BuceA.18583.a

Putative storage protein

CENTER ID: BuceA.18583.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18583.a.B1.GE37071 full length 1 194
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.871
RefSeq: YP_002229985.1
UniProt: B4EAU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVFAPEKLA ADYQSGIAAW FAIARPAIHG FEAVVELNLQ AARTALEEYE DKLKNAFNGN NPAAGFAQQV AAPQEAAGKV VSYGRHLFDI AVSTQSEWAK VAQAQYEQND KRLKDVLGEL SKHAPAGSAP VVAALNSALS AATAAADSVR AATGQAIEAA QSGFDAVSET ATRGAKQTAA AARKDAAAAR ESAA
NT Sequence
atgtccgtat ttgcccccga aaaactcgcc gccgattatc aatccggtat cgccgcctgg ttcgcgatcg cccgtccggc gatccacggt ttcgaggccg tcgtcgaact caacctgcaa gcggcccgca cggcgctcga ggaatacgag gacaagctga agaacgcgtt caacggcaac aacccggccg cgggcttcgc gcagcaggtc gccgcgccgc aggaagcggc cggcaaggtc gtgtcgtatg gccgccatct gttcgacatc gcggtgtcga cgcaatccga gtgggcgaag gtcgcgcagg cccagtacga acagaacgac aagcgcctga aggacgtgct cggcgaactg tcgaagcacg caccggccgg ctcggcgccg gtggtggccg cgctgaattc ggcgttgtcc gcggcgaccg ccgcggccga ctccgtgcgt gcagcgacgg ggcaggcgat cgaagcggcg cagagcggtt tcgatgcagt cagcgaaacg gccacgcgcg gtgccaagca gacggctgcg gccgcccgca aggacgccgc ggctgcgcgc gaatcggctg cgtga
Details for BuceA.18583.a.B1.GE37071
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM SVFAPEKLAA DYQSGIAAWF AIARPAIHGF EAVVELNLQA ARTALEEYED KLKNAFNGNN PAAGFAQQVA APQEAAGKVV SYGRHLFDIA VSTQSEWAKV AQAQYEQNDK RLKDVLGELS KHAPAGSAPV VAALNSALSA ATAAADSVRA ATGQAIEAAQ SGFDAVSETA TRGAKQTAAA ARKDAAAARE SAA
Validated NT Sequence
tttgnttact ttaagaagaa ganataccat ggctcaccac caccaccacc accatatgtc cgtatttgcc cccgaaaaac tcgccgccga ttatcaatcc ggtatcgccg cctggttcgc gatcgcccgt ccggcgatcc acggtttcga ggccgtcgtc gaactcaacc tgcaagcggc ccgcacggcg ctcgaggaat acgaggacaa gctgaagaac gcgttcaacg gcaacaaccc ggccgcgggc ttcgcgcagc aggtcgccgc gccgcaggaa gcggccggca aggtcgtgtc gtatggccgc catctgttcg acatcgcggt gtcgacgcaa tccgagtggg cgaaggtcgc gcaggcccag tacgaacaga acgacaagcg cctgaaggac gtgctcggcg aactgtcgaa gcacgcaccg gccggctcgg cgccggtggt ggccgcgctg aattcggcgt tgtccgcggc gaccgccgcg gccgactccg tgcgtgcagc gacggggcag gcgatcgaag cggcgcagag cggtttcgat gcagtcagcg aaacggccac gcgcggtgcc aagcagacgg ctgcggccgc ccgcaaggac gccgcggctg cgcgcgaatc ggctgcgtaa gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aannnnnnnn nnnnngnnnn aannnnnnnn nnnannannn nancggnngc ngcngncnan tcannnnnnn nnnnggntnt gnnnagnnnc cggnnccnnt cttantnant tnnnnnnnna tncgngngnn nccncggcgt ncntngcggg nggcgcnncn ntggnnngnn nnnnnnngnn nnntnntnnn ngnnnnaann nnnntnnnnn nnnannncng cnnnnnnnnn nnnnnnggag
Expected Protein Sequence
MAHHHHHHMS VFAPEKLAAD YQSGIAAWFA IARPAIHGFE AVVELNLQAA RTALEEYEDK LKNAFNGNNP AAGFAQQVAA PQEAAGKVVS YGRHLFDIAV STQSEWAKVA QAQYEQNDKR LKDVLGELSK HAPAGSAPVV AALNSALSAA TAAADSVRAA TGQAIEAAQS GFDAVSETAT RGAKQTAAAA RKDAAAARES AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg tatttgcccc cgaaaaactc gccgccgatt atcaatccgg tatcgccgcc tggttcgcga tcgcccgtcc ggcgatccac ggtttcgagg ccgtcgtcga actcaacctg caagcggccc gcacggcgct cgaggaatac gaggacaagc tgaagaacgc gttcaacggc aacaacccgg ccgcgggctt cgcgcagcag gtcgccgcgc cgcaggaagc ggccggcaag gtcgtgtcgt atggccgcca tctgttcgac atcgcggtgt cgacgcaatc cgagtgggcg aaggtcgcgc aggcccagta cgaacagaac gacaagcgcc tgaaggacgt gctcggcgaa ctgtcgaagc acgcaccggc cggctcggcg ccggtggtgg ccgcgctgaa ttcggcgttg tccgcggcga ccgccgcggc cgactccgtg cgtgcagcga cggggcaggc gatcgaagcg gcgcagagcg gtttcgatgc agtcagcgaa acggccacgc gcggtgccaa gcagacggct gcggccgccc gcaaggacgc cgcggctgcg cgcgaatcgg ctgcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat