SSGCID: Target Page

BuceA.18447.a

Ribosomal RNA small subunit methyltransferase B

CENTER ID:	BuceA.18447.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18447.a.B1.GE35037	full length		1	463

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.18447.a.B1.PS01504	full length		1	463

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.489
RefSeq:	YP_002229631.1
UniProt:	B4E7W1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRPRSSAPT SSGRPARLAA LHLAPDSLGF ALDAAAQAVD AVRRGTALPA ALSTVFAQMP SGAQALARGA TQDVAYRTMR RLGSADWLIG KFVSKAPPAH VHAVLAGAFA LLLDPPDEAA YPAFTVVDQA VTVIGARREF AFAKGMVNAV LRRFLRERDA LVAAMQDDPV ACWNYRAWWV DAVKRAWPDA WQAILAAGER QGPLTLRVNA RRANVDAYLD TLRASGIEAV AIGRHAVRLA SALPVDRIPG FADGVVSVQD AGAQLAAEWL GARDGMRVLD ACAAPGGKTG HILELADAEV VALESDATRA ARIGENLVRL SLAANVCVGD AGAPDAWYDG RPFDRILADV PCSASGIVRR HPDIRWLRRE ADIPALVAEQ RRILSALWPL VKPGGELLYV TCSIFPEEGE GQARWFEAAC EDAVRLDAPG QLLPGGVQGR AAAGALDQNT DHDGFFYARF QKR

NT Sequence

atgacacgac cccgttcttc cgctccgact tcttccggcc gcccggcgcg cctggctgcg ctgcacctgg cgcccgattc gctcggcttc gcgctcgacg cggccgcgca ggcggtcgat gcggtgcgac gcggcaccgc gttgccggcg gcactgtcga cggtattcgc gcagatgccg tccggcgcgc aggcactcgc acgcggcgcg acgcaggacg tcgcctaccg gacgatgcgt cgcctcggca gcgcggactg gctgatcggc aagttcgtca gcaaggcgcc gcccgcgcac gtgcacgcgg tgctcgccgg cgcgttcgcg ctgctgctcg acccgcctga cgaagctgcg tacccggcgt tcacggtggt cgaccaggcc gtcaccgtca tcggtgcgcg gcgcgaattc gcgttcgcga aggggatggt caacgcggtg ctgcgccgtt tcctgcgcga gcgcgacgca ctcgtcgcgg cgatgcagga cgatccggtt gcgtgctgga actaccgtgc gtggtgggtc gatgcggtga agcgcgcatg gcccgacgcg tggcaggcga tcctcgcggc cggcgagcgc caggggccgc tgacgctgcg cgtgaacgcg cgtcgcgcga acgtcgatgc gtatctcgac acgctgcgtg cgagcggaat cgaagcggtc gcgatcggcc ggcatgcggt gcggctcgcg tcggcgctgc cggtcgaccg cattccgggc ttcgccgacg gcgtggtgtc ggtgcaggac gccggcgcgc agctcgcggc cgaatggctc ggcgcgcgcg acggcatgcg cgtgctcgac gcgtgcgcgg cgcccggcgg caagaccggc catattctcg aactcgccga cgcggaagtc gtggcgctcg aaagcgacgc gacgcgcgcg gcgcgcatcg gcgagaatct cgtgcgcctg tcgctcgcgg cgaacgtgtg cgtcggcgac gcgggcgcgc cggacgcgtg gtacgacggg cgtccgttcg atcgcatcct ggccgacgtg ccgtgctcgg cgtccggcat cgtgcggcgg caccccgaca tccgctggct gcgccgcgag gccgacatcc cggcgctggt cgcggagcag cgtcgcatcc tgtcggcgct gtggccgctc gtgaagccgg gcggcgaact gctttacgtg acctgttcga tcttccccga agaaggcgaa gggcaggccc gctggtttga agcggcctgt gaagatgcgg tacgattgga cgcccccggc caactgctgc cgggcggcgt gcagggacgg gcggccgccg gcgcactcga ccagaacacc gatcacgacg gatttttcta cgcgcggttt cagaaacggt ga

Details for BuceA.18447.a.B1.GE35037

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	27
PERCENT COVERAGE:	37

Validated AA Sequence

MAHHHHHHXI QXGDAXPDAQ LXEFXXXAXE GCTLGPNACS VRDQVAGKRV VXFGXPGAFT PTCSAQHVPG YVEHAEQLRA AGXDEIWCVS VNDAXVMGAW GRDXHTAGKV RMMADGXAAF THALGLTQDL SARGMGIRSL RYAMVIDGGV VKTLAVEAPG KFEVSDAASV XATXTS

Validated NT Sequence

atcttactca cttaggacgt cnacgtcgca ngaacactcg ccgcatcgct cacttcgaat ttgcccggcg cctcgacggc cagcgtcttg accacaccgc cgtcaatcac catcgcgtag cgcagggaac gaattcccat gccacgcgcg gacaaatcct gcgtcagccc cagcgcatga gtgaaagccg cgntgccgtc cgccatcatg cgcaccttgc ccgcggtgtg canatcacgt ccccatgcnc ccatcacnaa ngcgtcgttg acggacacgc accagatctc gtcnatcccc gccgcgcgca attgctcggc gtgttcgacg tanccnggca catgctgcgc cgagcaggtc ggcgtgaaag cgcccggnaa tccgaanatc accacccgct ttcccgccac ctggtcgcgc acgctgcang cgttcggccc cagcgtgcac ccttcnngcg cgtnnnnnat gaactcnaac aattgcgcgt cnggcancgc gtcnccnnnt tgaatcanat ggtggtggtg gtggtgagcc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaan cgttgngntc tcnntntnnn gnnnnnnatn aannntcctg caccgacacc acgccgtcgg cgaagcccgg aatgcggtcg accggcagcg ccgacgcgag ccgcaccgca tgccggccga tcgcgaccgc ttcgattccg ctcgcacgca gcgtgtcgag atacgcatcg acgttcgcgc gacgcgcgtt cacgcgcagc gtcagcggcc cctggcgctc gccggccgcg aggatcgcct gccacgcgtc gggccatgcg cgcttcaccg catcgaccca ccacgcacgg tagttccagc acgcaaccgg atcgtcctgc atcgccgcga cgagtgcgtc gcgctcgcgc aggaaacggc gcagcaccgc gttgaccatc cccttcgcga acgcgaattc gcgccgcgca ccgatgacgg tgacggcctg gtcgac

Expected Protein Sequence

MAHHHHHHMT RPRSSAPTSS GRPARLAALH LAPDSLGFAL DAAAQAVDAV RRGTALPAAL STVFAQMPSG AQALARGATQ DVAYRTMRRL GSADWLIGKF VSKAPPAHVH AVLAGAFALL LDPPDEAAYP AFTVVDQAVT VIGARREFAF AKGMVNAVLR RFLRERDALV AAMQDDPVAC WNYRAWWVDA VKRAWPDAWQ AILAAGERQG PLTLRVNARR ANVDAYLDTL RASGIEAVAI GRHAVRLASA LPVDRIPGFA DGVVSVQDAG AQLAAEWLGA RDGMRVLDAC AAPGGKTGHI LELADAEVVA LESDATRAAR IGENLVRLSL AANVCVGDAG APDAWYDGRP FDRILADVPC SASGIVRRHP DIRWLRREAD IPALVAEQRR ILSALWPLVK PGGELLYVTC SIFPEEGEGQ ARWFEAACED AVRLDAPGQL LPGGVQGRAA AGALDQNTDH DGFFYARFQK R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac gaccccgttc ttccgctccg acttcttccg gccgcccggc gcgcctggct gcgctgcacc tggcgcccga ttcgctcggc ttcgcgctcg acgcggccgc gcaggcggtc gatgcggtgc gacgcggcac cgcgttgccg gcggcactgt cgacggtatt cgcgcagatg ccgtccggcg cgcaggcact cgcacgcggc gcgacgcagg acgtcgccta ccggacgatg cgtcgcctcg gcagcgcgga ctggctgatc ggcaagttcg tcagcaaggc gccgcccgcg cacgtgcacg cggtgctcgc cggcgcgttc gcgctgctgc tcgacccgcc tgacgaagct gcgtacccgg cgttcacggt ggtcgaccag gccgtcaccg tcatcggtgc gcggcgcgaa ttcgcgttcg cgaaggggat ggtcaacgcg gtgctgcgcc gtttcctgcg cgagcgcgac gcactcgtcg cggcgatgca ggacgatccg gttgcgtgct ggaactaccg tgcgtggtgg gtcgatgcgg tgaagcgcgc atggcccgac gcgtggcagg cgatcctcgc ggccggcgag cgccaggggc cgctgacgct gcgcgtgaac gcgcgtcgcg cgaacgtcga tgcgtatctc gacacgctgc gtgcgagcgg aatcgaagcg gtcgcgatcg gccggcatgc ggtgcggctc gcgtcggcgc tgccggtcga ccgcattccg ggcttcgccg acggcgtggt gtcggtgcag gacgccggcg cgcagctcgc ggccgaatgg ctcggcgcgc gcgacggcat gcgcgtgctc gacgcgtgcg cggcgcccgg cggcaagacc ggccatattc tcgaactcgc cgacgcggaa gtcgtggcgc tcgaaagcga cgcgacgcgc gcggcgcgca tcggcgagaa tctcgtgcgc ctgtcgctcg cggcgaacgt gtgcgtcggc gacgcgggcg cgccggacgc gtggtacgac gggcgtccgt tcgatcgcat cctggccgac gtgccgtgct cggcgtccgg catcgtgcgg cggcaccccg acatccgctg gctgcgccgc gaggccgaca tcccggcgct ggtcgcggag cagcgtcgca tcctgtcggc gctgtggccg ctcgtgaagc cgggcggcga actgctttac gtgacctgtt cgatcttccc cgaagaaggc gaagggcagg cccgctggtt tgaagcggcc tgtgaagatg cggtacgatt ggacgccccc ggccaactgc tgccgggcgg cgtgcaggga cgggcggccg ccggcgcact cgaccagaac accgatcacg acggattttt ctacgcgcgg tttcagaaac ggtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for BuceA.18447.a.B1.PS01504

PURIFICATION DATe:	5/23/2012
CONCENTRATION:	51.44mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	27
PERCENT COVERAGE:	37

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHXI QXGDAXPDAQ LXEFXXXAXE GCTLGPNACS VRDQVAGKRV VXFGXPGAFT PTCSAQHVPG YVEHAEQLRA AGXDEIWCVS VNDAXVMGAW GRDXHTAGKV RMMADGXAAF THALGLTQDL SARGMGIRSL RYAMVIDGGV VKTLAVEAPG KFEVSDAASV XATXTS

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC GACCCCGTTC TTCCGCTCCG ACTTCTTCCG GCCGCCCGGC GCGCCTGGCT GCGCTGCACC TGGCGCCCGA TTCGCTCGGC TTCGCGCTCG ACGCGGCCGC GCAGGCGGTC GATGCGGTGC GACGCGGCAC CGCGTTGCCG GCGGCACTGT CGACGGTATT CGCGCAGATG CCGTCCGGCG CGCAGGCACT CGCACGCGGC GCGACGCAGG ACGTCGCCTA CCGGACGATG CGTCGCCTCG GCAGCGCGGA CTGGCTGATC GGCAAGTTCG TCAGCAAGGC GCCGCCCGCG CACGTGCACG CGGTGCTCGC CGGCGCGTTC GCGCTGCTGC TCGACCCGCC TGACGAAGCT GCGTACCCGG CGTTCACGGT GGTCGACCAG GCCGTCACCG TCATCGGTGC GCGGCGCGAA TTCGCGTTCG CGAAGGGGAT GGTCAACGCG GTGCTGCGCC GTTTCCTGCG CGAGCGCGAC GCACTCGTCG CGGCGATGCA GGACGATCCG GTTGCGTGCT GGAACTACCG TGCGTGGTGG GTCGATGCGG TGAAGCGCGC ATGGCCCGAC GCGTGGCAGG CGATCCTCGC GGCCGGCGAG CGCCAGGGGC CGCTGACGCT GCGCGTGAAC GCGCGTCGCG CGAACGTCGA TGCGTATCTC GACACGCTGC GTGCGAGCGG AATCGAAGCG GTCGCGATCG GCCGGCATGC GGTGCGGCTC GCGTCGGCGC TGCCGGTCGA CCGCATTCCG GGCTTCGCCG ACGGCGTGGT GTCGGTGCAG GACGCCGGCG CGCAGCTCGC GGCCGAATGG CTCGGCGCGC GCGACGGCAT GCGCGTGCTC GACGCGTGCG CGGCGCCCGG CGGCAAGACC GGCCATATTC TCGAACTCGC CGACGCGGAA GTCGTGGCGC TCGAAAGCGA CGCGACGCGC GCGGCGCGCA TCGGCGAGAA TCTCGTGCGC CTGTCGCTCG CGGCGAACGT GTGCGTCGGC GACGCGGGCG CGCCGGACGC GTGGTACGAC GGGCGTCCGT TCGATCGCAT CCTGGCCGAC GTGCCGTGCT CGGCGTCCGG CATCGTGCGG CGGCACCCCG ACATCCGCTG GCTGCGCCGC GAGGCCGACA TCCCGGCGCT GGTCGCGGAG CAGCGTCGCA TCCTGTCGGC GCTGTGGCCG CTCGTGAAGC CGGGCGGCGA ACTGCTTTAC GTGACCTGTT CGATCTTCCC CGAAGAAGGC GAAGGGCAGG CCCGCTGGTT TGAAGCGGCC TGTGAAGATG CGGTACGATT GGACGCCCCC GGCCAACTGC TGCCGGGCGG CGTGCAGGGA CGGGCGGCCG CCGGCGCACT CGACCAGAAC ACCGATCACG ACGGATTTTT CTACGCGCGG TTTCAGAAAC GGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT