SSGCID: Target Page

BuceA.18559.b

Putative exported protein

CENTER ID:	BuceA.18559.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18559.b.B1.GE37063	full length		1	193

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3433
RefSeq:	YP_002232413.1
UniProt:	B4EE31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKHLIIAAG ALAASLSFSA FAESATYQFD PSHTYPSFEA DHFGGLSVWR GKFDKSSGTV TLDRAAKTGT VDVTTDIASI HTGSAKLDEH LQTAEFFDAA KFPQANYKGT IKFDGDKPVS VVGNLTLHGV TKPLTLKIDS FKCMPHPMLK REVCGVDAVG EFSRDDFGLD YGKQYGFKMK TKLLITAEAV KQQ

NT Sequence

ttgaaaaagc atctgatcat cgccgccggc gcgctggccg cttcgctgtc gttctcggct ttcgccgaaa gcgccaccta ccagttcgac ccgagccaca cgtacccgag cttcgaggcc gaccacttcg gcggcctgtc ggtctggcgc ggcaagttcg acaagtcgag cggcaccgtg acgctcgacc gtgcggcgaa gaccggcacg gtcgacgtga cgaccgacat cgcgtcgatc cacacgggca gcgcgaagct cgacgagcac ctgcagacgg ccgagttctt cgatgcggcg aagttcccgc aggcgaacta caagggcacg atcaagttcg acggcgacaa gccggtgtcg gtggtcggca acctgacgct gcatggcgtc acgaagccgc tcacgctgaa gatcgattcg ttcaagtgca tgccgcaccc gatgctcaag cgtgaagtgt gcggcgtcga cgcggtcggc gaattcagcc gcgacgattt cggcctcgac tacggcaagc agtacggctt caagatgaag acgaagctgc tgatcacggc cgaagcggtc aagcagcagt aa

Details for BuceA.18559.b.B1.GE37063

HARVESTED ON:	6/12/2013
SEQUENCED ON:	6/11/2013
EXPECTED MW:	22kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KKHLIIAAGA LAASLSFSAF AESATYQFDP SHTYPSFEAD HFGGLSVWRG KFDKSSGTVT LDRAAKTGTV DVTTDIASIH TGSAKLDEHL QTAEFFDAAK FPQANYKGTI KFDGDKPVSV VGNLTLHGVT KPLTLKIDSF KCMPHPMLKR EVCGVDAVGE FSRDDFGLDY GKQYGFKMKT KLLITAEAVK QQ

Validated NT Sequence

ttttnnttan ctttaagaag gaganatacc atggctcacc accaccacca ccatatgttg aaaaagcatc tgatcatcgc cgccggcgcg ctggccgctt cgctgtcgtt ctcggctttc gccgaaagcg ccacctacca gttcgacccg agccacacgt acccgagctt cgaggccgac cacttcggcg gcctgtcggt ctggcgcggc aagttcgaca agtcgagcgg caccgtgacg ctcgaccgtg cggcgaagac cggcacggtc gacgtgacga ccgacatcgc gtcgatccac acgggcagcg cgaagctcga cgagcacctg cagacggccg agttcttcga tgcggcgaag ttcccgcagg cgaactacaa gggcacgatc aagttcgacg gcgacaagcc ggtgtcggtg gtcggcaacc tgacgctgca tggcgtcacg aagccgctca cgctgaagat cgattcgttc aagtgcatgc cgcacccgat gctcaagcgt gaagtgtgcg gcgtcgacgc ggtcggcgaa ttcagccgcg acgatttcgg cctcgactac ggcaagcagt acggcttcaa gatgaagacg aagctgctga tcacggccga agcggtcaag cagcagtaag tgagtaagat aggatccggc tgctaacaag ccccgaaaga nnnnnnnnnn annnntgann nnnnnnnnnc nnnnnnnnna ncnncnnanc nccnnnnnnc

Expected Protein Sequence

MAHHHHHHMK KHLIIAAGAL AASLSFSAFA ESATYQFDPS HTYPSFEADH FGGLSVWRGK FDKSSGTVTL DRAAKTGTVD VTTDIASIHT GSAKLDEHLQ TAEFFDAAKF PQANYKGTIK FDGDKPVSVV GNLTLHGVTK PLTLKIDSFK CMPHPMLKRE VCGVDAVGEF SRDDFGLDYG KQYGFKMKTK LLITAEAVKQ Q

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaaa agcatctgat catcgccgcc ggcgcgctgg ccgcttcgct gtcgttctcg gctttcgccg aaagcgccac ctaccagttc gacccgagcc acacgtaccc gagcttcgag gccgaccact tcggcggcct gtcggtctgg cgcggcaagt tcgacaagtc gagcggcacc gtgacgctcg accgtgcggc gaagaccggc acggtcgacg tgacgaccga catcgcgtcg atccacacgg gcagcgcgaa gctcgacgag cacctgcaga cggccgagtt cttcgatgcg gcgaagttcc cgcaggcgaa ctacaagggc acgatcaagt tcgacggcga caagccggtg tcggtggtcg gcaacctgac gctgcatggc gtcacgaagc cgctcacgct gaagatcgat tcgttcaagt gcatgccgca cccgatgctc aagcgtgaag tgtgcggcgt cgacgcggtc ggcgaattca gccgcgacga tttcggcctc gactacggca agcagtacgg cttcaagatg aagacgaagc tgctgatcac ggccgaagcg gtcaagcagc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat