page

BuceA.18588.a

Putative exported protein

CENTER ID:	BuceA.18588.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18588.a.B1.GE37123	full length		1	246

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2763
RefSeq:	YP_002231772.1
UniProt:	B4E8G2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKKSALALS LALAAAVPVA LTLASPAAHA QTANPHFPEP AGVLSLSSQA SADVPQDIIH ITLFYEQQAK DPGSLTAQLN QRADAALSQA KGVSGVTAHT GAFSVYPSTD RDGKISAWRG RTEVALESRD FAAASKLAGQ LSNLMQVANV EFSLSPEAQR AAEQKLTTEA IKSFRARADE AAKAFGYSSY TIRDVNVGSG RNVQPYTRMM AMAAAPMDSA KMSAPIAVEG GKATVSVTVN GSVQMK

NT Sequence

atgaccaaga aatccgcact cgcgctgtcg ctcgcccttg ccgccgccgt tcccgtcgcg ctgacgctcg cgtcgcccgc cgcgcacgcc cagacggcga acccgcactt tccggagccg gcgggcgtgc tgtcgctttc gtcgcaggcc agtgccgacg tgccgcagga catcattcac atcacgctgt tctacgagca gcaggccaag gatccgggca gcctcaccgc gcagctgaac cagcgcgcgg acgcggcgct gtcgcaggcg aagggcgtgt cgggcgtcac cgcgcatacg ggtgcgttct ccgtgtatcc gagcaccgat cgcgacggca agatctccgc atggcgcggc cgcaccgagg tcgcgctcga atcgcgcgat ttcgccgcgg cgtcgaagct ggcaggccag ttgtcgaacc tgatgcaggt cgcgaacgtc gagttctcgt tgtcgccgga agcgcagcgc gcggccgagc aaaagctcac gacggaagcg atcaagtcgt tccgcgcccg cgcggacgaa gccgcgaagg cgttcggcta cagcagctac acgatccgcg acgtgaacgt cggcagcggc cgcaacgtgc agccgtacac gcgcatgatg gcgatggccg cggcgccgat ggacagcgcg aagatgagcg cgccgatcgc ggtcgaaggc ggcaaggcca ccgtgtcggt caccgtcaac ggctccgtgc agatgaagta a

Details for BuceA.18588.a.B1.GE37123

HARVESTED ON:	7/17/2013
SEQUENCED ON:	7/20/2013
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT KKSALALSLA LAAAVPVALT LASPAAHAQT ANPHFPEPAG VLSLSSQASA DVPQDIIHIT LFYEQQAKDP GSLTAQLNQR ADAALSQAKG VSGVTAHTGA FSVYPSTDRD GKISAWRGRT EVALESRDFA AASKLAGQLS NLMQVANVEF SLSPEAQRAA EQKLTTEAIK SFRARADEAA KAFGYSSYTI RDVNVGSGRN VQPYTRMMAM AAAPMDSAKM SAPIAVEGGK ATVSVTVNGS VQMK

Validated NT Sequence

atcttactca cttacttcat ctgcacggag ccgttgacgg tgaccgacac ggtggccttg ccgccttcga ccgcgatcgg cgcgctcatc ttcgcgctgt ccatcggcgc cgcggccatc gccatcatgc gcgtgtacgg ctgcacgttg cggccgctgc cgacgttcac gtcgcggatc gtgtagctgc tgtagccgaa cgccttcgcg gcttcgtccg cgcgggcgcg gaacgacttg atcgcttccg tcgtgagctt ttgctcggcc gcgcgctgcg cttccggcga caacgagaac tcgacgttcg cgacctgcat caggttcgac aactggcctg ccagcttcga cgccgcggcg aaatcgcgcg attcgagcgc gacctcggtg cggccgcgcc atgcggagat cttgccgtcg cgatcggtgc tcggatacac ggagaacgca cccgtatgcg cggtgacgcc cgacacgccc ttcgcctgcg acagcgccgc gtccgcgcgc tggttcagct gcgcggtgag gctgcccgga tccttggcct gctgctcgta gaacagcgtg atgtgaatga tgtcctgcgg cacgtcggca ctggcctgcg acgaaagcga cagcacgccc gccggctccg gaaagtgcgg gttcgccgtc tgggcgtgcg cggcgggcga cgcgagcgtc agcgcgacgg gaacggcggc ggcaagggcg agcgacagcg cgagtgcgga tttcttggtc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca agaaatccgc actcgcgctg tcgctcgccc ttgccgccgc cgttcccgtc gcgctgacgc tcgcgtcgcc cgccgcgcac gcccagacgg cgaacccgca ctttccggag ccggcgggcg tgctgtcgct ttcgtcgcag gccagtgccg acgtgccgca ggacatcatt cacatcacgc tgttctacga gcagcaggcc aaggatccgg gcagcctcac cgcgcagctg aaccagcgcg cggacgcggc gctgtcgcag gcgaagggcg tgtcgggcgt caccgcgcat acgggtgcgt tctccgtgta tccgagcacc gatcgcgacg gcaagatctc cgcatggcgc ggccgcaccg aggtcgcgct cgaatcgcgc gatttcgccg cggcgtcgaa gctggcaggc cagttgtcga acctgatgca ggtcgcgaac gtcgagttct cgttgtcgcc ggaagcgcag cgcgcggccg agcaaaagct cacgacggaa gcgatcaagt cgttccgcgc ccgcgcggac gaagccgcga aggcgttcgg ctacagcagc tacacgatcc gcgacgtgaa cgtcggcagc ggccgcaacg tgcagccgta cacgcgcatg atggcgatgg ccgcggcgcc gatggacagc gcgaagatga gcgcgccgat cgcggtcgaa ggcggcaagg ccaccgtgtc ggtcaccgtc aacggctccg tgcagatgaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat