BuceA.18451.a

Beta-hexosaminidase 1 (EC 3.2.1.52) (Beta-hexosaminidase 2) (EC 3.2.1.52)

CENTER ID: BuceA.18451.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.18451.a.B1.GE35023 full length 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.18451.a.B1.PW36254 full length 1 342
Structures
4G6C
DEPOSITED: 7/18/2012
DETERMINATION: XRay
CLONE: BuceA.18451.a.B1.GE35023
PROTEIN: BuceA.18451.a.B1.PW36254
4GNV
DEPOSITED: 8/17/2012
DETERMINATION: XRay
CLONE: BuceA.18451.a.B1.GE35023
PROTEIN: BuceA.18451.a.B1.PW36254
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_130549
PATRIC ID: fig|216591.5.peg.2964
RefSeq: YP_002230164.1
UniProt: B4EA43
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTTPGPVML DVVGTTLSRD DARRLAHPNT GGVILFARHF QNRAQLTALT DSIRAVREDI LIAVDHEGGR VQRFRTDGFT VLPAMRRLGE LWDRDVLLAT KVATAVGYIL AAELRACGID MSFTPVLDLD YGHSKVIGDR AFHRDPRVVT LLAKSLNHGL SLAGMANCGK HFPGHGFAEA DSHVALPTDD RTLDAILEQD VAPYDWLGLS LAAVIPAHVI YTQVDKRPAG FSRVWLQDIL RGKLGFTGAI FSDDLSMEAA REGGTLTQAA DAALAAGCDM VLVCNQPDAA EVVLNGLKAR ASAESVRRIK RMRARGKALK WDKLIAQPEY LQAQALLSSA LA
NT Sequence
atgaaaacga ctcccggccc ggtcatgctc gacgtcgtcg gcacgaccct gtcgcgcgac gatgcgcggc gcctcgcgca tccgaatacc ggcggcgtga tcctgttcgc gcggcacttc cagaatcgtg cgcaactgac cgcgctgacc gattcgatcc gcgcggtgcg cgaagacatc ctgatcgccg tcgatcacga aggcgggcgc gtgcagcggt tccgcaccga cggcttcacg gtgctgcccg cgatgcgtcg cctcggcgag ctgtgggatc gcgacgtgct gctcgcgacg aaggtcgcga ccgccgtcgg ttacatcctg gccgccgagc tgcgcgcgtg cgggatcgac atgagcttca cgcccgtcct cgatctcgac tacgggcact cgaaggtgat cggcgatcgt gcgttccatc gcgatccgcg cgtcgtcacg ctgctggcga agagcctgaa ccatgggctg tcgcttgccg gcatggcaaa ctgcggcaag cattttcccg ggcacggctt cgcggaggcc gattcgcacg tcgcgctgcc gaccgacgac cgcacgctcg acgcgatcct cgagcaggac gtcgcgccgt acgactggct cgggctatcg ctggccgcgg tgatcccggc gcacgtgatc tacacgcagg tcgacaagcg gccggcgggc ttttcgcgcg tgtggctgca ggacatcctg cgcggcaagc tcggcttcac gggtgcgatc ttcagcgacg acctgtcgat ggaggctgcc cgcgaaggcg gcacgctcac gcaggccgcc gacgccgcgc tcgccgccgg ttgcgacatg gtgctcgtct gcaatcagcc ggatgcagcc gaagtcgtgc tgaacggact gaaggcgcgg gcctcggccg agtcggtgcg gcgcatcaag cggatgcgtg cgcgcggcaa ggcgctcaag tgggacaagc tgatcgcgca gcccgagtat ctgcaggcac aggcgctgtt gagcagcgcg ctggcgtaa
Details for BuceA.18451.a.B1.GE35023
HARVESTED ON: 3/19/2012
SEQUENCED ON: 2/4/2013
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK TTPGPVMLDV VGTTLSRDDA RRLAHPNTGG VILFARHFQN RAQLTALTDS IRAVREDILI AVDHEGGRVQ RFRTDGFTVL PAMRRLGELW DRDVLLATKV ATAVGYILAA ELRACGIDMS FTPVLDLDYG HSKVIGDRAF HRDPRVVTLL AKSLNHGLSL AGMANCGKHF PGHGFAEADS HVALPTDDRT LDAILEQDVA PYDWLGLSLA AVIPAHVIYT QVDKRPAGFS RVWLQDILRG KLGFTGAIFS DDLSMEAARE GGTLTQAADA ALAAGCDMVL VCNQPDAAEV VLNGLKARAS AESVRRIKRM RARGKALKWD KLIAQPEYLQ AQALLSSALA
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa acgactcccg gcccggtcat gctcgacgtc gtcggcacga ccctgtcgcg cgacgatgcg cggcgcctcg cgcatccgaa taccggcggc gtgatcctgt tcgcgcggca cttccagaat cgtgcgcaac tgaccgcgct gaccgattcg atccgcgcgg tgcgcgaaga catcctgatc gccgtcgatc acgaaggcgg gcgcgtgcag cggttccgca ccgacggctt cacggtgctg cccgcgatgc gtcgcctcgg cgagctgtgg gatcgcgacg tgctgctcgc gacgaaggtc gcgaccgccg tcggttacat cctggccgcc gagctgcgcg cgtgcgggat cgacatgagc ttcacgcccg tcctcgatct cgactacggg cactcgaagg tgatcggcga tcgtgcgttc catcgcgatc cgcgcgtcgt cacgctgctg gcgaagagcc tgaaccatgg gctgtcgctt gccggcatgg caaactgcgg caagcatttt cccgggcacg gcttcgcgga ggccgattcg cacgtcgcgc tgccgaccga cgaccgcacg ctcgacgcga tcctcgagca ggacgtcgcg ccgtacgact ggctcgggct atcgctggcc gcggtgatcc cggcgcacgt gatctacacg caggtcgaca agcggccggc gggcttttcg cgcgtgtggc tgcaggacat cctgcgcggc aagctcggct tcacgggtgc gatcttcagc gacgacctgt cgatggaggc tgcccgcgaa ggcggcacgc tcacgcaggc cgccgacgcc gcgctcgccg ccggttgcga catggtgctc gtctgcaatc agccggatgc agccgaagtc gtgctgaacg gactgaaggc gcgggcctcg gccgagtcgg tgcggcgcat caagcggatg cgtgcgcgcg gcaaggcgct caagtgggac aagctgatcg cgcagcccga gtatctgcag gcacaggcgc tgttgagcag cgcgctggcg taagtgagta aga
Expected Protein Sequence
MAHHHHHHMK TTPGPVMLDV VGTTLSRDDA RRLAHPNTGG VILFARHFQN RAQLTALTDS IRAVREDILI AVDHEGGRVQ RFRTDGFTVL PAMRRLGELW DRDVLLATKV ATAVGYILAA ELRACGIDMS FTPVLDLDYG HSKVIGDRAF HRDPRVVTLL AKSLNHGLSL AGMANCGKHF PGHGFAEADS HVALPTDDRT LDAILEQDVA PYDWLGLSLA AVIPAHVIYT QVDKRPAGFS RVWLQDILRG KLGFTGAIFS DDLSMEAARE GGTLTQAADA ALAAGCDMVL VCNQPDAAEV VLNGLKARAS AESVRRIKRM RARGKALKWD KLIAQPEYLQ AQALLSSALA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa cgactcccgg cccggtcatg ctcgacgtcg tcggcacgac cctgtcgcgc gacgatgcgc ggcgcctcgc gcatccgaat accggcggcg tgatcctgtt cgcgcggcac ttccagaatc gtgcgcaact gaccgcgctg accgattcga tccgcgcggt gcgcgaagac atcctgatcg ccgtcgatca cgaaggcggg cgcgtgcagc ggttccgcac cgacggcttc acggtgctgc ccgcgatgcg tcgcctcggc gagctgtggg atcgcgacgt gctgctcgcg acgaaggtcg cgaccgccgt cggttacatc ctggccgccg agctgcgcgc gtgcgggatc gacatgagct tcacgcccgt cctcgatctc gactacgggc actcgaaggt gatcggcgat cgtgcgttcc atcgcgatcc gcgcgtcgtc acgctgctgg cgaagagcct gaaccatggg ctgtcgcttg ccggcatggc aaactgcggc aagcattttc ccgggcacgg cttcgcggag gccgattcgc acgtcgcgct gccgaccgac gaccgcacgc tcgacgcgat cctcgagcag gacgtcgcgc cgtacgactg gctcgggcta tcgctggccg cggtgatccc ggcgcacgtg atctacacgc aggtcgacaa gcggccggcg ggcttttcgc gcgtgtggct gcaggacatc ctgcgcggca agctcggctt cacgggtgcg atcttcagcg acgacctgtc gatggaggct gcccgcgaag gcggcacgct cacgcaggcc gccgacgccg cgctcgccgc cggttgcgac atggtgctcg tctgcaatca gccggatgca gccgaagtcg tgctgaacgg actgaaggcg cgggcctcgg ccgagtcggt gcggcgcatc aagcggatgc gtgcgcgcgg caaggcgctc aagtgggaca agctgatcgc gcagcccgag tatctgcagg cacaggcgct gttgagcagc gcgctggcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuceA.18451.a.B1.PW36254
PURIFICATION DATe: 5/11/2012
CONCENTRATION: 33.69mg/ml
OBSERVED MW: 34kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK TTPGPVMLDV VGTTLSRDDA RRLAHPNTGG VILFARHFQN RAQLTALTDS IRAVREDILI AVDHEGGRVQ RFRTDGFTVL PAMRRLGELW DRDVLLATKV ATAVGYILAA ELRACGIDMS FTPVLDLDYG HSKVIGDRAF HRDPRVVTLL AKSLNHGLSL AGMANCGKHF PGHGFAEADS HVALPTDDRT LDAILEQDVA PYDWLGLSLA AVIPAHVIYT QVDKRPAGFS RVWLQDILRG KLGFTGAIFS DDLSMEAARE GGTLTQAADA ALAAGCDMVL VCNQPDAAEV VLNGLKARAS AESVRRIKRM RARGKALKWD KLIAQPEYLQ AQALLSSALA
Validated NT Sequence
atggctcacc accaccacca ccatatgaaa acgactcccg gcccggtcat gctcgacgtc gtcggcacga ccctgtcgcg cgacgatgcg cggcgcctcg cgcatccgaa taccggcggc gtgatcctgt tcgcgcggca cttccagaat cgtgcgcaac tgaccgcgct gaccgattcg atccgcgcgg tgcgcgaaga catcctgatc gccgtcgatc acgaaggcgg gcgcgtgcag cggttccgca ccgacggctt cacggtgctg cccgcgatgc gtcgcctcgg cgagctgtgg gatcgcgacg tgctgctcgc gacgaaggtc gcgaccgccg tcggttacat cctggccgcc gagctgcgcg cgtgcgggat cgacatgagc ttcacgcccg tcctcgatct cgactacggg cactcgaagg tgatcggcga tcgtgcgttc catcgcgatc cgcgcgtcgt cacgctgctg gcgaagagcc tgaaccatgg gctgtcgctt gccggcatgg caaactgcgg caagcatttt cccgggcacg gcttcgcgga ggccgattcg cacgtcgcgc tgccgaccga cgaccgcacg ctcgacgcga tcctcgagca ggacgtcgcg ccgtacgact ggctcgggct atcgctggcc gcggtgatcc cggcgcacgt gatctacacg caggtcgaca agcggccggc gggcttttcg cgcgtgtggc tgcaggacat cctgcgcggc aagctcggct tcacgggtgc gatcttcagc gacgacctgt cgatggaggc tgcccgcgaa ggcggcacgc tcacgcaggc cgccgacgcc gcgctcgccg ccggttgcga catggtgctc gtctgcaatc agccggatgc agccgaagtc gtgctgaacg gactgaaggc gcgggcctcg gccgagtcgg tgcggcgcat caagcggatg cgtgcgcgcg gcaaggcgct caagtgggac aagctgatcg cgcagcccga gtatctgcag gcacaggcgc tgttgagcag cgcgctggcg taagtgagta aga
Expressed Protein Sequence
MAHHHHHHMK TTPGPVMLDV VGTTLSRDDA RRLAHPNTGG VILFARHFQN RAQLTALTDS IRAVREDILI AVDHEGGRVQ RFRTDGFTVL PAMRRLGELW DRDVLLATKV ATAVGYILAA ELRACGIDMS FTPVLDLDYG HSKVIGDRAF HRDPRVVTLL AKSLNHGLSL AGMANCGKHF PGHGFAEADS HVALPTDDRT LDAILEQDVA PYDWLGLSLA AVIPAHVIYT QVDKRPAGFS RVWLQDILRG KLGFTGAIFS DDLSMEAARE GGTLTQAADA ALAAGCDMVL VCNQPDAAEV VLNGLKARAS AESVRRIKRM RARGKALKWD KLIAQPEYLQ AQALLSSALA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAA CGACTCCCGG CCCGGTCATG CTCGACGTCG TCGGCACGAC CCTGTCGCGC GACGATGCGC GGCGCCTCGC GCATCCGAAT ACCGGCGGCG TGATCCTGTT CGCGCGGCAC TTCCAGAATC GTGCGCAACT GACCGCGCTG ACCGATTCGA TCCGCGCGGT GCGCGAAGAC ATCCTGATCG CCGTCGATCA CGAAGGCGGG CGCGTGCAGC GGTTCCGCAC CGACGGCTTC ACGGTGCTGC CCGCGATGCG TCGCCTCGGC GAGCTGTGGG ATCGCGACGT GCTGCTCGCG ACGAAGGTCG CGACCGCCGT CGGTTACATC CTGGCCGCCG AGCTGCGCGC GTGCGGGATC GACATGAGCT TCACGCCCGT CCTCGATCTC GACTACGGGC ACTCGAAGGT GATCGGCGAT CGTGCGTTCC ATCGCGATCC GCGCGTCGTC ACGCTGCTGG CGAAGAGCCT GAACCATGGG CTGTCGCTTG CCGGCATGGC AAACTGCGGC AAGCATTTTC CCGGGCACGG CTTCGCGGAG GCCGATTCGC ACGTCGCGCT GCCGACCGAC GACCGCACGC TCGACGCGAT CCTCGAGCAG GACGTCGCGC CGTACGACTG GCTCGGGCTA TCGCTGGCCG CGGTGATCCC GGCGCACGTG ATCTACACGC AGGTCGACAA GCGGCCGGCG GGCTTTTCGC GCGTGTGGCT GCAGGACATC CTGCGCGGCA AGCTCGGCTT CACGGGTGCG ATCTTCAGCG ACGACCTGTC GATGGAGGCT GCCCGCGAAG GCGGCACGCT CACGCAGGCC GCCGACGCCG CGCTCGCCGC CGGTTGCGAC ATGGTGCTCG TCTGCAATCA GCCGGATGCA GCCGAAGTCG TGCTGAACGG ACTGAAGGCG CGGGCCTCGG CCGAGTCGGT GCGGCGCATC AAGCGGATGC GTGCGCGCGG CAAGGCGCTC AAGTGGGACA AGCTGATCGC GCAGCCCGAG TATCTGCAGG CACAGGCGCT GTTGAGCAGC GCGCTGGCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT