SSGCID: Target Page

BuceA.18563.a

Putative type VI secretion system protein TssL

CENTER ID:	BuceA.18563.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18563.a.B1.GE37065	full length		1	260

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.354
RefSeq:	YP_002229503.1
UniProt:	B4E6Q9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYAPSLFGD NAPAPLHTPA STDSAFQARS LLDLLYDGFF MLFLLKNGRE PDSASEFSTR IQEFLSDFER GAKKLNIAAE DVYGAKFAFC AAIDEMVLSS QFKIRADWER RPLQLVLFGE QLAGEKFFQY LEEGRAQGAA RLQSLEVFHM CLLLGFQGKY LLEGPEKLAY LTARLGDEIA HMKGKRAPFA PHWPLPDQIA HRLKREVPVW AIGTVFALVA LLGYLGLNTY LKDKTLQALA PYSQVIKVGP ESANLTISLP

NT Sequence

atgagctacg cgccttcctt gttcggcgac aacgcgccgg caccgctgca taccccggcg tcgacggact ccgcgttcca ggcccgttcg ctgctcgatt tgctgtacga cgggttcttc atgctgttcc tgctcaagaa cggacgggag ccggacagcg ccagcgagtt cagcacgcgg atccaggaat tcctgtcgga tttcgagcgc ggcgcgaaga agctgaatat cgccgccgag gacgtgtacg gcgcgaagtt cgcgttctgc gcggccatcg acgagatggt gctgtcgtcg cagttcaaga tccgcgcgga ctgggaacgc cggccgctgc agctcgtgtt gttcggcgag cagctcgccg gcgagaagtt cttccagtat ctcgaggaag gtcgtgcgca gggtgcggca cggctgcagt cgctcgaggt gttccatatg tgcctgctgc tcgggttcca gggcaagtac ctgctcgaag ggccggaaaa gctcgcgtac ctgaccgcgc ggctgggcga cgagatcgcg cacatgaagg gcaagcgcgc gccgttcgcg ccgcactggc cgctgccgga ccagatcgcg caccgcttga agcgcgaggt gccggtgtgg gcgatcggga ccgtgttcgc gctggtggcg ctgctcgggt acctcgggct caacacgtac ctgaaggaca agacgctgca agcgctcgcg ccgtattcgc aggtgatcaa ggtcgggccg gagtcggcca acctgacgat ctcgttgccg taa

Details for BuceA.18563.a.B1.GE37065

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	30kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS YAPSLFGDNA PAPLHTPAST DSAFQARSLL DLLYDGFFML FLLKNGREPD SASEFSTRIQ EFLSDFERGA KKLNIAAEDV YGAKFAFCAA IDEMVLSSQF KIRADWERRP LQLVLFGEQL AGEKFFQYLE EGRAQGAARL QSLEVFHMCL LLGFQGKYLL EGPEKLAYLT ARLGDEIAHM KGKRAPFAPH WPLPDQIAHR LKREVPVWAI GTVFALVALL GYLGLNTYLK DKTLQALAPY SQVIKVGPES ANLTISLP

Validated NT Sequence

atcttactca cttacggcaa cgagatcgtc aggttggccg actccggccc gaccttgatc acctgcgaat acggcgcgag cgcttgcagc gtcttgtcct tcaggtacgt gttgagcccg aggtacccga gcagcgccac cagcgcgaac acggtcccga tcgcccacac cggcacctcg cgcttcaagc ggtgcgcgat ctggtccggc agcggccagt gcggcgcgaa cggcgcgcgc ttgcccttca tgtgcgcgat ctcgtcgccc agccgcgcgg tcaggtacgc gagcttttcc ggcccttcga gcaggtactt gccctggaac ccgagcagca ggcacatatg gaacacctcg agcgactgca gccgtgccgc accctgcgca cgaccttcct cgagatactg gaagaacttc tcgccggcga gctgctcgcc gaacaacacg agctgcagcg gccggcgttc ccagtccgcg cggatcttga actgcgacga cagcaccatc tcgtcgatgg ccgcgcagaa cgcgaacttc gcgccgtaca cgtcctcggc ggcgatattc agcttcttcg cgccgcgctc gaaatccgac aggaattcct ggatccgcgt gctgaactcg ctggcgctgt ccggctcccg tccgttcttg agcaggaaca gcatgaagaa cccgtcgtac agcaaatcga gcagcgaacg ggcctggaac gcggagtccg tcgacgccgg ggtatgcagc ggtgccggcg cgttgtcgcc gaacaaggaa ggcgcgtagc tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct acgcgccttc cttgttcggc gacaacgcgc cggcaccgct gcataccccg gcgtcgacgg actccgcgtt ccaggcccgt tcgctgctcg atttgctgta cgacgggttc ttcatgctgt tcctgctcaa gaacggacgg gagccggaca gcgccagcga gttcagcacg cggatccagg aattcctgtc ggatttcgag cgcggcgcga agaagctgaa tatcgccgcc gaggacgtgt acggcgcgaa gttcgcgttc tgcgcggcca tcgacgagat ggtgctgtcg tcgcagttca agatccgcgc ggactgggaa cgccggccgc tgcagctcgt gttgttcggc gagcagctcg ccggcgagaa gttcttccag tatctcgagg aaggtcgtgc gcagggtgcg gcacggctgc agtcgctcga ggtgttccat atgtgcctgc tgctcgggtt ccagggcaag tacctgctcg aagggccgga aaagctcgcg tacctgaccg cgcggctggg cgacgagatc gcgcacatga agggcaagcg cgcgccgttc gcgccgcact ggccgctgcc ggaccagatc gcgcaccgct tgaagcgcga ggtgccggtg tgggcgatcg ggaccgtgtt cgcgctggtg gcgctgctcg ggtacctcgg gctcaacacg tacctgaagg acaagacgct gcaagcgctc gcgccgtatt cgcaggtgat caaggtcggg ccggagtcgg ccaacctgac gatctcgttg ccgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat