SSGCID: Target Page

BuceA.18475.a

Putative exported protein

CENTER ID:	BuceA.18475.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18475.a.B2.GE42113	PF14334_0 DUF		33	182

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.490
RefSeq:	YP_002229632.1
UniProt:	B4E7W2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTIKHLFPFR LAAVLLVALT LCLAVVRPAH AESIAVQRAS LQSDGSGWSL DARFDFELNP NLEDAVNKGI PLYFTTDFEL SRARWYWFDE QPVAVTQTIR LSFQPLTREY RVSTGGLQLG FPSLKDALAV VRHITSWHVI DRNQVRAGET YTASVRMQLD TALMPKPFQV DAVNNRDWTL GSDWKRFNFT VTERAK

NT Sequence

gtgacgatca aacacctttt tccctttcgg ctcgcggccg tcttgctggt cgcgttgacg ctgtgcttgg ccgtcgtgcg gccggcgcat gccgaatcga tcgccgtgca acgcgcgtcg ctccagtccg acggaagcgg ctggagcctc gacgcacggt tcgacttcga gctgaatccg aacctcgagg atgccgtcaa caagggcatc ccgctttact tcacgaccga cttcgagctg agccgcgcgc gctggtactg gttcgacgag cagccggtgg cggtcacgca gacgatccgc ctgtcgttcc agccgctcac gcgcgagtac cgcgtgtcga cgggcggcct gcagctcggc ttcccgtcgc tgaaggatgc gctcgcggtg gtccggcaca tcacgtcgtg gcacgtgatc gaccgtaacc aggtgcgtgc cggcgaaacc tacacggcgt cggtgcggat gcagctcgat acggcgctga tgccgaagcc gttccaggtc gacgcggtga acaatcgcga ctggacgctc gggtcggact ggaagcgctt caacttcacg gtgaccgaac gtgctaaata a

Details for BuceA.18475.a.B2.GE42113

HARVESTED ON:	1/16/2018
SEQUENCED ON:	1/17/2018
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMS IAVQRASLQS DGSGWSLDAR FDFELNPNLE DAVNKGIPLY FTTDFELSRA RWYWFDEQPV AVTQTIRLSF QPLTREYRVS TGGLQLGFPS LKDALAVVRH ITSWHVIDRN QVRAGETYTA SVRMQLDTAL MPKPFQVDAV NNRDWTLGS

Validated NT Sequence

atggctcacc accaccacca ccatatgtcg atcgccgtgc aacgcgcgtc gctccagtcc gacggaagcg gctggagcct cgacgcacgg ttcgacttcg agctgaatcc gaacctcgag gatgccgtca acaagggcat cccgctttac ttcacgaccg acttcgagct gagccgcgcg cgctggtact ggttcgacga gcagccggtg gcggtcacgc agacgatccg cctgtcgttc cagccgctca cgcgcgagta ccgcgtgtcg acgggcggcc tgcagctcgg cttcccgtcg ctgaaggatg cgctcgcggt ggtccggcac atcacgtcgt ggcacgtgat cgaccgtaac caggtgcgtg ccggcgaaac ctacacggcg tcggtgcgga tgcagctcga tacggcgctg atgccgaagc cgttccaggt cgacgcggtg aacaatcgcg actggacgct cgggtcgtaa gtgagtaaga t

Expected Protein Sequence

MAHHHHHHSI AVQRASLQSD GSGWSLDARF DFELNPNLED AVNKGIPLYF TTDFELSRAR WYWFDEQPVA VTQTIRLSFQ PLTREYRVST GGLQLGFPSL KDALAVVRHI TSWHVIDRNQ VRAGETYTAS VRMQLDTALM PKPFQVDAVN NRDWTLGS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattcgatcg ccgtgcaacg cgcgtcgctc cagtccgacg gaagcggctg gagcctcgac gcacggttcg acttcgagct gaatccgaac ctcgaggatg ccgtcaacaa gggcatcccg ctttacttca cgaccgactt cgagctgagc cgcgcgcgct ggtactggtt cgacgagcag ccggtggcgg tcacgcagac gatccgcctg tcgttccagc cgctcacgcg cgagtaccgc gtgtcgacgg gcggcctgca gctcggcttc ccgtcgctga aggatgcgct cgcggtggtc cggcacatca cgtcgtggca cgtgatcgac cgtaaccagg tgcgtgccgg cgaaacctac acggcgtcgg tgcggatgca gctcgatacg gcgctgatgc cgaagccgtt ccaggtcgac gcggtgaaca atcgcgactg gacgctcggg tcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat