SSGCID: Target Page

BuceA.18479.a

DNA repair protein recO (Recombination protein O)

CENTER ID:	BuceA.18479.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18479.a.B1.GE35014	full length		1	278

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18479.a.B1.PS01524	full length		1	278

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2967
RefSeq:	YP_002230161.1
UniProt:	B4EA46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGTNDALTST EDAVAAGAND APLPAPPEPP RKARRATSRT SDFRVAEQPA FVLHSYPYRE TSLIVDVLTR DHGRLALVAK GAKRPHSALR GVLQTFQPLL LSWSGKSEVR TLTGAEWVGG MLPLGGDGLL CGFYANELLV KFCAREDPQP PLFNHYVLTL TRLAHGEPAV QVLRSFERVL LRETGYAMAL NRTVARRAVE PERRYVFDPE RGVRNADDDV PSHWPVITGQ TLLDMEQDDY HRAQTVAQSK TLMRFLLNTY LGGTPLATRQ ILIDLQNL

NT Sequence

atgggtacga atgacgcgct gacgtcgacc gaagacgcgg tggcggccgg cgcgaacgac gcgccgttgc cggcaccgcc cgaaccgccg cgcaaggcgc ggcgcgcgac gtctcgcacg tccgatttcc gcgtcgccga gcagccggcg ttcgtgctgc acagctatcc gtatcgggaa acgagcctga tcgtcgacgt gctgacacgt gaccacggcc ggctcgcgct cgtcgcgaaa ggcgcgaagc gtccgcactc cgcgttgcgc ggcgtgttgc agacgttcca gccgctgctg ctgtcctggt ccggcaaatc cgaggtgcgc acgctgacgg gcgccgagtg ggtcggcggg atgctgccgc tcggcggcga cggcctgttg tgcggtttct acgcgaacga gctgctcgtg aaattctgcg cccgcgagga tccgcagccg ccgctgttca atcattacgt gctgaccctc acgcgtctcg cgcacggcga gcccgcggtg caggtgctgc gctcgttcga acgcgtgctg ctgcgcgaga ccggctatgc gatggcgctg aaccgcacgg tcgcccgtcg cgcggtcgaa cccgagcgcc gctacgtgtt cgatccggag cgtggcgtac gcaatgcgga cgacgacgtg ccgtcgcact ggccggtcat caccggccag acgttgctcg acatggagca ggacgattac catcgagccc agacggttgc gcagagcaag acgctgatgc gcttcctgct gaacacctat ctcggcggta cgccgctcgc cacgcgtcag atcctgatcg acctgcaaaa cctatga

Details for BuceA.18479.a.B1.GE35014

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	20
PERCENT COVERAGE:	83

Validated AA Sequence

MVIVLLHVEQ RLAGDDRPVR RHVVVRIAYA TLRIEHVAAL GFDRATGDRA VQRHRIAGLA QQHAFERAQH LHRGLAVRET REGQHVMIEQ RRLRILAGAE FHEQLVRVET AQQAVAAERQ HPADPLGARQ RAHLGFAGPG QQQRLERLQH AAQRGVRTLR AFRDEREPAV VTCQHVDDQA RFPIRIAVQH ERRLLGDAEI GRARRRAPRL ARRFGRCRQR RVVRAGRHRV FGRRQRVI

Validated NT Sequence

tactcactta taggttttgc aggtcgatca ggatctgacg cgtggcgagc ggcgtaccgc cgagataggt gttcagcagg aagcgcatca gcgtcttgct ctgcgcaacc gtctgggctc gatggtaatc gtcctgctcc atgtcgagca acgtctggcc ggtgatgacc ggccagtgcg acggcacgtc gtcgtccgca ttgcgtacgc cacgctccgg atcgaacacg tagcggcgct cgggttcgac cgcgcgacgg gcgaccgtgc ggttcagcgc catcgcatag ccggtctcgc gcagcagcac gcgttcgaac gagcgcagca cctgcaccgc gggctcgccg tgcgcgagac gcgtgagggt cagcacgtaa tgattgaaca gcggcggctg cggatcctcg cgggcgcaga atttcacgag cagctcgttc gcgtagaaac cgcacaacag gccgtcgccg ccgagcggca gcatcccgcc gacccactcg gcgcccgtca gcgtgcgcac ctcggatttg ccggaccagg acagcagcag cggctggaac gtctgcaaca cgccgcgcaa cgcggagtgc ggacgcttcg cgcctttcgc gacgagcgcg agccggccgt ggtcacgtgt cagcacgtcg acgatcaggc tcgtttcccg atacggatag ctgtgcagca cgaacgccgg ctgctcggcg acgcggaaat cggacgtgcg agacgtcgcg cgccgcgcct tgcgcggcgg ttcgggcggt gccggcaacg gcgcgtcgtt cgcgccggcc gccaccgcgt cttcggtcga cgtcagcgcg tcattcg

Expected Protein Sequence

MAHHHHHHMG TNDALTSTED AVAAGANDAP LPAPPEPPRK ARRATSRTSD FRVAEQPAFV LHSYPYRETS LIVDVLTRDH GRLALVAKGA KRPHSALRGV LQTFQPLLLS WSGKSEVRTL TGAEWVGGML PLGGDGLLCG FYANELLVKF CAREDPQPPL FNHYVLTLTR LAHGEPAVQV LRSFERVLLR ETGYAMALNR TVARRAVEPE RRYVFDPERG VRNADDDVPS HWPVITGQTL LDMEQDDYHR AQTVAQSKTL MRFLLNTYLG GTPLATRQIL IDLQNL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggta cgaatgacgc gctgacgtcg accgaagacg cggtggcggc cggcgcgaac gacgcgccgt tgccggcacc gcccgaaccg ccgcgcaagg cgcggcgcgc gacgtctcgc acgtccgatt tccgcgtcgc cgagcagccg gcgttcgtgc tgcacagcta tccgtatcgg gaaacgagcc tgatcgtcga cgtgctgaca cgtgaccacg gccggctcgc gctcgtcgcg aaaggcgcga agcgtccgca ctccgcgttg cgcggcgtgt tgcagacgtt ccagccgctg ctgctgtcct ggtccggcaa atccgaggtg cgcacgctga cgggcgccga gtgggtcggc gggatgctgc cgctcggcgg cgacggcctg ttgtgcggtt tctacgcgaa cgagctgctc gtgaaattct gcgcccgcga ggatccgcag ccgccgctgt tcaatcatta cgtgctgacc ctcacgcgtc tcgcgcacgg cgagcccgcg gtgcaggtgc tgcgctcgtt cgaacgcgtg ctgctgcgcg agaccggcta tgcgatggcg ctgaaccgca cggtcgcccg tcgcgcggtc gaacccgagc gccgctacgt gttcgatccg gagcgtggcg tacgcaatgc ggacgacgac gtgccgtcgc actggccggt catcaccggc cagacgttgc tcgacatgga gcaggacgat taccatcgag cccagacggt tgcgcagagc aagacgctga tgcgcttcct gctgaacacc tatctcggcg gtacgccgct cgccacgcgt cagatcctga tcgacctgca aaacctatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BuceA.18479.a.B1.PS01524

PURIFICATION DATe:	6/6/2012
CONCENTRATION:	7.83mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	20
PERCENT COVERAGE:	83

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTA CGAATGACGC GCTGACGTCG ACCGAAGACG CGGTGGCGGC CGGCGCGAAC GACGCGCCGT TGCCGGCACC GCCCGAACCG CCGCGCAAGG CGCGGCGCGC GACGTCTCGC ACGTCCGATT TCCGCGTCGC CGAGCAGCCG GCGTTCGTGC TGCACAGCTA TCCGTATCGG GAAACGAGCC TGATCGTCGA CGTGCTGACA CGTGACCACG GCCGGCTCGC GCTCGTCGCG AAAGGCGCGA AGCGTCCGCA CTCCGCGTTG CGCGGCGTGT TGCAGACGTT CCAGCCGCTG CTGCTGTCCT GGTCCGGCAA ATCCGAGGTG CGCACGCTGA CGGGCGCCGA GTGGGTCGGC GGGATGCTGC CGCTCGGCGG CGACGGCCTG TTGTGCGGTT TCTACGCGAA CGAGCTGCTC GTGAAATTCT GCGCCCGCGA GGATCCGCAG CCGCCGCTGT TCAATCATTA CGTGCTGACC CTCACGCGTC TCGCGCACGG CGAGCCCGCG GTGCAGGTGC TGCGCTCGTT CGAACGCGTG CTGCTGCGCG AGACCGGCTA TGCGATGGCG CTGAACCGCA CGGTCGCCCG TCGCGCGGTC GAACCCGAGC GCCGCTACGT GTTCGATCCG GAGCGTGGCG TACGCAATGC GGACGACGAC GTGCCGTCGC ACTGGCCGGT CATCACCGGC CAGACGTTGC TCGACATGGA GCAGGACGAT TACCATCGAG CCCAGACGGT TGCGCAGAGC AAGACGCTGA TGCGCTTCCT GCTGAACACC TATCTCGGCG GTACGCCGCT CGCCACGCGT CAGATCCTGA TCGACCTGCA AAACCTATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT