BuceA.18584.a
Orotate phosphoribosyltransferase (OPRT) (OPRTase) (EC 2.4.2.10)
| CENTER ID: | BuceA.18584.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.18584.a.B1.GE37072 | full length | 1 | 228 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.18584.a.B1.PS01911 | full length | 1 | 228 |
Structures
4OHC
DEPOSITED: 1/17/2014
DETERMINATION: XRay
CLONE: BuceA.18584.a.B1.GE37072
PROTEIN: BuceA.18584.a.B1.PS01911
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216591.5.peg.213 |
| RefSeq: | YP_002229370.1 |
| UniProt: | B4E589 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGYDRQSIS DTTAKILLEV QAVHFNAEKP FIFTSGWASP VYIDCRKLIS YPRVRRALME MAETTITRDI GFEQIDAVAG GETAGIPFAA WIADRMMVPM QYVRKKPKGF GRNAQIEGHL EEGSRVLLVE DLTTDSRSKI NFVNALRTAG ATVNHCFVLF HYNIFKESVS VLKDIDVDLH ALATWWDVLR VAKASGYFET KTLDEVEKFL HAPAEWSAAH GGATAPKE
NT Sequence
atgacaggct acgatcgtca gtcgatctcg gatacgaccg ccaaaatcct gctcgaagtg caggcggtgc acttcaatgc cgaaaaaccg ttcatcttca cgtccggctg ggcaagcccc gtctatatcg actgccgcaa gctgatctcg tatccgcgcg tgcgccgtgc gctgatggaa atggcggaaa cgacgatcac gcgcgacatc ggcttcgagc agatcgacgc ggtggcgggc ggcgagacgg ccggcatccc gttcgcggca tggatcgcgg accggatgat ggtgccgatg cagtacgtgc gcaaaaagcc gaagggtttc ggccgcaatg cgcagatcga aggccatctc gaggaaggct cgcgcgtgct gctggtggaa gacctgacca ccgacagccg cagcaagatc aacttcgtca acgcgctgcg caccgcgggc gcgacggtga accactgctt cgtgctgttc cactacaaca tcttcaagga aagcgtgtcg gtcctgaagg acatcgacgt cgacctgcac gcgctcgcga cgtggtggga cgtgctgcgc gtcgcgaagg cgtcgggcta cttcgagacg aagacgctcg acgaagtcga gaaattcctg cacgcaccgg ccgaatggtc ggccgcgcac ggcggcgcga cggccccgaa ggaatga
Details for BuceA.18584.a.B1.GE37072
| HARVESTED ON: | 6/12/2013 |
| SEQUENCED ON: | 6/11/2013 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT GYDRQSISDT TAKILLEVQA VHFNAEKPFI FTSGWASPVY IDCRKLISYP RVRRALMEMA ETTITRDIGF EQIDAVAGGE TAGIPFAAWI ADRMMVPMQY VRKKPKGFGR NAQIEGHLEE GSRVLLVEDL TTDSRSKINF VNALRTAGAT VNHCFVLFHY NIFKESVSVL KDIDVDLHAL ATWWDVLRVA KASGYFETKT LDEVEKFLHA PAEWSAAHGG ATAPKE
Validated NT Sequence
gttagcagcc ggatcctatc ttactcactt attccttcgg ggccgtcgcg ccgccgtgcg cggccgacca ttcggccggt gcgtgcagga atttctcgac ttcgtcgagc gtcttcgtct cgaagtagcc cgacgccttc gcgacgcgca gcacgtccca ccacgtcgcg agcgcgtgca ggtcgacgtc gatgtccttc aggaccgaca cgctttcctt gaagatgttg tagtggaaca gcacgaagca gtggttcacc gtcgcgcccg cggtgcgcag cgcgttgacg aagttgatct tgctgcggct gtcggtggtc aggtcttcca ccagcagcac gcgcgagcct tcctcgagat ggccttcgat ctgcgcattg cggccgaaac ccttcggctt tttgcgcacg tactgcatcg gcaccatcat ccggtccgcg atccatgccg cgaacgggat gccggccgtc tcgccgcccg ccaccgcgtc gatctgctcg aagccgatgt cgcgcgtgat cgtcgtttcc gccatttcca tcagcgcacg gcgcacgcgc ggatacgaga tcagcttgcg gcagtcgata tagacggggc ttgcccagcc ggacgtgaag atgaacggtt tttcggcatt gaagtgcacc gcctgcactt cgagcaggat tttggcggtc gtatccgaga tcgactgacg atcgtagcct gtcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT GYDRQSISDT TAKILLEVQA VHFNAEKPFI FTSGWASPVY IDCRKLISYP RVRRALMEMA ETTITRDIGF EQIDAVAGGE TAGIPFAAWI ADRMMVPMQY VRKKPKGFGR NAQIEGHLEE GSRVLLVEDL TTDSRSKINF VNALRTAGAT VNHCFVLFHY NIFKESVSVL KDIDVDLHAL ATWWDVLRVA KASGYFETKT LDEVEKFLHA PAEWSAAHGG ATAPKE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacag gctacgatcg tcagtcgatc tcggatacga ccgccaaaat cctgctcgaa gtgcaggcgg tgcacttcaa tgccgaaaaa ccgttcatct tcacgtccgg ctgggcaagc cccgtctata tcgactgccg caagctgatc tcgtatccgc gcgtgcgccg tgcgctgatg gaaatggcgg aaacgacgat cacgcgcgac atcggcttcg agcagatcga cgcggtggcg ggcggcgaga cggccggcat cccgttcgcg gcatggatcg cggaccggat gatggtgccg atgcagtacg tgcgcaaaaa gccgaagggt ttcggccgca atgcgcagat cgaaggccat ctcgaggaag gctcgcgcgt gctgctggtg gaagacctga ccaccgacag ccgcagcaag atcaacttcg tcaacgcgct gcgcaccgcg ggcgcgacgg tgaaccactg cttcgtgctg ttccactaca acatcttcaa ggaaagcgtg tcggtcctga aggacatcga cgtcgacctg cacgcgctcg cgacgtggtg ggacgtgctg cgcgtcgcga aggcgtcggg ctacttcgag acgaagacgc tcgacgaagt cgagaaattc ctgcacgcac cggccgaatg gtcggccgcg cacggcggcg cgacggcccc gaaggaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuceA.18584.a.B1.PS01911
| PURIFICATION DATe: | 10/8/2013 |
| CONCENTRATION: | 14mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT GYDRQSISDT TAKILLEVQA VHFNAEKPFI FTSGWASPVY IDCRKLISYP RVRRALMEMA ETTITRDIGF EQIDAVAGGE TAGIPFAAWI ADRMMVPMQY VRKKPKGFGR NAQIEGHLEE GSRVLLVEDL TTDSRSKINF VNALRTAGAT VNHCFVLFHY NIFKESVSVL KDIDVDLHAL ATWWDVLRVA KASGYFETKT LDEVEKFLHA PAEWSAAHGG ATAPKE
Validated NT Sequence
gttagcagcc ggatcctatc ttactcactt attccttcgg ggccgtcgcg ccgccgtgcg cggccgacca ttcggccggt gcgtgcagga atttctcgac ttcgtcgagc gtcttcgtct cgaagtagcc cgacgccttc gcgacgcgca gcacgtccca ccacgtcgcg agcgcgtgca ggtcgacgtc gatgtccttc aggaccgaca cgctttcctt gaagatgttg tagtggaaca gcacgaagca gtggttcacc gtcgcgcccg cggtgcgcag cgcgttgacg aagttgatct tgctgcggct gtcggtggtc aggtcttcca ccagcagcac gcgcgagcct tcctcgagat ggccttcgat ctgcgcattg cggccgaaac ccttcggctt tttgcgcacg tactgcatcg gcaccatcat ccggtccgcg atccatgccg cgaacgggat gccggccgtc tcgccgcccg ccaccgcgtc gatctgctcg aagccgatgt cgcgcgtgat cgtcgtttcc gccatttcca tcagcgcacg gcgcacgcgc ggatacgaga tcagcttgcg gcagtcgata tagacggggc ttgcccagcc ggacgtgaag atgaacggtt tttcggcatt gaagtgcacc gcctgcactt cgagcaggat tttggcggtc gtatccgaga tcgactgacg atcgtagcct gtcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT GYDRQSISDT TAKILLEVQA VHFNAEKPFI FTSGWASPVY IDCRKLISYP RVRRALMEMA ETTITRDIGF EQIDAVAGGE TAGIPFAAWI ADRMMVPMQY VRKKPKGFGR NAQIEGHLEE GSRVLLVEDL TTDSRSKINF VNALRTAGAT VNHCFVLFHY NIFKESVSVL KDIDVDLHAL ATWWDVLRVA KASGYFETKT LDEVEKFLHA PAEWSAAHGG ATAPKE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAG GCTACGATCG TCAGTCGATC TCGGATACGA CCGCCAAAAT CCTGCTCGAA GTGCAGGCGG TGCACTTCAA TGCCGAAAAA CCGTTCATCT TCACGTCCGG CTGGGCAAGC CCCGTCTATA TCGACTGCCG CAAGCTGATC TCGTATCCGC GCGTGCGCCG TGCGCTGATG GAAATGGCGG AAACGACGAT CACGCGCGAC ATCGGCTTCG AGCAGATCGA CGCGGTGGCG GGCGGCGAGA CGGCCGGCAT CCCGTTCGCG GCATGGATCG CGGACCGGAT GATGGTGCCG ATGCAGTACG TGCGCAAAAA GCCGAAGGGT TTCGGCCGCA ATGCGCAGAT CGAAGGCCAT CTCGAGGAAG GCTCGCGCGT GCTGCTGGTG GAAGACCTGA CCACCGACAG CCGCAGCAAG ATCAACTTCG TCAACGCGCT GCGCACCGCG GGCGCGACGG TGAACCACTG CTTCGTGCTG TTCCACTACA ACATCTTCAA GGAAAGCGTG TCGGTCCTGA AGGACATCGA CGTCGACCTG CACGCGCTCG CGACGTGGTG GGACGTGCTG CGCGTCGCGA AGGCGTCGGG CTACTTCGAG ACGAAGACGC TCGACGAAGT CGAGAAATTC CTGCACGCAC CGGCCGAATG GTCGGCCGCG CACGGCGGCG CGACGGCCCC GAAGGAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT