BuceA.18460.a

Isocitrate dehydrogenase [NADP] (EC 1.1.1.42)

CENTER ID: BuceA.18460.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18460.a.B1.GE35045 full length 1 745
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.18460.a.B1.PW35660 full length 1 745

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2831
RefSeq: YP_002231838.1
UniProt: B4E908

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTMSTSPKI IYTLTDEAPA LATYSLLPIV KAFTRSSGVA VETRDISLAG RIIAAFADVL PSEQKGSDDL AELGQLTLKP EANIIKLPNI SASVPQLKAA IAELQAQGYA LPAYPEDPST DEEKAVKARY DKIKGSAVNP VLREGNSDRR APLSVKNYAR KHPHKMGAWK STSKAHVAHM SEGDFYGSEK SALIAEAGSV KIELTTTDGA KKVLKEKTAV KAGEVIDASV MSRNALRSFI EAQIADAKAQ DVLFSVHLKA TMMKVSDPIL FGHFVSVFYR DALTKHADVL AKAGFNPNNG IGDLYARLKD LPADTREAIE ADVKAEYAVR PRLAMVNSDK GITNLHVPSD VIVDASMPAM IRDSGGMWGP DGQLYDAKAV IPDRCYAGVY QAVIEDCKQH GAFDPVTMGS VPNVGLMAQA AEEYGSHDKT FQIPADGVVR VTDEAGNVLL EHAVEAGDIW RMCQTKDAPV QDWVKLAVNR ARATGAPAVF WLDPARAHDA QIIAKVERYL KDHDTNGLDI RIMTPVDATR FSLERIRAGK DTISVTGNVL RDYLTDLFPI MELGTSAKML SIVPLMAGGG MFETGAGGSA PKHVQQFVEE GFLRWDSLGE FLALAASLEH LGNAYQNPKA LVLAKTLDQA TGKFLDENKS PARKVGGLDN RGSHFYLCLY WAQALAEQTE DAALKAQFED VAKALSDNEA RILEELAAAQ GKPQAIGGYY RPNVDLTSQA MRPSATLNGI VDAVA
NT Sequence
ttgaccacca tgtccacttc gcccaagatc atctacaccc tcaccgacga agcgccggcg ctcgcgacct attcgctgct gccgatcgtc aaggccttca cgcgttcgtc cggcgtcgcc gtcgaaacgc gcgacatctc gctcgccggc cgcatcatcg cggcattcgc agacgtcctg ccgtcggagc agaagggttc cgacgatctg gccgagctgg gccagctcac gctgaagccg gaagcgaaca tcatcaagct gccgaacatc agcgcatcgg tgccgcagct gaaggccgcg atcgccgaac tgcaggcgca gggctacgcg ctgccggcct atccggaaga tccgtcgacg gacgaagaga aggccgtcaa ggcgcgctac gacaagatca agggcagcgc ggtgaacccg gtgctgcgcg aaggcaactc cgaccgccgc gcgccgctgt cggtcaagaa ctacgcacgc aagcatccgc acaagatggg cgcgtggaag tccacgtcga aggcccacgt cgcgcacatg agcgaaggcg acttctacgg cagcgagaag tcggcgctga tcgccgaggc cggcagcgtg aagatcgaac tcacgacgac cgacggcgcg aagaaggtgc tgaaggaaaa gacggccgtg aaggccggtg aagtgatcga cgcatcggtg atgagccgca acgcgctgcg cagcttcatc gaagcgcaga tcgcggacgc gaaggcgcag gacgtgctgt tctcggtgca cctgaaggcg accatgatga aggtctcgga cccgatcctg ttcggtcact tcgtgtcggt gttctaccgc gacgcgctca cgaagcacgc ggacgtgctg gcgaaggccg gcttcaatcc gaacaacggg attggcgacc tgtacgcgcg cctgaaggac ctgccggccg acacgcgcga agcgatcgag gccgacgtca aggccgagta cgcggtgcgc ccgcgcctcg cgatggtcaa ctcggacaag ggcatcacga acctgcacgt gccgagcgac gtgatcgtcg acgcatcgat gccggcgatg atccgcgatt cgggcggcat gtggggcccg gacggtcaac tgtacgacgc gaaggccgtg attccggacc gctgctacgc aggcgtgtac caggccgtga tcgaggactg caagcagcac ggcgcgttcg atccggtcac gatgggcagc gtgccgaacg taggcctgat ggcgcaggcg gccgaagaat acggttcgca cgacaagacg ttccagatcc cggcggacgg cgtcgtgcgc gtcaccgacg aagccggcaa cgtgctgctc gagcacgcgg tcgaggcggg cgacatctgg cgcatgtgcc agacgaagga cgcaccggtg caggactggg tcaagctcgc ggtcaaccgc gcccgcgcga ccggcgcccc ggccgtgttc tggctcgatc cggcgcgcgc gcacgatgcg cagatcatcg cgaaggtcga acgctacctg aaggatcacg acacgaacgg cctcgacatc cggatcatga cgccggtcga cgccacgcgt ttctcgctcg agcgcatccg cgcgggcaag gacacgattt cggtcaccgg caacgtgctg cgcgattacc tgaccgatct gttcccgatc atggaactcg gcaccagcgc gaagatgctg tcgatcgtgc cgctgatggc cggcggcggg atgttcgaaa cgggggcggg cggctccgcg ccgaagcacg tgcagcagtt cgtcgaggaa ggcttcctgc gctgggattc gctcggcgaa ttcctcgcgc tggccgcgtc gctcgagcac ctcggcaacg cgtaccagaa cccgaaggcg ctcgtgctcg cgaagacgct cgaccaggca accggcaagt tcctggacga gaacaagtcg ccggcgcgca aggtcggcgg cctcgacaac cgcggcagcc acttctacct gtgcctgtac tgggcgcagg cattggccga gcagaccgag gacgccgcgc tgaaggcgca gttcgaggac gtggcgaagg cgctgtccga caacgaagcg cgcatcctgg aggaactggc ggccgcgcag ggcaagccgc aggcgatcgg cggctactac cgtccgaacg tcgacctgac gagccaggcc atgcgcccga gcgcgacgct gaacggcatc gtcgacgcgg tcgcctga
Details for BuceA.18460.a.B1.GE35045
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 81kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 35
Validated AA Sequence
MAHHHHHHML TTMSTSPKII YTLTDEAPAL ATYSLLPIVK AFTRSSGVAV ETRDISLAGR IIAAFADVLP SEQKGSDDLA ELGQLTLKPE ANIIKLPNIS ASVPQLKAAI AELQAQGYAL PAYPEDPSTD EEKAVKARYD KIKGSAVNPV LREGNSDRRA PLSVKNYARK HPHKMGAWKS TSKAHVAHMS EGDFYGSEKS ALIAEAGSVK IELTTTDGAK XVLKEKTAVK AGEVIDASVM SRNALRSFIE AQIADAKAXX CCSRCT
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg accaccatgt ccacttcgcc caagatcatc tacaccctca ccgacgaagc gccggcgctc gcgacctatt cgctgctgcc gatcgtcaag gccttcacgc gttcgtccgg cgtcgccgtc gaaacgcgcg acatctcgct cgccggccgc atcatcgcgg cattcgcaga cgtcctgccg tcggagcaga agggttccga cgatctggcc gagctgggcc agctcacgct gaagccggaa gcgaacatca tcaagctgcc gaacatcagc gcatcggtgc cgcagctgaa ggccgcgatc gccgaactgc aggcgcaggg ctacgcgctg ccggcctatc cggaagatcc gtcgacggac gaagagaagg ccgtcaaggc gcgctacgac aagatcaagg gcagcgcggt gaacccggtg ctgcgcgaag gcaactccga ccgccgcgcg ccgctgtcgg tcaagaacta cgcacgcaag catccgcaca agatgggcgc gtggaagtcc acgtcgaagg cccacgtcgc gcacatgagc gaaggcgact tctacggcag cgagaagtcg gcgctgatcg ccgaggccgg cagcgtgaag atcgaactca cgacgaccga cggcgcgaag aangtgctga aggaaaagac ggccgtgaag gccggtgaag tgatcgacgc atcggtgatg agccgcaacg cgctgcgcag cttcatcgaa gcgcagatcg cggacgcgaa ggcgcnnncg tgctgttctc ggtgcacctg aaggcgacca tgatgannnc tcgganccga tcctgttcgg tcacttcgtg tcggtgttct accgcgacgc gctcacgaag cacgcngacg tgctggcgac ggcttnnncc nannnnggan tgnnganntg nacgcgcncc tgannnctcn nnnannnnnn nnnannnatc nnngnnnnnn nnngnnnnnn nnncgnnncn nnnnnnnnnn nnnnngnnan nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnt
Expected Protein Sequence
MAHHHHHHML TTMSTSPKII YTLTDEAPAL ATYSLLPIVK AFTRSSGVAV ETRDISLAGR IIAAFADVLP SEQKGSDDLA ELGQLTLKPE ANIIKLPNIS ASVPQLKAAI AELQAQGYAL PAYPEDPSTD EEKAVKARYD KIKGSAVNPV LREGNSDRRA PLSVKNYARK HPHKMGAWKS TSKAHVAHMS EGDFYGSEKS ALIAEAGSVK IELTTTDGAK KVLKEKTAVK AGEVIDASVM SRNALRSFIE AQIADAKAQD VLFSVHLKAT MMKVSDPILF GHFVSVFYRD ALTKHADVLA KAGFNPNNGI GDLYARLKDL PADTREAIEA DVKAEYAVRP RLAMVNSDKG ITNLHVPSDV IVDASMPAMI RDSGGMWGPD GQLYDAKAVI PDRCYAGVYQ AVIEDCKQHG AFDPVTMGSV PNVGLMAQAA EEYGSHDKTF QIPADGVVRV TDEAGNVLLE HAVEAGDIWR MCQTKDAPVQ DWVKLAVNRA RATGAPAVFW LDPARAHDAQ IIAKVERYLK DHDTNGLDIR IMTPVDATRF SLERIRAGKD TISVTGNVLR DYLTDLFPIM ELGTSAKMLS IVPLMAGGGM FETGAGGSAP KHVQQFVEEG FLRWDSLGEF LALAASLEHL GNAYQNPKAL VLAKTLDQAT GKFLDENKSP ARKVGGLDNR GSHFYLCLYW AQALAEQTED AALKAQFEDV AKALSDNEAR ILEELAAAQG KPQAIGGYYR PNVDLTSQAM RPSATLNGIV DAVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga ccaccatgtc cacttcgccc aagatcatct acaccctcac cgacgaagcg ccggcgctcg cgacctattc gctgctgccg atcgtcaagg ccttcacgcg ttcgtccggc gtcgccgtcg aaacgcgcga catctcgctc gccggccgca tcatcgcggc attcgcagac gtcctgccgt cggagcagaa gggttccgac gatctggccg agctgggcca gctcacgctg aagccggaag cgaacatcat caagctgccg aacatcagcg catcggtgcc gcagctgaag gccgcgatcg ccgaactgca ggcgcagggc tacgcgctgc cggcctatcc ggaagatccg tcgacggacg aagagaaggc cgtcaaggcg cgctacgaca agatcaaggg cagcgcggtg aacccggtgc tgcgcgaagg caactccgac cgccgcgcgc cgctgtcggt caagaactac gcacgcaagc atccgcacaa gatgggcgcg tggaagtcca cgtcgaaggc ccacgtcgcg cacatgagcg aaggcgactt ctacggcagc gagaagtcgg cgctgatcgc cgaggccggc agcgtgaaga tcgaactcac gacgaccgac ggcgcgaaga aggtgctgaa ggaaaagacg gccgtgaagg ccggtgaagt gatcgacgca tcggtgatga gccgcaacgc gctgcgcagc ttcatcgaag cgcagatcgc ggacgcgaag gcgcaggacg tgctgttctc ggtgcacctg aaggcgacca tgatgaaggt ctcggacccg atcctgttcg gtcacttcgt gtcggtgttc taccgcgacg cgctcacgaa gcacgcggac gtgctggcga aggccggctt caatccgaac aacgggattg gcgacctgta cgcgcgcctg aaggacctgc cggccgacac gcgcgaagcg atcgaggccg acgtcaaggc cgagtacgcg gtgcgcccgc gcctcgcgat ggtcaactcg gacaagggca tcacgaacct gcacgtgccg agcgacgtga tcgtcgacgc atcgatgccg gcgatgatcc gcgattcggg cggcatgtgg ggcccggacg gtcaactgta cgacgcgaag gccgtgattc cggaccgctg ctacgcaggc gtgtaccagg ccgtgatcga ggactgcaag cagcacggcg cgttcgatcc ggtcacgatg ggcagcgtgc cgaacgtagg cctgatggcg caggcggccg aagaatacgg ttcgcacgac aagacgttcc agatcccggc ggacggcgtc gtgcgcgtca ccgacgaagc cggcaacgtg ctgctcgagc acgcggtcga ggcgggcgac atctggcgca tgtgccagac gaaggacgca ccggtgcagg actgggtcaa gctcgcggtc aaccgcgccc gcgcgaccgg cgccccggcc gtgttctggc tcgatccggc gcgcgcgcac gatgcgcaga tcatcgcgaa ggtcgaacgc tacctgaagg atcacgacac gaacggcctc gacatccgga tcatgacgcc ggtcgacgcc acgcgtttct cgctcgagcg catccgcgcg ggcaaggaca cgatttcggt caccggcaac gtgctgcgcg attacctgac cgatctgttc ccgatcatgg aactcggcac cagcgcgaag atgctgtcga tcgtgccgct gatggccggc ggcgggatgt tcgaaacggg ggcgggcggc tccgcgccga agcacgtgca gcagttcgtc gaggaaggct tcctgcgctg ggattcgctc ggcgaattcc tcgcgctggc cgcgtcgctc gagcacctcg gcaacgcgta ccagaacccg aaggcgctcg tgctcgcgaa gacgctcgac caggcaaccg gcaagttcct ggacgagaac aagtcgccgg cgcgcaaggt cggcggcctc gacaaccgcg gcagccactt ctacctgtgc ctgtactggg cgcaggcatt ggccgagcag accgaggacg ccgcgctgaa ggcgcagttc gaggacgtgg cgaaggcgct gtccgacaac gaagcgcgca tcctggagga actggcggcc gcgcagggca agccgcaggc gatcggcggc tactaccgtc cgaacgtcga cctgacgagc caggccatgc gcccgagcgc gacgctgaac ggcatcgtcg acgcggtcgc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuceA.18460.a.B1.PW35660
PURIFICATION DATe: 4/24/2012
CONCENTRATION: 37.12mg/ml
OBSERVED MW: 80kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 35
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML TTMSTSPKII YTLTDEAPAL ATYSLLPIVK AFTRSSGVAV ETRDISLAGR IIAAFADVLP SEQKGSDDLA ELGQLTLKPE ANIIKLPNIS ASVPQLKAAI AELQAQGYAL PAYPEDPSTD EEKAVKARYD KIKGSAVNPV LREGNSDRRA PLSVKNYARK HPHKMGAWKS TSKAHVAHMS EGDFYGSEKS ALIAEAGSVK IELTTTDGAK XVLKEKTAVK AGEVIDASVM SRNALRSFIE AQIADAKAXX CCSRCT
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcacc accaccacca ccatatgttg accaccatgt ccacttcgcc caagatcatc tacaccctca ccgacgaagc gccggcgctc gcgacctatt cgctgctgcc gatcgtcaag gccttcacgc gttcgtccgg cgtcgccgtc gaaacgcgcg acatctcgct cgccggccgc atcatcgcgg cattcgcaga cgtcctgccg tcggagcaga agggttccga cgatctggcc gagctgggcc agctcacgct gaagccggaa gcgaacatca tcaagctgcc gaacatcagc gcatcggtgc cgcagctgaa ggccgcgatc gccgaactgc aggcgcaggg ctacgcgctg ccggcctatc cggaagatcc gtcgacggac gaagagaagg ccgtcaaggc gcgctacgac aagatcaagg gcagcgcggt gaacccggtg ctgcgcgaag gcaactccga ccgccgcgcg ccgctgtcgg tcaagaacta cgcacgcaag catccgcaca agatgggcgc gtggaagtcc acgtcgaagg cccacgtcgc gcacatgagc gaaggcgact tctacggcag cgagaagtcg gcgctgatcg ccgaggccgg cagcgtgaag atcgaactca cgacgaccga cggcgcgaag aangtgctga aggaaaagac ggccgtgaag gccggtgaag tgatcgacgc atcggtgatg agccgcaacg cgctgcgcag cttcatcgaa gcgcagatcg cggacgcgaa ggcgcnnncg tgctgttctc ggtgcacctg aaggcgacca tgatgannnc tcgganccga tcctgttcgg tcacttcgtg tcggtgttct accgcgacgc gctcacgaag cacgcngacg tgctggcgac ggcttnnncc nannnnggan tgnnganntg nacgcgcncc tgannnctcn nnnannnnnn nnnannnatc nnngnnnnnn nnngnnnnnn nnncgnnncn nnnnnnnnnn nnnnngnnan nnnnnnngnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnt
Expressed Protein Sequence
MAHHHHHHML TTMSTSPKII YTLTDEAPAL ATYSLLPIVK AFTRSSGVAV ETRDISLAGR IIAAFADVLP SEQKGSDDLA ELGQLTLKPE ANIIKLPNIS ASVPQLKAAI AELQAQGYAL PAYPEDPSTD EEKAVKARYD KIKGSAVNPV LREGNSDRRA PLSVKNYARK HPHKMGAWKS TSKAHVAHMS EGDFYGSEKS ALIAEAGSVK IELTTTDGAK KVLKEKTAVK AGEVIDASVM SRNALRSFIE AQIADAKAQD VLFSVHLKAT MMKVSDPILF GHFVSVFYRD ALTKHADVLA KAGFNPNNGI GDLYARLKDL PADTREAIEA DVKAEYAVRP RLAMVNSDKG ITNLHVPSDV IVDASMPAMI RDSGGMWGPD GQLYDAKAVI PDRCYAGVYQ AVIEDCKQHG AFDPVTMGSV PNVGLMAQAA EEYGSHDKTF QIPADGVVRV TDEAGNVLLE HAVEAGDIWR MCQTKDAPVQ DWVKLAVNRA RATGAPAVFW LDPARAHDAQ IIAKVERYLK DHDTNGLDIR IMTPVDATRF SLERIRAGKD TISVTGNVLR DYLTDLFPIM ELGTSAKMLS IVPLMAGGGM FETGAGGSAP KHVQQFVEEG FLRWDSLGEF LALAASLEHL GNAYQNPKAL VLAKTLDQAT GKFLDENKSP ARKVGGLDNR GSHFYLCLYW AQALAEQTED AALKAQFEDV AKALSDNEAR ILEELAAAQG KPQAIGGYYR PNVDLTSQAM RPSATLNGIV DAVA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA CCACCATGTC CACTTCGCCC AAGATCATCT ACACCCTCAC CGACGAAGCG CCGGCGCTCG CGACCTATTC GCTGCTGCCG ATCGTCAAGG CCTTCACGCG TTCGTCCGGC GTCGCCGTCG AAACGCGCGA CATCTCGCTC GCCGGCCGCA TCATCGCGGC ATTCGCAGAC GTCCTGCCGT CGGAGCAGAA GGGTTCCGAC GATCTGGCCG AGCTGGGCCA GCTCACGCTG AAGCCGGAAG CGAACATCAT CAAGCTGCCG AACATCAGCG CATCGGTGCC GCAGCTGAAG GCCGCGATCG CCGAACTGCA GGCGCAGGGC TACGCGCTGC CGGCCTATCC GGAAGATCCG TCGACGGACG AAGAGAAGGC CGTCAAGGCG CGCTACGACA AGATCAAGGG CAGCGCGGTG AACCCGGTGC TGCGCGAAGG CAACTCCGAC CGCCGCGCGC CGCTGTCGGT CAAGAACTAC GCACGCAAGC ATCCGCACAA GATGGGCGCG TGGAAGTCCA CGTCGAAGGC CCACGTCGCG CACATGAGCG AAGGCGACTT CTACGGCAGC GAGAAGTCGG CGCTGATCGC CGAGGCCGGC AGCGTGAAGA TCGAACTCAC GACGACCGAC GGCGCGAAGA AGGTGCTGAA GGAAAAGACG GCCGTGAAGG CCGGTGAAGT GATCGACGCA TCGGTGATGA GCCGCAACGC GCTGCGCAGC TTCATCGAAG CGCAGATCGC GGACGCGAAG GCGCAGGACG TGCTGTTCTC GGTGCACCTG AAGGCGACCA TGATGAAGGT CTCGGACCCG ATCCTGTTCG GTCACTTCGT GTCGGTGTTC TACCGCGACG CGCTCACGAA GCACGCGGAC GTGCTGGCGA AGGCCGGCTT CAATCCGAAC AACGGGATTG GCGACCTGTA CGCGCGCCTG AAGGACCTGC CGGCCGACAC GCGCGAAGCG ATCGAGGCCG ACGTCAAGGC CGAGTACGCG GTGCGCCCGC GCCTCGCGAT GGTCAACTCG GACAAGGGCA TCACGAACCT GCACGTGCCG AGCGACGTGA TCGTCGACGC ATCGATGCCG GCGATGATCC GCGATTCGGG CGGCATGTGG GGCCCGGACG GTCAACTGTA CGACGCGAAG GCCGTGATTC CGGACCGCTG CTACGCAGGC GTGTACCAGG CCGTGATCGA GGACTGCAAG CAGCACGGCG CGTTCGATCC GGTCACGATG GGCAGCGTGC CGAACGTAGG CCTGATGGCG CAGGCGGCCG AAGAATACGG TTCGCACGAC AAGACGTTCC AGATCCCGGC GGACGGCGTC GTGCGCGTCA CCGACGAAGC CGGCAACGTG CTGCTCGAGC ACGCGGTCGA GGCGGGCGAC ATCTGGCGCA TGTGCCAGAC GAAGGACGCA CCGGTGCAGG ACTGGGTCAA GCTCGCGGTC AACCGCGCCC GCGCGACCGG CGCCCCGGCC GTGTTCTGGC TCGATCCGGC GCGCGCGCAC GATGCGCAGA TCATCGCGAA GGTCGAACGC TACCTGAAGG ATCACGACAC GAACGGCCTC GACATCCGGA TCATGACGCC GGTCGACGCC ACGCGTTTCT CGCTCGAGCG CATCCGCGCG GGCAAGGACA CGATTTCGGT CACCGGCAAC GTGCTGCGCG ATTACCTGAC CGATCTGTTC CCGATCATGG AACTCGGCAC CAGCGCGAAG ATGCTGTCGA TCGTGCCGCT GATGGCCGGC GGCGGGATGT TCGAAACGGG GGCGGGCGGC TCCGCGCCGA AGCACGTGCA GCAGTTCGTC GAGGAAGGCT TCCTGCGCTG GGATTCGCTC GGCGAATTCC TCGCGCTGGC CGCGTCGCTC GAGCACCTCG GCAACGCGTA CCAGAACCCG AAGGCGCTCG TGCTCGCGAA GACGCTCGAC CAGGCAACCG GCAAGTTCCT GGACGAGAAC AAGTCGCCGG CGCGCAAGGT CGGCGGCCTC GACAACCGCG GCAGCCACTT CTACCTGTGC CTGTACTGGG CGCAGGCATT GGCCGAGCAG ACCGAGGACG CCGCGCTGAA GGCGCAGTTC GAGGACGTGG CGAAGGCGCT GTCCGACAAC GAAGCGCGCA TCCTGGAGGA ACTGGCGGCC GCGCAGGGCA AGCCGCAGGC GATCGGCGGC TACTACCGTC CGAACGTCGA CCTGACGAGC CAGGCCATGC GCCCGAGCGC GACGCTGAAC GGCATCGTCG ACGCGGTCGC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT