BuceA.18569.a

Maltose-binding protein

CENTER ID: BuceA.18569.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18569.a.B1.GE37068 full length 1 415
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18569.a.B1.PS01904 full length 1 415

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3156
RefSeq: YP_002232151.1
UniProt: B4EBS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVRSIMGAL CAAGLMAGAV AAQAAENVTV LHWWTSGGES KAVGVLKDDL QKQGYVWKDF AVAGGAGAAA MTALKTKVIS GDSPSAAQIK GPLIQDWADQ GVLVNIDSAA GDWKQNLPPE IDKIIKYKGH TVAAPFSVHR VNWLYINKAA LDKVGAKVPT TWPEFFAVAD KLKAAGIQPV AMGGQPWQDL TLWEDVVLSQ GPAFYKKALV DLDQATLTSP QMLSVFDTVR KIQGYFDTGR NGRDWNLATA MVINGKAGMQ FMGDWAKGEF ENAGKKAGKD YVCAPVPGTA NSYTFNVDSF VFFQQKGEKN ATPGQLALAK TIMTPDFQEQ FSLLKGSVPV RLGVKMDKFD DCAKKSYADE QTAIKSGGFV PSLAHGMAQG DATAGAITDV VTKFMNSQQD SKSAVAALAK AAKVK
NT Sequence
atgaaagttc gctcgatcat gggcgcgctc tgcgccgcgg gtctgatggc tggcgccgtg gcggcgcagg cagccgagaa cgtaaccgtg ctgcactggt ggacctcggg cggcgagtcg aaggccgtcg gcgtgctgaa ggacgacctg cagaagcagg gctacgtgtg gaaggacttc gcggtcgcgg gcggcgctgg cgccgcggcg atgaccgcgc tgaagaccaa ggtgatcagc ggcgattccc cgtcggccgc gcagatcaag ggcccgctga tccaggactg ggctgaccag ggcgtgctcg tcaacatcga ttcggccgcc ggcgactgga agcagaacct gccgccggaa atcgacaaga tcatcaagta caagggccat acggtcgccg cgccgttctc ggtgcaccgc gtcaactggc tgtacatcaa caaggccgcg ctcgacaagg tcggcgcgaa ggtgccgacc acctggcccg aattcttcgc ggtggccgac aagctgaagg ccgcgggcat ccagccggtc gcgatgggcg gccagccgtg gcaggacctg acgctgtggg aagacgtcgt gctgtcgcag ggcccggcgt tctacaagaa ggcgctggtc gacctcgacc aggcgacgct gacgtcgccg cagatgctgt cggtgttcga cacggtgcgc aagatccagg gctacttcga caccggccgt aacggccgcg actggaacct cgcgaccgcg atggtcatca acggcaaggc cggcatgcag ttcatgggcg actgggcgaa gggcgaattc gaaaacgccg gcaagaaggc gggcaaggac tacgtctgcg cgccggtgcc gggcaccgcg aattcgtaca cgttcaacgt cgattcgttc gtgttcttcc agcagaaggg cgagaagaac gcgacgccgg gccagctcgc gctcgcgaag acgatcatga cgccggactt ccaggagcag ttcagcctgc tgaagggctc ggtgccggtg cgtctcggcg tgaagatgga caagttcgac gactgcgcga agaagtcgta tgccgacgag cagaccgcga tcaagtcggg cggcttcgtg ccgtcgctcg cgcacggcat ggcgcaaggc gacgcgaccg ccggcgcgat caccgacgtc gtgacgaagt tcatgaactc gcagcaggat tcgaagagcg cggtcgccgc gctcgcgaag gccgcgaagg tcaagtaa
Details for BuceA.18569.a.B1.GE37068
HARVESTED ON: 6/12/2013
SEQUENCED ON: 6/11/2013
EXPECTED MW: 45kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGALCAAGLM AGAVAAQAAE NVTVLHWWTS GGESKAVGVL KDDLQKQGYV WKDFAVAGGA GAAAMTALKT KVISGDSPSA AQIKGPLIQD WADQGVLVNI DSAAGDWKQN LPPEIDKIIK YKGHTVAAPF SVHRVNWLYI NKAALDKVGA KVPTTWPEFF AVADKLKAAG IQPVAMGGQP WQDLTLWEDV VLSQGPAFYK KALVDLDQAT LTSPQMLSVF DTVRKIQGYF DTGRNGRDWN LATAMVINGK AGMQFMGDWA KGEFENAGKK AGKDYVCAPV PGTANSYTFN VDSFVFFQQK GEKNATPGQL ALAKTIMTPD FQEQFSLLKG SVPVRLGVKM DKFDDCAKKS YADEQTAIKS GGFVPSLAHG MAQGDATAGA ITDVVTKFMN SQQDSKSAVA
Validated NT Sequence
cgcggcgacc gcgctcttcg aatcctgctg cgagttcatg aacttcgtca cgacgtcggt gatcgcgccg gcggtcgcgt cgccttgcgc catgccgtgc gcgagcgacg gcacgaagcc gcccgacttg atcgcggtct gctcgtcggc atacgacttc ttcgcgcagt cgtcgaactt gtccatcttc acgccgagac gcaccggcac cgagcccttc agcaggctga actgctcctg gaagtccggc gtcatgatcg tcttcgcgag cgcgagctgg cccggcgtcg cgttcttctc gcccttctgc tggaagaaca cgaacgaatc gacgttgaac gtgtacgaat tcgcggtgcc cggcaccggc gcgcagacgt agtccttgcc cgccttcttg ccggcgtttt cgaattcgcc cttcgcccag tcgcccatga actgcatgcc ggccttgccg ttgatgacca tcgcggtcgc gaggttccag tcgcggccgt tacggccggt gtcgaagtag ccctggatct tgcgcaccgt gtcgaacacc gacagcatct gcggcgacgt cagcgtcgcc tggtcgaggt cgaccagcgc cttcttgtag aacgccgggc cctgcgacag cacgacgtct tcccacagcg tcaggtcctg ccacggctgg ccgcccatcg cgaccggctg gatgcccgcg gccttcagct tgtcggccac cgcgaagaat tcgggccagg tggtcggcac cttcgcgccg accttgtcga gcgcggcctt gttgatgtac agccagttga cgcggtgcac cgagaacggc gcggcgaccg tatggccctt gtacttgatg atcttgtcga tttccggcgg caggttctgc ttccagtcgc cggcggccga atcgatgttg acgagcacgc cctggtcagc ccagtcctgg atcagcgggc ccttgatctg cgcggccgac ggggaatcgc cgctgatcac cttggtcttc agcgcggtca tcgccgcggc gccagcgccg cccgcgaccg cgaagtcctt ccacacgtag ccctgcttct gcaggtcgtc cttcagcacg ccgacggcct tcgactcgcc gcccgaggtc caccagtgca gcacggttac gttctcggct gcctgcgccg ccacggcgcc agccatcaga cccgcggcgc agagcgcgcc catgatcgag cg
Expected Protein Sequence
MAHHHHHHMK VRSIMGALCA AGLMAGAVAA QAAENVTVLH WWTSGGESKA VGVLKDDLQK QGYVWKDFAV AGGAGAAAMT ALKTKVISGD SPSAAQIKGP LIQDWADQGV LVNIDSAAGD WKQNLPPEID KIIKYKGHTV AAPFSVHRVN WLYINKAALD KVGAKVPTTW PEFFAVADKL KAAGIQPVAM GGQPWQDLTL WEDVVLSQGP AFYKKALVDL DQATLTSPQM LSVFDTVRKI QGYFDTGRNG RDWNLATAMV INGKAGMQFM GDWAKGEFEN AGKKAGKDYV CAPVPGTANS YTFNVDSFVF FQQKGEKNAT PGQLALAKTI MTPDFQEQFS LLKGSVPVRL GVKMDKFDDC AKKSYADEQT AIKSGGFVPS LAHGMAQGDA TAGAITDVVT KFMNSQQDSK SAVAALAKAA KVK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaag ttcgctcgat catgggcgcg ctctgcgccg cgggtctgat ggctggcgcc gtggcggcgc aggcagccga gaacgtaacc gtgctgcact ggtggacctc gggcggcgag tcgaaggccg tcggcgtgct gaaggacgac ctgcagaagc agggctacgt gtggaaggac ttcgcggtcg cgggcggcgc tggcgccgcg gcgatgaccg cgctgaagac caaggtgatc agcggcgatt ccccgtcggc cgcgcagatc aagggcccgc tgatccagga ctgggctgac cagggcgtgc tcgtcaacat cgattcggcc gccggcgact ggaagcagaa cctgccgccg gaaatcgaca agatcatcaa gtacaagggc catacggtcg ccgcgccgtt ctcggtgcac cgcgtcaact ggctgtacat caacaaggcc gcgctcgaca aggtcggcgc gaaggtgccg accacctggc ccgaattctt cgcggtggcc gacaagctga aggccgcggg catccagccg gtcgcgatgg gcggccagcc gtggcaggac ctgacgctgt gggaagacgt cgtgctgtcg cagggcccgg cgttctacaa gaaggcgctg gtcgacctcg accaggcgac gctgacgtcg ccgcagatgc tgtcggtgtt cgacacggtg cgcaagatcc agggctactt cgacaccggc cgtaacggcc gcgactggaa cctcgcgacc gcgatggtca tcaacggcaa ggccggcatg cagttcatgg gcgactgggc gaagggcgaa ttcgaaaacg ccggcaagaa ggcgggcaag gactacgtct gcgcgccggt gccgggcacc gcgaattcgt acacgttcaa cgtcgattcg ttcgtgttct tccagcagaa gggcgagaag aacgcgacgc cgggccagct cgcgctcgcg aagacgatca tgacgccgga cttccaggag cagttcagcc tgctgaaggg ctcggtgccg gtgcgtctcg gcgtgaagat ggacaagttc gacgactgcg cgaagaagtc gtatgccgac gagcagaccg cgatcaagtc gggcggcttc gtgccgtcgc tcgcgcacgg catggcgcaa ggcgacgcga ccgccggcgc gatcaccgac gtcgtgacga agttcatgaa ctcgcagcag gattcgaaga gcgcggtcgc cgcgctcgcg aaggccgcga aggtcaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuceA.18569.a.B1.PS01904
PURIFICATION DATe: 9/26/2013
CONCENTRATION: 33.44mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGALCAAGLM AGAVAAQAAE NVTVLHWWTS GGESKAVGVL KDDLQKQGYV WKDFAVAGGA GAAAMTALKT KVISGDSPSA AQIKGPLIQD WADQGVLVNI DSAAGDWKQN LPPEIDKIIK YKGHTVAAPF SVHRVNWLYI NKAALDKVGA KVPTTWPEFF AVADKLKAAG IQPVAMGGQP WQDLTLWEDV VLSQGPAFYK KALVDLDQAT LTSPQMLSVF DTVRKIQGYF DTGRNGRDWN LATAMVINGK AGMQFMGDWA KGEFENAGKK AGKDYVCAPV PGTANSYTFN VDSFVFFQQK GEKNATPGQL ALAKTIMTPD FQEQFSLLKG SVPVRLGVKM DKFDDCAKKS YADEQTAIKS GGFVPSLAHG MAQGDATAGA ITDVVTKFMN SQQDSKSAVA
Validated NT Sequence
cgcggcgacc gcgctcttcg aatcctgctg cgagttcatg aacttcgtca cgacgtcggt gatcgcgccg gcggtcgcgt cgccttgcgc catgccgtgc gcgagcgacg gcacgaagcc gcccgacttg atcgcggtct gctcgtcggc atacgacttc ttcgcgcagt cgtcgaactt gtccatcttc acgccgagac gcaccggcac cgagcccttc agcaggctga actgctcctg gaagtccggc gtcatgatcg tcttcgcgag cgcgagctgg cccggcgtcg cgttcttctc gcccttctgc tggaagaaca cgaacgaatc gacgttgaac gtgtacgaat tcgcggtgcc cggcaccggc gcgcagacgt agtccttgcc cgccttcttg ccggcgtttt cgaattcgcc cttcgcccag tcgcccatga actgcatgcc ggccttgccg ttgatgacca tcgcggtcgc gaggttccag tcgcggccgt tacggccggt gtcgaagtag ccctggatct tgcgcaccgt gtcgaacacc gacagcatct gcggcgacgt cagcgtcgcc tggtcgaggt cgaccagcgc cttcttgtag aacgccgggc cctgcgacag cacgacgtct tcccacagcg tcaggtcctg ccacggctgg ccgcccatcg cgaccggctg gatgcccgcg gccttcagct tgtcggccac cgcgaagaat tcgggccagg tggtcggcac cttcgcgccg accttgtcga gcgcggcctt gttgatgtac agccagttga cgcggtgcac cgagaacggc gcggcgaccg tatggccctt gtacttgatg atcttgtcga tttccggcgg caggttctgc ttccagtcgc cggcggccga atcgatgttg acgagcacgc cctggtcagc ccagtcctgg atcagcgggc ccttgatctg cgcggccgac ggggaatcgc cgctgatcac cttggtcttc agcgcggtca tcgccgcggc gccagcgccg cccgcgaccg cgaagtcctt ccacacgtag ccctgcttct gcaggtcgtc cttcagcacg ccgacggcct tcgactcgcc gcccgaggtc caccagtgca gcacggttac gttctcggct gcctgcgccg ccacggcgcc agccatcaga cccgcggcgc agagcgcgcc catgatcgag cg
Expressed Protein Sequence
MAHHHHHHMK VRSIMGALCA AGLMAGAVAA QAAENVTVLH WWTSGGESKA VGVLKDDLQK QGYVWKDFAV AGGAGAAAMT ALKTKVISGD SPSAAQIKGP LIQDWADQGV LVNIDSAAGD WKQNLPPEID KIIKYKGHTV AAPFSVHRVN WLYINKAALD KVGAKVPTTW PEFFAVADKL KAAGIQPVAM GGQPWQDLTL WEDVVLSQGP AFYKKALVDL DQATLTSPQM LSVFDTVRKI QGYFDTGRNG RDWNLATAMV INGKAGMQFM GDWAKGEFEN AGKKAGKDYV CAPVPGTANS YTFNVDSFVF FQQKGEKNAT PGQLALAKTI MTPDFQEQFS LLKGSVPVRL GVKMDKFDDC AKKSYADEQT AIKSGGFVPS LAHGMAQGDA TAGAITDVVT KFMNSQQDSK SAVAALAKAA KVK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAG TTCGCTCGAT CATGGGCGCG CTCTGCGCCG CGGGTCTGAT GGCTGGCGCC GTGGCGGCGC AGGCAGCCGA GAACGTAACC GTGCTGCACT GGTGGACCTC GGGCGGCGAG TCGAAGGCCG TCGGCGTGCT GAAGGACGAC CTGCAGAAGC AGGGCTACGT GTGGAAGGAC TTCGCGGTCG CGGGCGGCGC TGGCGCCGCG GCGATGACCG CGCTGAAGAC CAAGGTGATC AGCGGCGATT CCCCGTCGGC CGCGCAGATC AAGGGCCCGC TGATCCAGGA CTGGGCTGAC CAGGGCGTGC TCGTCAACAT CGATTCGGCC GCCGGCGACT GGAAGCAGAA CCTGCCGCCG GAAATCGACA AGATCATCAA GTACAAGGGC CATACGGTCG CCGCGCCGTT CTCGGTGCAC CGCGTCAACT GGCTGTACAT CAACAAGGCC GCGCTCGACA AGGTCGGCGC GAAGGTGCCG ACCACCTGGC CCGAATTCTT CGCGGTGGCC GACAAGCTGA AGGCCGCGGG CATCCAGCCG GTCGCGATGG GCGGCCAGCC GTGGCAGGAC CTGACGCTGT GGGAAGACGT CGTGCTGTCG CAGGGCCCGG CGTTCTACAA GAAGGCGCTG GTCGACCTCG ACCAGGCGAC GCTGACGTCG CCGCAGATGC TGTCGGTGTT CGACACGGTG CGCAAGATCC AGGGCTACTT CGACACCGGC CGTAACGGCC GCGACTGGAA CCTCGCGACC GCGATGGTCA TCAACGGCAA GGCCGGCATG CAGTTCATGG GCGACTGGGC GAAGGGCGAA TTCGAAAACG CCGGCAAGAA GGCGGGCAAG GACTACGTCT GCGCGCCGGT GCCGGGCACC GCGAATTCGT ACACGTTCAA CGTCGATTCG TTCGTGTTCT TCCAGCAGAA GGGCGAGAAG AACGCGACGC CGGGCCAGCT CGCGCTCGCG AAGACGATCA TGACGCCGGA CTTCCAGGAG CAGTTCAGCC TGCTGAAGGG CTCGGTGCCG GTGCGTCTCG GCGTGAAGAT GGACAAGTTC GACGACTGCG CGAAGAAGTC GTATGCCGAC GAGCAGACCG CGATCAAGTC GGGCGGCTTC GTGCCGTCGC TCGCGCACGG CATGGCGCAA GGCGACGCGA CCGCCGGCGC GATCACCGAC GTCGTGACGA AGTTCATGAA CTCGCAGCAG GATTCGAAGA GCGCGGTCGC CGCGCTCGCG AAGGCCGCGA AGGTCAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT